##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921541_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2134988
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34236398518399	31.0	31.0	34.0	28.0	34.0
2	31.39270478335241	33.0	31.0	34.0	28.0	34.0
3	31.47069772757505	33.0	31.0	34.0	28.0	34.0
4	34.98400834103049	37.0	35.0	37.0	32.0	37.0
5	34.65808051380148	37.0	35.0	37.0	32.0	37.0
6	34.63907197604858	37.0	35.0	37.0	31.0	37.0
7	34.49294703295756	37.0	35.0	37.0	30.0	37.0
8	34.456293899544164	37.0	35.0	37.0	30.0	37.0
9	36.028983301077105	39.0	37.0	39.0	30.0	39.0
10-11	35.90989504390657	39.0	37.0	39.0	30.0	39.0
12-13	35.812219319265495	39.0	37.0	39.0	30.0	39.0
14-15	37.005030707432546	40.0	37.0	41.0	30.0	41.0
16-17	37.00146886071491	40.0	37.0	41.0	30.0	41.0
18-19	37.02616665761119	40.0	37.0	41.0	30.0	41.0
20-21	37.014614133662576	40.0	37.0	41.0	30.0	41.0
22-23	36.95921148034556	40.0	37.0	41.0	30.0	41.0
24-25	36.89861886811542	40.0	37.0	41.0	30.0	41.0
26-27	36.78054232623322	40.0	36.5	41.0	30.0	41.0
28-29	36.58635271017917	40.0	36.0	41.0	30.0	41.0
30-31	36.52771139697272	40.0	36.0	41.0	30.0	41.0
32-33	36.392518365442804	40.0	35.5	41.0	30.0	41.0
34-35	36.25904407893627	40.0	35.0	41.0	29.0	41.0
36-37	36.11124441917238	40.0	35.0	41.0	29.0	41.0
38-39	35.92985229893564	40.0	35.0	41.0	28.0	41.0
40-41	35.70849484868299	39.5	35.0	41.0	27.0	41.0
42-43	35.52107342055318	39.0	35.0	41.0	26.5	41.0
44-45	35.283803702877954	39.0	35.0	41.0	25.5	41.0
46-47	34.98160177012704	39.0	35.0	41.0	24.5	41.0
48-49	34.698676058132406	39.0	34.0	41.0	23.0	41.0
50-51	34.45822739987297	38.5	34.0	41.0	23.0	41.0
52-53	34.242339067011144	38.0	34.0	40.5	22.5	41.0
54-55	34.013779936936416	38.0	33.5	40.0	22.0	41.0
56-57	33.74531988001806	37.5	33.0	40.0	21.5	41.0
58-59	33.584830921766304	37.0	33.0	40.0	21.5	41.0
60-61	33.4547873336993	37.0	33.0	40.0	21.0	41.0
62-63	33.18581088043586	36.0	33.0	39.5	20.0	41.0
64-65	32.8859131760928	35.5	33.0	39.0	20.0	41.0
66-67	32.57954658293161	35.0	33.0	39.0	19.5	41.0
68-69	32.26509961648496	35.0	32.5	38.5	18.5	40.5
70-71	31.96620285453595	35.0	32.0	37.0	18.0	40.0
72-73	31.667961599784167	35.0	32.0	37.0	17.5	39.0
74-75	31.389791183838035	35.0	32.0	36.0	16.5	39.0
76-77	31.1153936228213	35.0	32.0	36.0	14.0	38.5
78-79	30.858461499549414	35.0	32.0	35.0	12.5	37.0
80-81	30.652403901099213	35.0	31.0	35.0	11.0	37.0
82-83	30.483968996547052	35.0	31.0	35.0	10.0	36.0
84-85	30.30971415296011	35.0	31.0	35.0	7.5	36.0
86-87	30.179447378626953	35.0	31.0	35.0	7.0	36.0
88-89	30.08447190335496	35.0	31.0	35.0	7.0	35.5
90-91	29.993051248999997	35.0	31.0	35.0	7.0	35.0
92-93	30.062387704286863	35.0	31.0	35.0	6.0	35.0
94-95	30.04057048564207	35.0	31.0	35.0	3.5	35.0
96-97	30.0286144465449	35.0	31.0	35.0	2.0	35.0
98-99	29.988352861936463	35.0	31.0	35.0	2.0	35.0
100-101	29.94078772339704	35.0	31.0	35.0	2.0	35.0
102-103	29.055079232295448	33.5	29.5	35.0	2.0	35.0
104-105	29.759890922103544	34.5	31.0	35.0	2.0	35.0
106-107	29.871873518727035	35.0	31.0	35.0	2.0	35.0
108-109	29.87955997879145	35.0	31.0	35.0	2.0	35.0
110-111	29.848380178249243	35.0	31.0	35.0	2.0	35.0
112-113	29.803923253901196	35.0	31.0	35.0	2.0	35.0
114-115	29.740057789551976	35.0	31.0	35.0	2.0	35.0
116-117	29.6666222479939	35.0	31.0	35.0	2.0	35.0
118-119	29.57629363724761	35.0	31.0	35.0	2.0	35.0
120-121	29.468185769662405	35.0	31.0	35.0	2.0	35.0
122-123	29.347886264466123	35.0	31.0	35.0	2.0	35.0
124-125	28.432368706522006	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31604.0
3	11579.0
4	6788.0
5	5694.0
6	6364.0
7	7553.0
8	8872.0
9	9949.0
10	11468.0
11	12657.0
12	13420.0
13	15352.0
14	19981.0
15	16568.0
16	8777.0
17	7396.0
18	6444.0
19	6071.0
20	5776.0
21	6023.0
22	7133.0
23	9962.0
24	14241.0
25	19951.0
26	24647.0
27	26973.0
28	32147.0
29	38376.0
30	44563.0
31	53233.0
32	66805.0
33	86742.0
34	121199.0
35	196231.0
36	393861.0
37	604601.0
38	175655.0
39	332.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.247714424391354	17.188700967691258	12.615673925328094	24.947910682589296
2	17.083389735876843	19.40756485746043	37.34778924668529	26.16125615997744
3	19.391860679754018	23.933017815128572	28.742156161834416	27.932965343282994
4	13.73247852432972	15.214662973741804	34.55490413860653	36.497954363321945
5	14.815307633201522	36.76305777844412	32.64271256957589	15.778922018778463
6	34.273828012452476	34.17666308271434	16.416609862193518	15.132899042639664
7	30.015398518050745	31.26915738203644	19.93431152415347	18.781132575759347
8	28.443264253437327	32.997263066480365	18.741975510464098	19.81749716961821
9	28.084888324927782	14.195144594662324	18.425789697779614	39.29417738263028
10-11	27.00520863219712	24.43009872253957	26.013205319507975	22.55148732575534
12-13	27.327776256741902	22.06060728131453	26.622653171109977	23.98896329083359
14-15	24.673003046249264	22.97654643346204	24.384655072636193	27.965795447652503
16-17	24.59265588410408	26.02149298533828	24.698312630607056	24.687538499950577
18-19	24.67108460400753	24.623587288612942	26.952243885130507	23.753084222249026
20-21	25.749355466814883	23.632532114230465	26.783700354783107	23.834412064171545
22-23	25.430875579274876	23.175843330065238	26.759794095975188	24.6334869946847
24-25	24.90619106198745	23.78139730111334	26.693924741106066	24.618486895793144
26-27	24.548861094865703	23.833147171515346	26.818497263081664	24.799494470537287
28-29	24.702147907967483	23.940691215948593	26.735845569677775	24.621315306406153
30-31	24.95510046322595	23.875450195890114	26.633723551906492	24.535725788977448
32-33	24.601012841932526	23.870179532217026	26.580015379307454	24.948792246542993
34-35	24.875403842405888	23.97758065604246	26.638396136605554	24.508619364946107
36-37	25.026602840150368	23.861254389205115	26.412691036265823	24.699451734378695
38-39	25.117117345032536	23.978632980160032	26.235433094659673	24.668816580147755
40-41	25.09649832861147	23.945919713390595	26.317225607471034	24.640356350526897
42-43	25.100906016933333	24.032530699248895	26.139676458994554	24.726886824823218
44-45	25.019137307959337	24.017766542645045	25.95023644062	25.012859708775615
46-47	25.095550263408832	24.221245150459602	25.72449433959701	24.958710246534554
48-49	25.51339129897029	24.448355878215004	24.982161956782978	25.056090866031727
50-51	25.25311698414085	24.800791516865527	24.927347081035613	25.018744417958004
52-53	25.532028031262033	24.563944091143767	25.0103772639067	24.893650613687495
54-55	25.261691314809127	24.59443936407043	25.192637788488202	24.951231532632242
56-57	25.164089797058047	24.534598702648207	24.997516985226532	25.303794515067214
58-59	25.239963777638174	24.40045947869219	25.16538281536574	25.1941939283039
60-61	25.251643403418615	24.533776587085097	25.288513545326495	24.92606646416979
62-63	25.075404570468017	24.6999746074699	25.171961774403766	25.05265904765831
64-65	25.225359647857104	24.29604682496107	25.302892174040142	25.175701353141683
66-67	25.16045377230906	24.5262977420532	25.227183616453065	25.086064869184675
68-69	25.301531928370707	24.570846416427024	25.032233369595875	25.095388285606397
70-71	25.043238938467667	24.396762935375822	25.331529758976885	25.22846836717963
72-73	25.394679330510293	24.695783387934213	24.988744863746092	24.9207924178094
74-75	25.186256445716836	24.81887342432177	24.863964747720487	25.130905382240904
76-77	25.256759795379168	24.527844922892424	24.90799557779152	25.307399703936888
78-79	25.191034978763476	24.559585249461765	24.962856587304007	25.28652318447075
80-81	25.183222194815315	24.802851817417533	24.825925152910624	25.18800083485652
82-83	25.253977864255496	24.72563364183471	24.795102077143692	25.225286416766114
84-85	25.08089557461681	24.732485390780376	24.738879742389607	25.4477392922132
86-87	24.864981976516773	24.798579669885676	24.838280519882044	25.498157833715506
88-89	24.93347494113469	24.682459965500666	24.778054601144795	25.60601049221985
90-91	24.976905571425494	24.649976530813962	24.86693767877102	25.50618021898952
92-93	25.003413655135816	24.858576307898343	24.738588964771314	25.399421072194535
94-95	24.862505650372515	24.58376955380209	24.92164426425709	25.632080531568306
96-97	24.92455871679676	24.65937543399962	24.81265853793915	25.60340731126447
98-99	24.90042485862051	24.615640071374187	24.731099693177182	25.75283537682812
100-101	24.810016468167202	24.764507082764077	24.789615709193388	25.635860739875334
102-103	24.96127491368533	24.50875231448845	24.982516917487814	25.547455854338402
104-105	24.842014837478395	24.748405276703256	24.734985642396488	25.67459424342186
106-107	24.879542366379496	24.737035376862483	24.67267889252514	25.710743364232876
108-109	24.91905124759344	24.555926727646536	24.68616620196629	25.838855822793732
110-111	24.92242859789266	24.7151890063015	24.75069304301211	25.611689352793732
112-113	24.907496626127106	24.704338184558928	24.740102876774124	25.648062312539842
114-115	24.597763117957637	24.899528604136144	24.85390662764598	25.648801650260246
116-117	24.704979669155648	24.9371782647687	24.66448427155478	25.69335779452087
118-119	24.719061648691625	24.620081222035864	24.765062017350385	25.895795111922133
120-121	24.38373360719568	24.670235152355446	24.92399500209027	26.022036238358602
122-123	24.4259477450678	24.70719219026167	24.842908033370875	26.023952031299658
124-125	24.463697664580128	24.892022729689756	24.723667671284346	25.92061193444577
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	38.0
2	74.0
3	122.0
4	101.0
5	92.0
6	173.0
7	366.5
8	580.5
9	685.5
10	626.5
11	549.0
12	605.0
13	716.0
14	904.0
15	1121.0
16	1298.0
17	1505.0
18	1718.5
19	1908.0
20	2157.0
21	2430.0
22	2816.0
23	3182.0
24	3528.5
25	4168.5
26	5010.0
27	5914.5
28	6956.5
29	8280.5
30	9936.0
31	11654.0
32	13629.5
33	15503.5
34	17215.0
35	19691.5
36	22910.5
37	26201.0
38	29476.5
39	33798.0
40	38485.5
41	42300.0
42	46429.5
43	53337.5
44	60095.0
45	64468.5
46	70877.0
47	77678.5
48	84625.0
49	91738.0
50	92842.0
51	92547.5
52	92499.0
53	92959.0
54	94535.0
55	93511.5
56	89295.0
57	82297.0
58	77843.5
59	73345.5
60	65413.5
61	58409.5
62	51239.5
63	40334.0
64	30512.5
65	24457.5
66	18948.0
67	14397.5
68	11667.0
69	9584.0
70	8428.0
71	7227.5
72	5954.5
73	5584.0
74	4578.0
75	3365.0
76	2279.5
77	1313.5
78	839.5
79	565.0
80	291.0
81	94.5
82	53.0
83	33.0
84	19.5
85	12.5
86	4.5
87	3.0
88	3.5
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12145267327029471
2	0.0017330308179718105
3	0.02388772208555739
4	0.05517595415056197
5	0.029414685234764787
6	0.02215469126758558
7	0.017189792167450122
8	0.005480124478451401
9	0.004168641697283544
10-11	0.007798638680873149
12-13	0.005269350460049424
14-15	0.0031616102760296545
16-17	0.011475474335218746
18-19	0.0061124465336573315
20-21	0.014215536574444446
22-23	0.009695604846490942
24-25	0.015456761349478312
26-27	0.009203798803552994
28-29	0.015433342014100314
30-31	0.023442754713375438
32-33	0.014121859232932457
34-35	0.02304462601194948
36-37	0.01627643808770822
38-39	0.022646497310523525
40-41	0.011053926298414792
42-43	0.009578508169600953
44-45	0.019391209692981883
46-47	0.017728436881144064
48-49	0.023653528731777416
50-51	0.016510631441488195
52-53	0.02407507676858137
54-55	0.01929753235146989
56-57	0.022763593987413514
58-59	0.01850127494861798
60-61	0.021990755919939597
62-63	0.02351301271950943
64-65	0.018735468302397952
66-67	0.012224893067314663
68-69	0.025948623598821163
70-71	0.01583147071552627
72-73	0.019063338997689916
74-75	0.020187467095833793
76-77	0.014426310592846424
78-79	0.032459198833904454
80-81	0.022927529335059496
82-83	0.009625346840356949
84-85	0.013653472525372507
86-87	0.012786957116386603
88-89	0.021264756523221677
90-91	0.012833795787142596
92-93	0.008407541400701081
94-95	0.008103090040787115
96-97	0.0023185142024217467
98-99	0.0019906435071297825
100-101	0.01234198974420465
102-103	0.003138190940651657
104-105	0.0026463848977137105
106-107	2.341933537799744E-4
108-109	0.021475530541623652
110-111	9.133540797419002E-4
112-113	0.00941457282195497
114-115	0.002435610879311734
116-117	0.008313864059189092
118-119	0.01042160424320886
120-121	0.004473093057197511
122-123	5.85483384449936E-4
124-125	0.023934560756313384
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2134988.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.62257825781788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.04774428484933	41.85121275760492
2	11.207415874718201	12.019410696199744
3	3.8625067946850278	6.213527186080537
4	1.9318197699405852	4.143566271745551
5	1.1371336515999744	3.048801911125892
6	0.7590403832387927	2.4421021410639745
7	0.5225370990734333	1.9613850541374818
8	0.40454473729276513	1.735418546741574
9	0.3040138961028181	1.4671808041713732
>10	1.6824373903554088	16.170259617766625
>50	0.09705868381896282	3.5261009069539737
>100	0.04068084630213487	3.8978651037581904
>500	0.0025408872189244926	0.9009342626409338
>1k	5.257008039154037E-4	0.6222347400091977
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3793	0.1776590781774886	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3383	0.1584552231675307	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.6838670755994883E-5
2	0.0	0.0	0.0	0.0	4.6838670755994883E-5
3	0.0	0.0	0.0	0.0	4.6838670755994883E-5
4	0.0	0.0	0.0	0.0	4.6838670755994883E-5
5	0.0	0.0	0.0	0.0	4.6838670755994883E-5
6	0.0	0.0	0.0	0.0	4.6838670755994883E-5
7	0.0	0.0	0.0	0.0	4.6838670755994883E-5
8	0.0	0.0	0.0	0.0	4.6838670755994883E-5
9	0.0	0.0	0.0	0.0	4.6838670755994883E-5
10-11	0.0	0.0	0.0	0.0	4.6838670755994883E-5
12-13	0.0	0.0	0.0	0.0	3.0445135991396676E-4
14-15	0.0	0.0	0.0	0.0	3.7470936604795907E-4
16-17	0.0	0.0	0.0	0.0	4.6838670755994883E-4
18-19	0.0	0.0	0.0	0.0	6.089027198279335E-4
20-21	0.0	0.0	0.0	0.0	6.323220552059309E-4
22-23	0.0	0.0	0.0	2.3419335377997442E-5	7.025800613399232E-4
24-25	0.0	0.0	0.0	9.367734151198977E-5	7.494187320959181E-4
26-27	0.0	0.0	0.0	1.4051601226798465E-4	7.494187320959181E-4
28-29	0.0	0.0	0.0	7.49418732095918E-4	7.494187320959181E-4
30-31	0.0	0.0	0.0	0.00302109426376167	7.728380674739155E-4
32-33	0.0	0.0	0.0	0.0077518000101171524	7.962574028519129E-4
34-35	0.0	0.0	0.0	0.014402891257468426	8.430960736079078E-4
36-37	0.0	0.0	0.0	0.02414533477471536	8.430960736079078E-4
38-39	0.0	0.0	0.0	0.039812870142595644	9.367734151198977E-4
40-41	0.0	0.0	0.0	0.05843124176810361	9.367734151198977E-4
42-43	0.0	0.0	0.0	0.07622993665538166	9.601927504978951E-4
44-45	0.0	0.0	0.0	0.09566798501911955	0.00100703142125389
46-47	0.0	0.0	0.0	0.11925125574476297	0.0010538700920098847
48-49	0.0	0.0	0.0	0.14192117239066449	0.0010772894273878822
50-51	0.0	0.0	0.0	0.16969650414896947	0.0011241280981438772
52-53	0.0	0.0	0.0	0.19779970660256638	0.0011943861042778695
54-55	0.0	0.0	0.0	0.22983735739966688	0.0012646441104118618
56-57	0.0	0.0	0.0	0.26482584445439505	0.0012880634457898593
58-59	0.0	0.0	0.0	0.30520077864606265	0.001381740787301849
60-61	0.0	0.0	0.0	0.35234390076197153	0.0014519987934358413
62-63	0.0	0.0	0.0	0.3971685086754586	0.0014988374641918363
64-65	0.0	0.0	0.0	0.44417111477909943	0.0015222567995698336
66-67	0.0	0.0	0.0	0.5001667456678913	0.0015690954703258286
68-69	0.0	0.0	0.0	0.5650148853295662	0.0015925148057038259
70-71	0.0	0.0	0.0	0.6379426956966503	0.0016159341410818234
72-73	0.0	0.0	0.0	0.7112217960944043	0.0018267081594838005
74-75	0.0	0.0	0.0	0.7868194106945801	0.0019672241717517847
76-77	0.0	0.0	0.0	0.8712226953968828	0.0020609015132637748
78-79	0.0	0.0	0.0	0.9688813239231321	0.002131159519397767
80-81	0.0	0.0	0.0	1.0769849760279684	0.0022014175255317594
82-83	0.0	0.0	0.0	1.1940816529179554	0.0022482561962877544
84-85	0.0	0.0	0.0	1.3130050379674265	0.002295094867043749
86-87	0.0	0.0	0.0	1.4442001547549683	0.0023185142024217467
88-89	0.0	0.0	0.0	1.59305345041752	0.002341933537799744
90-91	0.0	0.0	0.0	1.758440796856938	0.002341933537799744
92-93	0.0	0.0	0.0	1.9328680067522628	0.002341933537799744
94-95	0.0	0.0	0.0	2.1198245610748163	0.002341933537799744
96-97	0.0	0.0	0.0	2.316055172207057	0.002599546226957716
98-99	0.0	0.0	0.0	2.5269462872859236	0.002763481574603698
100-101	0.0	0.0	0.0	2.7678375709840055	0.002763481574603698
102-103	0.0	0.0	0.0	3.036269993086612	0.0028337395807376906
104-105	0.0	0.0	0.0	3.318496403726859	0.002880578251493685
106-107	0.0	0.0	0.0	3.6059453261564	0.0029039975868716825
108-109	0.0	0.0	0.0	3.9036753368168817	0.0029508362576276775
110-111	0.0	0.0	0.0	4.237377446617966	0.00302109426376167
112-113	0.0	0.0	0.0	4.595107794516878	0.003044513599139667
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2940	0.0	82.936905	1
GTATCAA	6550	0.0	65.533455	1
TCAACGC	9025	0.0	46.971466	4
CAACGCA	9140	0.0	46.29254	5
ATCAACG	9465	0.0	44.91371	3
AACGCAG	9580	0.0	44.156025	6
TATCAAC	9770	0.0	43.756382	2
ACGCAGA	11275	0.0	37.51442	7
GTACATG	11655	0.0	36.010754	1
CGCAGAG	12075	0.0	35.075794	8
TACATGG	12130	0.0	33.917885	2
ACATGGG	12195	0.0	33.394554	3
GCAGAGT	12750	0.0	32.70486	9
CATGGGG	10175	0.0	32.007683	4
AGAGTAC	11830	0.0	28.284422	10-11
ATGGGGG	5420	0.0	27.998096	5
CAGAGTA	12350	0.0	26.78042	10-11
GAGTACT	6925	0.0	24.482168	12-13
AGTACTT	7160	0.0	24.135588	12-13
GTGGTAT	1150	0.0	23.328485	1
>>END_MODULE