##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921539_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1239822
Sequences flagged as poor quality	0
Sequence length	125
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.399603330155458	33.0	31.0	34.0	28.0	34.0
2	31.46146946900442	33.0	31.0	34.0	28.0	34.0
3	31.53657702476646	33.0	31.0	34.0	28.0	34.0
4	35.043539314514504	37.0	35.0	37.0	32.0	37.0
5	34.71542931162699	37.0	35.0	37.0	32.0	37.0
6	34.70176041399491	37.0	35.0	37.0	31.0	37.0
7	34.55870197496092	37.0	35.0	37.0	31.0	37.0
8	34.52576982824954	37.0	35.0	37.0	31.0	37.0
9	36.11318237617981	39.0	37.0	39.0	31.0	39.0
10-11	35.99499807230393	39.0	37.0	39.0	30.0	39.0
12-13	35.908059786001544	39.0	37.0	39.0	30.0	39.0
14-15	37.109139053831925	40.0	37.0	41.0	31.0	41.0
16-17	37.09424941644849	40.0	37.0	41.0	31.0	41.0
18-19	37.122590984834915	40.0	37.0	41.0	30.5	41.0
20-21	37.104200844960005	40.0	37.0	41.0	30.0	41.0
22-23	37.05095247543599	40.0	37.0	41.0	30.0	41.0
24-25	36.981468307547374	40.0	37.0	41.0	30.0	41.0
26-27	36.86204793913966	40.0	37.0	41.0	30.0	41.0
28-29	36.67393182247129	40.0	36.0	41.0	30.0	41.0
30-31	36.61604488386236	40.0	36.0	41.0	30.0	41.0
32-33	36.48224099911117	40.0	36.0	41.0	30.0	41.0
34-35	36.34168775840403	40.0	35.0	41.0	30.0	41.0
36-37	36.19866198534951	40.0	35.0	41.0	29.0	41.0
38-39	36.02514554508631	40.0	35.0	41.0	28.5	41.0
40-41	35.80293219510543	40.0	35.0	41.0	27.5	41.0
42-43	35.61488544323298	39.0	35.0	41.0	27.0	41.0
44-45	35.37908344907575	39.0	35.0	41.0	26.0	41.0
46-47	35.07255235025673	39.0	35.0	41.0	24.5	41.0
48-49	34.76709116308632	39.0	34.0	41.0	24.0	41.0
50-51	34.514772685111254	39.0	34.0	41.0	23.0	41.0
52-53	34.30411784917513	38.0	34.0	41.0	22.5	41.0
54-55	34.07471112788771	38.0	34.0	40.0	22.0	41.0
56-57	33.798148443889524	37.5	33.0	40.0	21.5	41.0
58-59	33.637292692015464	37.0	33.0	40.0	21.5	41.0
60-61	33.503065762665926	37.0	33.0	40.0	22.0	41.0
62-63	33.232477726641406	36.0	33.0	39.5	21.0	41.0
64-65	32.92428066286935	35.5	33.0	39.0	20.0	41.0
66-67	32.618079853398314	35.0	33.0	39.0	20.0	41.0
68-69	32.29640464518293	35.0	33.0	38.5	19.0	40.5
70-71	31.99823966666183	35.0	32.0	37.0	18.0	40.0
72-73	31.696005152352512	35.0	32.0	37.0	18.0	39.0
74-75	31.41863711081107	35.0	32.0	36.0	17.0	39.0
76-77	31.147265897846626	35.0	32.0	36.0	15.0	38.0
78-79	30.885133914384483	35.0	32.0	35.0	13.0	37.0
80-81	30.68174302440189	35.0	31.5	35.0	11.5	37.0
82-83	30.51369874062567	35.0	31.0	35.0	10.5	36.0
84-85	30.33974110799776	35.0	31.0	35.0	9.0	36.0
86-87	30.20991319721702	35.0	31.0	35.0	7.0	36.0
88-89	30.11757736191163	35.0	31.0	35.0	7.0	35.5
90-91	30.03315516259592	35.0	31.0	35.0	7.0	35.0
92-93	30.105615160885996	35.0	31.0	35.0	7.0	35.0
94-95	30.077428453439282	35.0	31.0	35.0	5.5	35.0
96-97	30.067508077772455	35.0	31.5	35.0	2.0	35.0
98-99	30.031298444454123	35.0	31.5	35.0	2.0	35.0
100-101	29.98145701560385	35.0	31.0	35.0	2.0	35.0
102-103	29.099814328185822	33.5	29.5	35.0	2.0	35.0
104-105	29.808027281335548	34.5	31.0	35.0	2.0	35.0
106-107	29.917944672703015	35.0	31.0	35.0	2.0	35.0
108-109	29.92614907623836	35.0	32.0	35.0	2.0	35.0
110-111	29.893327025976312	35.0	32.0	35.0	2.0	35.0
112-113	29.8489464616695	35.0	32.0	35.0	2.0	35.0
114-115	29.79110670725314	35.0	32.0	35.0	2.0	35.0
116-117	29.71693194668267	35.0	31.0	35.0	2.0	35.0
118-119	29.62538251458677	35.0	31.0	35.0	2.0	35.0
120-121	29.509100499910474	35.0	31.0	35.0	2.0	35.0
122-123	29.39264023384002	35.0	31.0	35.0	2.0	35.0
124-125	28.47280859671792	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17576.0
3	6516.0
4	3842.0
5	3298.0
6	3723.0
7	4309.0
8	5027.0
9	5492.0
10	6399.0
11	7190.0
12	7550.0
13	8888.0
14	11804.0
15	10161.0
16	5289.0
17	4456.0
18	4025.0
19	3476.0
20	3377.0
21	3609.0
22	4185.0
23	5703.0
24	7938.0
25	11130.0
26	14063.0
27	15504.0
28	18695.0
29	22122.0
30	25364.0
31	30634.0
32	38132.0
33	49161.0
34	69147.0
35	112584.0
36	230447.0
37	356590.0
38	102193.0
39	223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.944488949473154	17.319544670486923	12.828027185394312	24.907939194645614
2	17.386082616417784	19.418019974157165	36.961627743784895	26.23426966564016
3	19.58570926071144	23.560809219082287	28.76066141945727	28.092820100748998
4	14.072082702136882	15.50786127102318	34.161441557194266	36.25861446964567
5	15.176337609089053	36.18065478039386	32.679397387106896	15.963610223410186
6	34.38584545981119	33.9120536571435	16.224525522477883	15.477575360567423
7	29.79292897921384	31.159070136646385	19.96857108975874	19.079429794381035
8	28.215345252863866	32.91456881100989	18.812623108703473	20.05746282742277
9	27.797122841828898	14.2380209152541	18.58271527265248	39.38214097026453
10-11	27.00273889509318	24.40810867819576	25.977518555399204	22.61163387131186
12-13	27.40224574456329	22.155655054538475	26.38616772345126	24.055931477446972
14-15	24.737191063891224	23.159490522101116	24.207136917144105	27.896181496863555
16-17	24.647020744980125	26.030705071196802	24.68896646157631	24.63330772224676
18-19	24.730540974912333	24.61596165363651	26.86778207747111	23.785715293980047
20-21	25.77628866519933	23.62494045768028	26.75062486664739	23.848146010472995
22-23	25.544914647549334	23.116125714631416	26.669812557346965	24.669147080472285
24-25	24.978865104512867	23.7150467549062	26.62016720767697	24.68592093290397
26-27	24.60728465389263	23.736228545247165	26.81063674979955	24.84585005106066
28-29	24.772203020944296	23.76877099138423	26.789069939752935	24.669956047918536
30-31	25.005395066065862	23.731554420384356	26.601608556594947	24.661441956954835
32-33	24.65011222948459	23.848150381459245	26.514392196168668	24.987345192887496
34-35	24.92066699823767	23.874927544474684	26.653992748195215	24.550412709092427
36-37	25.10477920638448	23.72545690026181	26.419364479980125	24.75039941337358
38-39	25.154852585501704	23.83870645048348	26.230430650727754	24.77601031328707
40-41	25.208448028652153	23.808469404834646	26.294842088493365	24.68824047801984
42-43	25.089395979217706	23.944248206635592	26.14468395405696	24.821671860089747
44-45	25.003125975606906	23.939568236215656	25.924582914183702	25.13272287399374
46-47	25.216909393565263	24.134046395276656	25.623038477998083	25.02600573316
48-49	25.514224183309985	24.332354627019313	25.05736907769401	25.096052111976697
50-51	25.344433841033265	24.626444871628365	24.943189289174384	25.085931998163986
52-53	25.577000492918035	24.496977549237346	24.94128950907461	24.984732448770004
54-55	25.329678972906912	24.503979058013982	25.032973947556297	25.133368021522813
56-57	25.24227161123277	24.501127205856548	24.91026118341892	25.346339999491757
58-59	25.351608207478794	24.331582401297812	25.041232210857228	25.275577180366167
60-61	25.159438762647905	24.371350837882915	25.36946915594565	25.09974124352353
62-63	25.145837686345914	24.551434658827347	25.17766341847893	25.12506423634781
64-65	25.239660427784838	24.22237164095244	25.326420991619596	25.211546939643124
66-67	25.15287020078721	24.42265056217434	25.22143542507011	25.20304381196834
68-69	25.353307466137544	24.402508518305027	24.993576227888408	25.250607787669022
70-71	25.122665523334582	24.239715758824154	25.292390643660738	25.345228074180525
72-73	25.43989570930259	24.557038815371264	24.880285121473495	25.122780353852658
74-75	25.176859418795118	24.72139524021379	24.847079475111013	25.254665865880078
76-77	25.30361751634398	24.439601783207152	24.830671550198783	25.426109150250088
78-79	25.315690258671776	24.4782331753218	24.897244096552832	25.30883246945359
80-81	25.16166870500463	24.6481850187	24.797954455247456	25.39219182104791
82-83	25.299150814600907	24.57100105467576	24.760842691984266	25.369005438739066
84-85	25.095933175494995	24.6590006171022	24.652668261183464	25.592397946219343
86-87	24.958316158326806	24.677277930190556	24.77363274176023	25.590773169722407
88-89	25.020026928454048	24.518970523104176	24.742231447561718	25.718771100880055
90-91	24.88422350087483	24.569664742234444	24.79972541145931	25.746386345431414
92-93	24.989009730320962	24.663859072881014	24.740932120061334	25.606199076736686
94-95	24.949908609427506	24.451132259016852	24.826495038451796	25.772464093103842
96-97	24.954831278694236	24.50274077348209	24.787505363785655	25.75492258403802
98-99	24.904137918796714	24.407082743857487	24.732578266529657	25.95620107081615
100-101	24.87844738541385	24.553285318622123	24.78080196694414	25.787465329019888
102-103	25.01203841646226	24.31514114186296	24.940695171163295	25.732125270511485
104-105	24.838137779896417	24.52881095089568	24.737394830894612	25.895656438313292
106-107	24.864224187472452	24.507236738896268	24.731341941306777	25.8971971323245
108-109	24.920467494226912	24.33797533453669	24.762673066645156	25.978884104591245
110-111	24.951646050704298	24.48121334133452	24.716369203172086	25.850771404789096
112-113	24.870363506495234	24.47313559188734	24.76896935710705	25.887531544510377
114-115	24.537301418302427	24.59315756191361	24.872720585388517	25.996820434395445
116-117	24.623605434439792	24.63147009452918	24.7506096119859	25.994314859045126
118-119	24.622755428070853	24.352329934428372	24.833449354810348	26.19146528269043
120-121	24.339134117077695	24.382892057026474	24.97828234961989	26.29969147627594
122-123	24.408440923136236	24.352142291967283	24.9654586120371	26.27395817285938
124-125	24.45973806679954	24.51443690251304	24.769496443970603	26.256328586716815
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28.0
1	22.0
2	28.0
3	41.0
4	35.5
5	39.5
6	76.0
7	157.0
8	270.0
9	333.5
10	319.0
11	332.5
12	387.0
13	454.0
14	574.0
15	692.0
16	822.5
17	968.0
18	1075.5
19	1209.5
20	1404.0
21	1589.5
22	1759.5
23	1935.0
24	2148.0
25	2493.0
26	2974.0
27	3483.0
28	4024.5
29	4766.0
30	5746.0
31	6713.0
32	7664.0
33	8674.0
34	9771.5
35	11092.5
36	12943.0
37	15127.5
38	17020.0
39	19088.0
40	21506.5
41	23886.0
42	26375.5
43	29883.0
44	33503.0
45	36401.5
46	40180.0
47	43977.5
48	48041.5
49	52219.0
50	52955.5
51	53110.0
52	53763.5
53	54414.0
54	55663.5
55	55674.0
56	53487.5
57	49336.0
58	46561.5
59	43543.0
60	39096.5
61	34899.5
62	30398.0
63	24111.5
64	18058.0
65	14342.5
66	11075.5
67	8505.0
68	6939.0
69	5686.0
70	4932.0
71	4104.5
72	3370.0
73	3245.0
74	2716.0
75	2089.0
76	1463.5
77	832.0
78	515.0
79	341.5
80	194.5
81	71.5
82	47.0
83	20.5
84	6.0
85	4.5
86	4.0
87	2.0
88	1.0
89	0.5
90	0.0
91	1.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12179167654711724
2	0.0016131347887035398
3	0.02468096226716416
4	0.06234765958339181
5	0.028471829020617474
6	0.020002871379923893
7	0.01605069114760022
8	0.005162031323851327
9	0.0045974341478050886
10-11	0.007138121440013163
12-13	0.004436120668934734
14-15	0.0034682397957126105
16-17	0.010041764059679536
18-19	0.005605643390744801
20-21	0.012703436461040376
22-23	0.00883191296815188
24-25	0.013389018746239378
26-27	0.009678808732221237
28-29	0.014235914510308737
30-31	0.021253050841169137
32-33	0.014074601031438384
34-35	0.02165633453834502
36-37	0.015445765601836392
38-39	0.020648125295405308
40-41	0.010041764059679536
42-43	0.009114211556174999
44-45	0.01685725854195199
46-47	0.015445765601836392
48-49	0.021011080622863606
50-51	0.01443755635889668
52-53	0.0213740359503219
54-55	0.016695945063081634
56-57	0.020365826707382188
58-59	0.015849049299012277
60-61	0.01931728909472489
62-63	0.02089009551371084
64-65	0.016413646475058517
66-67	0.010203077538549888
68-69	0.02302749910874303
70-71	0.013751974073697677
72-73	0.016937915281387165
74-75	0.016736273432799223
76-77	0.011735555587818252
78-79	0.02867347086920542
80-81	0.020164184858794246
82-83	0.008428629270975995
84-85	0.012663108091322787
86-87	0.010727346344878538
88-89	0.019438274203877653
90-91	0.01201785417584137
92-93	0.007178449809730751
94-95	0.007097793070295576
96-97	0.0020970752253146015
98-99	0.0016131347887035398
100-101	0.01068701797516095
102-103	0.0033069263168422564
104-105	0.0026616724013608407
106-107	2.0164184858794247E-4
108-109	0.018914005397549002
110-111	4.839404366110619E-4
112-113	0.008509286010411172
114-115	0.0024197021830553093
116-117	0.007985017204082522
118-119	0.008791584598434292
120-121	0.003790866753453318
122-123	4.0328369717588494E-4
124-125	0.024801947376316927
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1239822.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.76086502651417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.1398563109309	46.50326414904336
2	11.159965880720996	13.115384976350997
3	3.691022561099919	6.506630355678329
4	1.7946105724083377	4.218114784817666
5	1.0491076570954543	3.082323671843425
6	0.6892437357069907	2.4300334874549328
7	0.48759622225072713	2.0056103062179225
8	0.33371752151881395	1.568762419115989
9	0.25268669305609415	1.3363279798198828
>10	1.316785154851703	13.45398094753952
>50	0.059955505328446956	2.4027687904269888
>100	0.024351554424863593	2.6816334040366425
>500	5.503153033703265E-4	0.1824615409135593
>1k	5.503153033703265E-4	0.5127031867407147
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2201	0.17752548349682454	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2124	0.17131491456031592	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	8.065673943517698E-5
7	0.0	0.0	0.0	0.0	8.065673943517698E-5
8	0.0	0.0	0.0	0.0	1.6131347887035397E-4
9	0.0	0.0	0.0	0.0	2.4197021830553095E-4
10-11	0.0	0.0	0.0	0.0	2.4197021830553095E-4
12-13	0.0	0.0	0.0	0.0	2.8229858802311945E-4
14-15	0.0	0.0	0.0	0.0	3.6295532745829646E-4
16-17	0.0	0.0	0.0	0.0	4.436120668934734E-4
18-19	0.0	0.0	0.0	0.0	4.839404366110619E-4
20-21	0.0	0.0	0.0	0.0	4.839404366110619E-4
22-23	0.0	0.0	0.0	4.032836971758849E-5	5.645971760462389E-4
24-25	0.0	0.0	0.0	1.6131347887035397E-4	6.452539154814159E-4
26-27	0.0	0.0	0.0	5.242688063286504E-4	6.452539154814159E-4
28-29	0.0	0.0	0.0	0.0016937915281387168	6.452539154814159E-4
30-31	0.0	0.0	0.0	0.004718419256957854	6.452539154814159E-4
32-33	0.0	0.0	0.0	0.010243405908267477	6.452539154814159E-4
34-35	0.0	0.0	0.0	0.018914005397549005	6.452539154814159E-4
36-37	0.0	0.0	0.0	0.030568904245932074	6.452539154814159E-4
38-39	0.0	0.0	0.0	0.05004750681952732	6.452539154814159E-4
40-41	0.0	0.0	0.0	0.07158285624871957	6.452539154814159E-4
42-43	0.0	0.0	0.0	0.09251328013214799	6.855822851990043E-4
44-45	0.0	0.0	0.0	0.11574242108947898	7.259106549165928E-4
46-47	0.0	0.0	0.0	0.14530311609247132	8.065673943517698E-4
48-49	0.0	0.0	0.0	0.17611399055670895	8.872241337869468E-4
50-51	0.0	0.0	0.0	0.2071668352392521	8.872241337869468E-4
52-53	0.0	0.0	0.0	0.23890526220699423	9.275525035045353E-4
54-55	0.0	0.0	0.0	0.27350700342468515	9.678808732221238E-4
56-57	0.0	0.0	0.0	0.3157711348887179	9.678808732221238E-4
58-59	0.0	0.0	0.0	0.3627940139794261	9.678808732221238E-4
60-61	0.0	0.0	0.0	0.41143002785883775	9.678808732221238E-4
62-63	0.0	0.0	0.0	0.46361493827339734	9.678808732221238E-4
64-65	0.0	0.0	0.0	0.5160014905365449	9.678808732221238E-4
66-67	0.0	0.0	0.0	0.5780668515319134	9.678808732221238E-4
68-69	0.0	0.0	0.0	0.6515451411573596	9.678808732221238E-4
70-71	0.0	0.0	0.0	0.732766477768583	0.0010888659823748894
72-73	0.0	0.0	0.0	0.8206823237529258	0.0012501794612452433
74-75	0.0	0.0	0.0	0.9093644087619029	0.0012905078309628317
76-77	0.0	0.0	0.0	1.0060315109749625	0.0012905078309628317
78-79	0.0	0.0	0.0	1.1204834242334787	0.0012905078309628317
80-81	0.0	0.0	0.0	1.2405409808827397	0.0012905078309628317
82-83	0.0	0.0	0.0	1.376124959873272	0.0015324780492683627
84-85	0.0	0.0	0.0	1.512838133215897	0.0015324780492683627
86-87	0.0	0.0	0.0	1.661730474213234	0.0016131347887035396
88-89	0.0	0.0	0.0	1.824979714830032	0.0016937915281387166
90-91	0.0	0.0	0.0	2.010570872270374	0.0017744482675738937
92-93	0.0	0.0	0.0	2.206768390946442	0.0017744482675738937
94-95	0.0	0.0	0.0	2.4192182426186983	0.0017744482675738937
96-97	0.0	0.0	0.0	2.634692722019774	0.0017744482675738937
98-99	0.0	0.0	0.0	2.874081924663379	0.0017744482675738937
100-101	0.0	0.0	0.0	3.1475889280880645	0.0017744482675738937
102-103	0.0	0.0	0.0	3.444526714318668	0.0019357617464442476
104-105	0.0	0.0	0.0	3.74852196524985	0.001976090116161836
106-107	0.0	0.0	0.0	4.064494741987156	0.0020164184858794247
108-109	0.0	0.0	0.0	4.393090298446067	0.0020567468555970133
110-111	0.0	0.0	0.0	4.756408581231822	0.002177731964749779
112-113	0.0	0.0	0.0	5.143359288672084	0.002177731964749779
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1850	0.0	81.86065	1
GTATCAA	3965	0.0	61.50257	1
TCAACGC	5345	0.0	45.11167	4
CAACGCA	5485	0.0	43.830456	5
ATCAACG	5590	0.0	43.134502	3
TATCAAC	5830	0.0	41.767296	2
AACGCAG	5750	0.0	41.69518	6
ACGCAGA	6750	0.0	35.518116	7
CGCAGAG	7080	0.0	33.61053	8
GTACATG	6170	0.0	33.145313	1
TATACCG	150	1.4768375E-7	31.737305	5
TACATGG	6655	0.0	30.953491	2
GCAGAGT	7560	0.0	30.76831	9
ACATGGG	6600	0.0	30.760405	3
CATGGGG	5530	0.0	27.991735	4
AGAGTAC	6990	0.0	27.873503	10-11
CAGAGTA	7260	0.0	26.386189	10-11
AGTACTT	4420	0.0	25.436768	12-13
GAGTACT	4170	0.0	25.249897	12-13
GTGGTAT	850	0.0	24.55061	1
>>END_MODULE