##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921538_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1650983
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.134624644832805	31.0	31.0	34.0	28.0	34.0
2	31.200965121990958	33.0	31.0	34.0	27.0	34.0
3	31.273687251776668	33.0	31.0	34.0	27.0	34.0
4	34.76977715700283	37.0	35.0	37.0	32.0	37.0
5	34.41767540913504	37.0	35.0	37.0	30.0	37.0
6	34.393816290052655	37.0	35.0	37.0	30.0	37.0
7	34.23152994307028	37.0	35.0	37.0	30.0	37.0
8	34.20213896811778	37.0	35.0	37.0	30.0	37.0
9	35.74319784031695	39.0	37.0	39.0	30.0	39.0
10-11	35.61517380857344	39.0	35.0	39.0	30.0	39.0
12-13	35.519819707410676	39.0	35.0	39.0	30.0	39.0
14-15	36.68275869587997	40.0	37.0	41.0	29.5	41.0
16-17	36.65721240012768	40.0	37.0	41.0	29.5	41.0
18-19	36.68893047354213	40.0	37.0	41.0	29.0	41.0
20-21	36.67415806219689	40.0	37.0	41.0	29.0	41.0
22-23	36.61532856486106	40.0	37.0	41.0	29.0	41.0
24-25	36.54935150755641	40.0	36.0	41.0	29.0	41.0
26-27	36.424104003493674	40.0	36.0	41.0	29.0	41.0
28-29	36.237020914206866	40.0	36.0	41.0	28.0	41.0
30-31	36.18512001637812	40.0	36.0	41.0	28.5	41.0
32-33	36.05348480269028	40.0	35.0	41.0	28.0	41.0
34-35	35.92271997955157	40.0	35.0	41.0	27.5	41.0
36-37	35.7825383423088	40.0	35.0	41.0	27.0	41.0
38-39	35.61061924925938	40.0	35.0	41.0	26.5	41.0
40-41	35.39663006826842	39.0	35.0	41.0	25.5	41.0
42-43	35.220730922123366	39.0	35.0	41.0	25.0	41.0
44-45	34.992186775999514	39.0	35.0	41.0	24.0	41.0
46-47	34.694418416179936	39.0	34.0	41.0	23.0	41.0
48-49	34.41385798642385	39.0	34.0	41.0	22.0	41.0
50-51	34.181384968833726	38.5	34.0	41.0	20.5	41.0
52-53	33.97605214590338	38.0	33.0	40.5	20.0	41.0
54-55	33.749098567338365	38.0	33.0	40.0	19.0	41.0
56-57	33.483094011264804	37.5	33.0	40.0	18.0	41.0
58-59	33.32736648408857	37.0	33.0	40.0	18.0	41.0
60-61	33.21255003837108	37.0	33.0	40.0	18.0	41.0
62-63	32.943724738534556	36.0	33.0	40.0	17.0	41.0
64-65	32.64707843751268	36.0	33.0	39.0	14.5	41.0
66-67	32.34114494213448	35.0	32.0	39.0	13.0	41.0
68-69	32.029106296067255	35.0	32.0	38.5	11.5	40.5
70-71	31.736749257866375	35.0	32.0	37.0	9.0	40.0
72-73	31.42726333342015	35.0	32.0	37.0	7.5	39.0
74-75	31.141184978888337	35.0	32.0	36.5	7.0	39.0
76-77	30.85609330925879	35.0	31.0	36.0	7.0	39.0
78-79	30.585443944607547	35.0	31.0	35.5	3.5	37.0
80-81	30.368809369932944	35.0	31.0	35.0	2.0	37.0
82-83	30.189858405568074	35.0	31.0	35.0	2.0	36.5
84-85	30.002866474094525	35.0	31.0	35.0	2.0	36.0
86-87	29.865383532113896	35.0	31.0	35.0	2.0	36.0
88-89	29.763038141519324	35.0	31.0	35.0	2.0	35.5
90-91	29.663984123398	35.0	31.0	35.0	2.0	35.0
92-93	29.723675228636516	35.0	31.0	35.0	2.0	35.0
94-95	29.692336626119108	35.0	31.0	35.0	2.0	35.0
96-97	29.67342607404195	35.0	31.0	35.0	2.0	35.0
98-99	29.632675503018504	35.0	31.0	35.0	2.0	35.0
100-101	29.583937872164643	35.0	31.0	35.0	2.0	35.0
102-103	28.706844346671044	33.5	29.0	35.0	2.0	35.0
104-105	29.40768287741303	34.5	30.5	35.0	2.0	35.0
106-107	29.515567089424906	35.0	31.0	35.0	2.0	35.0
108-109	29.517188547671296	35.0	31.0	35.0	2.0	35.0
110-111	29.479883196859085	35.0	31.0	35.0	2.0	35.0
112-113	29.4352718955919	35.0	31.0	35.0	2.0	35.0
114-115	29.37150170534766	35.0	31.0	35.0	2.0	35.0
116-117	29.295961557447896	35.0	31.0	35.0	2.0	35.0
118-119	29.19815588652336	35.0	31.0	35.0	2.0	35.0
120-121	29.077562579384526	35.0	31.0	35.0	2.0	35.0
122-123	28.959448704196227	35.0	31.0	35.0	2.0	35.0
124-125	28.048527453038584	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31362.0
3	11151.0
4	6229.0
5	5190.0
6	5663.0
7	6505.0
8	7493.0
9	7985.0
10	9118.0
11	10095.0
12	10528.0
13	12254.0
14	15530.0
15	13191.0
16	7217.0
17	6128.0
18	5388.0
19	4993.0
20	4852.0
21	4992.0
22	6050.0
23	7954.0
24	11271.0
25	15728.0
26	19507.0
27	21263.0
28	25129.0
29	29671.0
30	34315.0
31	40862.0
32	51533.0
33	65558.0
34	91092.0
35	146282.0
36	293766.0
37	462591.0
38	142254.0
39	293.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.96173345623908	17.5926644673006	12.625715602561616	24.8198864738987
2	17.151042881241462	19.66383093421686	37.06266978809234	26.12245639644933
3	19.50050102567893	23.988709655921976	28.808431286781765	27.702358031617326
4	13.630887085532814	15.555966327476803	34.9508790855813	35.862267501409086
5	14.528667579912549	36.86910149086078	33.12210735627434	15.480123572952332
6	33.79079253549686	34.85843967093955	16.427698606418485	14.9230691871451
7	29.68705227951631	31.452945164028122	20.038976860442364	18.8210256960132
8	28.246044851067854	33.11549071225996	18.817410772155075	19.821053664517112
9	27.75068144648374	14.389242231510085	18.699133805802894	39.160942516203285
10-11	26.69266140350983	24.748992486887033	26.292896918975533	22.2654491906276
12-13	27.047078388668456	22.407444929130886	26.772227557710345	23.773249124490313
14-15	24.45087319679705	23.37486480994042	24.476495328503894	27.697766664758632
16-17	24.232272118338027	26.396197229640357	24.908067105788025	24.46346354623359
18-19	24.18246097773648	25.10932775733251	27.17131331719451	23.536897947736502
20-21	25.310842237833914	24.10548614968769	27.052073684982105	23.531597927496296
22-23	25.093506362127748	23.71879852691702	26.938888379042336	24.248806731912893
24-25	24.50603408821403	24.270776887911012	27.012627317398525	24.210561706476433
26-27	24.20502796020301	24.2866839287061	27.16323954911125	24.345048561979638
28-29	24.3457872164876	24.25355604913786	27.177662671396252	24.222994062978287
30-31	24.53786725861016	24.263241181431326	26.960338251117165	24.238553308841347
32-33	24.23849584661634	24.35510862404495	26.921165219103294	24.48523031023542
34-35	24.470145263817937	24.361700999777355	26.980054434173876	24.18809930223083
36-37	24.65654256279404	24.19056238694286	26.797585771975037	24.35530927828806
38-39	24.625691297669132	24.439245018279614	26.65485082934516	24.280212854706097
40-41	24.64836767120931	24.395551008268107	26.731023303232792	24.22505801728979
42-43	24.663050325894694	24.417962976424125	26.546546000823824	24.37244069685736
44-45	24.57477488884157	24.490900593057003	26.359034691365917	24.575289826735514
46-47	24.78759749281895	24.61866900587842	26.053213230641454	24.54052027066118
48-49	24.986868626285645	24.874123590611326	25.52118073919789	24.617827043905134
50-51	24.75650048887699	25.179315475288995	25.392918977271762	24.671265058562252
52-53	25.015327703885724	25.0402276497238	25.403488173434486	24.540956472955994
54-55	24.861474805271122	25.05648386964865	25.473702703562296	24.608338621517937
56-57	24.72976202700971	25.14166573520329	25.40907902656473	24.71949321122227
58-59	24.78930697495916	24.90850002438384	25.5526877199657	24.7495052806913
60-61	24.77537595863925	25.032669007970814	25.70310308624436	24.488851947145573
62-63	24.664550696646476	25.106111514326113	25.62334077699195	24.605997012035463
64-65	24.814788787429205	24.808488365119167	25.663740403214913	24.71298244423672
66-67	24.72059648890064	25.00455084176186	25.598537915251057	24.676314754086444
68-69	24.776782671519516	24.989162883899727	25.450668023767438	24.783386420813322
70-71	24.68360469085597	24.77680511842088	25.757938771226417	24.781651419496733
72-73	24.987459713572587	25.101261298374006	25.333983570407348	24.577295417646063
74-75	24.635525682073588	25.35244886151945	25.323369672223013	24.68865578418395
76-77	24.779942069054922	25.010381591250326	25.336110642471326	24.87356569722343
78-79	24.795114682157763	25.088087130145382	25.26985061767777	24.846947570019083
80-81	24.67773658473934	25.256031108131776	25.194872622838744	24.871359684290145
82-83	24.89053937977565	25.136962071016743	25.19617499645631	24.776323552751293
84-85	24.65503976931977	25.239827474451314	25.100983177547448	25.00414957868146
86-87	24.5216429440498	25.194203590362257	25.303152054437277	24.981001411150665
88-89	24.541171089439416	25.09892360096144	25.263162677563432	25.096742632035706
90-91	24.570982721182762	25.09952122589433	25.284615713131714	25.0448803397912
92-93	24.640465408869797	25.231857901038225	25.227435935256366	24.900240754835618
94-95	24.575002460834867	25.004474933557457	25.355049254556327	25.06547335105134
96-97	24.590433525363835	24.987234645686463	25.25919877791797	25.16313305103174
98-99	24.520839680646606	25.020548638035667	25.182787479534646	25.27582420178308
100-101	24.46016624807819	25.115672170711406	25.29976629314763	25.12439528806278
102-103	24.553472323385527	24.91535849263462	25.350051955454294	25.181117228525558
104-105	24.434274437545316	25.11767588387899	25.197236956315006	25.250812722260683
106-107	24.470950586924133	25.06859562199421	25.137494094416653	25.32295969666501
108-109	24.435579142509223	25.020174115337777	25.162483415423203	25.381763326729796
110-111	24.468821293121124	25.158747152060794	25.127129462023795	25.245302092794287
112-113	24.456165856465706	25.044053730710402	25.215634606938952	25.28414580588494
114-115	24.193261160177524	25.26350187802442	25.25574870512927	25.287488256668787
116-117	24.184475782026123	25.32328996147416	25.114396307334445	25.377837949165272
118-119	24.282830889412665	24.94180203788274	25.215452096550163	25.559914976154435
120-121	24.061186712330258	25.12081186542727	25.28914286268091	25.528858559561552
122-123	24.083458537089133	25.150221793115644	25.169331707224003	25.596987962571223
124-125	24.062799045055137	25.213040492461225	25.132189463435733	25.591970999047902
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26.0
1	26.0
2	53.5
3	86.0
4	77.5
5	72.0
6	126.0
7	258.5
8	441.5
9	521.5
10	482.5
11	465.5
12	503.5
13	607.0
14	746.5
15	907.0
16	1048.0
17	1228.0
18	1445.5
19	1634.0
20	1855.5
21	2073.5
22	2330.0
23	2674.5
24	3058.5
25	3613.0
26	4539.0
27	5597.0
28	6321.0
29	7455.0
30	9435.5
31	11048.5
32	13113.5
33	15674.5
34	18046.5
35	20526.5
36	23536.0
37	26354.5
38	28312.0
39	31201.0
40	33738.5
41	35586.0
42	38081.5
43	42200.0
44	46678.0
45	50131.5
46	54237.5
47	57984.0
48	62302.5
49	66456.5
50	67177.0
51	67899.5
52	68647.5
53	69150.0
54	70293.5
55	69808.0
56	66812.5
57	61612.0
58	57992.5
59	54445.0
60	48895.0
61	43432.5
62	37202.0
63	29083.0
64	21787.5
65	17074.5
66	13288.5
67	10304.0
68	8250.5
69	6706.0
70	5649.5
71	4622.5
72	3787.0
73	3414.0
74	2821.0
75	2198.0
76	1606.0
77	944.0
78	517.0
79	302.5
80	183.0
81	87.0
82	43.5
83	17.5
84	9.0
85	6.5
86	3.5
87	1.5
88	1.5
89	1.5
90	0.5
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12253306060692327
2	0.0016959593163587995
3	0.022350320990585607
4	0.058934586243468284
5	0.027862188768751708
6	0.020836071600979538
7	0.01574819365190314
8	0.005875287631671556
9	0.005027307973492156
10-11	0.008237516679457027
12-13	0.005299872863621249
14-15	0.004027903376352149
16-17	0.01129630044646129
18-19	0.00596614259504792
20-21	0.013688814482038883
22-23	0.009660911105686733
24-25	0.014324799225673432
26-27	0.009358061227765519
28-29	0.014506509152426161
30-31	0.022713740844091064
32-33	0.013355679616325547
34-35	0.02213832607604076
36-37	0.016293323432161326
38-39	0.0226228858807147
40-41	0.011023735556332197
42-43	0.008661506508546727
44-45	0.018080137711896486
46-47	0.017232158053717087
48-49	0.021562911307990453
50-51	0.01580876362748738
52-53	0.02283488079525955
54-55	0.017595577907222545
56-57	0.021472056344614087
58-59	0.01814070768748073
60-61	0.020351511796305594
62-63	0.021865761185911665
64-65	0.018170992675272852
66-67	0.011478010373214019
68-69	0.024561125099410476
70-71	0.014506509152426161
72-73	0.01835270260202558
74-75	0.019261252235789224
76-77	0.013597959518662519
78-79	0.029467293121734142
80-81	0.021411486369029845
82-83	0.009751766069063098
84-85	0.012901404799443725
86-87	0.012265420055809176
88-89	0.02062407668643469
90-91	0.012386560006977661
92-93	0.00808609174049642
94-95	0.007298682057901263
96-97	0.0018170992675272855
98-99	0.0013325394628533427
100-101	0.01193228519009584
102-103	0.0029982137914200207
104-105	0.003028498779212142
106-107	1.8170992675272855E-4
108-109	0.021381201381237724
110-111	5.148447924660642E-4
112-113	0.009570056142310369
114-115	0.0027862188768751706
116-117	0.008661506508546727
118-119	0.009206636288804911
120-121	0.004179328315312756
122-123	4.5427481688182136E-4
124-125	0.025227394830837146
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1650983.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.542847981101616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.63154723561937	46.39768865060259
2	10.029471256538992	11.542486796917029
3	3.3122745460106304	5.7179313201828625
4	1.6832895514367978	3.874450990660176
5	0.9588084921779884	2.758628565419362
6	0.6680552947763565	2.3065082562171546
7	0.4569292854300508	1.8405108686730363
8	0.35278734480302976	1.6240310841325778
9	0.2689674483675431	1.3929437693950502
>10	1.523634640744127	15.925362232802561
>50	0.085016245029859	3.3262322180577204
>100	0.028477688642034808	2.7026952012994347
>500	4.2341167040988814E-4	0.1576371075933186
>1k	3.175587528074161E-4	0.4328929380470306
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3001	0.18177049672831277	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2679	0.16226696459018658	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.056997558424284E-5
2	0.0	0.0	0.0	0.0	6.056997558424284E-5
3	0.0	0.0	0.0	0.0	6.056997558424284E-5
4	0.0	0.0	0.0	0.0	6.056997558424284E-5
5	0.0	0.0	0.0	0.0	6.056997558424284E-5
6	0.0	0.0	0.0	0.0	6.056997558424284E-5
7	0.0	0.0	0.0	0.0	6.056997558424284E-5
8	0.0	0.0	0.0	0.0	1.2113995116848568E-4
9	0.0	0.0	0.0	0.0	2.4227990233697137E-4
10-11	0.0	0.0	0.0	0.0	2.4227990233697137E-4
12-13	0.0	0.0	0.0	0.0	3.028498779212142E-4
14-15	0.0	0.0	0.0	6.056997558424284E-5	4.5427481688182125E-4
16-17	0.0	0.0	0.0	6.056997558424284E-5	5.451297802581855E-4
18-19	0.0	0.0	0.0	6.056997558424284E-5	6.056997558424284E-4
20-21	0.0	0.0	0.0	6.056997558424284E-5	7.874096825951569E-4
22-23	0.0	0.0	0.0	1.2113995116848568E-4	8.782646459715212E-4
24-25	0.0	0.0	0.0	2.4227990233697137E-4	9.085496337636426E-4
26-27	0.0	0.0	0.0	3.6341985350545704E-4	9.38834621555764E-4
28-29	0.0	0.0	0.0	0.0012113995116848568	9.691196093478855E-4
30-31	0.0	0.0	0.0	0.003937048412975785	9.994045971400069E-4
32-33	0.0	0.0	0.0	0.009479201178934006	0.001090259560516371
34-35	0.0	0.0	0.0	0.01705044812696436	0.001090259560516371
36-37	0.0	0.0	0.0	0.028467888524594136	0.001090259560516371
38-39	0.0	0.0	0.0	0.04476121195675546	0.001090259560516371
40-41	0.0	0.0	0.0	0.06665725813045925	0.001150829536100614
42-43	0.0	0.0	0.0	0.08703905491455696	0.0011811145238927352
44-45	0.0	0.0	0.0	0.11235730470877048	0.0012719694872690996
46-47	0.0	0.0	0.0	0.1401286385141458	0.0014233944262297066
48-49	0.0	0.0	0.0	0.16847538708757148	0.0015748193651903138
50-51	0.0	6.056997558424284E-5	0.0	0.200032344366962	0.0015748193651903138
52-53	0.0	6.056997558424284E-5	0.0	0.23110474184167856	0.0016353893407745567
54-55	0.0	6.056997558424284E-5	0.0	0.2655084879735285	0.001726244304150921
56-57	0.0	6.056997558424284E-5	0.0	0.30403099244510695	0.0017565292919430424
58-59	0.0	6.056997558424284E-5	0.0	0.34649054532966117	0.0017565292919430424
60-61	0.0	6.056997558424284E-5	0.0	0.39258429674927	0.0017565292919430424
62-63	0.0	6.056997558424284E-5	0.0	0.4441899159470449	0.001877669243111528
64-65	0.0	6.056997558424284E-5	0.0	0.49679493974195976	0.001877669243111528
66-67	0.0	6.056997558424284E-5	0.0	0.5555781010464674	0.001938239218695771
68-69	0.0	6.056997558424284E-5	0.0	0.6236587536031564	0.0020593791698642564
70-71	0.0	6.056997558424284E-5	0.0	0.7013094623021559	0.002180519121032742
72-73	0.0	6.056997558424284E-5	0.0	0.7812315450855642	0.0023319440599933496
74-75	0.0	6.056997558424284E-5	0.0	0.863485571928966	0.002392514035577592
76-77	0.0	1.2113995116848568E-4	0.0	0.9516754563796235	0.0024227990233697135
78-79	0.0	1.2113995116848568E-4	0.0	1.0567340790304929	0.002453084011161835
80-81	0.0	1.2113995116848568E-4	0.0	1.170514778165493	0.002513653986746078
82-83	0.0	1.2113995116848568E-4	0.0	1.293774678479427	0.0026045089501224423
84-85	0.0	1.2113995116848568E-4	0.0	1.4148237746845365	0.0026045089501224423
86-87	0.0	1.2113995116848568E-4	0.0	1.5495919703594767	0.0026347939379145636
88-89	0.0	1.2113995116848568E-4	0.0	1.7009866243322918	0.002665078925706685
90-91	0.0	1.2113995116848568E-4	0.0	1.8664940826162353	0.002695363913498806
92-93	0.0	1.2113995116848568E-4	0.0	2.0544427168541413	0.0027862188768751706
94-95	0.0	1.2113995116848568E-4	0.0	2.247933503858005	0.0028467888524594137
96-97	0.0	1.2113995116848568E-4	0.0	2.4514486218210605	0.0029679288036278994
98-99	0.0	1.2113995116848568E-4	0.0	2.675375821556006	0.003028498779212142
100-101	0.0	1.2113995116848568E-4	0.0	2.9251361158776317	0.003028498779212142
102-103	0.0	1.2113995116848568E-4	0.0	3.1992152553963304	0.003089068754796385
104-105	0.0	1.2113995116848568E-4	0.0	3.484378700446946	0.003089068754796385
106-107	0.0	1.2113995116848568E-4	0.0	3.777476812299097	0.003149638730380628
108-109	0.0	1.2113995116848568E-4	0.0	4.081356379805243	0.003240493693756992
110-111	0.0	1.2113995116848568E-4	0.0	4.420457388113627	0.0033616336449254778
112-113	0.0	1.2113995116848568E-4	0.0	4.78439208641155	0.003391918632717599
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2475	0.0	78.28845	1
GTATCAA	4995	0.0	61.708435	1
TCAACGC	6785	0.0	44.84837	4
CAACGCA	6915	0.0	44.063286	5
ATCAACG	7170	0.0	42.35714	3
AACGCAG	7305	0.0	41.859814	6
TATCAAC	7420	0.0	41.17078	2
ACGCAGA	8540	0.0	35.66591	7
GTACATG	8090	0.0	33.97363	1
CGCAGAG	9055	0.0	33.571728	8
GTGGTAT	1105	0.0	32.912216	1
TACATGG	8515	0.0	32.02996	2
GCAGAGT	9535	0.0	31.19445	9
ACATGGG	8690	0.0	31.04231	3
CATGGGG	6980	0.0	30.03052	4
AGAGTAC	8905	0.0	27.723127	10-11
TGGTATC	1185	0.0	26.633799	2
CAGAGTA	9215	0.0	26.37089	10-11
GAGTACT	5470	0.0	25.826462	12-13
GTACTTT	5685	0.0	24.851994	14-15
>>END_MODULE