FastQCFastQC Report
Mon 15 Aug 2016
SRR2921538_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921538_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650983
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30010.18177049672831277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26790.16226696459018658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.078.288451
GTATCAA49950.061.7084351
TCAACGC67850.044.848374
CAACGCA69150.044.0632865
ATCAACG71700.042.357143
AACGCAG73050.041.8598146
TATCAAC74200.041.170782
ACGCAGA85400.035.665917
GTACATG80900.033.973631
CGCAGAG90550.033.5717288
GTGGTAT11050.032.9122161
TACATGG85150.032.029962
GCAGAGT95350.031.194459
ACATGGG86900.031.042313
CATGGGG69800.030.030524
AGAGTAC89050.027.72312710-11
TGGTATC11850.026.6337992
CAGAGTA92150.026.3708910-11
GAGTACT54700.025.82646212-13
GTACTTT56850.024.85199414-15
Produced by FastQC (version 0.11.5)