##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921544_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1960927
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.384957726626233	33.0	31.0	34.0	28.0	34.0
2	31.449611841746275	33.0	31.0	34.0	28.0	34.0
3	31.528020675935412	33.0	31.0	34.0	28.0	34.0
4	35.037318064364456	37.0	35.0	37.0	32.0	37.0
5	34.70526337798398	37.0	35.0	37.0	32.0	37.0
6	34.6834655242138	37.0	35.0	37.0	31.0	37.0
7	34.5372826219436	37.0	35.0	37.0	31.0	37.0
8	34.51683107020302	37.0	35.0	37.0	31.0	37.0
9	36.115760556104334	39.0	37.0	39.0	31.0	39.0
10-11	35.979510965987004	39.0	37.0	39.0	30.0	39.0
12-13	35.887725805193156	39.0	37.0	39.0	30.0	39.0
14-15	37.07537047529051	40.0	37.0	41.0	31.0	41.0
16-17	37.06082811853781	40.0	37.0	41.0	31.0	41.0
18-19	37.07783130121621	40.0	37.0	41.0	30.0	41.0
20-21	37.05873370094858	40.0	37.0	41.0	30.0	41.0
22-23	36.99205044348923	40.0	37.0	41.0	30.0	41.0
24-25	36.91861655227349	40.0	37.0	41.0	30.0	41.0
26-27	36.79039785774789	40.0	36.5	41.0	30.0	41.0
28-29	36.59973471730462	40.0	36.0	41.0	30.0	41.0
30-31	36.556240492379374	40.0	36.0	41.0	30.0	41.0
32-33	36.4314625174726	40.0	36.0	41.0	30.0	41.0
34-35	36.30555064007992	40.0	35.5	41.0	29.0	41.0
36-37	36.17249545750556	40.0	35.0	41.0	29.0	41.0
38-39	36.0003008781051	40.0	35.0	41.0	28.0	41.0
40-41	35.79772806432876	40.0	35.0	41.0	27.0	41.0
42-43	35.62552354065195	39.5	35.0	41.0	27.0	41.0
44-45	35.410211853883396	39.0	35.0	41.0	26.0	41.0
46-47	35.1338899918253	39.0	35.0	41.0	24.5	41.0
48-49	34.88196883412794	39.0	35.0	41.0	24.0	41.0
50-51	34.662842624942186	39.0	34.0	41.0	23.0	41.0
52-53	34.464537180629364	38.5	34.0	41.0	23.0	41.0
54-55	34.24885041615522	38.0	34.0	40.5	23.0	41.0
56-57	33.98648470850776	38.0	33.5	40.0	22.0	41.0
58-59	33.843456181693654	37.0	33.5	40.0	22.0	41.0
60-61	33.72647961907812	37.0	33.5	40.0	22.0	41.0
62-63	33.45768276942487	36.5	33.0	40.0	22.0	41.0
64-65	33.16655999942884	36.0	33.0	39.5	21.0	41.0
66-67	32.861571593435144	35.5	33.0	39.0	20.0	41.0
68-69	32.54728375916085	35.0	33.0	39.0	20.0	41.0
70-71	32.246754213695866	35.0	33.0	37.5	20.0	40.0
72-73	31.934491697039206	35.0	32.0	37.0	18.5	39.5
74-75	31.645525815086437	35.0	32.0	36.5	18.0	39.0
76-77	31.351853740603296	35.0	32.0	36.0	17.5	39.0
78-79	31.077675252571872	35.0	32.0	36.0	16.5	37.0
80-81	30.857402391827947	35.0	32.0	35.0	14.5	37.0
82-83	30.671889111629348	35.0	32.0	35.0	13.0	36.5
84-85	30.479654010577647	35.0	31.0	35.0	11.0	36.0
86-87	30.336924322017087	35.0	31.0	35.0	10.0	36.0
88-89	30.237819408881617	35.0	31.0	35.0	9.0	36.0
90-91	30.13740440108173	35.0	31.0	35.0	7.0	35.0
92-93	30.201707661733458	35.0	31.5	35.0	7.0	35.0
94-95	30.16976843095128	35.0	32.0	35.0	7.0	35.0
96-97	30.15612207899631	35.0	32.0	35.0	7.0	35.0
98-99	30.11564912921287	35.0	32.0	35.0	6.0	35.0
100-101	30.065982568448494	35.0	32.0	35.0	2.0	35.0
102-103	29.18604466152998	33.5	29.5	35.0	2.0	35.0
104-105	29.891465363065528	34.5	31.0	35.0	2.0	35.0
106-107	30.000895239853396	35.0	32.0	35.0	2.0	35.0
108-109	30.00459808039769	35.0	32.0	35.0	2.0	35.0
110-111	29.971380117668836	35.0	32.0	35.0	2.0	35.0
112-113	29.928176061627994	35.0	32.0	35.0	2.0	35.0
114-115	29.86626733172627	35.0	32.0	35.0	2.0	35.0
116-117	29.790107433882035	35.0	32.0	35.0	2.0	35.0
118-119	29.695383356953116	35.0	32.0	35.0	2.0	35.0
120-121	29.577951652458253	35.0	31.0	35.0	2.0	35.0
122-123	29.45493279454054	35.0	31.5	35.0	2.0	35.0
124-125	28.53372053115695	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28925.0
3	11139.0
4	6373.0
5	5371.0
6	5753.0
7	6867.0
8	7961.0
9	8778.0
10	10183.0
11	11103.0
12	11756.0
13	13141.0
14	16894.0
15	14052.0
16	7671.0
17	6549.0
18	5886.0
19	5470.0
20	5230.0
21	5573.0
22	6656.0
23	8940.0
24	12644.0
25	17380.0
26	21764.0
27	24409.0
28	28539.0
29	34315.0
30	39862.0
31	48134.0
32	59914.0
33	77406.0
34	109068.0
35	174356.0
36	348079.0
37	568682.0
38	185795.0
39	309.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.56594775646917	17.562649347466454	12.72726344382493	25.144139452239443
2	16.780968272634826	19.80764896011717	38.08089575469148	25.330487012556517
3	18.675797596143056	24.59765694337613	29.25469621262974	27.471849247851075
4	13.390321385674033	15.850590817756272	35.47121019927279	35.2878775972969
5	14.40889526395955	37.18393155731391	33.260015486524786	15.147157692201748
6	33.20163344585462	35.00569747383089	17.088646980032827	14.70402210028166
7	29.376160359073754	31.67351831072121	20.650872181985104	18.29944914821993
8	27.735048946483985	33.28457809635279	19.397801424407707	19.582571532755512
9	28.158837400961218	14.119095411781025	18.84541790082658	38.876649286431174
10-11	26.352728355785302	24.63262031703742	26.948282773470535	22.06636855370674
12-13	26.868991881781067	22.514676771877717	27.20272699942091	23.413604346920305
14-15	24.337750497418625	23.466480627128675	24.859686257170594	27.33608261828211
16-17	24.04470926887784	26.40114917438155	25.315421132731373	24.238720424009237
18-19	24.111618238533598	25.324015153813967	27.311926883893094	23.252439723759345
20-21	25.353378787786053	24.278629878754124	26.980522726206285	23.387468607253535
22-23	25.13552232898992	23.913156567369477	26.797618458679267	24.153702644961335
24-25	24.49129845575068	24.46801511147698	26.82419774470336	24.21648868806898
26-27	24.201634128348005	24.513990767258985	27.005091701370837	24.279283403022177
28-29	24.274529805546702	24.665196047178835	26.91345871852088	24.146815428753587
30-31	24.64029662282428	24.434325553139185	26.77050063772989	24.15487718630665
32-33	24.316094772429423	24.49511854204266	26.735312846587593	24.453473838940322
34-35	24.469015195915656	24.631043412748326	26.82937839620375	24.070562995132267
36-37	24.63726794814055	24.5336775804636	26.616374418738687	24.212680052657163
38-39	24.643833118463856	24.662578408231607	26.504411519044325	24.189176954260216
40-41	24.66461963108988	24.572791189365752	26.517891500654223	24.24469767889014
42-43	24.589955330446823	24.614690996701995	26.33805291545528	24.4573007573959
44-45	24.55687118399674	24.67079181597444	26.198812542541717	24.5735244574871
46-47	24.689742549370994	24.766989724264054	25.943141080782077	24.600126645582872
48-49	24.842498940301347	24.97052992073811	25.50611768750545	24.68085345145509
50-51	24.744808049442433	25.214617284756624	25.375258277767376	24.66531638803356
52-53	24.953059893077043	25.055509449996098	25.45334630296606	24.538084353960794
54-55	24.769395361766644	24.944346045311207	25.603700997550437	24.682557595371712
56-57	24.660495173212688	24.94961018186752	25.4388817349757	24.951012909944094
58-59	24.702535790575403	24.852823563735484	25.5901594024601	24.854481243229017
60-61	24.835124926071135	24.933620233006945	25.645683663780584	24.585571177141336
62-63	24.704373700717156	24.993821592062872	25.584576857481917	24.71722784973806
64-65	24.71000002295262	24.781357168013944	25.652358088414	24.856284720619435
66-67	24.667929995177726	25.01415953079867	25.597754059979287	24.720156414044318
68-69	24.787071841861085	24.93627585756602	25.417452431974446	24.85919986859845
70-71	24.659710324700132	24.779545539166655	25.751003897258457	24.80974023887476
72-73	24.874812266217404	25.0398675273411	25.37964367751895	24.70567652892254
74-75	24.683645025464987	25.1607851938374	25.29424366308176	24.861326117615857
76-77	24.634727778029962	24.857892312847554	25.43234143995316	25.075038469169325
78-79	24.617845385360756	24.87502547442368	25.507173776252046	24.999955363963515
80-81	24.620908004900855	25.041520571045574	25.301483212733245	25.036088211320322
82-83	24.716376576730497	25.069304102909996	25.26698431844059	24.947335001918916
84-85	24.55441742042936	25.047082275974436	25.26374362556349	25.134756678032716
86-87	24.358500617521493	25.14899953357555	25.302340716304535	25.190159132598428
88-89	24.51898909476529	24.925037509936978	25.320119225873455	25.235854169424282
90-91	24.457144168646217	24.869834202295387	25.38914581604016	25.283875813018238
92-93	24.490912206580912	25.078030887991503	25.273618113774134	25.157438791653448
94-95	24.385765205865237	24.861342949948824	25.409876614988068	25.343015229197874
96-97	24.378980221764092	24.952323397679855	25.288577632093954	25.380118748462095
98-99	24.273068095673636	24.88717527272616	25.277022666429698	25.5627339651705
100-101	24.260841513903888	24.932211450801184	25.41867222932714	25.388274805967793
102-103	24.388187181803023	24.73803492663652	25.530190177438083	25.34358771412237
104-105	24.179074564169227	24.87853039564094	25.41428434099664	25.528110699193196
106-107	24.213507727487375	24.88592101062663	25.303352198608614	25.597219063277375
108-109	24.26299320918055	24.786707774923368	25.34656081350074	25.60373820239534
110-111	24.161532795030073	24.956238530542766	25.31122596115231	25.571002713274854
112-113	24.17588358341177	24.904022768821036	25.374097635523928	25.545996012243265
114-115	23.91564712095045	25.062707766889535	25.355764741520588	25.665880370639428
116-117	23.9458230343905	25.003499919291478	25.35382335263453	25.69685369368349
118-119	23.912683140538608	24.693186843003158	25.477337163217	25.916792853241233
120-121	23.725547944070243	24.80186551512671	25.55306939201189	25.91951714879115
122-123	23.722687481449977	24.90741562353066	25.453564001276956	25.916332893742407
124-125	23.6472585052838	24.870586044945796	25.406528273158642	26.07562717661176
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	32.5
2	69.5
3	88.0
4	65.0
5	73.0
6	123.5
7	247.0
8	433.5
9	522.5
10	430.5
11	366.0
12	434.5
13	508.5
14	587.5
15	707.5
16	855.5
17	1023.5
18	1179.5
19	1332.5
20	1531.5
21	1788.5
22	2031.0
23	2379.0
24	2835.0
25	3471.0
26	4563.0
27	5868.0
28	6933.0
29	8559.5
30	10836.0
31	12886.0
32	15490.5
33	18398.0
34	21536.0
35	24937.0
36	28641.5
37	32314.5
38	35312.5
39	38812.5
40	42213.5
41	44878.0
42	47836.0
43	53896.5
44	59855.5
45	63858.5
46	68161.0
47	72061.5
48	77939.0
49	83024.0
50	82666.0
51	81241.5
52	80754.5
53	80858.0
54	80965.0
55	79709.0
56	76008.5
57	70040.5
58	66056.5
59	61429.0
60	54212.0
61	47637.0
62	41250.0
63	32838.0
64	25239.5
65	20246.5
66	15793.5
67	12180.0
68	9818.5
69	7987.0
70	6860.0
71	5841.0
72	4780.5
73	4317.5
74	3474.0
75	2564.0
76	1801.0
77	1034.0
78	587.0
79	377.5
80	210.5
81	82.5
82	54.5
83	31.0
84	16.0
85	10.5
86	5.0
87	2.0
88	1.0
89	1.0
90	0.5
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12274806762311906
2	0.0016828775370016323
3	0.02095947477902033
4	0.056146914189054466
5	0.026161096257025375
6	0.020857482201020232
7	0.016675786503016175
8	0.0057115843680055406
9	0.004028706831003908
10-11	0.0075984470610073705
12-13	0.004615164154504477
14-15	0.0027283014615026462
16-17	0.010964202135010636
18-19	0.005125127044504971
20-21	0.014431949787013999
22-23	0.009179332020008904
24-25	0.015196894122014741
26-27	0.009561804187509276
28-29	0.015400879278014938
30-31	0.02333080221752263
32-33	0.014967410821514518
34-35	0.02376427067402305
36-37	0.016318812480015828
38-39	0.022157887570521492
40-41	0.010479737389510165
42-43	0.00996977449950967
44-45	0.01792519558351739
46-47	0.017211247537516693
48-49	0.023662278096022952
50-51	0.01754272341601702
52-53	0.02294833005002226
54-55	0.019098110230518526
56-57	0.02353478737352283
58-59	0.018103682595017562
60-61	0.021800913547521148
62-63	0.023993753974523274
64-65	0.01869013991851813
66-67	0.01190763348151155
68-69	0.02672205543602592
70-71	0.01621681990201573
72-73	0.018945121363518377
74-75	0.01999054528801939
76-77	0.013565012874013158
78-79	0.03200017134753104
80-81	0.02310131891702241
82-83	0.009408815320509127
84-85	0.013437522151513035
86-87	0.013590511018513182
88-89	0.022769843038522086
90-91	0.012978555550512588
92-93	0.008414387685008162
94-95	0.007853428506007617
96-97	0.0030342791955029432
98-99	0.001606383103501558
100-101	0.0119586297705116
102-103	0.0037737253860036604
104-105	0.0023203311495022506
106-107	2.549814450002473E-4
108-109	0.021035969212520406
110-111	6.374536125006183E-4
112-113	0.00894984871950868
114-115	0.0023203311495022506
116-117	0.008541878407508285
118-119	0.01050523553401019
120-121	0.0037992235305036853
122-123	6.62951757000643E-4
124-125	0.023177813350522485
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1960927.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.820833339337675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.9542677753476	44.86247290692465
2	11.010972153424	12.513052272675862
3	3.6771908334193055	6.268231425079756
4	1.818255700027493	4.132592163982518
5	1.077518301804161	3.061274392345019
6	0.7008211514940792	2.3892745109836673
7	0.48493233885913634	1.9287981725019145
8	0.3937861477225029	1.7900205656864117
9	0.28264894852803335	1.4454313918065373
>10	1.4939068595429432	15.075253492559035
>50	0.07444827821215137	2.8450600350211204
>100	0.030351288422381335	3.0842868018392506
>500	5.401339177393888E-4	0.19309665655999136
>1k	3.600892784929254E-4	0.41115521203422756
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2798	0.14268761662213839	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2567	0.13090747386312698	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.099628900004947E-5
2	0.0	0.0	0.0	0.0	5.099628900004947E-5
3	0.0	0.0	0.0	0.0	5.099628900004947E-5
4	0.0	0.0	0.0	0.0	5.099628900004947E-5
5	0.0	0.0	0.0	0.0	5.099628900004947E-5
6	0.0	0.0	0.0	0.0	5.099628900004947E-5
7	0.0	0.0	0.0	0.0	5.099628900004947E-5
8	0.0	0.0	0.0	0.0	5.099628900004947E-5
9	0.0	0.0	0.0	0.0	5.099628900004947E-5
10-11	0.0	0.0	0.0	0.0	5.099628900004947E-5
12-13	0.0	0.0	0.0	0.0	1.529888670001484E-4
14-15	0.0	0.0	0.0	0.0	2.0398515600019785E-4
16-17	0.0	0.0	0.0	2.5498144500024734E-5	2.549814450002473E-4
18-19	0.0	0.0	0.0	5.099628900004947E-5	2.549814450002473E-4
20-21	0.0	0.0	0.0	5.099628900004947E-5	2.549814450002473E-4
22-23	0.0	0.0	0.0	5.099628900004947E-5	2.804795895002721E-4
24-25	0.0	0.0	0.0	1.0199257800009894E-4	3.059777340002968E-4
26-27	0.0	0.0	0.0	1.2749072250012368E-4	3.059777340002968E-4
28-29	0.0	0.0	0.0	7.904424795007667E-4	3.569740230003463E-4
30-31	0.0	0.0	0.0	0.003722729097003611	3.569740230003463E-4
32-33	0.0	0.0	0.0	0.010913205846010585	3.569740230003463E-4
34-35	5.099628900004947E-5	0.0	0.0	0.020475010033519862	3.569740230003463E-4
36-37	5.099628900004947E-5	0.0	0.0	0.03508544683203403	3.569740230003463E-4
38-39	5.099628900004947E-5	0.0	0.0	0.057447319558555726	3.569740230003463E-4
40-41	5.099628900004947E-5	0.0	0.0	0.083506423237581	3.569740230003463E-4
42-43	1.0199257800009894E-4	0.0	0.0	0.10913205846010585	4.0797031200039575E-4
44-45	1.0199257800009894E-4	0.0	0.0	0.14049477619513628	4.0797031200039575E-4
46-47	1.0199257800009894E-4	0.0	0.0	0.17425431951316903	4.0797031200039575E-4
48-49	1.0199257800009894E-4	0.0	0.0	0.21104814202670472	4.0797031200039575E-4
50-51	1.0199257800009894E-4	0.0	0.0	0.2503407827012428	4.0797031200039575E-4
52-53	2.0398515600019787E-4	0.0	0.0	0.2919792526697832	4.0797031200039575E-4
54-55	2.549814450002473E-4	0.0	0.0	0.33815639235932804	4.334684565004205E-4
56-57	2.549814450002473E-4	0.0	0.0	0.38966264424937797	4.589666010004452E-4
58-59	2.549814450002473E-4	0.0	0.0	0.44644701205093307	4.589666010004452E-4
60-61	2.549814450002473E-4	0.0	0.0	0.5114672805259961	4.8446474550046994E-4
62-63	2.549814450002473E-4	0.0	0.0	0.5757481028105584	5.609591790005441E-4
64-65	2.549814450002473E-4	0.0	0.0	0.6459954909081267	5.609591790005441E-4
66-67	3.059777340002968E-4	0.0	0.0	0.7224899244082008	5.609591790005441E-4
68-69	3.569740230003463E-4	0.0	0.0	0.8101270470547859	5.609591790005441E-4
70-71	3.569740230003463E-4	0.0	0.0	0.9085243866803813	6.119554680005936E-4
72-73	3.569740230003463E-4	0.0	0.0	1.0075846780629774	6.374536125006184E-4
74-75	3.569740230003463E-4	0.0	0.0	1.1199550008745862	6.629517570006431E-4
76-77	4.8446474550046994E-4	0.0	0.0	1.241249674261204	6.629517570006431E-4
78-79	5.099628900004946E-4	0.0	0.0	1.3771547844463359	6.629517570006431E-4
80-81	5.099628900004946E-4	0.0	0.0	1.5230551672754773	7.139480460006926E-4
82-83	5.099628900004946E-4	0.0	0.0	1.6775994210901273	8.66936913000841E-4
84-85	5.099628900004946E-4	0.0	0.0	1.842291936415787	9.179332020008904E-4
86-87	5.099628900004946E-4	0.0	0.0	2.023022784631962	9.689294910009399E-4
88-89	5.099628900004946E-4	0.0	0.0	2.222367278333156	0.0010199257800009892
90-91	5.099628900004946E-4	0.0	0.0	2.446546964777373	0.0010199257800009892
92-93	5.099628900004946E-4	0.0	0.0	2.6875044303026066	0.0011219183580010882
94-95	5.099628900004946E-4	0.0	0.0	2.9344794579298465	0.0011219183580010882
96-97	5.099628900004946E-4	0.0	0.0	3.204122336017608	0.0011474165025011128
98-99	5.099628900004946E-4	0.0	0.0	3.4993653511833944	0.0011729146470011377
100-101	5.099628900004946E-4	0.0	0.0	3.830382263082716	0.0011729146470011377
102-103	5.099628900004946E-4	0.0	0.0	4.1881977248515625	0.0012749072250012369
104-105	5.099628900004946E-4	0.0	0.0	4.560878604863924	0.0013259035140012862
106-107	5.099628900004946E-4	0.0	0.0	4.945951583103298	0.0013259035140012862
108-109	5.099628900004946E-4	0.0	0.0	5.351933039832692	0.001427896092001385
110-111	5.099628900004946E-4	0.0	0.0	5.800470899732627	0.0014788923810014345
112-113	5.099628900004946E-4	0.0	0.0	6.277566681472589	0.0014788923810014345
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2790	0.0	78.23099	1
GTATCAA	5445	0.0	60.894608	1
TCAACGC	7545	0.0	43.40942	4
CAACGCA	7715	0.0	42.42149	5
ATCAACG	7850	0.0	41.874535	3
AACGCAG	7905	0.0	41.620266	6
TATCAAC	8215	0.0	40.449978	2
GTACATG	10070	0.0	37.249725	1
TACATGG	10565	0.0	34.89095	2
ACGCAGA	9500	0.0	34.62979	7
ACATGGG	10695	0.0	33.408043	3
CGCAGAG	10050	0.0	32.673767	8
CATGGGG	9130	0.0	31.372913	4
GCAGAGT	10915	0.0	29.647654	9
ATGGGGG	4845	0.0	28.739632	5
AGAGTAC	10155	0.0	27.063768	10-11
CAGAGTA	10540	0.0	25.539017	10-11
GAGTACT	5550	0.0	25.457039	12-13
GTGGTAT	1205	0.0	24.249956	1
AGTACTT	6060	0.0	23.461866	12-13
>>END_MODULE