##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921541_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2134988
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.962664895540396	31.0	30.0	34.0	26.0	34.0
2	30.047299094889528	31.0	30.0	34.0	25.0	34.0
3	30.161873509359303	31.0	30.0	34.0	26.0	34.0
4	33.537675621596	37.0	35.0	37.0	27.0	37.0
5	33.57991005101668	37.0	35.0	37.0	28.0	37.0
6	33.58001824834613	37.0	35.0	37.0	28.0	37.0
7	33.4345059550686	37.0	35.0	37.0	27.0	37.0
8	33.356400598036146	37.0	35.0	37.0	27.0	37.0
9	34.828805126773545	39.0	35.0	39.0	27.0	39.0
10-11	34.70725526326143	39.0	35.0	39.0	26.5	39.0
12-13	34.550222062138054	39.0	35.0	39.0	25.5	39.0
14-15	35.70535220806862	40.0	36.0	41.0	25.5	41.0
16-17	35.63635509895138	40.0	36.0	41.0	25.0	41.0
18-19	35.65653038799282	40.0	36.0	41.0	25.0	41.0
20-21	35.68214505186914	40.0	36.0	41.0	25.0	41.0
22-23	35.65132567489841	40.0	36.0	41.0	25.0	41.0
24-25	35.61493647739472	40.0	35.5	41.0	25.0	41.0
26-27	35.541221777358935	40.0	35.0	41.0	25.0	41.0
28-29	35.49566133392787	40.0	35.0	41.0	25.0	41.0
30-31	35.39001296494406	40.0	35.0	41.0	25.0	41.0
32-33	35.26904015385567	40.0	35.0	41.0	25.0	41.0
34-35	35.0906543737014	40.0	35.0	41.0	24.0	41.0
36-37	34.939530339280594	39.0	35.0	41.0	24.0	41.0
38-39	34.80088881061627	39.0	34.5	41.0	23.0	41.0
40-41	34.63666540514514	39.0	34.0	41.0	22.5	41.0
42-43	34.42488576048203	39.0	34.0	41.0	21.5	41.0
44-45	34.21832675406138	39.0	33.5	41.0	19.5	41.0
46-47	33.94230014407575	38.5	33.0	41.0	18.0	41.0
48-49	33.77312144143199	38.0	33.0	41.0	15.5	41.0
50-51	33.560408302060715	38.0	33.0	40.0	14.0	41.0
52-53	33.354558667308666	38.0	33.0	40.0	13.0	41.0
54-55	33.13794784795043	37.5	33.0	40.0	10.5	41.0
56-57	32.88178622081249	37.0	32.0	40.0	8.5	41.0
58-59	32.62577307226083	36.5	32.0	40.0	8.0	41.0
60-61	32.31071579793423	36.0	32.0	40.0	7.0	41.0
62-63	31.99924964449449	35.5	31.0	39.0	4.0	41.0
64-65	31.684283471382507	35.0	31.0	39.0	2.0	41.0
66-67	31.633752274017464	35.0	31.0	39.0	2.0	41.0
68-69	31.3903993839778	35.0	31.0	37.5	2.0	40.0
70-71	31.093540104206674	35.0	31.0	37.0	2.0	39.5
72-73	30.78070438803403	35.0	31.0	37.0	2.0	39.0
74-75	30.486017485812567	35.0	31.0	36.0	2.0	39.0
76-77	30.22251881509404	35.0	31.0	36.0	2.0	38.0
78-79	29.962490889878538	35.0	30.5	35.0	2.0	37.0
80-81	29.738872302795144	35.0	30.0	35.0	2.0	37.0
82-83	29.5581865097134	35.0	30.0	35.0	2.0	36.0
84-85	29.370951499493206	35.0	30.0	35.0	2.0	36.0
86-87	29.231575072084713	35.0	30.0	35.0	2.0	36.0
88-89	29.11521399651895	34.0	30.0	35.0	2.0	35.0
90-91	29.014726312279038	34.0	30.0	35.0	2.0	35.0
92-93	28.918540994141416	34.0	30.0	35.0	2.0	35.0
94-95	28.854393560994254	34.0	30.0	35.0	2.0	35.0
96-97	28.763382276621694	34.0	29.0	35.0	2.0	35.0
98-99	28.70549600278784	34.0	29.0	35.0	2.0	35.0
100-101	28.64205091550866	34.0	29.0	35.0	2.0	35.0
102-103	27.671867242345158	32.5	27.5	34.5	2.0	35.0
104-105	28.488408834148014	34.0	29.0	35.0	2.0	35.0
106-107	28.815283270913	34.5	30.0	35.0	2.0	35.0
108-109	28.858158219156266	35.0	30.0	35.0	2.0	35.0
110-111	28.802065632219012	35.0	30.0	35.0	2.0	35.0
112-113	28.727830320357775	35.0	30.0	35.0	2.0	35.0
114-115	28.610839498863694	35.0	30.0	35.0	2.0	35.0
116-117	28.54934383706138	35.0	30.0	35.0	2.0	35.0
118-119	28.395477398467815	35.0	30.0	35.0	2.0	35.0
120-121	28.227032657794798	34.5	29.5	35.0	2.0	35.0
122-123	28.10260783667168	34.5	29.0	35.0	2.0	35.0
124-125	27.256453666250117	34.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77765.0
3	14009.0
4	9254.0
5	7935.0
6	8535.0
7	9715.0
8	11046.0
9	11617.0
10	12360.0
11	13314.0
12	13672.0
13	14241.0
14	16135.0
15	13719.0
16	9369.0
17	8174.0
18	7634.0
19	6972.0
20	6752.0
21	7072.0
22	8416.0
23	11470.0
24	16342.0
25	22922.0
26	27355.0
27	29087.0
28	34032.0
29	40571.0
30	45662.0
31	55317.0
32	68904.0
33	89787.0
34	126210.0
35	201972.0
36	384757.0
37	546866.0
38	145884.0
39	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.24104064199537	16.61322796467657	12.315266319654807	24.830465073673253
2	16.93344996366625	18.86722086377349	38.26625917889934	25.93306999366092
3	19.352659349679584	23.743039007793314	28.51540062425213	28.388901018274975
4	13.633386247650247	14.726501076715765	34.8278660411907	36.812246634443284
5	14.960901461562356	36.66575610889771	32.698223194108586	15.675119235431348
6	34.23815450595508	33.70319066381253	17.0379610642294	15.02069376600299
7	30.4431680484918	30.878567137771107	20.13420900468293	18.544055809054164
8	28.33482099975869	32.879255370241516	19.199424612796744	19.586499017203046
9	28.0993350209642	14.499944479661986	18.54711943150922	38.853601067864595
10-11	26.944479195805098	24.398125956736198	26.229389905112388	22.428004942346323
12-13	27.094547485530935	22.0665230380544	26.567818132065852	24.271111344348817
14-15	24.530637995908172	23.17119311272068	24.02774553257146	28.27042335879969
16-17	24.768154730946097	26.18764472758181	24.376622987167917	24.66757755430418
18-19	24.873692226583096	25.216823853318495	25.987721867494578	23.92176205260383
20-21	26.044823705003527	24.157121294247748	25.63554381966282	24.162511181085904
22-23	25.766039525565738	23.67400441516208	25.625327030600808	24.93462902867137
24-25	25.171898699053774	24.207953601646853	25.627459545803095	24.99268815349628
26-27	24.809605226808955	24.265278736823262	25.74867444749022	25.17644158887757
28-29	24.87774794731364	24.381288629188376	25.696947998299834	25.044015425198147
30-31	25.23345675326994	24.282103203185518	25.54204564678779	24.942394396756747
32-33	24.848118582561955	24.327318690166592	25.472398894521675	25.352163832749774
34-35	25.223265013309913	24.35562523809546	25.623523105275563	24.797586643319057
36-37	25.274591121372147	24.250786546130488	25.449743644658056	25.02487868783931
38-39	25.31384797738509	24.310045314654115	25.351319214870948	25.02478749308985
40-41	25.341575059818982	24.278915319939003	25.44755283424354	24.93195678599847
42-43	25.215858341249977	24.339630246366507	25.408669765209673	25.03584164717384
44-45	25.14858645150269	24.35182140989989	25.297038159939316	25.202553978658106
46-47	25.23024897354656	24.399665348056768	25.256988319991002	25.113097358405668
48-49	25.534957458334663	24.592906535801916	24.760111494966043	25.112024510897378
50-51	25.298489294453436	24.937970696909677	24.729077982942265	25.034462025694626
52-53	25.484226407100287	24.60130733045221	24.911244101101346	25.003222161346155
54-55	25.306380474125895	24.636050175543172	25.084256973459524	24.973312376871416
56-57	25.18135513835849	24.66228382474537	24.854106377988604	25.302254658907543
58-59	25.333408935469592	24.486071438864073	24.869088418650893	25.31143120701544
60-61	25.210412212552995	24.639348571380378	25.127573312192546	25.022665903874074
62-63	25.147460470050675	24.81904682020753	24.934929955837422	25.09856275390438
64-65	25.209284083257227	24.528211705288648	25.034546810049008	25.227957401405117
66-67	25.129122417375942	24.650398390040365	25.03871446878595	25.181764723797745
68-69	25.215494370264484	24.707983019547292	24.910387841842184	25.166134768346044
70-71	25.103530753683266	24.500271098974792	25.07890456331977	25.31729358402217
72-73	25.30085200698023	24.768901100986334	24.914451414939744	25.01579547709369
74-75	25.07011109533563	24.92236821574115	24.852854558310643	25.15466613061258
76-77	25.186255359483035	24.610305669162877	24.882924198601792	25.320514772752293
78-79	25.190497408729218	24.632792026916796	24.93223713278617	25.244473431567823
80-81	25.12007076228039	24.85562578634842	24.81088401081205	25.21341944055915
82-83	25.22338682249637	24.796546299653617	24.78642748793941	25.19363938991061
84-85	25.048421887981654	24.818498437780715	24.753194181550047	25.37988549268759
86-87	24.836249041994822	24.933150445694928	24.81280250945825	25.417798002852
88-89	24.952494952003036	24.753034654323475	24.81700718197619	25.477463211697298
90-91	24.949789143680928	24.68666669008864	24.868023033169028	25.495521133061406
92-93	25.04371441213514	24.906732827585206	24.77153144146649	25.27802131881316
94-95	24.91718973026174	24.649868670009216	24.89746798026326	25.535473619465787
96-97	24.910340621319822	24.720599078297838	24.87366178458702	25.49539851579532
98-99	24.91171800727804	24.685160544759093	24.755636240481156	25.64748520748171
100-101	24.80279579225864	24.85319912431237	24.84959219441633	25.494412889012658
102-103	24.973154109347618	24.60324876848502	25.039293952490326	25.384303169677036
104-105	24.71492623025304	24.80151164980238	24.925031125783764	25.55853099416081
106-107	24.794590650979178	24.81147730080604	24.860614875475594	25.533317172739185
108-109	24.810691497758746	24.67978581028255	24.876132630485262	25.633390061473442
110-111	24.897440677664413	24.83879520081392	24.89964222511806	25.364121896403606
112-113	24.864539504578197	24.837909967808006	24.919531722991923	25.378018804621878
114-115	24.539190905888614	25.05986419540273	25.061011826691747	25.33993307201691
116-117	24.63417924479251	25.115640063948074	24.869979581770583	25.38020110948883
118-119	24.779081602950647	24.722883681100033	24.917886244514012	25.580148471435304
120-121	24.406449682740266	24.74722275818907	25.1711914672302	25.67513609184046
122-123	24.460367613436183	24.84421968351062	25.07163551997361	25.62377718307959
124-125	24.429914102147645	24.964079441871228	25.10073564834646	25.505270807634666
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	98.0
1	131.5
2	184.0
3	199.0
4	186.5
5	179.0
6	195.5
7	240.5
8	333.5
9	408.0
10	428.0
11	482.5
12	597.0
13	726.5
14	828.0
15	926.5
16	1026.5
17	1121.5
18	1251.0
19	1391.5
20	1540.0
21	1745.5
22	1960.5
23	2265.5
24	2730.5
25	3381.0
26	4245.0
27	5256.5
28	6313.0
29	7634.0
30	9455.0
31	11307.5
32	13307.5
33	15223.5
34	17150.5
35	19765.0
36	23007.0
37	26503.0
38	30102.5
39	34574.0
40	39000.5
41	42864.5
42	47477.0
43	54383.0
44	60532.0
45	65164.5
46	71953.0
47	78080.0
48	84496.0
49	91787.0
50	93247.0
51	92369.5
52	91839.5
53	92575.0
54	94022.5
55	93088.0
56	89115.0
57	82145.5
58	77625.0
59	73048.0
60	65327.5
61	58591.5
62	51858.0
63	40939.0
64	30920.0
65	24904.0
66	19482.5
67	14903.0
68	12110.5
69	10147.0
70	8874.0
71	7501.5
72	6090.0
73	5636.0
74	4678.0
75	3526.5
76	2480.5
77	1457.5
78	892.0
79	576.5
80	328.0
81	152.5
82	105.0
83	66.5
84	42.5
85	28.5
86	21.5
87	17.5
88	12.5
89	13.0
90	10.5
91	10.0
92	13.5
93	13.0
94	11.0
95	10.5
96	9.0
97	6.0
98	6.0
99	6.5
100	23.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03592526046984808
2	0.02852475049040088
3	0.02782217042906096
4	0.033723842944316317
5	0.035831583128336085
6	0.04740073480506682
7	0.04000022482561963
8	0.03620629249438404
9	0.029742555930056747
10-11	0.03648732451892001
12-13	0.036534163189676006
14-15	0.034403003670278244
16-17	0.037751968629331875
18-19	0.052131440551422305
20-21	0.06381768890504302
22-23	0.056417178925595834
24-25	0.0685718139867765
26-27	0.049953442361268544
28-29	0.050445248404206486
30-31	0.05063260308723047
32-33	0.058595177115749596
34-35	0.04051545020393557
36-37	0.053091633301920196
38-39	0.06330246352672708
40-41	0.04918060429379463
42-43	0.060749755970525364
44-45	0.06077317530590336
46-47	0.06688562183956069
48-49	0.05058576441647447
50-51	0.05234221456982428
52-53	0.062154916093205205
54-55	0.048712217586234674
56-57	0.04611267135927696
58-59	0.04728363812817683
60-61	0.033958036298096295
62-63	0.04409860851676918
64-65	0.04341944779080725
66-67	0.03620629249438404
68-69	0.030515393997530664
70-71	0.05063260308723047
72-73	0.03159268342491855
74-75	0.04098383691149552
76-77	0.03217816680936848
78-79	0.03311494022448838
80-81	0.02423901211622735
82-83	0.01639353476459821
84-85	0.017072695490560133
86-87	0.016112502740062238
88-89	0.022388884621365554
90-91	0.01126470031681677
92-93	0.01847785561323998
94-95	0.013536375848482522
96-97	0.011334958322950762
98-99	0.009812701523380929
100-101	0.009765862852624933
102-103	0.0055035438138293985
104-105	0.004777544417111478
106-107	0.0077518000101171524
108-109	0.011475474335218746
110-111	0.005901672515255355
112-113	0.006580833241217281
114-115	0.007306832637935201
116-117	0.00613586586903533
118-119	0.23494277251207032
120-121	0.35000196722417176
122-123	0.026791719672429073
124-125	0.003208448946785649
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2134988.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.067136412131475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.59950331639112	43.282495710494906
2	11.006808240501481	12.122268216837357
3	3.8394617342915836	6.342844892141805
4	1.8512447349178724	4.077709853998506
5	1.1000864159030954	3.0289304364834275
6	0.7229364031525193	2.388602251781727
7	0.5046864314356833	1.9454145595654428
8	0.368007810202392	1.6212109028115929
9	0.28283867205680835	1.4017604163100545
>10	1.599003388497577	15.775439855466775
>50	0.08670471207853475	3.2627987121692903
>100	0.035972669173134714	3.467575626520683
>500	0.002144899529786938	0.7794071988108205
>1k	6.005718683403318E-4	0.5035413666076018
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2383	0.1116165524115358	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.6838670755994883E-5
2	0.0	0.0	0.0	0.0	4.6838670755994883E-5
3	0.0	0.0	0.0	0.0	4.6838670755994883E-5
4	0.0	0.0	0.0	0.0	4.6838670755994883E-5
5	0.0	0.0	0.0	0.0	4.6838670755994883E-5
6	0.0	0.0	0.0	0.0	9.367734151198977E-5
7	0.0	0.0	0.0	0.0	9.367734151198977E-5
8	0.0	0.0	0.0	0.0	9.367734151198977E-5
9	0.0	0.0	0.0	0.0	1.4051601226798465E-4
10-11	0.0	0.0	0.0	0.0	1.4051601226798465E-4
12-13	0.0	0.0	0.0	0.0	2.10774018401977E-4
14-15	0.0	0.0	0.0	0.0	3.7470936604795907E-4
16-17	0.0	0.0	0.0	0.0	4.918060429379463E-4
18-19	0.0	0.0	0.0	0.0	6.323220552059309E-4
20-21	0.0	0.0	0.0	0.0	6.557413905839283E-4
22-23	0.0	0.0	0.0	7.025800613399233E-5	7.025800613399232E-4
24-25	0.0	0.0	0.0	1.4051601226798465E-4	7.494187320959181E-4
26-27	0.0	0.0	0.0	1.6393534764598208E-4	8.196767382299104E-4
28-29	0.0	0.0	0.0	7.49418732095918E-4	8.430960736079078E-4
30-31	0.0	0.0	0.0	0.002880578251493685	8.430960736079078E-4
32-33	0.0	0.0	0.0	0.007119477954911222	8.430960736079078E-4
34-35	0.0	0.0	0.0	0.013489537177726527	8.430960736079078E-4
36-37	0.0	0.0	0.0	0.022857271328925503	8.430960736079078E-4
38-39	0.0	0.0	0.0	0.03789248464159986	8.899347443639028E-4
40-41	0.0	0.0	0.0	0.05601905022416988	9.836120858758926E-4
42-43	0.0	0.0	0.0	0.07309174571473001	0.0010772894273878824
44-45	0.0	0.0	0.0	0.09173353667561598	0.001170966768899872
46-47	0.0	0.0	0.0	0.11468448534605347	0.0013114827811678566
48-49	0.0	0.0	0.0	0.13676891860750506	0.0013114827811678566
50-51	0.0	0.0	0.0	0.16386508963984808	0.0013583214519238516
52-53	0.0	0.0	0.0	0.19159358272739707	0.0014051601226798464
54-55	0.0	0.0	0.0	0.22243684742021969	0.0014988374641918363
56-57	0.0	0.0	0.0	0.25616069036453604	0.0014988374641918363
58-59	0.0	0.0	0.0	0.2950602064273897	0.0014988374641918363
60-61	0.0	0.0	0.0	0.3407044910791068	0.0014988374641918363
62-63	0.0	0.0	0.0	0.38417077754067	0.0014988374641918363
64-65	0.0	0.0	0.0	0.429674546180119	0.0014988374641918363
66-67	0.0	0.0	0.0	0.4840308235924511	0.0014988374641918363
68-69	0.0	0.0	0.0	0.5475440611375801	0.0014988374641918363
70-71	0.0	0.0	0.0	0.6187856793574484	0.0015222567995698336
72-73	0.0	0.0	0.0	0.6904722649494985	0.0016627728118378182
74-75	0.0	0.0	0.0	0.7643134293963245	0.0017330308179718107
76-77	0.0	0.0	0.0	0.8469368446098995	0.0017330308179718107
78-79	0.0	0.0	0.0	0.9432371516842248	0.0017330308179718107
80-81	0.0	0.0	0.0	1.0487412575621033	0.0017564501533498082
82-83	0.0	0.0	0.0	1.1633789042374008	0.0020140628425077797
84-85	0.0	0.0	0.0	1.2806395164750342	0.0020140628425077797
86-87	0.0	0.0	0.0	1.408836958334192	0.0020609015132637748
88-89	0.0	0.0	0.0	1.5542241923608002	0.0021077401840197698
90-91	0.0	0.0	0.0	1.7149510910599965	0.0021077401840197698
92-93	0.0	0.0	0.0	1.8850223045750139	0.0021545788547757644
94-95	0.0	0.0	0.0	2.067763378529528	0.0022482561962877544
96-97	0.0	0.0	0.0	2.2583030911649153	0.0023653528731777413
98-99	0.0	0.0	0.0	2.464182468472891	0.0024824495500677286
100-101	0.0	0.0	0.0	2.700296207753861	0.0024824495500677286
102-103	0.0	0.0	0.0	2.964653665500696	0.0025527075562017214
104-105	0.0	0.0	0.0	3.2416341450162713	0.0026229655623357133
106-107	0.0	0.0	0.0	3.523275072272069	0.002786900909981695
108-109	0.0	0.0	0.0	3.8134874762762134	0.0029274169222496802
110-111	0.0	0.0	0.0	4.13758765857232	0.003278706952919642
112-113	0.0	0.0	0.0	4.485177434252558	0.0033255456236756364
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2495	0.0	73.71208	1
GTATCAA	4950	0.0	59.518337	1
TCAACGC	6580	0.0	44.30658	4
CAACGCA	6705	0.0	43.47956	5
AACGCAG	7105	0.0	41.026928	6
ATCAACG	7310	0.0	39.880116	3
TATCAAC	7570	0.0	38.90244	2
GTACATG	11690	0.0	38.185417	1
TACATGG	12250	0.0	35.987335	2
ACATGGG	12360	0.0	35.09027	3
ACGCAGA	8285	0.0	34.89559	7
CATGGGG	9880	0.0	32.579044	4
CGCAGAG	8940	0.0	32.13704	8
GCAGAGT	9630	0.0	28.968943	9
ATGGGGG	5340	0.0	27.519691	5
AGAGTAC	8765	0.0	25.890713	10-11
CAGAGTA	9275	0.0	24.723606	10-11
CATGGGT	2220	0.0	24.656584	4
ATGGGTA	1650	0.0	22.716661	5
ATGGGGA	4020	0.0	22.496004	5
>>END_MODULE