FastQCFastQC Report
Mon 15 Aug 2016
SRR2921545_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921545_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563225
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26620.1702889859105375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23680.15148171248540676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22000.081.564841
GTATCAA46850.061.206091
TCAACGC61650.046.077114
CAACGCA63850.044.270345
ATCAACG64700.043.9050063
TATCAAC65400.043.7082522
AACGCAG66500.042.586136
ACGCAGA80000.035.469557
GTACATG79450.034.216071
CGCAGAG85650.033.0592468
TACATGG82450.032.5751042
ACATGGG84900.031.2142033
GCAGAGT90200.030.4673679
CATGGGG74000.029.212914
ACCGTAT2256.5701897E-926.4381398
AGAGTAC84750.026.4260210-11
GTGGTAT7250.026.3130471
CAGAGTA87750.025.69203610-11
TACCGTA2109.8358214E-825.4947367
GAGTACT49650.024.62101612-13
Produced by FastQC (version 0.11.5)