##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921539_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1239822
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.005845193906868	31.0	30.0	34.0	26.0	34.0
2	30.123387066853144	31.0	31.0	34.0	26.0	34.0
3	30.290250535964034	31.0	31.0	34.0	26.0	34.0
4	33.6472412975411	37.0	35.0	37.0	28.0	37.0
5	33.71050602425187	37.0	35.0	37.0	28.0	37.0
6	33.68925861938246	37.0	35.0	37.0	28.0	37.0
7	33.52391311010774	37.0	35.0	37.0	27.0	37.0
8	33.520590859010404	37.0	35.0	37.0	27.0	37.0
9	34.98984934934208	39.0	35.0	39.0	27.0	39.0
10-11	34.88352360258166	39.0	35.0	39.0	27.0	39.0
12-13	34.74972778350441	39.0	35.0	39.0	26.5	39.0
14-15	35.94106129750884	40.0	36.0	41.0	27.0	41.0
16-17	35.87955851727103	40.0	36.0	41.0	27.0	41.0
18-19	35.89646215343815	40.0	36.0	41.0	26.5	41.0
20-21	35.923886654697206	40.0	36.0	41.0	26.5	41.0
22-23	35.90143020530367	40.0	36.0	41.0	26.5	41.0
24-25	35.874432781479925	40.0	36.0	41.0	27.0	41.0
26-27	35.80156748307418	40.0	36.0	41.0	26.5	41.0
28-29	35.7618383929306	40.0	35.5	41.0	27.0	41.0
30-31	35.66371503328703	40.0	35.0	41.0	26.0	41.0
32-33	35.55259585650198	40.0	35.0	41.0	26.0	41.0
34-35	35.38047477783101	40.0	35.0	41.0	25.0	41.0
36-37	35.24264491193091	40.0	35.0	41.0	25.0	41.0
38-39	35.11065983665397	40.0	35.0	41.0	24.5	41.0
40-41	34.94505380611088	39.0	35.0	41.0	24.0	41.0
42-43	34.746668473377625	39.0	34.0	41.0	23.5	41.0
44-45	34.53919554581222	39.0	34.0	41.0	22.5	41.0
46-47	34.2682356822189	39.0	33.0	41.0	21.0	41.0
48-49	34.096404967809896	38.5	33.0	41.0	20.0	41.0
50-51	33.88210807680457	38.0	33.0	40.5	18.5	41.0
52-53	33.679219678308655	38.0	33.0	40.0	18.0	41.0
54-55	33.46492641685661	38.0	33.0	40.0	17.5	41.0
56-57	33.20475519873014	37.0	33.0	40.0	14.5	41.0
58-59	32.95144544942742	37.0	32.5	40.0	14.0	41.0
60-61	32.61953611082882	36.0	32.0	40.0	12.0	41.0
62-63	32.30244381854814	35.5	32.0	39.0	8.5	41.0
64-65	31.986153254257466	35.0	31.5	39.0	8.0	41.0
66-67	31.937137347135312	35.0	32.0	39.0	7.5	41.0
68-69	31.6864682994817	35.0	32.0	37.5	7.0	40.0
70-71	31.39094240947491	35.0	31.5	37.0	4.0	39.5
72-73	31.073720663127446	35.0	31.0	37.0	2.0	39.0
74-75	30.781777545486367	35.0	31.0	36.0	2.0	39.0
76-77	30.5180788048607	35.0	31.0	36.0	2.0	38.0
78-79	30.255568138006907	35.0	31.0	35.0	2.0	37.0
80-81	30.027290611071592	35.0	31.0	35.0	2.0	37.0
82-83	29.848074159032507	35.0	31.0	35.0	2.0	36.0
84-85	29.666888069416416	35.0	30.0	35.0	2.0	36.0
86-87	29.52853877411435	35.0	30.0	35.0	2.0	36.0
88-89	29.413736810606686	35.0	30.0	35.0	2.0	35.0
90-91	29.308231746170016	34.5	30.0	35.0	2.0	35.0
92-93	29.222078653226028	34.0	30.0	35.0	2.0	35.0
94-95	29.158808683827196	34.0	30.0	35.0	2.0	35.0
96-97	29.064214863101316	34.0	30.0	35.0	2.0	35.0
98-99	29.004085263852392	34.0	30.0	35.0	2.0	35.0
100-101	28.942248564713324	34.0	30.0	35.0	2.0	35.0
102-103	27.95568234794995	33.5	28.0	34.5	2.0	35.0
104-105	28.790260618056465	34.0	29.5	35.0	2.0	35.0
106-107	29.122484921222565	35.0	30.0	35.0	2.0	35.0
108-109	29.162607212970897	35.0	31.0	35.0	2.0	35.0
110-111	29.108911198543016	35.0	30.0	35.0	2.0	35.0
112-113	29.036136638969143	35.0	30.0	35.0	2.0	35.0
114-115	28.91624321878463	35.0	30.0	35.0	2.0	35.0
116-117	28.85597045382321	35.0	30.0	35.0	2.0	35.0
118-119	28.698534144417508	35.0	30.0	35.0	2.0	35.0
120-121	28.532734134416067	35.0	30.0	35.0	2.0	35.0
122-123	28.406912443883073	35.0	30.0	35.0	2.0	35.0
124-125	27.536259237213084	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41116.0
3	7383.0
4	4880.0
5	4440.0
6	4646.0
7	5412.0
8	6023.0
9	6371.0
10	6815.0
11	7221.0
12	7280.0
13	7765.0
14	8824.0
15	7695.0
16	5219.0
17	4609.0
18	4246.0
19	3967.0
20	3856.0
21	3931.0
22	4813.0
23	6374.0
24	9533.0
25	13069.0
26	15736.0
27	16358.0
28	19433.0
29	23134.0
30	25996.0
31	30892.0
32	39659.0
33	51322.0
34	72795.0
35	117962.0
36	229979.0
37	326601.0
38	84378.0
39	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.79881838236837	16.920596650738553	12.470245731926752	24.810339234966328
2	17.24194127447065	19.062360074062422	37.83855521805251	25.857143433414414
3	19.704429920702086	23.354279900895765	28.496582904599666	28.444707273802482
4	14.07501597526577	15.005373498486385	34.55796923712458	36.36164128912326
5	15.270558771274443	36.251147734626	32.731939215651465	15.746354278448086
6	34.347723291268146	33.54087537229668	16.79436311948918	15.317038216945985
7	30.240174672489083	30.75375116593564	20.097180130197493	18.90889403137779
8	28.055264648407558	32.84469000772943	19.180696100911877	19.919349242951125
9	27.669592339411388	14.718347187183085	18.714839284316817	38.89722118908871
10-11	26.93720595674846	24.450278804035175	26.260871178900132	22.351644060316232
12-13	27.182178287321502	22.182256953815507	26.359730177959744	24.275834580903243
14-15	24.61462123718161	23.368282194234446	23.803482245871084	28.21361432271286
16-17	24.77674717323354	26.232570223381924	24.355011529753863	24.63567107363067
18-19	24.969234506093787	25.22068441825578	25.883847248213183	23.92623382743725
20-21	26.132435253817626	24.22841467740198	25.492892557714804	24.146257511065595
22-23	25.860185745971787	23.74509296780777	25.394791898257335	24.99992938796311
24-25	25.366341052594198	24.22094139552001	25.41060907062585	25.00210848125994
26-27	24.959138509458363	24.24426920369014	25.55449980734276	25.24209247950873
28-29	24.89718600527334	24.317320320112326	25.64328092943125	25.142212745183084
30-31	25.263801329446533	24.288443498522277	25.401789406792485	25.045965765238705
32-33	24.967276377821857	24.30796196796878	25.30976479341751	25.414996860791856
34-35	25.25276894042391	24.366554701449846	25.461180774472254	24.91949558365399
36-37	25.379357650096836	24.112895416397677	25.313791155584248	25.19395577792124
38-39	25.382002128163546	24.26231532410914	25.217204632568972	25.13847791515834
40-41	25.42156104071533	24.225487245241727	25.37500110953042	24.977950604512518
42-43	25.297838214080357	24.29552813953254	25.282303214937805	25.124330431449295
44-45	25.188652712358127	24.315489556865362	25.167347192412652	25.328510538363858
46-47	25.238982048855686	24.428043089202884	25.12014172062233	25.21283314131911
48-49	25.550221242419312	24.54675261639788	24.70301629653061	25.200009844652193
50-51	25.38567342503824	24.783685978816656	24.685358672754862	25.14528192339024
52-53	25.510955944601683	24.58459735662768	24.86346934345036	25.040977355320276
54-55	25.387105702511413	24.557861499716363	24.895441718895377	25.159591078876847
56-57	25.25364838935088	24.541278481482497	24.807837238988668	25.397235890177956
58-59	25.427340082337203	24.40805006721569	24.815378755969125	25.349231094477986
60-61	25.29605330835412	24.578142945489883	25.010478602874464	25.11532514328153
62-63	25.169125822834243	24.73880711425837	24.84104109208804	25.25102597081934
64-65	25.263720165943898	24.43981023686503	24.99692375222347	25.299545844967604
66-67	25.176553643826775	24.58619924561793	25.004256005809143	25.232991104746148
68-69	25.35448247374107	24.58012735955233	24.75971836644795	25.305671800258654
70-71	25.06553424989157	24.49300477107899	24.981853760881187	25.459607218148257
72-73	25.3463309552768	24.701411706331196	24.809442840920322	25.142814497471676
74-75	25.148140019752002	24.811234435615575	24.77581315638293	25.264812388249496
76-77	25.227426110465895	24.56318978658998	24.76747089579122	25.441913207152904
78-79	25.240014086796776	24.53906282461252	24.923827623111222	25.29709546547948
80-81	25.154614057818314	24.764065321111577	24.736837434592566	25.34448318647754
82-83	25.285157376540035	24.63810555229515	24.720348331851028	25.356388739313786
84-85	25.089019502813777	24.75871560225102	24.710192511229284	25.44207238370592
86-87	24.89371931573793	24.8209563728883	24.790221693037186	25.495102618336585
88-89	25.02364717010433	24.63234448834381	24.788728417545496	25.555279924006364
90-91	24.928037054111055	24.673298107083124	24.817163500377713	25.581501338428115
92-93	25.04303786848402	24.743265198780904	24.753631395782207	25.460065536952868
94-95	24.94879698113938	24.628147906746374	24.834411977997423	25.58864313411682
96-97	24.99803377550193	24.633707500813713	24.815890804562933	25.552367919121423
98-99	24.953759011245456	24.624244071756014	24.757784336706994	25.66421258029154
100-101	24.86057178072866	24.772123390545822	24.80838198734216	25.558922841383357
102-103	25.010143149482193	24.528232639888753	25.003125622603857	25.458498588025197
104-105	24.807351126895586	24.782547849152515	24.838929934168473	25.57117108978343
106-107	24.854293516641253	24.742334151115298	24.83186937858922	25.571502953654228
108-109	24.833355717934662	24.649600323951564	24.883650488851462	25.633393469262312
110-111	24.842631085643855	24.778667195800487	24.8663856449867	25.512316073568957
112-113	24.82542724649745	24.72258318471358	24.920689079412554	25.531300489376413
114-115	24.595239017087398	24.954345413246607	25.00213753807238	25.448278031593617
116-117	24.590039636895707	25.050009921323102	24.861625774145516	25.498324667635675
118-119	24.771098404238113	24.700430797492835	24.822684140054463	25.705786658214585
120-121	24.4150464392776	24.745865070685007	25.135375371680173	25.703713118357218
122-123	24.436171817829017	24.811263896520092	25.073481417652804	25.679082867998087
124-125	24.52672520459506	24.9086521166091	25.01576910481494	25.548853573980896
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	58.0
1	66.5
2	73.5
3	72.0
4	71.5
5	84.0
6	97.0
7	127.0
8	172.0
9	217.5
10	253.5
11	272.5
12	305.0
13	360.5
14	437.5
15	505.0
16	561.5
17	618.0
18	710.5
19	793.0
20	830.0
21	945.5
22	1120.5
23	1346.0
24	1583.5
25	1884.5
26	2385.5
27	3021.0
28	3740.0
29	4461.0
30	5399.5
31	6461.5
32	7599.0
33	8771.5
34	10003.5
35	11308.0
36	13218.0
37	15442.5
38	17444.0
39	19843.0
40	22146.5
41	24385.0
42	26950.0
43	30721.0
44	34419.5
45	37136.5
46	40577.0
47	43965.5
48	48009.5
49	52319.0
50	53015.5
51	53065.0
52	53742.5
53	54526.0
54	55420.5
55	55331.0
56	53523.0
57	49369.0
58	46171.5
59	43113.5
60	39095.0
61	35068.5
62	30640.5
63	24290.5
64	18309.0
65	14626.0
66	11270.0
67	8624.5
68	7032.0
69	5778.5
70	4985.0
71	4168.5
72	3363.5
73	3186.0
74	2715.0
75	2130.0
76	1547.0
77	893.0
78	542.0
79	391.5
80	253.0
81	112.5
82	63.5
83	34.5
84	21.0
85	20.0
86	17.5
87	13.5
88	9.0
89	6.5
90	7.0
91	6.0
92	3.5
93	4.5
94	5.5
95	3.5
96	2.5
97	2.5
98	3.5
99	5.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0406509966753292
2	0.026374753795302873
3	0.025729499879821458
4	0.032746636210681856
5	0.03419845752051504
6	0.04476449038652323
7	0.03823129449227389
8	0.03258532273181151
9	0.02702000771078429
10-11	0.03395648730220951
12-13	0.0332709050170105
14-15	0.031617441858589376
16-17	0.03387583056277433
18-19	0.049119954316022785
20-21	0.05916171837570232
22-23	0.052951149439193684
24-25	0.06279127165028528
26-27	0.04633729680550918
28-29	0.04593401310833329
30-31	0.04714386419986095
32-33	0.0529108210694761
34-35	0.03621487600639447
36-37	0.05016849192868009
38-39	0.05851646446022091
40-41	0.04508711734426393
42-43	0.055209538143378645
44-45	0.05698398641095254
46-47	0.061662077298192806
48-49	0.04601466984776847
50-51	0.04786977485477754
52-53	0.058314822611632955
54-55	0.04524843082313429
56-57	0.04097362363306991
58-59	0.04258675842177345
60-61	0.03157711348887179
62-63	0.04097362363306991
64-65	0.03932016047464878
66-67	0.03202072555576527
68-69	0.027342634668524997
70-71	0.04714386419986095
72-73	0.029399381524122012
74-75	0.03661815970357035
76-77	0.028512157390335063
78-79	0.029318724784686834
80-81	0.022906513999590265
82-83	0.016091019517317808
84-85	0.01661528832364646
86-87	0.014518213098331859
88-89	0.019720572791900773
90-91	0.009799793841374005
92-93	0.01742185571799823
94-95	0.01201785417584137
96-97	0.011009644932901658
98-99	0.009759465471656415
100-101	0.009356181774480532
102-103	0.0055653150210272125
104-105	0.005363673172439269
106-107	0.006976807961142808
108-109	0.010525704496290597
110-111	0.004839404366110619
112-113	0.0065331958942493355
114-115	0.0062912256759438045
116-117	0.005484658281592035
118-119	0.2456804283195491
120-121	0.3672301346483608
122-123	0.031698098598024554
124-125	0.004516777408369911
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1239822.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.94971258653062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.50533114376273	47.66321751165519
2	10.987099400094744	13.17346902390646
3	3.670953064207347	6.60217743353623
4	1.7699520735889298	4.24432472414361
5	1.0330816138558785	3.0966472914544565
6	0.6605496159942202	2.375985577679801
7	0.4593765668839407	1.9277645207575584
8	0.3273782217604795	1.5700984241304183
9	0.262674552243983	1.4172537519740163
>10	1.2470737918762334	12.978316261650471
>50	0.054985244178367136	2.2511477727711897
>100	0.02059620746084012	2.27564679699427
>500	8.130035076556175E-4	0.3324022075197254
>1k	1.3550058460926956E-4	0.09154870182666924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	8.065673943517698E-5
12-13	0.0	0.0	0.0	0.0	8.065673943517698E-5
14-15	0.0	0.0	0.0	0.0	1.2098510915276547E-4
16-17	0.0	0.0	0.0	0.0	1.6131347887035397E-4
18-19	0.0	0.0	0.0	0.0	2.4197021830553095E-4
20-21	0.0	0.0	0.0	0.0	2.4197021830553095E-4
22-23	0.0	0.0	0.0	4.032836971758849E-5	2.8229858802311945E-4
24-25	0.0	0.0	0.0	1.6131347887035397E-4	3.2262695774070793E-4
26-27	0.0	0.0	0.0	5.242688063286504E-4	3.6295532745829646E-4
28-29	0.0	0.0	0.0	0.0016534631584211282	4.0328369717588494E-4
30-31	0.0	0.0	0.0	0.0045571057780874995	4.436120668934734E-4
32-33	0.0	0.0	0.0	0.010082092429397123	4.839404366110619E-4
34-35	0.0	0.0	0.0	0.018430064960937943	4.839404366110619E-4
36-37	0.0	0.0	0.0	0.029842993591015485	4.839404366110619E-4
38-39	0.0	0.0	0.0	0.04831338692167102	4.839404366110619E-4
40-41	0.0	0.0	0.0	0.06871954199877078	4.839404366110619E-4
42-43	0.0	0.0	0.0	0.08908536870615298	4.839404366110619E-4
44-45	0.0	0.0	0.0	0.11090301672336836	4.839404366110619E-4
46-47	0.0	0.0	0.0	0.1394151741137034	4.839404366110619E-4
48-49	0.0	0.0	0.0	0.1692178393350013	4.839404366110619E-4
50-51	0.0	0.0	0.0	0.1997060868414982	4.839404366110619E-4
52-53	0.0	0.0	0.0	0.23148484217895793	5.242688063286504E-4
54-55	0.0	0.0	0.0	0.2657236280691906	5.645971760462389E-4
56-57	0.0	0.0	0.0	0.3071811921388715	5.645971760462389E-4
58-59	0.0	0.0	0.0	0.35234896622257067	6.049255457638274E-4
60-61	0.0	0.0	0.0	0.4000170992287603	6.452539154814159E-4
62-63	0.0	0.0	0.0	0.45079051670320414	6.452539154814159E-4
64-65	0.0	0.0	0.0	0.5022495164628471	6.452539154814159E-4
66-67	0.0	0.0	0.0	0.563508310063864	6.452539154814159E-4
68-69	0.0	0.0	0.0	0.6362606890343936	6.855822851990043E-4
70-71	0.0	0.0	0.0	0.7163125029238068	7.259106549165928E-4
72-73	0.0	0.0	0.0	0.8021312736828352	9.678808732221238E-4
74-75	0.0	0.0	0.0	0.8895631792305669	9.678808732221238E-4
76-77	0.0	0.0	0.0	0.9830443402359371	9.678808732221238E-4
78-79	0.0	0.0	0.0	1.0945926108747868	9.678808732221238E-4
80-81	0.0	0.0	0.0	1.2123514504501451	9.678808732221238E-4
82-83	0.0	0.0	0.0	1.3444671896449654	9.678808732221238E-4
84-85	0.0	0.0	0.0	1.4790026310228404	9.678808732221238E-4
86-87	0.0	0.0	0.0	1.6253139563582515	0.0010485376126573008
88-89	0.0	0.0	0.0	1.7848529869610315	0.0011291943520924778
90-91	0.0	0.0	0.0	1.9678631287394481	0.0011291943520924778
92-93	0.0	0.0	0.0	2.1595035416374286	0.0011291943520924778
94-95	0.0	0.0	0.0	2.368485153513972	0.0011291943520924778
96-97	0.0	0.0	0.0	2.579039571809502	0.0011291943520924778
98-99	0.0	0.0	0.0	2.814315280741913	0.0011291943520924778
100-101	0.0	4.032836971758849E-5	0.0	3.081531058490654	0.0011291943520924778
102-103	0.0	8.065673943517698E-5	0.0	3.37225827578475	0.0014114929401155972
104-105	0.0	8.065673943517698E-5	0.0	3.669559017342812	0.0014518213098331856
106-107	0.0	8.065673943517698E-5	0.0	3.9798051655802205	0.0014518213098331856
108-109	0.0	8.065673943517698E-5	0.0	4.3031983623455625	0.001492149679550774
110-111	0.0	8.065673943517698E-5	0.0	4.656232910853332	0.0016131347887035398
112-113	0.0	8.065673943517698E-5	0.0	5.0334241528219374	0.0016937915281387166
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1525	0.0	74.544136	1
GTATCAA	2900	0.0	57.055405	1
TCAACGC	3845	0.0	42.399815	4
CAACGCA	3920	0.0	41.73701	5
AACGCAG	4160	0.0	39.325928	6
ATCAACG	4180	0.0	38.713932	3
TATCAAC	4430	0.0	36.79777	2
ACGCAGA	4865	0.0	33.260265	7
GTACATG	6660	0.0	32.886948	1
CGCAGAG	5150	0.0	31.415844	8
ACATGGG	6820	0.0	30.706688	3
TACATGG	7010	0.0	30.553373	2
GTATAGG	340	0.0	29.759111	1
CATGGGG	5760	0.0	28.819834	4
GCAGAGT	5640	0.0	27.842735	9
CAGAGTA	5300	0.0	25.476856	10-11
AGAGTAC	5155	0.0	24.924185	10-11
ATGGGGG	3205	0.0	24.688705	5
GTATTAC	220	1.534354E-7	24.348364	1
GTGGTAT	685	0.0	23.459738	1
>>END_MODULE