FastQCFastQC Report
Mon 15 Aug 2016
SRR2921541_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921541_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2134988
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23830.1116165524115358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24950.073.712081
GTATCAA49500.059.5183371
TCAACGC65800.044.306584
CAACGCA67050.043.479565
AACGCAG71050.041.0269286
ATCAACG73100.039.8801163
TATCAAC75700.038.902442
GTACATG116900.038.1854171
TACATGG122500.035.9873352
ACATGGG123600.035.090273
ACGCAGA82850.034.895597
CATGGGG98800.032.5790444
CGCAGAG89400.032.137048
GCAGAGT96300.028.9689439
ATGGGGG53400.027.5196915
AGAGTAC87650.025.89071310-11
CAGAGTA92750.024.72360610-11
CATGGGT22200.024.6565844
ATGGGTA16500.022.7166615
ATGGGGA40200.022.4960045
Produced by FastQC (version 0.11.5)