##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921542_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2142519
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12123626441586	31.0	31.0	34.0	28.0	34.0
2	31.194830010842377	33.0	31.0	34.0	27.0	34.0
3	31.273629778779092	33.0	31.0	34.0	27.0	34.0
4	34.78331254005215	37.0	35.0	37.0	32.0	37.0
5	34.41744600631313	37.0	35.0	37.0	30.0	37.0
6	34.388076838525116	37.0	35.0	37.0	30.0	37.0
7	34.22824021630613	37.0	35.0	37.0	30.0	37.0
8	34.19716697961605	37.0	35.0	37.0	30.0	37.0
9	35.7653523726044	39.0	37.0	39.0	30.0	39.0
10-11	35.618223922401626	39.0	35.0	39.0	30.0	39.0
12-13	35.52737151922574	39.0	35.0	39.0	30.0	39.0
14-15	36.68072488505352	40.0	37.0	41.0	30.0	41.0
16-17	36.65451648270097	40.0	37.0	41.0	30.0	41.0
18-19	36.665441706701316	40.0	37.0	41.0	29.5	41.0
20-21	36.63832969509255	40.0	37.0	41.0	29.0	41.0
22-23	36.567515153891286	40.0	36.5	41.0	29.0	41.0
24-25	36.49199937083405	40.0	36.0	41.0	29.0	41.0
26-27	36.36596641616714	40.0	36.0	41.0	28.5	41.0
28-29	36.18050738406521	40.0	36.0	41.0	27.5	41.0
30-31	36.14062955801092	40.0	36.0	41.0	28.0	41.0
32-33	36.026389964336374	40.0	35.0	41.0	28.0	41.0
34-35	35.90312034572389	40.0	35.0	41.0	27.0	41.0
36-37	35.77156351939003	40.0	35.0	41.0	27.0	41.0
38-39	35.60303969299689	40.0	35.0	41.0	26.5	41.0
40-41	35.406790558216755	39.0	35.0	41.0	25.5	41.0
42-43	35.24628276342007	39.0	35.0	41.0	25.0	41.0
44-45	35.03593690417681	39.0	35.0	41.0	24.0	41.0
46-47	34.78622499963828	39.0	34.5	41.0	23.5	41.0
48-49	34.55125858860528	39.0	34.0	41.0	23.0	41.0
50-51	34.34342402564458	39.0	34.0	41.0	22.0	41.0
52-53	34.14739775936643	38.0	34.0	40.5	21.5	41.0
54-55	33.92841090324053	38.0	33.0	40.0	20.5	41.0
56-57	33.670126379275985	37.5	33.0	40.0	20.0	41.0
58-59	33.524669092782844	37.0	33.0	40.0	20.0	41.0
60-61	33.40743792703822	37.0	33.0	40.0	20.0	41.0
62-63	33.142124293880244	36.0	33.0	40.0	19.0	41.0
64-65	32.84858547345438	36.0	33.0	39.0	18.0	41.0
66-67	32.548467948242234	35.0	32.5	39.0	18.0	41.0
68-69	32.2336457693024	35.0	32.0	39.0	17.0	40.5
70-71	31.933320544648613	35.0	32.0	37.5	14.5	40.0
72-73	31.621979315002577	35.0	32.0	37.0	13.0	39.0
74-75	31.334638339263268	35.0	32.0	36.5	11.5	39.0
76-77	31.04715454098657	35.0	31.5	36.0	9.5	39.0
78-79	30.772180783460964	35.0	31.0	36.0	7.5	37.0
80-81	30.54954915218955	35.0	31.0	35.0	7.0	37.0
82-83	30.364754524930703	35.0	31.0	35.0	7.0	36.5
84-85	30.17314105499181	35.0	31.0	35.0	7.0	36.0
86-87	30.036173541518185	35.0	31.0	35.0	3.5	36.0
88-89	29.928519653734693	35.0	31.0	35.0	2.0	36.0
90-91	29.828933372352825	35.0	31.0	35.0	2.0	35.0
92-93	29.892223826253115	35.0	31.0	35.0	2.0	35.0
94-95	29.862432725217374	35.0	31.0	35.0	2.0	35.0
96-97	29.844980837976234	35.0	31.0	35.0	2.0	35.0
98-99	29.803317030094014	35.0	31.0	35.0	2.0	35.0
100-101	29.755527022164095	35.0	31.0	35.0	2.0	35.0
102-103	28.864968291996476	33.5	29.5	35.0	2.0	35.0
104-105	29.581695658241536	34.5	31.0	35.0	2.0	35.0
106-107	29.693278566024386	35.0	31.0	35.0	2.0	35.0
108-109	29.69850955814161	35.0	31.0	35.0	2.0	35.0
110-111	29.664613476006515	35.0	31.0	35.0	2.0	35.0
112-113	29.62009041693446	35.0	31.0	35.0	2.0	35.0
114-115	29.557285139595027	35.0	31.0	35.0	2.0	35.0
116-117	29.483490694831644	35.0	31.0	35.0	2.0	35.0
118-119	29.388298073435987	35.0	31.0	35.0	2.0	35.0
120-121	29.263765922262536	35.0	31.0	35.0	2.0	35.0
122-123	29.145199645837447	35.0	31.0	35.0	2.0	35.0
124-125	28.23029013978406	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41445.0
3	15088.0
4	8325.0
5	6977.0
6	7274.0
7	8301.0
8	9406.0
9	10378.0
10	11797.0
11	12196.0
12	12804.0
13	13912.0
14	17424.0
15	14023.0
16	8326.0
17	7097.0
18	6476.0
19	6013.0
20	5782.0
21	6376.0
22	7513.0
23	10493.0
24	14284.0
25	19957.0
26	24620.0
27	27521.0
28	31995.0
29	38361.0
30	45075.0
31	54308.0
32	67516.0
33	87102.0
34	121666.0
35	193255.0
36	381132.0
37	601502.0
38	186558.0
39	241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.040647613973846	18.105479109565835	12.89059305420227	24.96328022225805
2	16.833108827782304	19.944167663172596	37.7464776899359	25.476245819109206
3	18.483189970281693	25.023784915275282	29.22328130062172	27.2697438138213
4	13.303161884409509	16.15803911422778	35.691662798332615	34.8471362030301
5	14.378364852221948	37.290172343925946	33.34375974558309	14.987703058269016
6	33.03568177425725	35.54611311692907	17.061512476996892	14.356692631816786
7	29.407917647355386	31.434944043558914	20.975146134678244	18.181992174407455
8	27.750137578643873	33.384163916260704	19.39697845091348	19.468720054181947
9	27.902464388701098	14.31733380382536	18.967363349604515	38.81283845786903
10-11	26.104653211696053	24.89060650057261	27.074345570266345	21.93039471746499
12-13	26.736212566898587	22.69592545259524	27.211072328420826	23.35678965208535
14-15	24.38965818618019	23.682482931479708	24.62361739442955	27.304241487910552
16-17	24.106627307154962	26.519451263430057	25.16523106208599	24.20869036732899
18-19	24.190864493724355	25.489798871364496	26.998613710727636	23.320722924183514
20-21	25.388071475917958	24.480968696610123	26.663850326290927	23.467109501180996
22-23	25.205442109552635	24.18207083779304	26.40453319305	24.207953859604327
24-25	24.589298826328008	24.73888447245444	26.465036076538183	24.206780624679368
26-27	24.191831847811628	24.76396192023227	26.66015511040472	24.384051121551384
28-29	24.424175883680306	24.61194715423245	26.74376375316609	24.220113208921155
30-31	24.694404264253567	24.614342137910864	26.44673779513572	24.244515802699844
32-33	24.26895323079178	24.78434508334331	26.446566664496057	24.500135021368852
34-35	24.59076699283619	24.743095108437497	26.520007478689582	24.146130420036727
36-37	24.74890674772854	24.551589183617832	26.233433166902557	24.46607090175107
38-39	24.682651852457006	24.88647172442769	26.152876574836466	24.27799984827884
40-41	24.612060746478047	24.739376379787554	26.382389878978962	24.266172994755436
42-43	24.63516055876115	24.781332058096112	26.090247305933005	24.493260077209733
44-45	24.634814971284925	24.769701715668827	25.974229372819817	24.621253940226428
46-47	24.789356870622324	24.921044566785525	25.672331976232726	24.617266586359424
48-49	24.917306286869632	25.16869610845288	25.285917991831813	24.628079612845667
50-51	24.73661582671751	25.29692425543118	25.245038658620782	24.721421259230524
52-53	24.976722309819113	25.158578899640418	25.320804968085813	24.543893822454656
54-55	24.851850581079027	25.096554745554585	25.42967357762203	24.621921095744355
56-57	24.7583843913224	25.13794351825938	25.27925407446604	24.824418015952173
58-59	24.735769653598112	24.965980678425797	25.48027346166145	24.81797620631464
60-61	24.83987230398318	25.172533639012034	25.499452994780636	24.488141062224148
62-63	24.674893911029987	25.26103255262433	25.507126284388466	24.55694725195722
64-65	24.778879419202234	24.902563164137828	25.57214527226529	24.746412144394643
66-67	24.770517794571504	25.11884014538794	25.381995767570686	24.728646292469875
68-69	24.779864142860777	25.109994983729216	25.285062600678472	24.825078272731538
70-71	24.651656629529022	24.92065440799249	25.699685978645427	24.728002983833058
72-73	24.984851653573887	25.17594489269649	25.22806547446768	24.611137979261937
74-75	24.710546192635583	25.3383768926185	25.18922683786012	24.761850076885803
76-77	24.719465122369176	25.123013359337182	25.323106999332946	24.8344145189607
78-79	24.690003233084372	25.169176683975923	25.24751770317951	24.89330237976019
80-81	24.689918337031422	25.339398729375933	25.139407785921925	24.831275147670716
82-83	24.820697307180463	25.209993619088028	25.14511090043677	24.82419817329474
84-85	24.59616726210635	25.20011044439398	25.14339449397819	25.060327799521488
86-87	24.4155693810637	25.29433923863441	25.318029217796568	24.97206216250532
88-89	24.642716509931955	25.14785130396482	25.22910328945664	24.980328896646586
90-91	24.549856016476067	25.09015016391575	25.326932924543673	25.03306089506451
92-93	24.59670380041319	25.2897991267517	25.197283725411303	24.916213347423806
94-95	24.448590932959917	25.0275745376043	25.38684870762588	25.13698582180991
96-97	24.58595666961809	25.071681317209254	25.27658509878089	25.06577691439176
98-99	24.463403227281976	25.072369268568632	25.130176001314364	25.334051502835024
100-101	24.41533465047047	25.169253678045163	25.292510639986276	25.122901031498095
102-103	24.564629839971232	24.877588060632213	25.427096954270063	25.130685145126495
104-105	24.33016627521853	25.195101732951468	25.242570264628895	25.232161727201103
106-107	24.40569247022599	25.099526747660285	25.233341477968995	25.261439304144734
108-109	24.436034767605197	25.04681134340036	25.143725179250914	25.37342870974353
110-111	24.451260978397695	25.14103830893119	25.17441049090713	25.233290221763983
112-113	24.356980667584825	25.02597050087183	25.393023358397116	25.224025473146234
114-115	24.119232907300376	25.334694062345537	25.31609411340788	25.229978916946212
116-117	24.265721290680943	25.278437422325638	25.20298316224066	25.252858124752752
118-119	24.19372719606836	24.919625367272687	25.301899373643842	25.584748063015113
120-121	23.96115916648152	25.060515226592532	25.45954698296705	25.518778623958895
122-123	23.982458854831414	25.16380938111944	25.345465857613902	25.508265906435245
124-125	23.977406336505812	25.23435276992497	25.301322375434548	25.486918518134665
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	17.5
2	33.5
3	64.0
4	56.0
5	39.5
6	60.0
7	128.0
8	231.0
9	286.0
10	278.0
11	284.5
12	323.5
13	391.0
14	492.5
15	619.5
16	743.5
17	859.5
18	1010.5
19	1179.5
20	1357.5
21	1577.0
22	1805.0
23	2088.0
24	2461.0
25	3110.5
26	4383.0
27	5796.5
28	6814.0
29	8649.5
30	11720.0
31	14663.0
32	18304.0
33	22405.5
34	26684.0
35	31202.5
36	36154.5
37	41184.5
38	43886.0
39	46547.0
40	48903.0
41	50393.5
42	52865.0
43	58187.0
44	63674.5
45	67009.5
46	71065.5
47	74962.5
48	80019.5
49	85287.5
50	86377.0
51	85549.5
52	84896.5
53	85750.0
54	87513.0
55	87143.5
56	83202.0
57	76967.5
58	72567.0
59	68096.0
60	61115.5
61	53811.5
62	46413.0
63	36565.0
64	27565.0
65	21872.5
66	17089.5
67	13127.5
68	10673.5
69	8854.5
70	7655.0
71	6473.5
72	5287.0
73	4900.5
74	4110.0
75	3144.5
76	2239.0
77	1351.0
78	801.5
79	507.0
80	301.0
81	144.5
82	75.5
83	36.5
84	21.5
85	18.0
86	13.0
87	5.5
88	2.0
89	2.0
90	2.0
91	3.5
92	3.5
93	1.5
94	1.5
95	0.5
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.124339620792161
2	0.001540243050353346
3	0.018902982890700155
4	0.05605551222649601
5	0.02441051864650909
6	0.019463071272646824
7	0.014795668089757897
8	0.005040795437520041
9	0.0037805965781400304
10-11	0.006464353408301164
12-13	0.004527381087402259
14-15	0.002403712639187797
16-17	0.010081590875040082
18-19	0.00508746946934893
20-21	0.012088574243682319
22-23	0.008774717983831181
24-25	0.012508640530142324
26-27	0.008121281538226733
28-29	0.01330209907123344
30-31	0.020186518765994605
32-33	0.012391955450570098
34-35	0.020186518765994605
36-37	0.01339544713489122
38-39	0.02013984473416572
40-41	0.009591513540836745
42-43	0.008051270490483398
44-45	0.016405922187854576
46-47	0.015379093487619015
48-49	0.01995314860685016
50-51	0.014282253739640116
52-53	0.020839955211599058
54-55	0.015892507837736793
56-57	0.01999982263867905
58-59	0.0165226072674268
60-61	0.01869294974747015
62-63	0.02095664029117128
64-65	0.016895999522057916
66-67	0.01169184497313676
68-69	0.022846938580241297
70-71	0.014375601803297893
72-73	0.017316065808517918
74-75	0.01778280612680681
76-77	0.012695336657457881
78-79	0.028074430145076896
80-81	0.01969644143179127
82-83	0.008961414111146739
84-85	0.01234528141874121
86-87	0.012275270370997878
88-89	0.01948640828856127
90-91	0.012135248275511209
92-93	0.008074607506397843
94-95	0.006721060583360055
96-97	0.0023337015914444634
98-99	0.0016335911140111243
100-101	0.011505148845821203
102-103	0.0028237789256478005
104-105	0.0021936794959577953
106-107	2.100331432300017E-4
108-109	0.0191363530498446
110-111	7.467845092622282E-4
112-113	0.008611358872430069
114-115	0.0018669612731555707
116-117	0.0075378561403656175
118-119	0.009684861604494523
120-121	0.0038739446417978087
122-123	6.300994296900051E-4
124-125	0.023453700994016856
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2142519.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.83444355287968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.31993917807155	42.70144788299742
2	10.572760193752389	11.383573236973925
3	3.5725472488801056	5.769782796294949
4	1.7107678785723328	3.683929471643279
5	1.0643626904669399	2.8649686589866805
6	0.7191751208389203	2.322983546846297
7	0.5095665478368014	1.9202562089169235
8	0.3718959449766282	1.601664900591131
9	0.29176764730180244	1.413643404530285
>10	1.7011672834194094	16.769025227192277
>50	0.11435742231890782	4.207702804648384
>100	0.050560302974095424	4.808589546853067
>500	7.840665623658567E-4	0.25110133344220203
>1k	3.4847402771813636E-4	0.30133098008331355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2182	0.10184273745063638	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.667403182888926E-5
2	0.0	0.0	0.0	0.0	4.667403182888926E-5
3	0.0	0.0	0.0	0.0	4.667403182888926E-5
4	0.0	0.0	0.0	0.0	4.667403182888926E-5
5	0.0	0.0	0.0	0.0	4.667403182888926E-5
6	0.0	0.0	0.0	0.0	9.334806365777853E-5
7	0.0	0.0	0.0	0.0	9.334806365777853E-5
8	0.0	0.0	0.0	0.0	9.334806365777853E-5
9	0.0	0.0	0.0	0.0	9.334806365777853E-5
10-11	0.0	0.0	0.0	0.0	9.334806365777853E-5
12-13	0.0	0.0	0.0	0.0	1.8669612731555705E-4
14-15	0.0	0.0	0.0	2.333701591444463E-5	2.3337015914444632E-4
16-17	0.0	0.0	0.0	4.667403182888926E-5	3.2671822280222484E-4
18-19	0.0	0.0	0.0	4.667403182888926E-5	3.2671822280222484E-4
20-21	0.0	0.0	0.0	7.00110477433339E-5	3.2671822280222484E-4
22-23	0.0	0.0	0.0	1.400220954866678E-4	3.2671822280222484E-4
24-25	0.0	0.0	0.0	2.3337015914444632E-4	3.733922546311141E-4
26-27	0.0	0.0	0.0	3.733922546311141E-4	3.733922546311141E-4
28-29	0.0	0.0	0.0	0.001143513779807787	3.733922546311141E-4
30-31	0.0	0.0	0.0	0.0031738341643644703	3.733922546311141E-4
32-33	0.0	0.0	0.0	0.007561193156280061	3.733922546311141E-4
34-35	0.0	0.0	0.0	0.013628817294035665	3.733922546311141E-4
36-37	0.0	0.0	0.0	0.0223801982619524	3.733922546311141E-4
38-39	0.0	0.0	0.0	0.03712919231988141	3.733922546311141E-4
40-41	0.0	0.0	0.0	0.05584547908326601	3.733922546311141E-4
42-43	0.0	0.0	0.0	0.07227473828703503	3.9672927054555874E-4
44-45	0.0	0.0	0.0	0.09208786479839852	4.43403302374448E-4
46-47	4.667403182888926E-5	0.0	0.0	0.1141413448375487	5.134143501177819E-4
48-49	4.667403182888926E-5	0.0	0.0	0.13908861485009	6.534364456044497E-4
50-51	4.667403182888926E-5	0.0	0.0	0.16688300080419355	6.767734615188944E-4
52-53	4.667403182888926E-5	0.0	0.0	0.1969177402860838	8.16795557005562E-4
54-55	4.667403182888926E-5	0.0	0.0	0.2275592421817496	8.86806604748896E-4
56-57	4.667403182888926E-5	0.0	0.0	0.2633115505626788	9.101436206633406E-4
58-59	4.667403182888926E-5	0.0	0.0	0.30475809082673244	9.5681765249223E-4
60-61	4.667403182888926E-5	0.0	0.0	0.34867835477771725	9.801546684066746E-4
62-63	4.667403182888926E-5	0.0	0.0	0.396215856195441	0.0010268287002355638
64-65	4.667403182888926E-5	0.0	0.0	0.44639044041149695	0.0010735027320644532
66-67	4.667403182888926E-5	0.0	0.0	0.502819344892624	0.0010735027320644532
68-69	4.667403182888926E-5	0.0	0.0	0.564849133193218	0.0010735027320644532
70-71	4.667403182888926E-5	0.0	0.0	0.6342300815068618	0.0010968397479788976
72-73	4.667403182888926E-5	0.0	0.0	0.7081850849397369	0.0011668507957222317
74-75	4.667403182888926E-5	0.0	0.0	0.7885811047649987	0.0011668507957222317
76-77	4.667403182888926E-5	0.0	0.0	0.8730611023752881	0.0011901878116366764
78-79	4.667403182888926E-5	0.0	0.0	0.9701430885793778	0.0012368618434655655
80-81	4.667403182888926E-5	0.0	0.0	1.0761864888946142	0.0013068728912088994
82-83	4.667403182888926E-5	0.0	0.0	1.1939917452307307	0.0013535469230377887
84-85	4.667403182888926E-5	0.0	0.0	1.3136639628400029	0.0013535469230377887
86-87	7.00110477433339E-5	0.0	0.0	1.4464049093613638	0.0014002209548666781
88-89	9.334806365777853E-5	0.0	0.0	1.5939881980043118	0.0014468949866955673
90-91	9.334806365777853E-5	0.0	0.0	1.7608945358244197	0.0014468949866955673
92-93	9.334806365777853E-5	0.0	0.0	1.94451017703927	0.0014935690185244564
94-95	9.334806365777853E-5	0.0	0.0	2.141124536118466	0.001586917082182235
96-97	9.334806365777853E-5	0.0	0.0	2.3476804639772157	0.0016102540980966796
98-99	9.334806365777853E-5	0.0	0.0	2.566675021318364	0.0016335911140111243
100-101	9.334806365777853E-5	0.0	0.0	2.8193682296399705	0.0016335911140111243
102-103	9.334806365777853E-5	0.0	0.0	3.0900309402156996	0.0016802651458400135
104-105	9.334806365777853E-5	0.0	0.0	3.3688616063614836	0.0017269391776689028
106-107	9.334806365777853E-5	0.0	0.0	3.660224250053325	0.0017269391776689028
108-109	9.334806365777853E-5	0.0	0.0	3.976720859885023	0.0017736132094977922
110-111	9.334806365777853E-5	0.0	0.0	4.320941844623082	0.00191363530498446
112-113	9.334806365777853E-5	0.0	0.0	4.686446187875113	0.0019603093368133493
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2690	0.0	79.11058	1
GTATCAA	5765	0.0	62.97043	1
TCAACGC	7715	0.0	45.917896	4
CAACGCA	7890	0.0	44.87742	5
AACGCAG	8290	0.0	42.84661	6
ATCAACG	8320	0.0	42.650475	3
TATCAAC	8660	0.0	41.457237	2
GTACATG	10960	0.0	36.875492	1
ACGCAGA	9670	0.0	36.66876	7
ACATGGG	11525	0.0	34.457664	3
CGCAGAG	10305	0.0	34.4084	8
TACATGG	11720	0.0	34.341557	2
GCAGAGT	11055	0.0	31.426798	9
CATGGGG	9425	0.0	30.259037	4
AGAGTAC	10350	0.0	27.934566	10-11
ATGGGGG	4930	0.0	27.52174	5
CAGAGTA	10620	0.0	26.188047	10-11
GAGTACT	5120	0.0	24.22602	12-13
GTATAGG	655	0.0	22.751883	1
AGTACTT	5725	0.0	22.445242	12-13
>>END_MODULE