##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921543_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2504786
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268065615186288	31.0	31.0	34.0	28.0	34.0
2	31.34171621847136	33.0	31.0	34.0	28.0	34.0
3	31.42260137193357	33.0	31.0	34.0	28.0	34.0
4	34.94212519552568	37.0	35.0	37.0	32.0	37.0
5	34.593569670223324	37.0	35.0	37.0	31.0	37.0
6	34.56629748010409	37.0	35.0	37.0	31.0	37.0
7	34.418209779198705	37.0	35.0	37.0	30.0	37.0
8	34.38804073481727	37.0	35.0	37.0	30.0	37.0
9	35.98338420926977	39.0	37.0	39.0	30.0	39.0
10-11	35.8456634618686	39.0	37.0	39.0	30.0	39.0
12-13	35.75527330478532	39.0	37.0	39.0	30.0	39.0
14-15	36.93382328869612	40.0	37.0	41.0	30.0	41.0
16-17	36.9076114686045	40.0	37.0	41.0	30.0	41.0
18-19	36.91246617475505	40.0	37.0	41.0	30.0	41.0
20-21	36.89248961787554	40.0	37.0	41.0	30.0	41.0
22-23	36.82238302992751	40.0	37.0	41.0	30.0	41.0
24-25	36.74682208380277	40.0	37.0	41.0	30.0	41.0
26-27	36.623678829249286	40.0	36.5	41.0	30.0	41.0
28-29	36.43955252065446	40.0	36.0	41.0	29.0	41.0
30-31	36.407134980792776	40.0	36.0	41.0	29.0	41.0
32-33	36.30653457021877	40.0	36.0	41.0	29.0	41.0
34-35	36.1920641124631	40.0	35.5	41.0	29.0	41.0
36-37	36.07263534689191	40.0	35.0	41.0	28.0	41.0
38-39	35.91131178471933	40.0	35.0	41.0	27.5	41.0
40-41	35.725339010997345	40.0	35.0	41.0	27.0	41.0
42-43	35.574181387152436	39.5	35.0	41.0	26.5	41.0
44-45	35.38413640925812	39.0	35.0	41.0	26.0	41.0
46-47	35.15286695150803	39.0	35.0	41.0	24.5	41.0
48-49	34.93633068852988	39.0	35.0	41.0	24.0	41.0
50-51	34.73795346189255	39.0	34.0	41.0	24.0	41.0
52-53	34.54568214609951	38.5	34.0	41.0	23.0	41.0
54-55	34.3289881850186	38.0	34.0	40.5	23.0	41.0
56-57	34.080269332390074	38.0	34.0	40.0	22.5	41.0
58-59	33.94059732048966	37.0	33.5	40.0	22.5	41.0
60-61	33.828032215127365	37.0	34.0	40.0	22.0	41.0
62-63	33.562476594806895	37.0	33.0	40.0	22.0	41.0
64-65	33.26271206402463	36.0	33.0	39.5	21.5	41.0
66-67	32.95886454970604	35.5	33.0	39.0	21.0	41.0
68-69	32.642995649129304	35.0	33.0	39.0	20.0	41.0
70-71	32.34174416497058	35.0	33.0	37.5	20.0	40.0
72-73	32.024113636853606	35.0	32.0	37.0	20.0	39.5
74-75	31.725143385502793	35.0	32.0	37.0	19.0	39.0
76-77	31.428088267820087	35.0	32.0	36.0	18.0	39.0
78-79	31.147944175670098	35.0	32.0	36.0	18.0	37.5
80-81	30.918794859121697	35.0	32.0	35.0	17.0	37.0
82-83	30.72439781282712	35.0	32.0	35.0	15.5	37.0
84-85	30.530605808240704	35.0	31.0	35.0	14.0	36.0
86-87	30.38633480065762	35.0	31.0	35.0	12.0	36.0
88-89	30.27922485194344	35.0	31.0	35.0	10.5	36.0
90-91	30.180390061266714	35.0	31.0	35.0	10.0	35.0
92-93	30.244833690383132	35.0	31.5	35.0	10.0	35.0
94-95	30.216173357723974	35.0	32.0	35.0	9.0	35.0
96-97	30.19691163237099	35.0	32.0	35.0	7.0	35.0
98-99	30.157440396105695	35.0	32.0	35.0	7.0	35.0
100-101	30.11169537038294	35.0	32.0	35.0	7.0	35.0
102-103	29.221760861007688	33.5	29.5	35.0	5.0	35.0
104-105	29.936181374376893	34.5	31.0	35.0	2.0	35.0
106-107	30.046625739683947	35.0	32.0	35.0	2.0	35.0
108-109	30.05467133719208	35.0	32.0	35.0	2.0	35.0
110-111	30.020929931738678	35.0	32.0	35.0	2.0	35.0
112-113	29.976916790496276	35.0	32.0	35.0	2.0	35.0
114-115	29.914458959767423	35.0	32.0	35.0	2.0	35.0
116-117	29.839384482346993	35.0	32.0	35.0	2.0	35.0
118-119	29.747202156192188	35.0	32.0	35.0	2.0	35.0
120-121	29.622310448876668	35.0	31.0	35.0	2.0	35.0
122-123	29.50335118449241	35.0	31.5	35.0	2.0	35.0
124-125	28.5825084458313	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41829.0
3	15600.0
4	8974.0
5	7396.0
6	7621.0
7	8746.0
8	10097.0
9	11193.0
10	12602.0
11	13259.0
12	13700.0
13	14963.0
14	18266.0
15	15061.0
16	9156.0
17	7973.0
18	7254.0
19	6833.0
20	6798.0
21	7267.0
22	8526.0
23	11534.0
24	15971.0
25	21639.0
26	27333.0
27	31489.0
28	36976.0
29	44315.0
30	51641.0
31	62309.0
32	78279.0
33	100470.0
34	139640.0
35	221543.0
36	440153.0
37	730902.0
38	237195.0
39	283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.843532955577444	18.1427552730203	13.18366994538315	24.83004182601911
2	16.61280219168194	20.130927604149406	37.631485545622844	25.624784658545806
3	18.21254780880153	25.162783528455275	29.513753840477335	27.110914822265862
4	13.357745670951143	16.476450429599353	35.605755261188975	34.560048638260525
5	14.471868276156203	37.27491346358123	33.38644521010739	14.866773050155183
6	32.94040884910662	35.26058001084535	17.40024549793994	14.398765642108089
7	29.161277854287043	31.445402263281235	21.244105913671724	18.149213968759994
8	27.904038034718496	32.96105378017526	19.7192911290519	19.415617056054337
9	27.914747270524863	14.119010146548842	19.028072890341097	38.9381696925852
10-11	26.0687718334411	24.83906212350758	27.28857982392906	21.803586219122256
12-13	26.557653123251395	22.76743855037393	27.38468706599882	23.290221260375855
14-15	24.23321860341443	23.77406603444772	24.81779422315362	27.17492113898423
16-17	24.084250747889588	26.497141699249067	25.34086496662571	24.077742586235637
18-19	24.111338597641364	25.742035065753278	26.95421265749605	23.19241367910931
20-21	25.50519736241868	24.62306220643991	26.485156767351775	23.386583663789636
22-23	25.28884716069854	24.349823133206378	26.247595144632697	24.113734561462383
24-25	24.588987342353395	24.775015876710878	26.36857647044496	24.267420310490767
26-27	24.21313147122754	24.907308624150946	26.517699001975608	24.36186090264591
28-29	24.359837952056424	24.9074445266095	26.539423520749516	24.19329400058456
30-31	24.72221312660277	24.794130636699574	26.27614646733683	24.207509769360826
32-33	24.43051856306146	24.87730337545237	26.234547751911148	24.457630309575016
34-35	24.57763970194788	24.891744467427504	26.361839425618744	24.16877640500587
36-37	24.73146273300482	24.677417873575358	26.202792603900093	24.38832678951973
38-39	24.71773177540574	24.950195007161778	26.04043418692975	24.291639030502736
40-41	24.719828567184845	24.788244378803594	26.25011079887788	24.24181625513368
42-43	24.611152934966597	24.902063303549557	25.991100602243556	24.495683159240286
44-45	24.672141130151275	24.87586624100816	25.889480524925577	24.56251210391498
46-47	24.814125503418413	24.924751774692158	25.726667459944146	24.534455261945283
48-49	24.8248744297001	25.159402453684688	25.423630676269717	24.592092440345496
50-51	24.74692208744033	25.30651351322753	25.28666857130194	24.6598958280302
52-53	24.89122003472094	25.164075906882395	25.331670827921705	24.61303323047496
54-55	24.843927720700847	25.072727915240904	25.45631763057234	24.627026733485906
56-57	24.683616206117815	25.185497687655495	25.298604207642406	24.83228189858428
58-59	24.70379306592769	25.009698038131305	25.507108067989087	24.77940082795191
60-61	24.877371215817874	25.153056375698498	25.49135369240021	24.47821871608342
62-63	24.70152151798398	25.20330998295892	25.514322195234683	24.580846303822412
64-65	24.827479495595643	24.93373503597754	25.51546515249527	24.723320315931545
66-67	24.77790665870755	25.12688338627212	25.356293659524088	24.738916295496242
68-69	24.763401869703756	25.06471644463577	25.40883921310394	24.763042472556535
70-71	24.57287801605769	24.906130491942704	25.671081882640905	24.8499096093587
72-73	24.843616575455066	25.16228317903129	25.3494780963379	24.644622149175742
74-75	24.703805934762535	25.311817115475804	25.165888841747986	24.818488108013675
76-77	24.67915213591124	25.04264395729535	25.31723430778236	24.960969599011044
78-79	24.619083836399387	25.1133208988677	25.34384638715942	24.923748877573495
80-81	24.661292766798397	25.21860210852328	25.1912487678518	24.928856356826525
82-83	24.769795491555126	25.094741944364422	25.25139580043093	24.884066763649518
84-85	24.585964550120575	25.156725057701934	25.15420955090018	25.10310084127731
86-87	24.475056499205415	25.287407265438453	25.27860297229742	24.958933263058704
88-89	24.527910856168518	25.01823387477576	25.319719239258585	25.134136029797137
90-91	24.433415673122287	25.101204471463802	25.355690519251763	25.109689336162155
92-93	24.524364936531086	25.19290218190929	25.302501724346172	24.980231157213456
94-95	24.478287351152066	25.004097441858747	25.33352977098444	25.184085436004743
96-97	24.44847330415759	25.008698623970517	25.37319242793517	25.16963564393673
98-99	24.324122005380193	25.071684029732406	25.236830779377676	25.367363185509724
100-101	24.33485945938284	25.075249866290118	25.435642672888026	25.154248001439015
102-103	24.400014293097534	24.865917370325576	25.50705062930552	25.227017707271376
104-105	24.26901273550951	25.061393867360497	25.375639440434473	25.293953956695514
106-107	24.27566435029372	25.041560569439685	25.308650107913756	25.374124972352842
108-109	24.29709385798304	24.940427452866558	25.28487507185143	25.47760361729897
110-111	24.220332449752362	25.13484773748642	25.301030693642666	25.343789119118547
112-113	24.173365591717694	25.047528147064057	25.433003728985653	25.3461025322326
114-115	24.06333441927612	25.198424105374777	25.322668736345864	25.415572739003235
116-117	23.995853191148285	25.202903564734395	25.27792622824657	25.523317015870745
118-119	24.059003579918915	24.898284146381393	25.39133153339195	25.651380740307744
120-121	23.878135067359608	25.01550091408481	25.506040465670214	25.600323552885367
122-123	23.82129943216366	25.0494556188969	25.41623565587433	25.713009293065113
124-125	23.759195828595445	25.14323479524837	25.40920875691465	25.68836061924153
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	17.5
2	37.5
3	63.5
4	52.5
5	43.0
6	65.0
7	145.0
8	259.0
9	321.0
10	311.5
11	289.0
12	334.0
13	411.5
14	506.5
15	624.0
16	739.5
17	866.5
18	1026.5
19	1159.0
20	1346.0
21	1599.5
22	1842.0
23	2138.0
24	2521.0
25	3235.5
26	4432.5
27	5869.0
28	7181.5
29	9207.5
30	12503.5
31	15553.5
32	19361.5
33	24000.0
34	28718.0
35	33566.5
36	39170.0
37	44967.5
38	48908.5
39	53575.5
40	58419.5
41	62017.0
42	66064.5
43	72528.0
44	79775.0
45	85306.5
46	89986.0
47	94205.0
48	99208.0
49	103971.5
50	103762.0
51	102803.5
52	102957.5
53	101824.0
54	101577.5
55	100158.0
56	95054.5
57	86835.0
58	80768.5
59	75885.0
60	68715.0
61	61022.0
62	52284.5
63	41275.5
64	31365.0
65	24683.0
66	19457.5
67	15230.0
68	12223.0
69	9909.5
70	8325.5
71	6868.5
72	5463.0
73	4880.0
74	4007.5
75	3108.5
76	2276.0
77	1404.0
78	887.0
79	551.0
80	337.5
81	185.0
82	104.0
83	54.5
84	26.5
85	17.5
86	14.5
87	13.0
88	6.5
89	2.5
90	1.5
91	0.5
92	1.5
93	1.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11953116952905357
2	0.001716713523630362
3	0.021558727971171986
4	0.05617246343599812
5	0.025471237862236533
6	0.01948270231468876
7	0.014731797446967525
8	0.0050702934302571155
9	0.0036729684691626354
10-11	0.00680696873904597
12-13	0.0044914016606608315
14-15	0.002515184929970065
16-17	0.009521771520600962
18-19	0.00479082843803822
20-21	0.01175749145835213
22-23	0.008683376543944273
24-25	0.013234663560080583
26-27	0.009501809735442469
28-29	0.013653861048408926
30-31	0.021079645127368166
32-33	0.013434281411665508
34-35	0.020999797986734195
36-37	0.015370574572039287
38-39	0.0207203329945153
40-41	0.00990104543861232
42-43	0.00936207723933302
44-45	0.017207058806620604
46-47	0.01628881668932995
48-49	0.02042090621713791
50-51	0.01475175923212602
52-53	0.021299224764111585
54-55	0.016668090607341306
56-57	0.02034105907650394
58-59	0.016508396326073364
60-61	0.01916331375215288
62-63	0.02169846046728144
64-65	0.017805912361375385
66-67	0.012815466071752237
68-69	0.02365471541281371
70-71	0.015111071364978884
72-73	0.017746027005899907
74-75	0.018903810545092475
76-77	0.013094931063971133
78-79	0.02892462669465575
80-81	0.020820141920307764
82-83	0.008763223684578244
84-85	0.012795504286593746
86-87	0.013015083923337164
88-89	0.02070037120935681
90-91	0.013035045708495655
92-93	0.008523682262676332
94-95	0.006966663020313911
96-97	0.0031739238402003207
98-99	0.001756637093947347
100-101	0.0118373385989861
102-103	0.0032138474105173056
104-105	0.0022756435080681543
106-107	3.193885625358813E-4
108-109	0.019422816959213284
110-111	6.587389102302552E-4
112-113	0.008683376543944273
114-115	0.001996178515849258
116-117	0.008363987981408392
118-119	0.010380128282416141
120-121	0.0037927391801135905
122-123	5.988535547547775E-4
124-125	0.023694638983130695
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2504786.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.19836417508846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.05405040680486	42.30401849288722
2	11.29816036763086	12.246836202268172
3	3.924766516161312	6.38147774835312
4	1.846987454016022	4.004147946383192
5	1.107585715954319	3.0014666994209134
6	0.7424567581341052	2.4143965056964682
7	0.5222524234757565	1.9813658927203097
8	0.3756157174232691	1.6286205954234765
9	0.2903185602023932	1.4161311947372985
>10	1.6885600051577108	16.71451598239825
>50	0.10835879726912405	4.009422006314368
>100	0.040443878345688244	3.6061384059720134
>500	1.4779980821967863E-4	0.04726199620225434
>1k	2.9559961643695705E-4	0.24420033122295406
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.992357031698516E-5
5	0.0	0.0	0.0	0.0	3.992357031698516E-5
6	0.0	0.0	0.0	0.0	3.992357031698516E-5
7	0.0	0.0	0.0	0.0	3.992357031698516E-5
8	0.0	0.0	0.0	0.0	3.992357031698516E-5
9	0.0	0.0	0.0	3.992357031698516E-5	1.197707109509555E-4
10-11	0.0	0.0	0.0	5.988535547547775E-5	1.197707109509555E-4
12-13	0.0	0.0	0.0	7.984714063397033E-5	1.197707109509555E-4
14-15	0.0	0.0	0.0	7.984714063397033E-5	1.5969428126794065E-4
16-17	0.0	0.0	0.0	1.3973249610944806E-4	2.195796367434184E-4
18-19	0.0	0.0	0.0	1.5969428126794065E-4	2.39541421901911E-4
20-21	0.0	0.0	0.0	1.5969428126794065E-4	2.794649922188961E-4
22-23	0.0	0.0	0.0	1.7965606642643324E-4	3.393503476943739E-4
24-25	0.0	0.0	0.0	2.39541421901911E-4	3.593121328528665E-4
26-27	0.0	0.0	0.0	2.794649922188961E-4	3.593121328528665E-4
28-29	0.0	0.0	0.0	6.986624805472403E-4	3.593121328528665E-4
30-31	0.0	0.0	0.0	0.002595032070604036	3.593121328528665E-4
32-33	0.0	0.0	0.0	0.006467618391351597	3.7927391801135905E-4
34-35	0.0	0.0	0.0	0.012955198567861686	3.992357031698516E-4
36-37	0.0	0.0	0.0	0.023475059346387276	4.391592734868368E-4
38-39	0.0	0.0	0.0	0.04018307352404557	4.990446289623146E-4
40-41	0.0	0.0	0.0	0.0573102851900322	5.190064141208071E-4
42-43	0.0	0.0	0.0	0.0747968089888717	5.190064141208071E-4
44-45	0.0	0.0	0.0	0.09923003402286662	5.389681992792997E-4
46-47	0.0	0.0	0.0	0.12577920828366176	5.988535547547774E-4
48-49	0.0	0.0	0.0	0.15530268853307228	6.387771250717626E-4
50-51	0.0	0.0	0.0	0.18506571020438473	6.387771250717626E-4
52-53	0.0	0.0	0.0	0.21674506325091247	7.385860508642254E-4
54-55	0.0	0.0	0.0	0.2521772319072368	7.984714063397032E-4
56-57	0.0	0.0	0.0	0.29046393584122554	7.984714063397032E-4
58-59	0.0	0.0	0.0	0.33495875495950556	7.984714063397032E-4
60-61	0.0	0.0	0.0	0.3819088736522801	8.184331914981959E-4
62-63	0.0	0.0	0.0	0.43366978256825134	9.182421172906587E-4
64-65	0.0	0.0	0.0	0.48662839859373214	9.58165687607644E-4
66-67	0.0	1.996178515849258E-5	0.0	0.5469129897723797	9.781274727661366E-4
68-69	0.0	3.992357031698516E-5	0.0	0.6152022567995828	9.98089257924629E-4
70-71	0.0	3.992357031698516E-5	0.0	0.690178721854881	9.98089257924629E-4
72-73	0.0	3.992357031698516E-5	0.0	0.7719821174343837	0.0011178599688755845
74-75	0.0	3.992357031698516E-5	0.0	0.8561210418774299	0.0011378217540340772
76-77	0.0	3.992357031698516E-5	0.0	0.949861584981711	0.0011577835391925698
78-79	0.0	3.992357031698516E-5	0.0	1.0580943841110577	0.0011577835391925698
80-81	0.0	3.992357031698516E-5	0.0	1.1823365349375157	0.0011577835391925698
82-83	0.0	3.992357031698516E-5	0.0	1.3142839348351516	0.001197707109509555
84-85	0.0	3.992357031698516E-5	0.0	1.451640978510739	0.001197707109509555
86-87	0.0	3.992357031698516E-5	0.0	1.5999770040234975	0.00123763067982654
88-89	0.0	3.992357031698516E-5	0.0	1.7647016551513781	0.0012775542501435252
90-91	0.0	3.992357031698516E-5	0.0	1.9519831235083558	0.0012775542501435252
92-93	0.0	3.992357031698516E-5	0.0	2.157789128492414	0.0012775542501435252
94-95	0.0	3.992357031698516E-5	0.0	2.3740551088995225	0.0012775542501435252
96-97	0.0	3.992357031698516E-5	0.0	2.603036746452591	0.0013973249610944806
98-99	0.0	3.992357031698516E-5	0.0	2.848686474613001	0.001437248531411466
100-101	0.0	3.992357031698516E-5	0.0	3.1228815555500553	0.001437248531411466
102-103	0.0	3.992357031698516E-5	0.0	3.429055416311014	0.0016169045978378991
104-105	0.0	3.992357031698516E-5	0.0	3.747705392796031	0.001676789953313377
106-107	0.0	3.992357031698516E-5	0.0	4.072303981258279	0.0016967517384718694
108-109	0.0	3.992357031698516E-5	0.0	4.4183016034104305	0.0017366753087888548
110-111	0.0	3.992357031698516E-5	0.0	4.8017874580902316	0.001816522449422825
112-113	0.0	3.992357031698516E-5	0.0	5.204676167944088	0.0018764078048983026
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3350	0.0	73.310745	1
GTATCAA	6260	0.0	55.419662	1
TCAACGC	8750	0.0	38.919285	4
CAACGCA	8945	0.0	38.1176	5
TATCAAC	9280	0.0	36.89046	2
AACGCAG	9380	0.0	36.46875	6
ATCAACG	9400	0.0	36.228054	3
GTACATG	13905	0.0	35.109783	1
TACATGG	14515	0.0	32.978676	2
ACATGGG	14210	0.0	32.847237	3
CATGGGG	11285	0.0	31.390068	4
ACGCAGA	10960	0.0	30.937523	7
CGCAGAG	11755	0.0	28.792862	8
ATGGGGG	6165	0.0	27.122175	5
GCAGAGT	12645	0.0	26.107744	9
AGAGTAC	11765	0.0	25.66	10-11
CAGAGTA	12385	0.0	23.174686	10-11
ATGGGGA	4650	0.0	22.650177	5
GTGGTAT	1295	0.0	21.634325	1
GAGTACT	5310	0.0	21.228888	12-13
>>END_MODULE