FastQCFastQC Report
Mon 15 Aug 2016
SRR2921539_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921539_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239822
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15250.074.5441361
GTATCAA29000.057.0554051
TCAACGC38450.042.3998154
CAACGCA39200.041.737015
AACGCAG41600.039.3259286
ATCAACG41800.038.7139323
TATCAAC44300.036.797772
ACGCAGA48650.033.2602657
GTACATG66600.032.8869481
CGCAGAG51500.031.4158448
ACATGGG68200.030.7066883
TACATGG70100.030.5533732
GTATAGG3400.029.7591111
CATGGGG57600.028.8198344
GCAGAGT56400.027.8427359
CAGAGTA53000.025.47685610-11
AGAGTAC51550.024.92418510-11
ATGGGGG32050.024.6887055
GTATTAC2201.534354E-724.3483641
GTGGTAT6850.023.4597381
Produced by FastQC (version 0.11.5)