FastQCFastQC Report
Mon 15 Aug 2016
SRR2921539_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921539_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239822
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22010.17752548349682454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21240.17131491456031592No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18500.081.860651
GTATCAA39650.061.502571
TCAACGC53450.045.111674
CAACGCA54850.043.8304565
ATCAACG55900.043.1345023
TATCAAC58300.041.7672962
AACGCAG57500.041.695186
ACGCAGA67500.035.5181167
CGCAGAG70800.033.610538
GTACATG61700.033.1453131
TATACCG1501.4768375E-731.7373055
TACATGG66550.030.9534912
GCAGAGT75600.030.768319
ACATGGG66000.030.7604053
CATGGGG55300.027.9917354
AGAGTAC69900.027.87350310-11
CAGAGTA72600.026.38618910-11
AGTACTT44200.025.43676812-13
GAGTACT41700.025.24989712-13
GTGGTAT8500.024.550611
Produced by FastQC (version 0.11.5)