##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921545_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1563225
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31286986838107	33.0	31.0	34.0	28.0	34.0
2	31.383266644277054	33.0	31.0	34.0	28.0	34.0
3	31.45946616769819	33.0	31.0	34.0	28.0	34.0
4	34.955541268851256	37.0	35.0	37.0	32.0	37.0
5	34.619056757664445	37.0	35.0	37.0	31.0	37.0
6	34.59681299876857	37.0	35.0	37.0	31.0	37.0
7	34.44736618209151	37.0	35.0	37.0	30.0	37.0
8	34.42318092405124	37.0	35.0	37.0	30.0	37.0
9	36.00439284172144	39.0	37.0	39.0	30.0	39.0
10-11	35.87940763485743	39.0	37.0	39.0	30.0	39.0
12-13	35.786803882998285	39.0	37.0	39.0	30.0	39.0
14-15	36.97856642517871	40.0	37.0	41.0	30.0	41.0
16-17	36.951825233091846	40.0	37.0	41.0	30.0	41.0
18-19	36.9680039661597	40.0	37.0	41.0	30.0	41.0
20-21	36.95333877081035	40.0	37.0	41.0	30.0	41.0
22-23	36.888069855587005	40.0	37.0	41.0	30.0	41.0
24-25	36.821835308416894	40.0	37.0	41.0	30.0	41.0
26-27	36.69547410001759	40.0	36.5	41.0	30.0	41.0
28-29	36.5013756816837	40.0	36.0	41.0	29.5	41.0
30-31	36.45343632554494	40.0	36.0	41.0	30.0	41.0
32-33	36.32707415759087	40.0	35.5	41.0	29.0	41.0
34-35	36.20321834668714	40.0	35.0	41.0	29.0	41.0
36-37	36.06407203057781	40.0	35.0	41.0	28.0	41.0
38-39	35.891666586703764	40.0	35.0	41.0	27.5	41.0
40-41	35.68727374498233	40.0	35.0	41.0	27.0	41.0
42-43	35.51116857777991	39.0	35.0	41.0	26.0	41.0
44-45	35.27971469238273	39.0	35.0	41.0	25.5	41.0
46-47	34.98180236370324	39.0	35.0	41.0	24.0	41.0
48-49	34.703820627228964	39.0	34.0	41.0	23.0	41.0
50-51	34.46500695677206	39.0	34.0	41.0	23.0	41.0
52-53	34.255067568648144	38.0	34.0	41.0	22.0	41.0
54-55	34.03146124198372	38.0	34.0	40.0	22.0	41.0
56-57	33.75869532536903	37.5	33.0	40.0	20.5	41.0
58-59	33.60746661549041	37.0	33.0	40.0	20.5	41.0
60-61	33.47899502630779	37.0	33.0	40.0	20.5	41.0
62-63	33.20714068672136	36.0	33.0	40.0	20.0	41.0
64-65	32.903508452078235	36.0	33.0	39.0	19.5	41.0
66-67	32.596443250331845	35.0	33.0	39.0	18.5	41.0
68-69	32.2773119672472	35.0	33.0	38.5	18.0	40.5
70-71	31.97718818468231	35.0	32.0	37.0	17.5	40.0
72-73	31.668843256728877	35.0	32.0	37.0	16.0	39.0
74-75	31.389417070479297	35.0	32.0	36.0	13.5	39.0
76-77	31.11300708471269	35.0	32.0	36.0	12.5	38.5
78-79	30.840716787410642	35.0	32.0	35.5	10.5	37.0
80-81	30.634096499224363	35.0	31.0	35.0	8.0	37.0
82-83	30.459725567336754	35.0	31.0	35.0	7.0	36.5
84-85	30.279892849717733	35.0	31.0	35.0	7.0	36.0
86-87	30.147027139407314	35.0	31.0	35.0	7.0	36.0
88-89	30.046368884837435	35.0	31.0	35.0	6.0	35.5
90-91	29.95800028786643	35.0	31.0	35.0	3.5	35.0
92-93	30.027892657806778	35.0	31.0	35.0	2.0	35.0
94-95	30.001557677237763	35.0	31.0	35.0	2.0	35.0
96-97	29.987058804714614	35.0	31.0	35.0	2.0	35.0
98-99	29.951606134753476	35.0	31.0	35.0	2.0	35.0
100-101	29.904419709254906	35.0	31.0	35.0	2.0	35.0
102-103	29.03103935773801	33.5	29.5	35.0	2.0	35.0
104-105	29.7326162260711	34.5	31.0	35.0	2.0	35.0
106-107	29.840867117657407	35.0	31.0	35.0	2.0	35.0
108-109	29.846309712293497	35.0	32.0	35.0	2.0	35.0
110-111	29.813296870252202	35.0	32.0	35.0	2.0	35.0
112-113	29.768165491212077	35.0	31.5	35.0	2.0	35.0
114-115	29.70775384221721	35.0	31.0	35.0	2.0	35.0
116-117	29.63512290297302	35.0	31.0	35.0	2.0	35.0
118-119	29.542385453149738	35.0	31.0	35.0	2.0	35.0
120-121	29.422751363367396	35.0	31.0	35.0	2.0	35.0
122-123	29.305143533400503	35.0	31.0	35.0	2.0	35.0
124-125	28.388934414431702	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25641.0
3	9614.0
4	5428.0
5	4557.0
6	4916.0
7	5651.0
8	6447.0
9	7166.0
10	8296.0
11	8860.0
12	9276.0
13	10978.0
14	14299.0
15	11995.0
16	6415.0
17	5520.0
18	5084.0
19	4462.0
20	4238.0
21	4398.0
22	5387.0
23	7194.0
24	10106.0
25	14111.0
26	17500.0
27	19328.0
28	22716.0
29	27264.0
30	31527.0
31	38301.0
32	47556.0
33	61646.0
34	85747.0
35	139847.0
36	285105.0
37	450922.0
38	135462.0
39	265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.91848490942839	17.364885550942606	12.744522854584558	24.972106685044444
2	16.982791709314228	19.35209825997953	37.381141248720574	26.283968781985674
3	19.55695781367172	23.809374512117405	28.631574098009054	28.002093576201826
4	13.917448239532854	15.397662866173262	34.60684959301458	36.0780393012793
5	14.878907789728022	36.544968696729406	32.758885470470496	15.817238043072072
6	34.21847896400786	34.25994117229275	16.3898573712832	15.131722492416191
7	29.947119060491573	31.207688050443235	20.00211139867366	18.843081490391537
8	28.248742265508646	33.07438674248046	18.878236459202316	19.798634532808574
9	27.93620817491893	14.40043706701929	18.496788849673127	39.166565908388655
10-11	26.95884795119422	24.473456002472023	26.099462380209502	22.46823366612426
12-13	27.255562016880052	22.137777189221485	26.63303579253903	23.973625001359437
14-15	24.651125150975457	23.025004350140225	24.287600565006446	28.03626993387787
16-17	24.576385587801184	26.08956153949011	24.68719550464896	24.646857368059745
18-19	24.600400862628742	24.825302326295525	26.854949271621216	23.719347539454517
20-21	25.621253373743073	23.791106722086106	26.721132803515314	23.866507100655507
22-23	25.367746434494993	23.481935361076857	26.648625219320344	24.50169298510781
24-25	24.8874183329052	23.97310516184984	26.604142800611523	24.535333704633437
26-27	24.497569820089275	24.040387626011402	26.788662956406466	24.673379597492858
28-29	24.623751369594597	24.108223965706152	26.722906019818133	24.545118644881114
30-31	24.80951271856128	24.063272234927062	26.619717769129668	24.507497277381987
32-33	24.524162481821595	24.102212374942496	26.494371972531976	24.87925317070393
34-35	24.75142897545675	24.117867999971846	26.62510965662998	24.505593367941426
36-37	24.973447613100372	23.989345774779455	26.40071838602436	24.636488226095814
38-39	24.930535671152587	24.127388861072944	26.34080951337378	24.601265954400688
40-41	24.909903310401567	24.102951742210546	26.391116559173355	24.596028388214528
42-43	24.963341044722647	24.098336847816075	26.191799909920054	24.746522197541225
44-45	24.882591559388835	24.172126532388095	26.01380748854708	24.931474419675993
46-47	25.08118552784589	24.271649607388674	25.75958974555843	24.887575119207003
48-49	25.32359296487235	24.562735988195872	25.148239458474077	24.9654315884577
50-51	25.110656555833096	24.845611870930757	25.109536874001883	24.934194699234265
52-53	25.3311292178184	24.684323479013127	25.135491037435838	24.849056265732635
54-55	25.108139797140904	24.713362754993497	25.258948466240007	24.919548981625592
56-57	24.99240963466108	24.751661482183195	25.139673919824627	25.116254963331098
58-59	25.05904803341177	24.533814056679073	25.296455838341313	25.110682071567847
60-61	25.060929844778464	24.666269532262262	25.44640829042597	24.826392332533306
62-63	24.957945124225848	24.763107396219315	25.320010570506625	24.958936909048212
64-65	25.017035453578153	24.442532776852513	25.453942851731732	25.086488917837606
66-67	25.000359878044527	24.66485757306564	25.334230735888223	25.00055181300161
68-69	25.047862810340416	24.719733811108267	25.170335295623243	25.06206808292808
70-71	24.926013757874085	24.552939471560205	25.391316921519717	25.129729849045994
72-73	25.214176553973704	24.704900395385643	25.13163792157486	24.94928512906579
74-75	24.980517012583004	24.936848247682835	25.0505150034999	25.03211973623426
76-77	25.043554480689195	24.677300166028466	25.03610079431344	25.243044558968897
78-79	24.989241394315297	24.733914484682785	25.08286126118055	25.193982859821368
80-81	24.963168309229438	24.832573771357183	25.01586042188882	25.188397497524555
82-83	25.078220091071508	24.790258835647442	24.953464603829353	25.178056469451697
84-85	24.85642143974275	24.874143830960378	24.9227684422434	25.34666628705347
86-87	24.752136981805304	24.870946164469146	25.00965284622208	25.367264007503472
88-89	24.875300690817056	24.69582198527763	24.97530968074865	25.45356764315666
90-91	24.766517030701234	24.75279355834923	25.030749855007834	25.449939555941704
92-93	24.83072851274922	24.807793253835097	25.003814546200154	25.357663687215528
94-95	24.752085691775363	24.66549441635017	25.037961653531898	25.544458238342564
96-97	24.768797285285263	24.66394721800274	25.02519696171501	25.542058534996986
98-99	24.730232510532897	24.64665384679356	24.950550090263675	25.672563552409866
100-101	24.687481006381855	24.752387197901506	24.997840725516227	25.56229107020041
102-103	24.743437685520686	24.577908452578352	25.173173964666624	25.505479897234334
104-105	24.62245303333007	24.73766667114471	24.949893902377394	25.68998639314783
106-107	24.653311753943782	24.713859853379564	24.981352592725273	25.651475799951385
108-109	24.6761955371051	24.60865929196037	25.00479088238296	25.710354288551567
110-111	24.655613811527004	24.633192086058482	25.013209946674035	25.69798415574047
112-113	24.63993054678096	24.649687009477844	25.04547951094851	25.66490293279269
114-115	24.432422646414775	24.83119335661495	25.034752897780738	25.701631099189537
116-117	24.38511722829729	24.867337456596182	24.977696314813837	25.769849000292687
118-119	24.426047541209098	24.53551195887567	25.10237897976092	25.936061520154315
120-121	24.170132677054408	24.631538812652476	25.19888443671507	25.999444073578044
122-123	24.19354580763445	24.630145600433465	25.1464208572517	26.029887734680386
124-125	24.169323957657813	24.7175571884107	25.097250931558186	26.0158679223733
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	28.0
1	31.0
2	44.5
3	62.0
4	60.0
5	61.5
6	113.0
7	216.0
8	359.5
9	448.5
10	407.5
11	379.0
12	413.0
13	493.5
14	605.5
15	756.5
16	943.5
17	1063.0
18	1241.0
19	1458.5
20	1609.0
21	1783.5
22	1997.5
23	2293.0
24	2548.5
25	2910.0
26	3607.0
27	4290.5
28	4902.5
29	5892.5
30	7343.0
31	8751.5
32	10243.5
33	12030.0
34	13790.0
35	15777.0
36	18406.0
37	21238.0
38	23709.5
39	26584.0
40	29634.0
41	32517.0
42	35662.5
43	40132.0
44	44858.5
45	48146.5
46	52417.0
47	56198.5
48	60322.0
49	65299.5
50	66312.5
51	66448.0
52	67019.0
53	67579.5
54	68794.5
55	67962.0
56	64473.5
57	59565.0
58	56156.5
59	52623.5
60	47341.5
61	42300.0
62	36487.0
63	28889.5
64	22058.0
65	17526.5
66	13764.5
67	10520.5
68	8429.0
69	6974.0
70	5931.5
71	4968.0
72	4098.0
73	3634.0
74	2997.5
75	2332.5
76	1624.0
77	991.5
78	595.5
79	353.5
80	207.5
81	97.5
82	47.0
83	20.5
84	13.0
85	7.0
86	8.0
87	4.0
88	0.5
89	0.5
90	0.5
91	1.0
92	0.5
93	0.5
94	0.5
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12275903980553023
2	0.0015992579443138385
3	0.022325640902621183
4	0.05654976091093733
5	0.028722672679876538
6	0.022773433127029057
7	0.01791168897631499
8	0.006205120823937693
9	0.004989684786259176
10-11	0.008412096787090791
12-13	0.004989684786259176
14-15	0.0036463081130355514
16-17	0.012026419741240064
18-19	0.006141150506165139
20-21	0.015065009835436358
22-23	0.010075325049177181
24-25	0.015896623966479555
26-27	0.011002894656879208
28-29	0.016792208415295305
30-31	0.025012394249068433
32-33	0.015608757536503062
34-35	0.025076364566840984
36-37	0.017719778022997328
38-39	0.023828943370276193
40-41	0.011642597834604742
42-43	0.010810983703561547
44-45	0.019510946920628828
46-47	0.01871131794847191
48-49	0.024436661389115454
50-51	0.017975659294087544
52-53	0.025012394249068433
54-55	0.020310575892785745
56-57	0.024852468454637053
58-59	0.019351021126197444
60-61	0.023157255033664382
62-63	0.024692542660205662
64-65	0.01970285787394649
66-67	0.012506197124534216
68-69	0.02718738505333525
70-71	0.016984119368612965
72-73	0.02050248684610341
74-75	0.020662412640534793
76-77	0.01535287626541285
78-79	0.03342449103615922
80-81	0.023828943370276193
82-83	0.010715028226902717
84-85	0.014745158246573591
86-87	0.013721633162212733
88-89	0.023381151145868317
90-91	0.013401781573349966
92-93	0.008284156151545683
94-95	0.00860400774040845
96-97	0.002590797869788418
98-99	0.0017271985798589455
100-101	0.012794063554510708
102-103	0.0036463081130355514
104-105	0.0026867533464472486
106-107	3.198515888627677E-4
108-109	0.02306129955700555
110-111	5.757328599529818E-4
112-113	0.010139295366949736
114-115	0.0029426346175374625
116-117	0.00911577028258888
118-119	0.010235250843608566
120-121	0.004381966767419918
122-123	5.43747701066705E-4
124-125	0.02472452781909194
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1563225.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.439473872920836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.05911517407476	46.20173095631582
2	11.068587080786061	12.936848110354923
3	3.711776436098308	6.507427861784709
4	1.8141481444267553	4.240714523513407
5	1.0683977316747002	3.121830066304577
6	0.6817719905808318	2.3905437858502654
7	0.4675288688142843	1.912549877973602
8	0.3592820825627524	1.6797004701547644
9	0.27739608259071835	1.458979300890979
>10	1.4074576732846535	14.322069664542116
>50	0.06379476050425052	2.5410792984819763
>100	0.020085478902389032	2.1548952889916477
>500	3.292478497400772E-4	0.1329558013880702
>1k	3.292478497400772E-4	0.39867499345308455
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2662	0.1702889859105375	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2368	0.15148171248540676	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	6.397031777255354E-5
10-11	0.0	0.0	0.0	0.0	6.397031777255354E-5
12-13	0.0	0.0	0.0	0.0	1.919109533176606E-4
14-15	0.0	0.0	0.0	0.0	4.15807065521598E-4
16-17	0.0	0.0	0.0	0.0	7.036734954980889E-4
18-19	0.0	0.0	0.0	0.0	7.676438132706424E-4
20-21	0.0	0.0	0.0	3.198515888627677E-5	7.676438132706424E-4
22-23	0.0	0.0	0.0	1.2794063554510707E-4	9.275696077020262E-4
24-25	0.0	0.0	0.0	1.2794063554510707E-4	0.00108749540213341
26-27	0.0	0.0	0.0	3.5183674774904444E-4	0.0011834508787922403
28-29	0.0	0.0	0.0	0.0010555102432471333	0.001215436037678517
30-31	0.0	0.0	0.0	0.004413951926306194	0.0012474211965647938
32-33	0.0	0.0	0.0	0.010555102432471333	0.0012794063554510708
34-35	0.0	0.0	0.0	0.018231540565177756	0.0012794063554510708
36-37	0.0	0.0	0.0	0.029522301652033456	0.0012794063554510708
38-39	0.0	0.0	0.0	0.0465384061795327	0.0012794063554510708
40-41	0.0	0.0	0.0	0.06700890786674983	0.0013433766732236243
42-43	0.0	0.0	0.0	0.08709558764733163	0.0014393321498824545
44-45	0.0	0.0	0.0	0.11242783348526283	0.0015352876265412848
46-47	0.0	0.0	0.0	0.1396152185385981	0.001663228262086392
48-49	0.0	0.0	0.0	0.16942538662060802	0.0017271985798589455
50-51	0.0	0.0	0.0	0.19968334692702588	0.0017271985798589455
52-53	0.0	0.0	0.0	0.23122071358889473	0.0019191095331766062
54-55	0.0	0.0	0.0	0.2651569671672344	0.0019830798509491597
56-57	0.0	0.0	0.0	0.30472260870955875	0.0019830798509491597
58-59	0.0	0.0	0.0	0.3481904396360089	0.0019830798509491597
60-61	0.0	0.0	0.0	0.3928417214412513	0.0019830798509491597
62-63	0.0	0.0	0.0	0.443890035023749	0.002047050168721713
64-65	0.0	0.0	0.0	0.49413871963408973	0.002047050168721713
66-67	0.0	0.0	0.0	0.5541108925458587	0.0021110204864942667
68-69	0.0	0.0	0.0	0.6240304498712597	0.002206975963153097
70-71	0.0	0.0	0.0	0.7012746085816182	0.002334916598698204
72-73	0.0	0.0	0.0	0.7823250011994434	0.002782708823106079
74-75	0.0	0.0	0.0	0.865710310415967	0.002846679140878632
76-77	0.0	0.0	0.0	0.9598106478593933	0.002878664299764909
78-79	0.0	0.0	0.0	1.0633146220153848	0.002910649458651186
80-81	0.0	0.0	0.0	1.1777895056693692	0.003038590094196293
82-83	0.0	0.0	0.0	1.3025955956436213	0.003326456524172784
84-85	0.0	0.0	0.0	1.4299924834876618	0.003326456524172784
86-87	0.0	0.0	0.0	1.5675286666986519	0.0033904268419453374
88-89	0.0	0.0	0.0	1.7192342752962624	0.003454397159717891
90-91	0.0	0.0	0.0	1.8948647827408083	0.0035183674774904444
92-93	0.0	0.0	0.0	2.0864878696284923	0.003582337795262998
94-95	0.0	0.0	0.0	2.2811175614514863	0.003710278430808105
96-97	0.0	0.0	0.0	2.481120759967375	0.0037742487485806584
98-99	0.0	0.0	0.0	2.7010826976283004	0.003838219066353212
100-101	0.0	0.0	0.0	2.951686417502279	0.003838219066353212
102-103	0.0	0.0	0.0	3.221321306913592	0.0039021893841257654
104-105	0.0	0.0	0.0	3.51436933262966	0.003998144860784596
106-107	0.0	0.0	0.0	3.817300772441587	0.00415807065521598
108-109	0.0	0.0	0.0	4.128804234835037	0.004381966767419917
110-111	0.0	0.0	0.0	4.48726191047354	0.0045418925618513
112-113	0.0	0.0	0.0	4.8627676757984295	0.004605862879623854
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2200	0.0	81.56484	1
GTATCAA	4685	0.0	61.20609	1
TCAACGC	6165	0.0	46.07711	4
CAACGCA	6385	0.0	44.27034	5
ATCAACG	6470	0.0	43.905006	3
TATCAAC	6540	0.0	43.708252	2
AACGCAG	6650	0.0	42.58613	6
ACGCAGA	8000	0.0	35.46955	7
GTACATG	7945	0.0	34.21607	1
CGCAGAG	8565	0.0	33.059246	8
TACATGG	8245	0.0	32.575104	2
ACATGGG	8490	0.0	31.214203	3
GCAGAGT	9020	0.0	30.467367	9
CATGGGG	7400	0.0	29.21291	4
ACCGTAT	225	6.5701897E-9	26.438139	8
AGAGTAC	8475	0.0	26.42602	10-11
GTGGTAT	725	0.0	26.313047	1
CAGAGTA	8775	0.0	25.692036	10-11
TACCGTA	210	9.8358214E-8	25.494736	7
GAGTACT	4965	0.0	24.621016	12-13
>>END_MODULE