FastQCFastQC Report
Mon 15 Aug 2016
SRR2921543_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921543_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504786
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25400.064.66191
GTATCAA51350.060.6086161
TCAACGC68450.044.5901534
CAACGCA69100.044.0846065
AACGCAG73200.041.776276
TATCAAC75450.040.8442422
ATCAACG75300.040.6095733
GTACATG138550.035.476981
ACGCAGA86250.035.314567
ACATGGG141550.034.463893
TACATGG146000.033.5764432
CGCAGAG93550.032.5575568
CATGGGG114200.032.3013424
GCAGAGT101450.029.0846549
ATGGGGG59500.027.898595
AGAGTAC93300.026.84295810-11
CAGAGTA100400.022.98941810-11
ATGGGGA48550.022.6713565
GTATTAC6600.022.5407921
GTATAGG7300.022.0096951
Produced by FastQC (version 0.11.5)