FastQCFastQC Report
Mon 15 Aug 2016
SRR2921540_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921540_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1828159
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34730.1899725352116528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29810.16306021522198016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29550.083.522331
GTATCAA60600.061.287961
TCAACGC80650.045.4841584
CAACGCA82750.044.3751145
ATCAACG85100.043.2456863
AACGCAG85600.042.8152586
TATCAAC89450.041.2091982
ACGCAGA98950.036.9745947
CGCAGAG104350.034.9471788
GTACATG96900.034.2066651
TACATGG99950.032.649882
GCAGAGT110850.032.1993989
GTGGTAT10800.032.015771
ACATGGG101100.031.8661753
CATGGGG81950.029.7933084
CAGAGTA109650.027.34387210-11
AGAGTAC105000.027.22339410-11
GTATACG1107.6655904E-427.097991
GAGTACT61300.026.1064512-13
ATGGGGG41750.025.5163695
Produced by FastQC (version 0.11.5)