FastQCFastQC Report
Mon 15 Aug 2016
SRR2921538_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921538_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650983
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19050.11538580348798261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19850.074.64181
GTATCAA39400.058.144391
TCAACGC49450.044.9996454
CAACGCA50650.044.2845845
AACGCAG53750.041.2852256
ATCAACG54700.040.8982123
TATCAAC56050.040.656222
ACGCAGA63350.034.8389667
CGCAGAG66650.033.2012528
GTACATG81150.032.923131
TACATGG84300.031.4782892
ACATGGG87650.029.8678913
GCAGAGT73000.029.497439
CATGGGG73350.027.579264
AGAGTAC66000.026.4079910-11
CAGAGTA69000.026.12192510-11
GTATAGG4350.025.9900821
ATGGGGG38700.022.445675
GTACTTT29350.021.99374214-15
GAGTACT29400.021.85250912-13
Produced by FastQC (version 0.11.5)