FastQCFastQC Report
Mon 15 Aug 2016
SRR2921541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2134988
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37930.1776590781774886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33830.1584552231675307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29400.082.9369051
GTATCAA65500.065.5334551
TCAACGC90250.046.9714664
CAACGCA91400.046.292545
ATCAACG94650.044.913713
AACGCAG95800.044.1560256
TATCAAC97700.043.7563822
ACGCAGA112750.037.514427
GTACATG116550.036.0107541
CGCAGAG120750.035.0757948
TACATGG121300.033.9178852
ACATGGG121950.033.3945543
GCAGAGT127500.032.704869
CATGGGG101750.032.0076834
AGAGTAC118300.028.28442210-11
ATGGGGG54200.027.9980965
CAGAGTA123500.026.7804210-11
GAGTACT69250.024.48216812-13
AGTACTT71600.024.13558812-13
GTGGTAT11500.023.3284851
Produced by FastQC (version 0.11.5)