FastQCFastQC Report
Mon 15 Aug 2016
SRR2921544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960927
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27980.14268761662213839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25670.13090747386312698No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27900.078.230991
GTATCAA54450.060.8946081
TCAACGC75450.043.409424
CAACGCA77150.042.421495
ATCAACG78500.041.8745353
AACGCAG79050.041.6202666
TATCAAC82150.040.4499782
GTACATG100700.037.2497251
TACATGG105650.034.890952
ACGCAGA95000.034.629797
ACATGGG106950.033.4080433
CGCAGAG100500.032.6737678
CATGGGG91300.031.3729134
GCAGAGT109150.029.6476549
ATGGGGG48450.028.7396325
AGAGTAC101550.027.06376810-11
CAGAGTA105400.025.53901710-11
GAGTACT55500.025.45703912-13
GTGGTAT12050.024.2499561
AGTACTT60600.023.46186612-13
Produced by FastQC (version 0.11.5)