FastQCFastQC Report
Mon 15 Aug 2016
SRR2921544_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921544_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960927
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20050.077.4758151
GTATCAA39800.057.2737661
CAACGCA53350.042.265855
TCAACGC53000.042.20824
GTACATG107400.041.340391
AACGCAG55750.040.018446
TACATGG111150.039.932392
ATCAACG57250.039.4915353
ACATGGG115200.038.063693
TATCAAC63500.035.9793552
CATGGGG98600.034.8767284
ATGGGGG51200.032.0718465
ACGCAGA69200.032.0675247
CGCAGAG72950.030.0913438
GCAGAGT79550.027.0712879
AGAGTAC72450.025.41339910-11
CATGGGT18500.024.4414354
CAGAGTA76900.024.17487110-11
ATGGGGA37950.023.8295945
ATGGGTA15250.022.23775
Produced by FastQC (version 0.11.5)