##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921542_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2142519
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.881509568876634	31.0	30.0	34.0	26.0	34.0
2	30.016743842178297	31.0	30.0	34.0	25.0	34.0
3	30.102811690351405	31.0	30.0	34.0	26.0	34.0
4	33.50896864858608	37.0	35.0	37.0	27.0	37.0
5	33.52115897221915	37.0	35.0	37.0	27.0	37.0
6	33.555523661633806	37.0	35.0	37.0	28.0	37.0
7	33.41845463214095	37.0	35.0	37.0	27.0	37.0
8	33.35959681104345	37.0	35.0	37.0	27.0	37.0
9	34.84427489324482	39.0	35.0	39.0	27.0	39.0
10-11	34.7175387942884	39.0	35.0	39.0	27.0	39.0
12-13	34.5724537331991	39.0	35.0	39.0	25.5	39.0
14-15	35.723045629933736	40.0	36.0	41.0	26.0	41.0
16-17	35.63690543701129	40.0	36.0	41.0	25.0	41.0
18-19	35.64865982518708	40.0	36.0	41.0	25.0	41.0
20-21	35.660881420421475	40.0	36.0	41.0	25.0	41.0
22-23	35.61698846077911	40.0	36.0	41.0	25.0	41.0
24-25	35.567068483406686	40.0	36.0	41.0	25.0	41.0
26-27	35.485438635550025	40.0	35.0	41.0	25.0	41.0
28-29	35.43322999702687	40.0	35.0	41.0	25.0	41.0
30-31	35.3323933183323	40.0	35.0	41.0	25.0	41.0
32-33	35.224013649353864	40.0	35.0	41.0	24.5	41.0
34-35	35.05785129560111	40.0	35.0	41.0	24.0	41.0
36-37	34.9241647331949	39.5	35.0	41.0	24.0	41.0
38-39	34.7939150131224	39.0	34.5	41.0	23.0	41.0
40-41	34.64824839359651	39.0	34.0	41.0	22.0	41.0
42-43	34.45720668054752	39.0	34.0	41.0	20.5	41.0
44-45	34.261942601209135	39.0	33.5	41.0	19.0	41.0
46-47	34.01404421617731	39.0	33.0	41.0	18.0	41.0
48-49	33.878012050301535	38.0	33.0	41.0	16.0	41.0
50-51	33.68247166069472	38.0	33.0	40.0	15.0	41.0
52-53	33.491255620136855	38.0	33.0	40.0	13.5	41.0
54-55	33.28237509212287	38.0	33.0	40.0	12.5	41.0
56-57	33.04028248991024	37.0	32.5	40.0	9.5	41.0
58-59	32.798831422265096	37.0	32.0	40.0	8.0	41.0
60-61	32.49494893627548	36.0	32.0	40.0	7.5	41.0
62-63	32.18457222549718	36.0	32.0	39.0	7.0	41.0
64-65	31.874243588971673	35.0	31.0	39.0	4.0	41.0
66-67	31.83895848764935	35.0	32.0	39.0	2.0	41.0
68-69	31.59205846015835	35.0	31.5	38.5	2.0	40.5
70-71	31.297970986488334	35.0	31.0	37.5	2.0	40.0
72-73	30.968931664083257	35.0	31.0	37.0	2.0	39.0
74-75	30.66642629540275	35.0	31.0	36.5	2.0	39.0
76-77	30.391785090353924	35.0	31.0	36.0	2.0	39.0
78-79	30.1202267051074	35.0	31.0	35.5	2.0	37.0
80-81	29.88056675343369	35.0	30.0	35.0	2.0	37.0
82-83	29.685269068792387	35.0	30.0	35.0	2.0	36.5
84-85	29.48154135389231	35.0	30.0	35.0	2.0	36.0
86-87	29.3355132439899	35.0	30.0	35.0	2.0	36.0
88-89	29.215429128049742	34.0	30.0	35.0	2.0	35.5
90-91	29.10878409946423	34.0	30.0	35.0	2.0	35.0
92-93	29.018210340258356	34.0	30.0	35.0	2.0	35.0
94-95	28.955441235293595	34.0	30.0	35.0	2.0	35.0
96-97	28.859658187395304	34.0	30.0	35.0	2.0	35.0
98-99	28.801485074344733	34.0	29.5	35.0	2.0	35.0
100-101	28.738630089161404	34.0	29.0	35.0	2.0	35.0
102-103	27.762044817338843	32.5	28.0	34.5	2.0	35.0
104-105	28.596865418696403	34.0	29.5	35.0	2.0	35.0
106-107	28.92477382931027	34.5	30.0	35.0	2.0	35.0
108-109	28.971070968332135	35.0	30.0	35.0	2.0	35.0
110-111	28.912078959393124	35.0	30.0	35.0	2.0	35.0
112-113	28.847456195254278	35.0	30.0	35.0	2.0	35.0
114-115	28.734188588292568	35.0	30.0	35.0	2.0	35.0
116-117	28.680666309143582	35.0	30.0	35.0	2.0	35.0
118-119	28.529108259950085	35.0	30.0	35.0	2.0	35.0
120-121	28.362957341335132	34.5	30.0	35.0	2.0	35.0
122-123	28.248786125117213	34.5	30.0	35.0	2.0	35.0
124-125	27.404479726900902	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80909.0
3	14447.0
4	9754.0
5	8395.0
6	8676.0
7	9916.0
8	11019.0
9	11361.0
10	12144.0
11	12594.0
12	12643.0
13	13026.0
14	14436.0
15	11931.0
16	9114.0
17	7842.0
18	7284.0
19	6718.0
20	6748.0
21	6866.0
22	8302.0
23	11276.0
24	15666.0
25	21635.0
26	25951.0
27	27950.0
28	32986.0
29	39304.0
30	45459.0
31	54514.0
32	68999.0
33	89715.0
34	126232.0
35	199928.0
36	378925.0
37	561339.0
38	158420.0
39	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.089867469120094	17.524985234141	12.60805924085976	24.777088055879144
2	16.61950565675344	19.44253678768862	38.766910385055134	25.17104717050281
3	18.574257111417065	24.75448693079134	29.041248996822045	27.63000696096955
4	13.190948967133629	15.553900707564239	36.08965126023937	35.16549906506276
5	14.34630400699223	37.1013994731549	33.55971549243956	14.992581027413312
6	32.96587225466509	34.993243186451636	17.743149562324717	14.297734996558553
7	29.706541131821396	31.037656251240275	21.23200522211011	18.023797394828225
8	27.663037464404823	33.2322498019183	19.719879558855595	19.384833174821285
9	27.88416915591055	14.538195848324046	19.11767314912773	38.459961846637675
10-11	26.085515211476856	24.805896615464494	27.279387011963465	21.829201161095188
12-13	26.673450768802653	22.678604445291096	27.127957597727487	23.51998718817876
14-15	24.285301747996016	23.77404845607342	24.474838984394168	27.465810811536397
16-17	24.206130760865964	26.559222171329427	24.9864713877438	24.24817568006081
18-19	24.298039614899505	25.883564864646523	26.413948942477283	23.404446577976685
20-21	25.588199168798997	24.801271600227718	25.929006523793003	23.68152270718028
22-23	25.388162718259803	24.481360063884335	25.685589132191073	24.444888085664786
24-25	24.76654359351345	24.996724873239167	25.771733122355233	24.46499841089215
26-27	24.358965378923827	25.036971766038647	25.981427028450632	24.62263582658689
28-29	24.49374033967004	24.901633909100674	26.062087611896388	24.542538139332894
30-31	24.83742491146277	24.87751415848538	25.800267292778052	24.4847936372738
32-33	24.4428431461371	25.01846993300388	25.821316590996762	24.717370329862263
34-35	24.76093511723051	25.012046628858307	25.868267778816424	24.358750475094762
36-37	24.891373760888015	24.769631117923886	25.700656462295157	24.638338658892945
38-39	24.82125852492573	25.065955359115193	25.646161699329497	24.466624416629575
40-41	24.76467297505286	24.90245100602945	25.925337895892948	24.40753812302474
42-43	24.70695702550085	24.906697227804937	25.70820324184373	24.678142504850484
44-45	24.670417094178354	24.94520767377445	25.616329023890994	24.7680462081562
46-47	24.785733200880465	24.97640274638872	25.585727970019693	24.652136082711117
48-49	24.823504772498197	25.234153720648045	25.27991628252965	24.662425224324107
50-51	24.740710888788435	25.34283133902921	25.176727535593006	24.739730236589345
52-53	24.882846943045447	25.17506407574014	25.346835629847714	24.5952533513667
54-55	24.869444701919264	25.098767210570223	25.419473174613145	24.61231491289737
56-57	24.736653852098435	25.183171435202066	25.255151236964608	24.82502347573489
58-59	24.792276622072798	25.013751721883683	25.32555859074035	24.86841306530317
60-61	24.807334771999972	25.249743439857543	25.434914963969526	24.508006824172963
62-63	24.701777126759723	25.30114124793437	25.404638488085794	24.59244313722011
64-65	24.73120714676608	24.99059119952634	25.497032142143393	24.781169511564187
66-67	24.730385794139785	25.174108818712053	25.309161046688615	24.786344340459546
68-69	24.687403632217706	25.157835983172568	25.37533390962386	24.779426474985872
70-71	24.681338006793517	24.950687710312714	25.586797568381108	24.78117671451266
72-73	24.890596862804053	25.179622839892073	25.27601152088686	24.653768776417014
74-75	24.594820169750513	25.43829647156603	25.23373671744863	24.73314664123483
76-77	24.684924439526526	25.13463385823555	25.355473304923017	24.82496839731491
78-79	24.662806580060224	25.15897003246516	25.336247968738945	24.841975418735668
80-81	24.665369117553002	25.34478500569798	25.19844891682349	24.791396959925528
82-83	24.887850037544766	25.19572412147932	25.169161520454487	24.74726432052143
84-85	24.610706643913492	25.21963654204938	25.158622177064434	25.011034636972695
86-87	24.42231752684352	25.323940532281547	25.376551546423038	24.877190394451894
88-89	24.62877452058261	25.131125798134196	25.30908807405322	24.931011607229976
90-91	24.56255044352962	25.13267611064457	25.42339888911036	24.88137455671545
92-93	24.681714800028384	25.31324342795681	25.217379735064217	24.787662036950586
94-95	24.534301677751	25.030820931438658	25.413275897401427	25.02160149340892
96-97	24.673522692077977	25.10583832660264	25.297011320307305	24.923627661012077
98-99	24.55202394433651	25.084955342430714	25.218573291720777	25.144447421512
100-101	24.481553254848727	25.173500207838483	25.396089275557426	24.94885726175536
102-103	24.607453866391836	24.946304165228437	25.509623241130296	24.93661872724943
104-105	24.437251532986338	25.216382474314862	25.36360144082278	24.982764551876016
106-107	24.490290803284005	25.17233033557708	25.281137381381765	25.05624147975715
108-109	24.51067300025954	25.1291412492041	25.249716650141814	25.110469100394546
110-111	24.490561801581368	25.26969365209653	25.307245268064626	24.932499278257474
112-113	24.462933439384827	25.16179037512682	25.446757085833795	24.92851909965456
114-115	24.272958582339836	25.374647524420645	25.42517622028551	24.927217672954008
116-117	24.374795348210863	25.467562968569453	25.250605815973376	24.907035867246307
118-119	24.479750044213876	24.99309889217335	25.39179577586699	25.135355287745785
120-121	24.162917165435623	25.14919739868032	25.582813134195803	25.105072301688253
122-123	24.26529548435652	25.240084905984105	25.43813350632628	25.056486103333093
124-125	24.22024836110145	25.2934847802394	25.46896186366511	25.01730499499404
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	66.5
2	93.0
3	112.0
4	108.5
5	103.0
6	101.5
7	128.5
8	195.5
9	232.0
10	246.5
11	281.5
12	344.0
13	401.5
14	465.0
15	573.5
16	655.0
17	730.5
18	810.5
19	905.5
20	1079.0
21	1223.5
22	1399.0
23	1645.5
24	1911.5
25	2541.0
26	3868.0
27	5333.0
28	6371.0
29	8192.5
30	11345.0
31	14207.5
32	17828.0
33	22325.5
34	27047.0
35	31661.0
36	36851.5
37	42162.5
38	44611.5
39	47054.0
40	49279.5
41	50781.0
42	53355.0
43	58632.5
44	64093.0
45	67433.5
46	71278.0
47	74945.5
48	79794.0
49	85035.0
50	86106.5
51	85099.0
52	84372.0
53	84940.5
54	86581.0
55	86589.0
56	83137.5
57	76770.5
58	72555.0
59	68000.0
60	60556.0
61	53574.0
62	46605.0
63	37150.0
64	28272.5
65	22467.5
66	17542.0
67	13561.5
68	11079.0
69	9221.5
70	7932.5
71	6618.5
72	5357.5
73	4892.5
74	4102.0
75	3179.5
76	2298.5
77	1464.5
78	945.0
79	624.0
80	388.0
81	201.0
82	115.5
83	65.0
84	46.5
85	39.0
86	27.0
87	19.0
88	16.0
89	10.5
90	9.0
91	11.0
92	10.0
93	6.5
94	6.0
95	8.0
96	9.5
97	8.5
98	6.5
99	6.0
100	27.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035192219998982505
2	0.026417502015151324
3	0.02688424233344022
4	0.03099155713438247
5	0.033278584693998046
6	0.04560052909682481
7	0.03995297124552921
8	0.033885347107773606
9	0.02973135827500246
10-11	0.03491217580800917
12-13	0.03486550177618028
14-15	0.033768662028201384
16-17	0.03556561225361362
18-19	0.04910108148399151
20-21	0.059742760740978264
22-23	0.05362846257139376
24-25	0.06443350093978163
26-27	0.04760751246546705
28-29	0.04802757875192706
30-31	0.04886771132484706
32-33	0.05605551222649601
34-35	0.03925286076809587
36-37	0.052111556536954866
38-39	0.06058289331389826
40-41	0.04812092681558484
42-43	0.05775911438825047
44-45	0.058435887849769365
46-47	0.06441016392386718
48-49	0.04809758979967039
50-51	0.05073467259800263
52-53	0.06081626347304272
54-55	0.046370650622001484
56-57	0.04415363411012925
58-59	0.0452271368421937
60-61	0.03365197694862916
62-63	0.042753413155262564
64-65	0.042333346868802565
66-67	0.035238894030811395
68-69	0.03078152399115247
70-71	0.04970784389776707
72-73	0.03127160132535581
74-75	0.039509567943154765
76-77	0.032275093009676924
78-79	0.031948374786874706
80-81	0.024830584932969087
82-83	0.019113016033930154
84-85	0.01869294974747015
86-87	0.017829480158635697
88-89	0.02296362365981352
90-91	0.012695336657457881
92-93	0.019369723208989047
94-95	0.014282253739640116
96-97	0.010198275954612304
98-99	0.01012826490686897
100-101	0.010758364336558976
102-103	0.005950939058183381
104-105	0.006160972201413384
106-107	0.009078099190718962
108-109	0.013582143262206775
110-111	0.006277657280985606
112-113	0.0076312042040233945
114-115	0.008027933474568953
116-117	0.007491182108536727
118-119	0.2418181589054753
120-121	0.3584332274299551
122-123	0.028961236749825793
124-125	0.0034538783553378057
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2142519.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.72032608763935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.7941429292062	43.66361520969865
2	10.244751420334328	11.211922768150016
3	3.5247505385645512	5.7862649654350395
4	1.766531413515338	3.86660699966471
5	1.028816393660384	2.8148584272702672
6	0.7008775965959279	2.301135037995007
7	0.5125310724349741	1.9632107189582495
8	0.36003823381263295	1.576112764659601
9	0.26318806797673155	1.2961563211856286
>10	1.6471387538212767	16.470916180791686
>50	0.10979396314028991	4.1182182457336145
>100	0.04632310458897649	4.460825759249518
>500	8.588556526690149E-4	0.28414302388087065
>1k	2.576566958006915E-4	0.18601357732723095
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.667403182888926E-5	0.0
5	0.0	0.0	0.0	4.667403182888926E-5	0.0
6	0.0	0.0	0.0	4.667403182888926E-5	0.0
7	0.0	0.0	0.0	4.667403182888926E-5	0.0
8	0.0	0.0	0.0	4.667403182888926E-5	0.0
9	0.0	0.0	0.0	4.667403182888926E-5	9.334806365777853E-5
10-11	0.0	0.0	0.0	4.667403182888926E-5	1.1668507957222316E-4
12-13	0.0	0.0	0.0	4.667403182888926E-5	1.6335911140111242E-4
14-15	0.0	0.0	0.0	4.667403182888926E-5	1.8669612731555705E-4
16-17	0.0	0.0	0.0	4.667403182888926E-5	1.8669612731555705E-4
18-19	0.0	0.0	0.0	4.667403182888926E-5	1.8669612731555705E-4
20-21	0.0	0.0	0.0	7.00110477433339E-5	2.3337015914444632E-4
22-23	0.0	0.0	0.0	1.6335911140111242E-4	2.3337015914444632E-4
24-25	0.0	0.0	0.0	2.3337015914444632E-4	2.3337015914444632E-4
26-27	0.0	0.0	0.0	3.9672927054555874E-4	2.3337015914444632E-4
28-29	0.0	0.0	0.0	0.001143513779807787	2.3337015914444632E-4
30-31	0.0	0.0	0.0	0.0031971711802789146	2.3337015914444632E-4
32-33	0.0	0.0	0.0	0.007421171060793394	2.5670717505889095E-4
34-35	0.0	0.0	0.0	0.013278762055318995	2.800441909733356E-4
36-37	0.0	0.0	0.0	0.0217500988322624	2.800441909733356E-4
38-39	0.0	0.0	0.0	0.036125700635560296	3.2671822280222484E-4
40-41	0.0	0.0	0.0	0.054375247080656	3.2671822280222484E-4
42-43	0.0	0.0	0.0	0.07029109193430723	3.500552387166695E-4
44-45	0.0	0.0	0.0	0.09017422949341405	3.733922546311141E-4
46-47	0.0	0.0	0.0	0.11187765429384758	3.9672927054555874E-4
48-49	0.0	0.0	0.0	0.13591478068572554	4.6674031828889263E-4
50-51	0.0	0.0	0.0	0.16354580752842798	4.6674031828889263E-4
52-53	0.0	0.0	0.0	0.19332383983525936	4.900773342033373E-4
54-55	0.0	0.0	0.0	0.22382531963543847	5.134143501177819E-4
56-57	0.0	2.333701591444463E-5	0.0	0.25894752858667763	5.134143501177819E-4
58-59	0.0	4.667403182888926E-5	0.0	0.29927389208683797	5.134143501177819E-4
60-61	0.0	4.667403182888926E-5	0.0	0.3425873936240472	5.134143501177819E-4
62-63	0.0	4.667403182888926E-5	0.0	0.38926142545293646	5.134143501177819E-4
64-65	0.0	4.667403182888926E-5	0.0	0.43889925830296017	5.134143501177819E-4
66-67	0.0	4.667403182888926E-5	0.0	0.49476807440214066	5.367513660322265E-4
68-69	0.0	4.667403182888926E-5	0.0	0.5557710340024988	5.600883819466712E-4
70-71	0.0	4.667403182888926E-5	0.0	0.624428534822795	6.067624137755604E-4
72-73	0.0	4.667403182888926E-5	0.0	0.6972400244758623	6.534364456044497E-4
74-75	0.0	4.667403182888926E-5	0.0	0.776212486330343	6.534364456044497E-4
76-77	0.0	4.667403182888926E-5	0.0	0.8595256331449103	6.767734615188944E-4
78-79	0.0	4.667403182888926E-5	0.0	0.955090713314561	7.00110477433339E-4
80-81	0.0	4.667403182888926E-5	0.0	1.0598272407385885	7.467845092622282E-4
82-83	0.0	4.667403182888926E-5	0.0	1.1753221324991752	7.934585410911175E-4
84-85	0.0	4.667403182888926E-5	0.0	1.2941075435036984	7.934585410911175E-4
86-87	0.0	4.667403182888926E-5	0.0	1.4249581917359893	8.401325729200067E-4
88-89	0.0	4.667403182888926E-5	0.0	1.570324463867065	8.401325729200067E-4
90-91	0.0	4.667403182888926E-5	0.0	1.734453696793354	8.401325729200067E-4
92-93	0.0	4.667403182888926E-5	0.0	1.9152455590825566	8.86806604748896E-4
94-95	0.0	4.667403182888926E-5	0.0	2.1067257746605748	9.801546684066746E-4
96-97	0.0	4.667403182888926E-5	0.0	2.3088710065114944	0.0010034916843211193
98-99	0.0	4.667403182888926E-5	0.0	2.5247384037201073	0.0010268287002355638
100-101	0.0	4.667403182888926E-5	0.0	2.7741410927977768	0.0010268287002355638
102-103	0.0	4.667403182888926E-5	0.0	3.039879693015558	0.001213524827551121
104-105	0.0	4.667403182888926E-5	0.0	3.3147197294399726	0.0012601988593800102
106-107	0.0	4.667403182888926E-5	0.0	3.6008782185828925	0.0012601988593800102
108-109	0.0	4.667403182888926E-5	0.0	3.910793789926717	0.0012601988593800102
110-111	0.0	4.667403182888926E-5	0.0	4.245819990394484	0.0013068728912088994
112-113	0.0	4.667403182888926E-5	0.0	4.602923007917316	0.0013535469230377887
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2180	0.0	73.160645	1
GTATCAA	4650	0.0	58.615402	1
TCAACGC	5950	0.0	45.0959	4
CAACGCA	6185	0.0	43.670033	5
ATCAACG	6390	0.0	41.89565	3
AACGCAG	6535	0.0	41.239162	6
GTACATG	11120	0.0	39.495792	1
TATCAAC	6940	0.0	38.74683	2
TACATGG	11805	0.0	36.637486	2
ACATGGG	11965	0.0	36.097836	3
ACGCAGA	7505	0.0	35.748074	7
CATGGGG	9865	0.0	34.255375	4
CGCAGAG	7940	0.0	33.863716	8
GCAGAGT	8860	0.0	29.67599	9
ATGGGGG	5185	0.0	29.258955	5
AGAGTAC	7865	0.0	26.81168	10-11
CAGAGTA	8215	0.0	25.379728	10-11
ATGGGGA	3855	0.0	24.846706	5
GTATAGA	665	0.0	22.372658	1
GTATAGG	615	0.0	21.288584	1
>>END_MODULE