FastQCFastQC Report
Mon 15 Aug 2016
SRR2921542_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921542_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2142519
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21800.073.1606451
GTATCAA46500.058.6154021
TCAACGC59500.045.09594
CAACGCA61850.043.6700335
ATCAACG63900.041.895653
AACGCAG65350.041.2391626
GTACATG111200.039.4957921
TATCAAC69400.038.746832
TACATGG118050.036.6374862
ACATGGG119650.036.0978363
ACGCAGA75050.035.7480747
CATGGGG98650.034.2553754
CGCAGAG79400.033.8637168
GCAGAGT88600.029.675999
ATGGGGG51850.029.2589555
AGAGTAC78650.026.8116810-11
CAGAGTA82150.025.37972810-11
ATGGGGA38550.024.8467065
GTATAGA6650.022.3726581
GTATAGG6150.021.2885841
Produced by FastQC (version 0.11.5)