##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921543_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2504786 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.137855289833144 31.0 31.0 34.0 26.0 34.0 2 30.232593522959647 31.0 31.0 34.0 26.0 34.0 3 30.31167532875064 31.0 31.0 34.0 26.0 34.0 4 33.717019737414695 37.0 35.0 37.0 28.0 37.0 5 33.74695962050251 37.0 35.0 37.0 28.0 37.0 6 33.71495049876516 37.0 35.0 37.0 28.0 37.0 7 33.61778571103479 37.0 35.0 37.0 28.0 37.0 8 33.56362739172129 37.0 35.0 37.0 27.0 37.0 9 35.067255246555995 39.0 35.0 39.0 27.0 39.0 10-11 34.96353800284735 39.0 35.0 39.0 27.0 39.0 12-13 34.83479965953179 39.0 35.0 39.0 27.0 39.0 14-15 36.00906564472973 40.0 36.0 41.0 27.0 41.0 16-17 35.93078929696988 40.0 36.0 41.0 27.0 41.0 18-19 35.94254838537104 40.0 36.0 41.0 27.0 41.0 20-21 35.964658857084 40.0 36.0 41.0 27.0 41.0 22-23 35.91996941056043 40.0 36.0 41.0 27.0 41.0 24-25 35.86444710246704 40.0 36.0 41.0 27.0 41.0 26-27 35.78205183995759 40.0 36.0 41.0 26.0 41.0 28-29 35.73794687450345 40.0 36.0 41.0 26.0 41.0 30-31 35.64356475962418 40.0 35.0 41.0 26.0 41.0 32-33 35.54691179206527 40.0 35.0 41.0 25.0 41.0 34-35 35.38025144663057 40.0 35.0 41.0 25.0 41.0 36-37 35.251889782200955 40.0 35.0 41.0 25.0 41.0 38-39 35.13068741201843 40.0 35.0 41.0 24.0 41.0 40-41 34.994521488063256 39.0 35.0 41.0 24.0 41.0 42-43 34.80827503826674 39.0 34.5 41.0 23.0 41.0 44-45 34.629076895191844 39.0 34.0 41.0 23.0 41.0 46-47 34.40490225512279 39.0 34.0 41.0 22.0 41.0 48-49 34.27153956465742 39.0 34.0 41.0 20.5 41.0 50-51 34.090163990057434 38.5 33.0 41.0 20.0 41.0 52-53 33.903500937804665 38.0 33.0 40.0 19.0 41.0 54-55 33.70854156802218 38.0 33.0 40.0 18.5 41.0 56-57 33.47344723261788 37.5 33.0 40.0 17.5 41.0 58-59 33.239038584533766 37.0 33.0 40.0 16.5 41.0 60-61 32.93420855114968 37.0 32.5 40.0 13.5 41.0 62-63 32.6194299632783 36.0 32.0 39.5 13.0 41.0 64-65 32.31408571430853 35.5 32.0 39.0 11.0 41.0 66-67 32.2728342860428 35.0 32.0 39.0 11.0 41.0 68-69 32.02533270307324 35.0 32.0 39.0 8.0 40.5 70-71 31.726576442059322 35.0 32.0 37.5 7.5 40.0 72-73 31.389462413156252 35.0 32.0 37.0 7.0 39.5 74-75 31.083547855984502 35.0 31.0 36.5 3.5 39.0 76-77 30.79667284949692 35.0 31.0 36.0 2.0 39.0 78-79 30.515813127349006 35.0 31.0 36.0 2.0 37.5 80-81 30.270263407732237 35.0 31.0 35.0 2.0 37.0 82-83 30.068874945803753 35.0 31.0 35.0 2.0 36.5 84-85 29.86807994774803 35.0 31.0 35.0 2.0 36.0 86-87 29.717992075969764 35.0 31.0 35.0 2.0 36.0 88-89 29.587139979223775 35.0 30.0 35.0 2.0 36.0 90-91 29.47808156066027 35.0 30.0 35.0 2.0 35.0 92-93 29.383875708343947 34.0 30.0 35.0 2.0 35.0 94-95 29.327632580188485 34.0 30.0 35.0 2.0 35.0 96-97 29.230467592840267 34.0 30.0 35.0 2.0 35.0 98-99 29.172095739915505 34.0 30.0 35.0 2.0 35.0 100-101 29.11527891005459 34.0 30.0 35.0 2.0 35.0 102-103 28.13571997767474 33.5 28.0 34.5 2.0 35.0 104-105 28.96910774014227 34.0 29.5 35.0 2.0 35.0 106-107 29.299609028475885 35.0 31.0 35.0 2.0 35.0 108-109 29.343263456438994 35.0 31.0 35.0 2.0 35.0 110-111 29.286968627259974 35.0 31.0 35.0 2.0 35.0 112-113 29.226968691137685 35.0 31.0 35.0 2.0 35.0 114-115 29.11164147356301 35.0 31.0 35.0 2.0 35.0 116-117 29.057265371173425 35.0 31.0 35.0 2.0 35.0 118-119 28.907116416332574 35.0 30.5 35.0 2.0 35.0 120-121 28.7469077198611 35.0 30.0 35.0 2.0 35.0 122-123 28.633875508726092 35.0 30.0 35.0 2.0 35.0 124-125 27.780968713494886 34.0 27.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84681.0 3 15396.0 4 10189.0 5 8816.0 6 9578.0 7 10756.0 8 12039.0 9 12457.0 10 13062.0 11 13545.0 12 13627.0 13 14034.0 14 15324.0 15 13300.0 16 10025.0 17 8884.0 18 7923.0 19 7711.0 20 7683.0 21 7876.0 22 9542.0 23 12639.0 24 17845.0 25 24162.0 26 29086.0 27 32095.0 28 37777.0 29 45228.0 30 52091.0 31 62912.0 32 79187.0 33 102909.0 34 145850.0 35 229526.0 36 440066.0 37 683663.0 38 203161.0 39 141.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.73444479722673 17.619877750953012 12.918301612880681 24.727375838939576 2 16.358269846917768 19.706407026295896 38.6820863079799 25.253236818806435 3 18.256202716863466 25.030550337371572 29.332556995348362 27.380689950416603 4 13.299570718779465 15.97070711790517 35.93961228505487 34.790109878260495 5 14.414644686005579 37.24896141935494 33.57313277138286 14.763261123256619 6 32.78994573846219 34.975545361407406 17.953798806925185 14.280710093205226 7 29.302044334247412 31.263264848042855 21.43463650022885 18.00005431748088 8 27.811838154038103 32.87332854877237 20.053980071784387 19.26085322540514 9 27.920685873663846 14.370383918965437 19.18316881108682 38.52576139628389 10-11 25.976342373414763 24.8510315565485 27.470400010224104 21.702226059812627 12-13 26.4605838261653 22.78387361136405 27.32799205717809 23.42755050529255 14-15 24.15112748353694 23.919871849913278 24.66459845866852 27.26440220788126 16-17 24.120505678781985 26.672223371548657 25.16889773559617 24.038373214073186 18-19 24.185668533100916 26.049359982680425 26.453669744192794 23.311301740025865 20-21 25.60880076635393 24.96021223338002 25.861848565203598 23.56913843506245 22-23 25.41758881095864 24.6149762557623 25.68941374691201 24.278021186367052 24-25 24.69776227028055 25.037991947784445 25.77024579312086 24.493999988814146 26-27 24.33176124965175 25.179188581140387 25.891169736107628 24.597880433100233 28-29 24.365528360720006 25.148813119879915 26.009487691424948 24.47617082797513 30-31 24.803100699642588 25.014978646441634 25.740684080774447 24.441236573141328 32-33 24.51995767310744 25.099076326220665 25.678794170904617 24.702171829767277 34-35 24.73504125352566 25.167506328416245 25.784491965367646 24.312960452690447 36-37 24.828045976368717 24.883368447457734 25.68488520187809 24.60370037429546 38-39 24.7797183926803 25.15622546961518 25.637555312584766 24.426500825119753 40-41 24.78274154861928 25.02305693635327 25.83922654912855 24.3549749658989 42-43 24.652222405090193 25.05878184366067 25.626247341534697 24.662748409714442 44-45 24.64635921059938 25.04315274493802 25.605666401818066 24.70482164264454 46-47 24.72377025931119 25.016259383102224 25.590651054462942 24.669319303123636 48-49 24.72246326521442 25.268060333421538 25.436598481179505 24.572877920184535 50-51 24.732871170817255 25.379101149760906 25.24672755825247 24.64130012116937 52-53 24.797201598778244 25.156908727786455 25.409057253334765 24.636832420100536 54-55 24.790123397616405 25.089770228158176 25.48625706678426 24.633849307441164 56-57 24.658501592860407 25.218419185899748 25.313279854549638 24.809799366690203 58-59 24.77158357934073 25.043386638153642 25.41088993633697 24.774139846168655 60-61 24.8317785357459 25.176738122057984 25.4996322760763 24.491851066119814 62-63 24.603160021679766 25.286603877355144 25.48628698850549 24.623949112459595 64-65 24.746642755651322 25.023170452854792 25.471322258512558 24.758864532981324 66-67 24.671985718236705 25.19361354371244 25.38569447370444 24.748706264346414 68-69 24.69078166648729 25.139900292150745 25.39251454314081 24.776803498221156 70-71 24.63576822896383 24.95703113609956 25.554121595793504 24.853079039143108 72-73 24.754838973769154 25.198146578448434 25.41913145575061 24.627882992031804 74-75 24.635622541977344 25.30770339856641 25.305247123262287 24.751426936193955 76-77 24.63531073119165 25.08824947154152 25.38226128712638 24.894178510140453 78-79 24.61922497535915 25.13622356799517 25.405056446318092 24.839495010327592 80-81 24.617936670332558 25.29165398779474 25.26495847320026 24.825450868672444 82-83 24.753072037218836 25.167275713381276 25.35674803993973 24.722904209460157 84-85 24.56072502582561 25.20429885113467 25.241454987168055 24.993521135871664 86-87 24.4808983407075 25.299693473417555 25.38766039338343 24.831747792491505 88-89 24.586121226872155 25.00353904661578 25.43892663761021 24.97141308890185 90-91 24.4777515731589 25.152654160987574 25.41780373261759 24.951790533235933 92-93 24.58296022321718 25.308475963722216 25.319696720124984 24.788867092935615 94-95 24.576963013998647 25.03974472338012 25.368643165155376 25.014649097465863 96-97 24.522699141545218 25.089199441006187 25.413335995208623 24.974765422239965 98-99 24.410647435365064 25.164462126197034 25.27961356391304 25.145276874524857 100-101 24.476832747389203 25.143211932098357 25.475553053397775 24.90440226711466 102-103 24.51483091323562 24.945615426553328 25.55646742724884 24.98308623296221 104-105 24.387524527266816 25.155588064491464 25.436450098727924 25.02043730951379 106-107 24.41599426959381 25.149344119635803 25.380025709368347 25.05463590140204 108-109 24.39438484577572 25.0426742617203 25.396146918449137 25.166793974054848 110-111 24.369856103144347 25.221846226143708 25.44986607676695 24.958431593944997 112-113 24.33330425274637 25.138423926681785 25.55359089683133 24.974680923740518 114-115 24.223603352457186 25.39359350238825 25.435856566705965 24.946946578448593 116-117 24.191410365902104 25.397482289424524 25.409819702089415 25.001287642583957 118-119 24.47330028072109 25.008509276762503 25.392102070500634 25.126088372015776 120-121 24.216318461701196 25.181902103012682 25.526514435173937 25.075265000112186 122-123 24.229601966257416 25.238369175652288 25.45322141581636 25.078807442273938 124-125 24.163838304875377 25.28950700066954 25.520294136317922 25.026360558137156 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 55.0 1 76.0 2 111.5 3 122.5 4 123.5 5 121.0 6 126.5 7 164.0 8 215.5 9 254.5 10 279.0 11 312.5 12 362.5 13 416.0 14 477.5 15 572.5 16 672.0 17 749.5 18 863.0 19 1009.5 20 1131.0 21 1234.0 22 1443.5 23 1724.5 24 2085.0 25 2744.5 26 3982.5 27 5473.0 28 6795.0 29 9012.0 30 12364.5 31 15560.5 32 19584.5 33 24207.5 34 28920.0 35 33950.0 36 39777.0 37 46073.5 38 50399.0 39 54673.0 40 59419.5 41 63016.5 42 66690.5 43 73331.5 44 80593.5 45 85756.5 46 90283.5 47 94148.0 48 99076.5 49 103676.5 50 102853.5 51 101358.5 52 101533.5 53 100914.5 54 100954.0 55 99172.0 56 94108.5 57 86568.0 58 80619.5 59 75426.5 60 68074.0 61 60681.0 62 52261.5 63 41323.5 64 31519.0 65 24952.0 66 19715.0 67 15671.0 68 12644.0 69 10229.5 70 8607.0 71 6981.0 72 5593.5 73 4968.0 74 4037.5 75 3130.0 76 2343.5 77 1507.0 78 966.0 79 614.0 80 401.0 81 253.5 82 152.5 83 94.0 84 63.5 85 46.5 86 40.5 87 37.5 88 24.0 89 19.0 90 17.5 91 12.5 92 9.0 93 5.5 94 6.5 95 11.5 96 10.5 97 6.0 98 6.5 99 6.0 100 27.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03517266544926393 2 0.028225964214108515 3 0.028824817768863285 4 0.03325633407404864 5 0.03545213044148283 6 0.04675050084118963 7 0.03944448747318134 8 0.03589128971496966 9 0.030661302003444607 10-11 0.03593121328528665 12-13 0.03553197758211679 14-15 0.033815264058486436 16-17 0.03609090756655459 18-19 0.050303698599401314 20-21 0.06004504975674568 22-23 0.05335785172865067 24-25 0.06483587819478391 26-27 0.04824763472807657 28-29 0.048966258993782305 30-31 0.04878660292735587 32-33 0.05641200485790004 34-35 0.03992357031698516 36-37 0.05120197893153347 38-39 0.06040436188959855 40-41 0.04794820795069918 42-43 0.05836825980343231 44-45 0.0586477247956512 46-47 0.06451648963224803 48-49 0.047848399024906715 50-51 0.05052327823614472 52-53 0.05986539369031925 54-55 0.04679042441150661 56-57 0.044474857333121474 58-59 0.04531325230977816 60-61 0.0347135443906186 62-63 0.04267829666885714 64-65 0.042259099180528795 66-67 0.03567171007822624 68-69 0.030761110929237067 70-71 0.04906606791957477 72-73 0.0306812637886031 74-75 0.03972395246540024 76-77 0.03213847410517306 78-79 0.03249778623802592 80-81 0.0247526135965308 82-83 0.01846465127160564 84-85 0.019522625885005744 86-87 0.01738671487304704 88-89 0.023175632569009886 90-91 0.0139932113961033 92-93 0.019901899803017105 94-95 0.014592064950858078 96-97 0.011418141110657757 98-99 0.010300281141782172 100-101 0.01121852325907283 102-103 0.006767045168728985 104-105 0.007006586590630896 106-107 0.01004077793472177 108-109 0.013933326040627822 110-111 0.007166280871898836 112-113 0.01004077793472177 114-115 0.009382039024491513 116-117 0.0083440261962499 118-119 0.24123817364038286 120-121 0.3568368714932134 122-123 0.02952348024941053 124-125 0.0038725863207475612 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2504786.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.014936906790226 #Duplication Level Percentage of deduplicated Percentage of total 1 78.3865515071969 43.124311855093 2 11.15937013805012 12.278640881286925 3 3.8168365526588226 6.299490663841675 4 1.8597868759575562 4.092642305635259 5 1.09947161496268 3.02436807639893 6 0.7050828379587587 2.3274052706617026 7 0.5213774736117075 2.0078484170758846 8 0.3743973509731793 1.6477957313487082 9 0.2935872675796781 1.453651650227964 >10 1.6453586411564303 16.41915013287759 >50 0.09941132520799602 3.7199961431025192 >100 0.03832977633415684 3.391133670286882 >500 2.19319176011434E-4 0.07388310289538763 >1k 2.1931917601048545E-4 0.13968209926767763 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.197707109509555E-4 7 0.0 0.0 0.0 0.0 1.197707109509555E-4 8 0.0 0.0 0.0 0.0 1.197707109509555E-4 9 0.0 0.0 0.0 0.0 1.5969428126794065E-4 10-11 0.0 0.0 0.0 0.0 1.5969428126794065E-4 12-13 0.0 0.0 0.0 0.0 2.39541421901911E-4 14-15 0.0 0.0 0.0 0.0 2.794649922188961E-4 16-17 0.0 0.0 0.0 9.98089257924629E-5 3.593121328528665E-4 18-19 0.0 0.0 0.0 1.5969428126794065E-4 3.992357031698516E-4 20-21 0.0 0.0 0.0 1.5969428126794065E-4 4.191974883283442E-4 22-23 0.0 0.0 0.0 1.7965606642643324E-4 4.391592734868368E-4 24-25 0.0 0.0 0.0 2.39541421901911E-4 4.391592734868368E-4 26-27 0.0 0.0 0.0 2.5950320706040356E-4 4.591210586453294E-4 28-29 0.0 0.0 0.0 7.18624265705733E-4 4.79082843803822E-4 30-31 0.0 0.0 0.0 0.00247526135965308 4.79082843803822E-4 32-33 0.0 0.0 0.0 0.006287962324925163 4.79082843803822E-4 34-35 0.0 1.996178515849258E-5 0.0 0.012675733575642788 5.190064141208071E-4 36-37 0.0 3.992357031698516E-5 0.0 0.02295605293226647 5.190064141208071E-4 38-39 0.0 3.992357031698516E-5 0.0 0.03934467854738888 5.190064141208071E-4 40-41 0.0 3.992357031698516E-5 0.0 0.05637208128758305 5.190064141208071E-4 42-43 3.992357031698516E-5 3.992357031698516E-5 0.0 0.07359910187936214 5.190064141208071E-4 44-45 3.992357031698516E-5 3.992357031698516E-5 0.0 0.09789259441724762 5.589299844377922E-4 46-47 3.992357031698516E-5 3.992357031698516E-5 0.0 0.12366325905686154 5.988535547547775E-4 48-49 3.992357031698516E-5 3.992357031698516E-5 0.0 0.15248807682572485 5.988535547547775E-4 50-51 3.992357031698516E-5 3.992357031698516E-5 0.0 0.18199159528997688 5.988535547547775E-4 52-53 3.992357031698516E-5 3.992357031698516E-5 0.0 0.21349129227007818 6.387771250717626E-4 54-55 3.992357031698516E-5 3.992357031698516E-5 0.0 0.24872384307481757 7.18624265705733E-4 56-57 3.992357031698516E-5 3.992357031698516E-5 0.0 0.2868907762978554 7.18624265705733E-4 58-59 3.992357031698516E-5 3.992357031698516E-5 0.0 0.3306070857949541 7.18624265705733E-4 60-61 3.992357031698516E-5 3.992357031698516E-5 0.0 0.3772178541400343 7.18624265705733E-4 62-63 3.992357031698516E-5 3.992357031698516E-5 0.0 0.42853960378251876 7.18624265705733E-4 64-65 3.992357031698516E-5 3.992357031698516E-5 0.0 0.48161799051895055 7.18624265705733E-4 66-67 3.992357031698516E-5 3.992357031698516E-5 0.0 0.5408046835138811 7.585478360227181E-4 68-69 3.992357031698516E-5 3.992357031698516E-5 0.0 0.6080160141425255 7.984714063397032E-4 70-71 3.992357031698516E-5 3.992357031698516E-5 0.0 0.6817748103031556 8.184331914981959E-4 72-73 3.992357031698516E-5 3.992357031698516E-5 0.0 0.7628196580466355 9.182421172906587E-4 74-75 3.992357031698516E-5 3.992357031698516E-5 0.0 0.8452817925363685 9.182421172906587E-4 76-77 3.992357031698516E-5 3.992357031698516E-5 0.0 0.937864552101457 9.182421172906587E-4 78-79 3.992357031698516E-5 3.992357031698516E-5 0.0 1.0446800644845506 9.182421172906587E-4 80-81 3.992357031698516E-5 3.992357031698516E-5 0.0 1.167085731076427 9.98089257924629E-4 82-83 3.992357031698516E-5 3.992357031698516E-5 0.0 1.2972565320949574 0.0011178599688755845 84-85 3.992357031698516E-5 3.992357031698516E-5 0.0 1.4325375501140616 0.0011178599688755845 86-87 3.992357031698516E-5 3.992357031698516E-5 0.0 1.5791169385328727 0.0011577835391925698 88-89 3.992357031698516E-5 3.992357031698516E-5 0.0 1.7419452200706966 0.001197707109509555 90-91 3.992357031698516E-5 3.992357031698516E-5 0.0 1.9271107392008737 0.001197707109509555 92-93 3.992357031698516E-5 3.992357031698516E-5 0.0 2.129583126063464 0.00123763067982654 94-95 3.992357031698516E-5 3.992357031698516E-5 0.0 2.3417170169427646 0.0012775542501435252 96-97 3.992357031698516E-5 3.992357031698516E-5 0.0 2.566646412108659 0.0013174778204605104 98-99 3.992357031698516E-5 3.992357031698516E-5 0.0 2.80804428003031 0.0013574013907774955 100-101 3.992357031698516E-5 3.992357031698516E-5 0.0 3.07712914396679 0.0013574013907774955 102-103 3.992357031698516E-5 3.992357031698516E-5 0.0 3.3784123673639184 0.001437248531411466 104-105 3.992357031698516E-5 3.992357031698516E-5 0.0 3.6935291078758823 0.001477172101728451 106-107 3.992357031698516E-5 3.992357031698516E-5 0.0 4.014514613224444 0.0015170956720454362 108-109 3.992357031698516E-5 3.992357031698516E-5 0.0 4.352387788817088 0.0015570192423624213 110-111 3.992357031698516E-5 3.992357031698516E-5 0.0 4.726491604472399 0.0016368663829963916 112-113 3.992357031698516E-5 3.992357031698516E-5 0.0 5.121176020626113 0.0016368663829963916 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2540 0.0 64.6619 1 GTATCAA 5135 0.0 60.608616 1 TCAACGC 6845 0.0 44.590153 4 CAACGCA 6910 0.0 44.084606 5 AACGCAG 7320 0.0 41.77627 6 TATCAAC 7545 0.0 40.844242 2 ATCAACG 7530 0.0 40.609573 3 GTACATG 13855 0.0 35.47698 1 ACGCAGA 8625 0.0 35.31456 7 ACATGGG 14155 0.0 34.46389 3 TACATGG 14600 0.0 33.576443 2 CGCAGAG 9355 0.0 32.557556 8 CATGGGG 11420 0.0 32.301342 4 GCAGAGT 10145 0.0 29.084654 9 ATGGGGG 5950 0.0 27.89859 5 AGAGTAC 9330 0.0 26.842958 10-11 CAGAGTA 10040 0.0 22.989418 10-11 ATGGGGA 4855 0.0 22.671356 5 GTATTAC 660 0.0 22.540792 1 GTATAGG 730 0.0 22.009695 1 >>END_MODULE