FastQCFastQC Report
Mon 15 Aug 2016
SRR2921540_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921540_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1828159
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20150.075.020041
GTATCAA39550.059.4386941
TCAACGC51250.045.1651084
CAACGCA52400.044.2862365
ATCAACG55400.041.7795143
AACGCAG56050.041.61126
TATCAAC60450.038.387682
GTACATG98450.037.902731
TACATGG103350.036.040222
ACGCAGA66900.034.59397
ACATGGG107150.034.3733633
CGCAGAG71150.032.4403348
CATGGGG88950.032.310864
ATGGGGG46450.030.487845
GCAGAGT76000.029.9004869
GTGGTAT8250.024.5269551
CAGAGTA76650.024.44707310-11
AGAGTAC72650.024.2782610-11
GTATATA4150.022.9451311
GGGTACC12850.021.2976027
Produced by FastQC (version 0.11.5)