##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921538_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1650983
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.568887747481348	31.0	30.0	34.0	25.0	34.0
2	29.592776545851773	31.0	30.0	34.0	24.0	34.0
3	29.75525187115797	31.0	30.0	34.0	25.0	34.0
4	33.11333369271519	37.0	35.0	37.0	26.0	37.0
5	33.157651532450664	37.0	35.0	37.0	26.0	37.0
6	33.137585910939116	37.0	35.0	37.0	26.0	37.0
7	32.987627371087406	37.0	35.0	37.0	25.0	37.0
8	32.9471684444964	37.0	35.0	37.0	25.0	37.0
9	34.380264969415194	39.0	35.0	39.0	25.0	39.0
10-11	34.24685323834346	39.0	35.0	39.0	24.0	39.0
12-13	34.09029832530075	39.0	35.0	39.0	22.5	39.0
14-15	35.2031619950054	40.0	35.5	41.0	22.5	41.0
16-17	35.1211260200741	40.0	35.0	41.0	22.0	41.0
18-19	35.13226998703197	40.0	35.0	41.0	22.0	41.0
20-21	35.16303832322925	40.0	35.0	41.0	22.5	41.0
22-23	35.12697829111505	40.0	35.0	41.0	23.0	41.0
24-25	35.08345361520985	40.0	35.0	41.0	23.0	41.0
26-27	35.0079776714842	40.0	35.0	41.0	23.0	41.0
28-29	34.95838812392375	40.0	35.0	41.0	23.0	41.0
30-31	34.85360297471264	40.0	35.0	41.0	23.0	41.0
32-33	34.7393988914483	39.5	34.0	41.0	22.0	41.0
34-35	34.56940804357161	39.0	34.0	41.0	20.5	41.0
36-37	34.41888166019881	39.0	34.0	41.0	18.5	41.0
38-39	34.281875101076146	39.0	34.0	41.0	17.5	41.0
40-41	34.12692892658495	39.0	33.0	41.0	16.0	41.0
42-43	33.914668109847284	39.0	33.0	41.0	15.0	41.0
44-45	33.697332134855415	38.5	33.0	41.0	13.0	41.0
46-47	33.42400042883543	38.0	33.0	40.0	9.0	41.0
48-49	33.25712893470133	38.0	33.0	40.0	8.0	41.0
50-51	33.05483884449446	38.0	32.5	40.0	6.5	41.0
52-53	32.85094122713559	38.0	32.0	40.0	2.0	41.0
54-55	32.64697789135322	37.5	32.0	40.0	2.0	41.0
56-57	32.40348749805419	37.0	32.0	40.0	2.0	41.0
58-59	32.15642953319326	36.5	31.5	40.0	2.0	41.0
60-61	31.847080496891852	36.0	31.0	39.5	2.0	41.0
62-63	31.536298374968126	35.5	31.0	39.0	2.0	41.0
64-65	31.229224649799544	35.0	31.0	39.0	2.0	40.5
66-67	31.194209449764173	35.0	31.0	39.0	2.0	40.5
68-69	30.959559547251544	35.0	31.0	38.0	2.0	40.0
70-71	30.66247926235461	35.0	31.0	37.0	2.0	40.0
72-73	30.346860325030605	35.0	30.0	37.0	2.0	39.0
74-75	30.05258866990151	35.0	30.0	36.0	2.0	39.0
76-77	29.786412397947164	35.0	30.0	36.0	2.0	38.5
78-79	29.519586815854552	35.0	30.0	35.0	2.0	37.0
80-81	29.282521988415386	35.0	30.0	35.0	2.0	37.0
82-83	29.08891642130779	34.5	30.0	35.0	2.0	36.5
84-85	28.89143498146256	34.0	29.0	35.0	2.0	36.0
86-87	28.74471602675497	34.0	29.0	35.0	2.0	36.0
88-89	28.61757995085352	34.0	29.0	35.0	2.0	35.5
90-91	28.506813213703595	34.0	29.0	35.0	2.0	35.0
92-93	28.408972412193222	34.0	29.0	35.0	2.0	35.0
94-95	28.341398730332173	34.0	29.0	35.0	2.0	35.0
96-97	28.24277536473725	34.0	29.0	35.0	2.0	35.0
98-99	28.179150845284294	34.0	29.0	35.0	2.0	35.0
100-101	28.11453540103078	34.0	29.0	35.0	2.0	35.0
102-103	27.13315552007501	32.5	26.0	34.5	2.0	35.0
104-105	27.956626143334	34.0	28.5	35.0	2.0	35.0
106-107	28.286699499631432	34.0	29.0	35.0	2.0	35.0
108-109	28.326946128458015	34.0	29.0	35.0	2.0	35.0
110-111	28.26959302427705	34.0	29.0	35.0	2.0	35.0
112-113	28.198385143880948	34.0	29.0	35.0	2.0	35.0
114-115	28.076834831127883	34.0	29.0	35.0	2.0	35.0
116-117	28.01448954955926	34.0	29.0	35.0	2.0	35.0
118-119	27.851642627452858	34.0	29.0	35.0	2.0	35.0
120-121	27.68051488113445	34.0	29.0	35.0	2.0	35.0
122-123	27.554235264687765	34.0	29.0	35.0	2.0	35.0
124-125	26.702779495609583	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74059.0
3	12715.0
4	8312.0
5	6985.0
6	7409.0
7	8292.0
8	9241.0
9	9540.0
10	9998.0
11	10518.0
12	10678.0
13	11320.0
14	12803.0
15	10866.0
16	7599.0
17	6971.0
18	6119.0
19	5795.0
20	5455.0
21	5740.0
22	6813.0
23	9289.0
24	12948.0
25	17583.0
26	21323.0
27	22915.0
28	26975.0
29	31880.0
30	35928.0
31	42882.0
32	53985.0
33	70034.0
34	97339.0
35	154008.0
36	290635.0
37	408368.0
38	107544.0
39	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.89187014431776	17.070006652989058	12.25252759051519	24.785595612178
2	16.88777893498954	19.225407280982527	38.00507597913141	25.88173780489652
3	19.472713227609397	23.766074430862908	28.731706578209966	28.02950576331773
4	13.52480620248977	14.994783190360838	35.406202247408864	36.07420835974053
5	14.533802231467153	36.72559400967284	33.346097962062395	15.394505796797613
6	33.70663211819842	34.32114804980118	17.08155531128162	14.89066452071878
7	29.894045561741518	31.111739940811912	20.37686962380903	18.61734487363755
8	28.165846257504263	32.911252814476036	19.359959670285193	19.562941257734508
9	27.68946751699794	14.643979317707304	18.89622137372205	38.7703317915727
10-11	26.606344245577912	24.692000588944925	26.606344245577912	22.09531091989925
12-13	26.804668404412467	22.40755955778368	26.74759181635638	24.040180221447464
14-15	24.26359766595504	23.51670570172369	24.27601859989415	27.943678032427123
16-17	24.36796603945986	26.527945583639223	24.708219774040742	24.39586860286018
18-19	24.389525582477674	25.613989160049254	26.34423751902809	23.65224773844498
20-21	25.54935747433443	24.59485700675102	26.090396630732744	23.765388888181807
22-23	25.36798538512375	24.187117201114237	25.924254870691133	24.520642543070885
24-25	24.726794793274397	24.707036073906096	26.061962980281585	24.50420615253792
26-27	24.450642346382256	24.71206520421767	26.160071506484062	24.67722094291601
28-29	24.431026432466876	24.725690096240637	26.211310054954396	24.63197341633809
30-31	24.803802241506933	24.728901525859683	25.954794751738362	24.512501480895015
32-33	24.486321086895128	24.839823812253872	25.944603159404096	24.729251941446904
34-35	24.7952102310357	24.819932244716426	26.026675537506872	24.358181986741002
36-37	24.878296803688944	24.585075882314083	25.886465295575295	24.65016201842168
38-39	24.819337569573253	24.851641197003946	25.759021606156963	24.569999627265837
40-41	24.857304569829104	24.763467061125578	25.95229770093559	24.42693066810973
42-43	24.756610190857366	24.77451897785977	25.799895516924558	24.668975314358303
44-45	24.660836827741775	24.86107718890169	25.757401287380677	24.72068469597586
46-47	24.844099352153943	24.93098187861417	25.587252542382128	24.63766622684976
48-49	24.933469842742497	25.04118461402813	25.304608586336798	24.720736956892576
50-51	24.791503802684606	25.299669726994516	25.218858891615913	24.689967578704966
52-53	24.96855290642054	25.09143217836919	25.320736111990744	24.619278803219526
54-55	24.84605873083252	25.1171735222532	25.400559378775057	24.636208368139226
56-57	24.74650229675721	25.1757978962728	25.29250667242737	24.785193134542617
58-59	24.839946261938508	25.034222564532033	25.3163958655385	24.809435307990963
60-61	24.78405732296518	25.216518281813677	25.425947536350957	24.57347685887019
62-63	24.695627777555593	25.256774296756557	25.3956293530439	24.65196857264395
64-65	24.779152786551474	24.990357769910485	25.428552817152113	24.801936626385928
66-67	24.651537754015102	25.22064428251678	25.37724325221735	24.750574711250767
68-69	24.779997219008532	25.145070510857227	25.317019395143443	24.757912874990797
70-71	24.659315300640326	24.955678795420713	25.557465460366345	24.827540443572616
72-73	24.878345222374605	25.164021510242996	25.334821394760702	24.6228118726217
74-75	24.607398843510335	25.3959035083095	25.30949701849135	24.68720062968881
76-77	24.76581507502309	25.053068586698785	25.32372065095851	24.857395687319613
78-79	24.648143761046157	25.17305770933091	25.283057279148068	24.895741250474867
80-81	24.65219244397229	25.333918578786545	25.189697377815623	24.82419159942554
82-83	24.85163995701254	25.239169232223148	25.187494623538665	24.72169618722565
84-85	24.672865660466055	25.2405043199907	25.096413759512643	24.990216260030607
86-87	24.432890546080717	25.38217472099254	25.29527311608437	24.889661616842375
88-89	24.613774032250298	25.194464748256788	25.2394478318911	24.952313387601812
90-91	24.581401702318924	25.18013916829769	25.269852865901342	24.968606263482044
92-93	24.667501472117014	25.368665353271737	25.198160277799992	24.76567289681126
94-95	24.675041844590734	25.115508032404644	25.282583502902618	24.926866620102004
96-97	24.67763405979819	25.134199881572748	25.277766419665586	24.910399638963472
98-99	24.551206525261765	25.193404469913343	25.149305040237703	25.10608396458719
100-101	24.534652121870018	25.241094058182416	25.286798949362975	24.937454870584585
102-103	24.593476605634905	25.002831807589732	25.44407891748022	24.95961266929515
104-105	24.472830445601527	25.23347983998953	25.28581518354926	25.00787453085968
106-107	24.472401700090288	25.256450678391513	25.23849027185029	25.032657349667915
108-109	24.42763305152022	25.23042636639178	25.210769212596208	25.131171369491796
110-111	24.486626950835756	25.322930895059955	25.249879610756587	24.9405625433477
112-113	24.429815794096445	25.2876967901773	25.331582741221297	24.950904674504958
114-115	24.248552348513368	25.504107395917348	25.320536797863664	24.926803457705617
116-117	24.254701426267896	25.586837823660325	25.183841122503004	24.974619627568778
118-119	24.41794876309748	25.210248837719046	25.25144552716887	25.120356872014604
120-121	24.20300273099889	25.271055819064102	25.42078203528566	25.105159414651347
122-123	24.202924629241203	25.463728708405885	25.24104320177591	25.092303460577003
124-125	24.21576516672931	25.43111450584203	25.28677113944339	25.066349187985278
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	95.0
1	116.5
2	141.5
3	135.0
4	132.5
5	129.5
6	141.5
7	197.5
8	291.0
9	370.0
10	399.0
11	452.0
12	563.0
13	636.0
14	698.5
15	814.5
16	901.5
17	982.0
18	1089.0
19	1208.0
20	1353.0
21	1488.5
22	1703.5
23	2070.5
24	2443.0
25	2993.0
26	3991.0
27	5115.0
28	5855.5
29	7014.5
30	8944.0
31	10725.0
32	13001.5
33	15487.5
34	18152.5
35	20938.5
36	23818.5
37	26791.5
38	29022.5
39	31752.5
40	34331.0
41	36462.5
42	38981.5
43	43170.0
44	47483.5
45	50824.5
46	54549.0
47	58124.5
48	62500.0
49	66785.5
50	67634.0
51	67858.0
52	68047.5
53	68322.5
54	69844.5
55	69574.5
56	66520.5
57	61282.0
58	57419.0
59	53842.5
60	48645.5
61	43272.0
62	37308.0
63	29477.5
64	22354.5
65	17590.5
66	13671.0
67	10620.0
68	8341.5
69	6798.0
70	5796.0
71	4718.0
72	3848.0
73	3430.5
74	2808.0
75	2212.0
76	1625.5
77	1013.0
78	627.0
79	395.0
80	234.5
81	121.0
82	78.5
83	51.5
84	40.0
85	29.0
86	24.0
87	19.0
88	14.5
89	11.5
90	11.0
91	8.0
92	5.0
93	6.5
94	6.0
95	6.0
96	14.0
97	15.5
98	8.5
99	8.5
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03616027542379297
2	0.028104468671088678
3	0.027741048817583224
4	0.03325291659574932
5	0.03567571561911903
6	0.044882351907923945
7	0.03943105410534209
8	0.03555457566795055
9	0.029618718060694747
10-11	0.03476716598535539
12-13	0.034464316107434174
14-15	0.032919781730035985
16-17	0.03616027542379297
18-19	0.04791085068713609
20-21	0.06117567534008528
22-23	0.05351357342867855
24-25	0.0653247186676059
26-27	0.04742629088246214
28-29	0.04824398555284942
30-31	0.04836512550401791
32-33	0.05602722741542463
34-35	0.038280224569241475
36-37	0.052211318953617324
38-39	0.061387670254630114
40-41	0.04785028071155185
42-43	0.05784432668295192
44-45	0.05851059641437859
46-47	0.06384075426579196
48-49	0.047941135674928206
50-51	0.05045478966167429
52-53	0.0599339908406083
54-55	0.04600289645623244
56-57	0.04370123738403121
58-59	0.045790901541687586
60-61	0.03301063669341235
62-63	0.04245955288455423
64-65	0.041823568140919684
66-67	0.03567571561911903
68-69	0.0298307129752396
70-71	0.04927367513778155
72-73	0.0311026824625087
74-75	0.03900706427625239
76-77	0.03176895219393537
78-79	0.03161752725497476
80-81	0.023834285392399557
82-83	0.016293323432161326
84-85	0.017262443041509208
86-87	0.016081328517616473
88-89	0.02195661614928803
90-91	0.010660315702826741
92-93	0.01811042269968861
94-95	0.014052234335544339
96-97	0.010932880592955833
98-99	0.010175755898152797
100-101	0.010599745727242498
102-103	0.0056935777049188264
104-105	0.0055118677781660986
106-107	0.007843811838159449
108-109	0.011175160495292803
110-111	0.005330157851413371
112-113	0.006844407241019442
114-115	0.007238112082317019
116-117	0.006117567534008527
118-119	0.24264332219047685
120-121	0.36223873898156433
122-123	0.027195919037325038
124-125	0.003543343571678206
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1650983.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.48183353978024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.70763846032868	48.60120149825816
2	9.504492709248161	11.306893064231081
3	3.1342874193165624	5.592994876248455
4	1.5410561650196106	3.6665938513259437
5	0.938368516763026	2.790793995653439
6	0.6230884421762254	2.2237465798852334
7	0.44584938379866296	1.8563957181647963
8	0.32534625916376125	1.548175362429524
9	0.24395648462889027	1.3059881108681048
>10	1.4333829545100008	15.327396062144812
>50	0.07839208451556377	3.1545330864877577
>100	0.023627544186200104	2.2819230741797907
>500	3.081458068096272E-4	0.13954537735955466
>1k	2.0543053787308477E-4	0.20381934276334254
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1905	0.11538580348798261	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.056997558424284E-5
2	0.0	0.0	0.0	0.0	6.056997558424284E-5
3	0.0	0.0	0.0	0.0	6.056997558424284E-5
4	0.0	0.0	0.0	0.0	6.056997558424284E-5
5	0.0	0.0	0.0	0.0	6.056997558424284E-5
6	0.0	0.0	0.0	0.0	1.2113995116848568E-4
7	0.0	0.0	0.0	0.0	1.2113995116848568E-4
8	0.0	0.0	0.0	0.0	1.8170992675272852E-4
9	0.0	0.0	0.0	0.0	2.4227990233697137E-4
10-11	0.0	0.0	0.0	0.0	2.4227990233697137E-4
12-13	0.0	0.0	0.0	0.0	3.9370484129757846E-4
14-15	0.0	0.0	0.0	0.0	4.845598046739427E-4
16-17	0.0	0.0	0.0	0.0	6.056997558424284E-4
18-19	0.0	0.0	0.0	0.0	6.662697314266712E-4
20-21	0.0	0.0	0.0	0.0	7.268397070109141E-4
22-23	0.0	0.0	0.0	6.056997558424284E-5	7.874096825951569E-4
24-25	0.0	0.0	0.0	6.056997558424284E-5	9.691196093478855E-4
26-27	0.0	0.0	0.0	1.514249389606071E-4	9.691196093478855E-4
28-29	0.0	0.0	0.0	9.994045971400069E-4	9.994045971400069E-4
30-31	0.0	0.0	0.0	0.003543343571678206	0.001090259560516371
32-33	0.0	0.0	0.0	0.008994641374260062	0.0012113995116848568
34-35	0.0	0.0	0.0	0.016505318346706176	0.0012113995116848568
36-37	0.0	0.0	0.0	0.0277107638297911	0.0012113995116848568
38-39	0.0	0.0	0.0	0.043428672493902115	0.0012416844994769783
40-41	0.0	0.0	0.0	0.06459787896059499	0.0012719694872690996
42-43	0.0	0.0	0.0	0.08458597090339512	0.001302254475061221
44-45	0.0	0.0	0.0	0.10923795096618197	0.0013931094384375853
46-47	0.0	0.0	0.0	0.13646415499129913	0.0014839644018139497
48-49	0.0	0.0	0.0	0.16417491882109023	0.001665674328566678
50-51	0.0	0.0	0.0	0.19564102113710438	0.0016959593163587995
52-53	0.0	3.028498779212142E-5	0.0	0.22586543895364156	0.0019079542309036494
54-55	0.0	6.056997558424284E-5	0.0	0.2597543402930254	0.002029094182072135
56-57	0.0	6.056997558424284E-5	0.0	0.2974894350820087	0.0020593791698642564
58-59	0.0	6.056997558424284E-5	0.0	0.3388587284060466	0.0020593791698642564
60-61	0.0	6.056997558424284E-5	0.0	0.384104500167476	0.0020593791698642564
62-63	0.0	6.056997558424284E-5	0.0	0.4345895748169424	0.0021199491454484995
64-65	0.0	6.056997558424284E-5	0.0	0.48683117875835186	0.0022108041088248634
66-67	0.0	6.056997558424284E-5	0.0	0.5445543654901353	0.002241089096616985
68-69	0.0	6.056997558424284E-5	0.0	0.6109390587304655	0.002241089096616985
70-71	0.0	6.056997558424284E-5	0.0	0.6865606732473926	0.0023319440599933496
72-73	0.0	6.056997558424284E-5	0.0	0.7651502165679477	0.002543938974538199
74-75	0.0	6.056997558424284E-5	0.0	0.8453448642414852	0.0026045089501224423
76-77	0.0	6.056997558424284E-5	0.0	0.931566224485655	0.0026045089501224423
78-79	0.0	6.056997558424284E-5	0.0	1.0352317376980866	0.002725648901290928
80-81	0.0	6.056997558424284E-5	0.0	1.1475284724312727	0.0027559338890830493
82-83	0.0	6.056997558424284E-5	0.0	1.268032438856124	0.0029073588280436563
84-85	0.0	6.056997558424284E-5	0.0	1.3867193060134477	0.0029073588280436563
86-87	0.0	6.056997558424284E-5	0.0	1.5191555576283946	0.0029376438158357776
88-89	0.0	6.056997558424284E-5	0.0	1.6669462980539471	0.0029679288036278994
90-91	0.0	6.056997558424284E-5	0.0	1.8286378478760836	0.0029679288036278994
92-93	0.0	6.056997558424284E-5	0.0	2.0121345889085474	0.0029679288036278994
94-95	0.0	6.056997558424284E-5	0.0	2.201355192633722	0.0031496387303806277
96-97	0.0	6.056997558424284E-5	0.0	2.400690982281465	0.003301063669341235
98-99	0.0	6.056997558424284E-5	0.0	2.6192577391772054	0.003391918632717599
100-101	0.0	6.056997558424284E-5	0.0	2.863385025769496	0.003391918632717599
102-103	0.0	6.056997558424284E-5	0.0	3.132830562156	0.0034827735960939635
104-105	0.0	6.056997558424284E-5	0.0	3.413390689062213	0.0035130585838860848
106-107	0.0	6.056997558424284E-5	0.0	3.700401518368148	0.0035433435716782065
108-109	0.0	6.056997558424284E-5	0.0	3.995771004304708	0.003664483522846692
110-111	0.0	6.056997558424284E-5	0.0	4.32684649084818	0.003755338486223056
112-113	0.0	6.056997558424284E-5	0.0	4.6811202780404155	0.003755338486223056
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1985	0.0	74.6418	1
GTATCAA	3940	0.0	58.14439	1
TCAACGC	4945	0.0	44.999645	4
CAACGCA	5065	0.0	44.284584	5
AACGCAG	5375	0.0	41.285225	6
ATCAACG	5470	0.0	40.898212	3
TATCAAC	5605	0.0	40.65622	2
ACGCAGA	6335	0.0	34.838966	7
CGCAGAG	6665	0.0	33.201252	8
GTACATG	8115	0.0	32.92313	1
TACATGG	8430	0.0	31.478289	2
ACATGGG	8765	0.0	29.867891	3
GCAGAGT	7300	0.0	29.49743	9
CATGGGG	7335	0.0	27.57926	4
AGAGTAC	6600	0.0	26.40799	10-11
CAGAGTA	6900	0.0	26.121925	10-11
GTATAGG	435	0.0	25.990082	1
ATGGGGG	3870	0.0	22.44567	5
GTACTTT	2935	0.0	21.993742	14-15
GAGTACT	2940	0.0	21.852509	12-13
>>END_MODULE