FastQCFastQC Report
Mon 15 Aug 2016
SRR2921543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504786
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33500.073.3107451
GTATCAA62600.055.4196621
TCAACGC87500.038.9192854
CAACGCA89450.038.11765
TATCAAC92800.036.890462
AACGCAG93800.036.468756
ATCAACG94000.036.2280543
GTACATG139050.035.1097831
TACATGG145150.032.9786762
ACATGGG142100.032.8472373
CATGGGG112850.031.3900684
ACGCAGA109600.030.9375237
CGCAGAG117550.028.7928628
ATGGGGG61650.027.1221755
GCAGAGT126450.026.1077449
AGAGTAC117650.025.6610-11
CAGAGTA123850.023.17468610-11
ATGGGGA46500.022.6501775
GTGGTAT12950.021.6343251
GAGTACT53100.021.22888812-13
Produced by FastQC (version 0.11.5)