FastQCFastQC Report
Mon 15 Aug 2016
SRR2921542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2142519
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21820.10184273745063638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26900.079.110581
GTATCAA57650.062.970431
TCAACGC77150.045.9178964
CAACGCA78900.044.877425
AACGCAG82900.042.846616
ATCAACG83200.042.6504753
TATCAAC86600.041.4572372
GTACATG109600.036.8754921
ACGCAGA96700.036.668767
ACATGGG115250.034.4576643
CGCAGAG103050.034.40848
TACATGG117200.034.3415572
GCAGAGT110550.031.4267989
CATGGGG94250.030.2590374
AGAGTAC103500.027.93456610-11
ATGGGGG49300.027.521745
CAGAGTA106200.026.18804710-11
GAGTACT51200.024.2260212-13
GTATAGG6550.022.7518831
AGTACTT57250.022.44524212-13
Produced by FastQC (version 0.11.5)