##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921540_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1828159
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.318440026277802	31.0	31.0	34.0	28.0	34.0
2	31.372154172585645	33.0	31.0	34.0	28.0	34.0
3	31.45184253667214	33.0	31.0	34.0	28.0	34.0
4	34.97794229057757	37.0	35.0	37.0	32.0	37.0
5	34.64221492769502	37.0	35.0	37.0	31.0	37.0
6	34.6294529086365	37.0	35.0	37.0	31.0	37.0
7	34.47499260184699	37.0	35.0	37.0	30.0	37.0
8	34.44111699255918	37.0	35.0	37.0	30.0	37.0
9	36.02140404636577	39.0	37.0	39.0	30.0	39.0
10-11	35.90228284301311	39.0	37.0	39.0	30.0	39.0
12-13	35.808251634567895	39.0	37.0	39.0	30.0	39.0
14-15	36.98765834919173	40.0	37.0	41.0	30.0	41.0
16-17	36.97282183880068	40.0	37.0	41.0	30.0	41.0
18-19	36.99978913212691	40.0	37.0	41.0	30.0	41.0
20-21	36.98639396245075	40.0	37.0	41.0	30.0	41.0
22-23	36.92041501860615	40.0	37.0	41.0	30.0	41.0
24-25	36.852491769041976	40.0	37.0	41.0	30.0	41.0
26-27	36.723270514216765	40.0	36.5	41.0	30.0	41.0
28-29	36.52850627325085	40.0	36.0	41.0	29.5	41.0
30-31	36.466678773564006	40.0	36.0	41.0	30.0	41.0
32-33	36.32759048857348	40.0	35.5	41.0	29.5	41.0
34-35	36.19195649831333	40.0	35.0	41.0	29.0	41.0
36-37	36.04440204599271	40.0	35.0	41.0	28.5	41.0
38-39	35.85791006143339	40.0	35.0	41.0	27.5	41.0
40-41	35.643783719031006	39.0	35.0	41.0	27.0	41.0
42-43	35.45126900887723	39.0	35.0	41.0	26.0	41.0
44-45	35.210693927606954	39.0	35.0	41.0	25.5	41.0
46-47	34.89721162108985	39.0	34.5	41.0	24.0	41.0
48-49	34.60969778886847	39.0	34.0	41.0	23.0	41.0
50-51	34.36731104898425	38.5	34.0	41.0	22.5	41.0
52-53	34.163797569029825	38.0	34.0	40.5	22.0	41.0
54-55	33.94003694427016	38.0	33.0	40.0	21.5	41.0
56-57	33.6678757154055	37.5	33.0	40.0	20.0	41.0
58-59	33.51703352935932	37.0	33.0	40.0	20.5	41.0
60-61	33.39510376285651	37.0	33.0	40.0	20.0	41.0
62-63	33.12729937603895	36.0	33.0	40.0	20.0	41.0
64-65	32.83660283377978	36.0	33.0	39.0	19.0	41.0
66-67	32.53879914165015	35.0	33.0	39.0	18.0	41.0
68-69	32.227520418081795	35.0	32.0	38.5	18.0	40.5
70-71	31.93040922589337	35.0	32.0	37.0	17.0	40.0
72-73	31.632108585741175	35.0	32.0	37.0	14.5	39.0
74-75	31.349439244617123	35.0	32.0	36.5	13.0	39.0
76-77	31.075538834423043	35.0	32.0	36.0	11.5	38.5
78-79	30.80798551985905	35.0	31.0	35.5	10.0	37.0
80-81	30.597401538925226	35.0	31.0	35.0	7.5	37.0
82-83	30.41802709720544	35.0	31.0	35.0	7.0	36.5
84-85	30.234558372657958	35.0	31.0	35.0	7.0	36.0
86-87	30.10167195522928	35.0	31.0	35.0	7.0	36.0
88-89	30.00112107316705	35.0	31.0	35.0	5.0	35.5
90-91	29.909041554919458	35.0	31.0	35.0	2.0	35.0
92-93	29.97521714467943	35.0	31.0	35.0	2.0	35.0
94-95	29.94961980878031	35.0	31.0	35.0	2.0	35.0
96-97	29.934156711752095	35.0	31.0	35.0	2.0	35.0
98-99	29.89802145218222	35.0	31.0	35.0	2.0	35.0
100-101	29.84581401289494	35.0	31.0	35.0	2.0	35.0
102-103	28.95940588318631	33.5	29.5	35.0	2.0	35.0
104-105	29.666335914983325	34.5	31.0	35.0	2.0	35.0
106-107	29.775546875299142	35.0	31.0	35.0	2.0	35.0
108-109	29.780520731511864	35.0	31.0	35.0	2.0	35.0
110-111	29.7485817699664	35.0	31.0	35.0	2.0	35.0
112-113	29.69477928342119	35.0	31.0	35.0	2.0	35.0
114-115	29.627559747264872	35.0	31.0	35.0	2.0	35.0
116-117	29.550243715125436	35.0	31.0	35.0	2.0	35.0
118-119	29.460941307621493	35.0	31.0	35.0	2.0	35.0
120-121	29.331043962806298	35.0	31.0	35.0	2.0	35.0
122-123	29.208408841900514	35.0	31.0	35.0	2.0	35.0
124-125	28.282672896613477	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27455.0
3	10078.0
4	5918.0
5	5256.0
6	5618.0
7	6569.0
8	7749.0
9	8596.0
10	10127.0
11	11029.0
12	11821.0
13	13706.0
14	17633.0
15	14860.0
16	7751.0
17	6145.0
18	5820.0
19	5238.0
20	5077.0
21	5461.0
22	6416.0
23	8638.0
24	12179.0
25	16532.0
26	20820.0
27	23259.0
28	27474.0
29	33053.0
30	38490.0
31	46113.0
32	57651.0
33	74389.0
34	104687.0
35	167758.0
36	330968.0
37	510452.0
38	157024.0
39	349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.8358052007799	17.678927421298223	12.687306942405854	24.797960435516025
2	17.06992070577115	19.91058612963644	37.17020908820389	25.849284076388525
3	18.98050552893204	24.402639530920275	29.28646110598337	27.33039383416432
4	13.544637990179446	15.62149050891315	35.33538968075535	35.49848182015206
5	14.550623675540987	37.00841470907999	33.03371081612118	15.407250799257838
6	33.51286140647953	34.84230654546151	16.715478825116055	14.9293532229429
7	29.51340658066133	31.656665049119987	20.439079125386858	18.390849244831827
8	28.208748414299993	32.75340976785735	19.331289998068986	19.706551819773672
9	28.13771139619405	14.029552252522311	18.77791173709742	39.05482461418622
10-11	26.673656017798503	24.53738172305649	26.504292351447113	22.284669907697896
12-13	27.08498808578237	22.378215690007156	26.891012806963417	23.64578341724706
14-15	24.485572170872587	23.334868625823876	24.732604744053795	27.446954459249746
16-17	24.18539589383457	26.24490654465077	25.17904536673015	24.390652194784508
18-19	24.21281174492918	24.932318850013978	27.43987195147635	23.414997453580497
20-21	25.405094460494983	23.862401095898207	27.25538006036412	23.477124383242693
22-23	25.136755809472128	23.481736305750953	27.06549207610896	24.316015808667963
24-25	24.57427759721165	24.118780475662415	27.066491309939916	24.240450617186017
26-27	24.20164738949979	24.156488326204347	27.24628730135893	24.39557698293693
28-29	24.34139099993353	24.225929782839362	27.24375939096875	24.18891982625836
30-31	24.6505981051364	24.113483908048806	27.005348983343	24.230569003471793
32-33	24.272019405886212	24.20363488354663	26.935760673866554	24.588585036700607
34-35	24.49415001746692	24.337262319240615	27.06106255236999	24.107525110922477
36-37	24.714871333073283	24.139580788233665	26.785517920145917	24.36002995854713
38-39	24.756194875585457	24.306896958293585	26.664753557739733	24.27215460838122
40-41	24.743552501508514	24.250296916174722	26.76862748959631	24.237523092720455
42-43	24.74643604548273	24.31300928533272	26.4758487530433	24.46470591614125
44-45	24.66328029834472	24.336989695639915	26.312459360707862	24.687270645307507
46-47	24.762198889892197	24.47814784714531	26.048470859465034	24.71118240349746
48-49	25.11615450354482	24.73004883632118	25.39598126208461	24.75781539804939
50-51	24.939533015985386	24.979826102370154	25.29270615808567	24.78793472355879
52-53	25.159542513483967	24.82100708749561	25.357028351076693	24.66242204794373
54-55	24.958728190909234	24.841594214752373	25.52303384110538	24.676643753233016
56-57	24.870154064329615	24.853795090447615	25.220121209601132	25.055929635621638
58-59	24.92080048341727	24.598011675654146	25.507072494262978	24.974115346665606
60-61	24.914581750120774	24.79944150552777	25.694137832365215	24.591838911986237
62-63	24.771539193219095	24.8571376203309	25.521763311184614	24.849559875265392
64-65	24.82616563196271	24.59200658709089	25.644026277240478	24.937801503705924
66-67	24.875337198176517	24.722732969679928	25.602033118317923	24.799896713825632
68-69	24.943008364974077	24.825865352286684	25.356401187187807	24.87472509555144
70-71	24.780222455523393	24.54415546830165	25.67755054924554	24.998071526929415
72-73	25.142616885117018	24.90473514866788	25.2599437903183	24.6927041758968
74-75	24.820945064287987	25.07012563252582	25.208817638340484	24.900111664845706
76-77	24.836895763461314	24.713994864586077	25.300379589533428	25.14872978241918
78-79	24.876270603099076	24.86732433161503	25.273435811459656	24.98296925382624
80-81	24.814730111903284	24.959006729078933	25.13099518038407	25.09526797863371
82-83	24.961973021017478	24.942470603456222	25.088314769566487	25.007241605959805
84-85	24.73391786151748	24.857282594959386	25.11552792720441	25.29327161631872
86-87	24.55657969994882	24.903667909152556	25.177476166235802	25.36227622466282
88-89	24.679601074648456	24.806778568636318	25.140575037046702	25.373045319668524
90-91	24.61732883091732	24.73924419041992	25.251469234630306	25.39195774403245
92-93	24.689119178070577	24.949131328017664	25.10353576589388	25.258213728017882
94-95	24.531368426350316	24.71842991977335	25.27671506997916	25.473486583897177
96-97	24.6049202674672	24.68883197528379	25.223974242278373	25.48227351497064
98-99	24.563132818782034	24.68117694037076	25.049175933877788	25.706514306969417
100-101	24.472976983559295	24.850425275550382	25.17330371881894	25.503294022071383
102-103	24.693979306970313	24.53564582092863	25.33899202096599	25.43138285113507
104-105	24.432769391632743	24.775061955911404	25.167542842639236	25.624625809816614
106-107	24.500958070796045	24.77656410595831	25.070549346799748	25.6519284764459
108-109	24.55243225617256	24.622736583636435	25.077115914054737	25.74771524613627
110-111	24.58516738533608	24.757172153005204	25.08471692921849	25.572943532440224
112-113	24.573539407516208	24.726166689232528	25.071164521542194	25.62912938170907
114-115	24.183085012547096	24.89575997100835	25.222763916334472	25.698391100110086
116-117	24.34442822456303	24.911686438237126	25.00944336266315	25.734441974536697
118-119	24.283799977406552	24.566737793178035	25.163522138160854	25.985940091254555
120-121	24.020982635908762	24.67193776212568	25.294064875610133	26.013014726355422
122-123	24.099927549490754	24.673596644028958	25.237638490830456	25.988837315649832
124-125	24.014844627111636	24.76057759014998	25.17053922566328	26.05403855707511
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	51.5
2	81.5
3	112.0
4	84.0
5	74.5
6	145.0
7	318.0
8	549.5
9	629.5
10	521.5
11	419.0
12	474.5
13	579.0
14	674.0
15	830.0
16	1026.5
17	1254.5
18	1455.5
19	1646.5
20	1860.0
21	2082.0
22	2353.5
23	2752.5
24	3194.0
25	3806.0
26	4844.0
27	6085.0
28	7108.5
29	8504.0
30	10582.0
31	12365.5
32	14359.5
33	16701.5
34	19358.0
35	21984.0
36	25083.0
37	28606.0
38	31047.0
39	33518.5
40	36311.0
41	38848.0
42	41506.0
43	47021.0
44	52523.5
45	55968.0
46	60702.0
47	64817.5
48	69807.5
49	75986.0
50	76939.0
51	75533.5
52	74896.5
53	75651.0
54	76946.0
55	75702.5
56	72176.5
57	66221.0
58	62351.5
59	59009.0
60	53022.5
61	47621.5
62	42002.5
63	33204.5
64	25160.5
65	20422.0
66	15856.0
67	11847.5
68	9710.5
69	8239.5
70	7187.5
71	5998.0
72	4947.0
73	4828.0
74	4029.5
75	3133.0
76	2185.0
77	1166.0
78	698.5
79	411.0
80	223.0
81	92.5
82	49.5
83	24.5
84	12.5
85	8.5
86	4.5
87	3.5
88	2.5
89	1.0
90	1.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12466092938305694
2	0.0016956949586988876
3	0.022426933324727227
4	0.05311354209343935
5	0.02877211446050371
6	0.022098734300462924
7	0.01575355316468644
8	0.005579383412493115
9	0.0041024878033037605
10-11	0.007657977232833686
12-13	0.004977685201341896
14-15	0.0032819902426430084
16-17	0.010967317394165389
18-19	0.005579383412493115
20-21	0.013784359019100636
22-23	0.009545121622353417
24-25	0.014550156742384003
26-27	0.009627171378419492
28-29	0.014905705685336999
30-31	0.02343888031620882
32-33	0.014221957718119703
34-35	0.022891881942434984
36-37	0.016409951213215042
38-39	0.022891881942434984
40-41	0.011049367150231462
42-43	0.009216922598089117
44-45	0.01862529462699907
46-47	0.017421898204696636
48-49	0.023028631535878443
50-51	0.01651935088796981
52-53	0.023685029584407043
54-55	0.018461195114866925
56-57	0.022755132348991527
58-59	0.018515894952244306
60-61	0.02122353690242479
62-63	0.023985878689982653
64-65	0.01867999446437646
66-67	0.012471562922043431
68-69	0.026173872185077995
70-71	0.01575355316468644
72-73	0.01969194145585805
74-75	0.02012954015487712
76-77	0.014413407148940545
78-79	0.03183530535363718
80-81	0.022973931698501062
82-83	0.009873320646617717
84-85	0.013456159994836334
86-87	0.012991211377128576
88-89	0.021797885194887314
90-91	0.013757009100411943
92-93	0.009162222760711733
94-95	0.008232325525296213
96-97	0.0025708923567370236
98-99	0.0015862952839441208
100-101	0.012389513165977358
102-103	0.003035840974444783
104-105	0.002434142763293565
106-107	3.2819902426430085E-4
108-109	0.021387636414556942
110-111	6.563980485286017E-4
112-113	0.009654521297108185
114-115	0.0025435424380483317
116-117	0.00850582471218313
118-119	0.010693818207278471
120-121	0.004157187640681144
122-123	7.931476419720604E-4
124-125	0.023356830560142743
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1828159.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.12210260568233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.16553530814524	42.304831221755215
2	11.281980904538331	12.212090562215447
3	3.7658465589931467	6.114466015892484
4	1.921270706452911	4.15932841231745
5	1.1100962946153579	3.004037277968008
6	0.7092697537867186	2.30323022337311
7	0.5305293626916662	2.0099355222047928
8	0.38227911398788017	1.6551799545009107
9	0.3109924832482317	1.5148410379161068
>10	1.6919290069444346	16.41676919306276
>50	0.09001356779762576	3.304053533991448
>100	0.037620961545619884	3.650257857020467
>500	0.0021290585503108343	0.7578784184900339
>1k	5.069187024549606E-4	0.5931007692918314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3473	0.1899725352116528	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2981	0.16306021522198016	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	5.469983737738348E-5
14-15	0.0	0.0	0.0	0.0	1.0939967475476695E-4
16-17	0.0	0.0	0.0	0.0	1.6409951213215042E-4
18-19	0.0	0.0	0.0	0.0	1.6409951213215042E-4
20-21	0.0	0.0	0.0	0.0	2.7349918688691736E-4
22-23	0.0	0.0	0.0	0.0	3.008491055756091E-4
24-25	0.0	0.0	0.0	2.734991868869174E-5	3.2819902426430085E-4
26-27	0.0	0.0	0.0	2.7349918688691736E-4	3.2819902426430085E-4
28-29	0.0	0.0	0.0	0.0018050946334536547	3.2819902426430085E-4
30-31	0.0	0.0	0.0	0.005907582436757416	3.2819902426430085E-4
32-33	0.0	0.0	0.0	0.013565559669591103	4.375986990190678E-4
34-35	0.0	0.0	0.0	0.02376707934047312	4.375986990190678E-4
36-37	0.0	0.0	0.0	0.03711383966055469	4.375986990190678E-4
38-39	0.0	0.0	0.0	0.06142791737480165	4.375986990190678E-4
40-41	0.0	0.0	0.0	0.08814878793365347	4.375986990190678E-4
42-43	0.0	0.0	0.0	0.11481495865512792	4.375986990190678E-4
44-45	0.0	0.0	0.0	0.14692376319565204	4.649486177077596E-4
46-47	0.0	0.0	0.0	0.18381880350669716	5.469983737738347E-4
48-49	0.0	0.0	0.0	0.2224368886951299	6.563980485286017E-4
50-51	0.0	0.0	0.0	0.26403611502063007	6.563980485286017E-4
52-53	0.0	0.0	0.0	0.30689343760581	6.563980485286017E-4
54-55	0.0	0.0	0.0	0.3511729559628019	6.563980485286017E-4
56-57	0.0	0.0	0.0	0.4015515061873721	7.931476419720605E-4
58-59	0.0	0.0	0.0	0.4584940368972283	8.204975606607522E-4
60-61	0.0	0.0	0.0	0.5230124950838522	8.204975606607522E-4
62-63	0.0	0.0	0.0	0.586901905140636	8.204975606607522E-4
64-65	0.0	0.0	0.0	0.6549758527567897	8.204975606607522E-4
66-67	0.0	0.0	0.0	0.7324855223205422	8.478474793494439E-4
68-69	0.0	5.469983737738348E-5	0.0	0.821837706676498	9.298972354155191E-4
70-71	0.0	5.469983737738348E-5	0.0	0.9169880737944567	9.845970727929026E-4
72-73	0.0	5.469983737738348E-5	0.0	1.020507516031155	0.0012033964223024365
74-75	0.0	5.469983737738348E-5	0.0	1.124573956641627	0.0012033964223024365
76-77	0.0	5.469983737738348E-5	0.0	1.2418777578974258	0.0012307463409911281
78-79	0.0	5.469983737738348E-5	0.0	1.3768496066261195	0.00125809625967982
80-81	0.0	5.469983737738348E-5	0.0	1.5266177613653955	0.00125809625967982
82-83	0.0	5.469983737738348E-5	0.0	1.6886113297585166	0.00125809625967982
84-85	0.0	5.469983737738348E-5	0.0	1.8482254552257218	0.00125809625967982
86-87	0.0	5.469983737738348E-5	0.0	2.025398228491067	0.00125809625967982
88-89	0.0	5.469983737738348E-5	0.0	2.2233569399598174	0.00125809625967982
90-91	0.0	5.469983737738348E-5	0.0	2.4368230553250565	0.00125809625967982
92-93	0.0	5.469983737738348E-5	0.0	2.6722238054786267	0.0012854461783685118
94-95	0.0	5.469983737738348E-5	0.0	2.9199046691234187	0.0013127960970572034
96-97	0.0	5.469983737738348E-5	0.0	3.180713493738783	0.001340146015745895
98-99	0.0	5.469983737738348E-5	0.0	3.4663013446861024	0.0013674959344345868
100-101	0.0	5.469983737738348E-5	0.0	3.782165555621803	0.0013674959344345868
102-103	0.0	5.469983737738348E-5	0.0	4.122343844271751	0.001476895609189354
104-105	0.0	5.469983737738348E-5	0.0	4.478084236655564	0.001476895609189354
106-107	0.0	5.469983737738348E-5	0.0	4.851437976674895	0.0015315954465667373
108-109	0.0	5.469983737738348E-5	0.0	5.247191300100265	0.0015315954465667373
110-111	0.0	5.469983737738348E-5	0.0	5.6751081279035365	0.0015862952839441208
112-113	5.469983737738348E-5	5.469983737738348E-5	0.0	6.128925328704998	0.0015862952839441208
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2955	0.0	83.52233	1
GTATCAA	6060	0.0	61.28796	1
TCAACGC	8065	0.0	45.484158	4
CAACGCA	8275	0.0	44.375114	5
ATCAACG	8510	0.0	43.245686	3
AACGCAG	8560	0.0	42.815258	6
TATCAAC	8945	0.0	41.209198	2
ACGCAGA	9895	0.0	36.974594	7
CGCAGAG	10435	0.0	34.947178	8
GTACATG	9690	0.0	34.206665	1
TACATGG	9995	0.0	32.64988	2
GCAGAGT	11085	0.0	32.199398	9
GTGGTAT	1080	0.0	32.01577	1
ACATGGG	10110	0.0	31.866175	3
CATGGGG	8195	0.0	29.793308	4
CAGAGTA	10965	0.0	27.343872	10-11
AGAGTAC	10500	0.0	27.223394	10-11
GTATACG	110	7.6655904E-4	27.09799	1
GAGTACT	6130	0.0	26.10645	12-13
ATGGGGG	4175	0.0	25.516369	5
>>END_MODULE