FastQCFastQC Report
Mon 15 Aug 2016
SRR2921537_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921537_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817067
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.068.049061
GTATCAA39650.057.6318781
TCAACGC50950.043.5543754
CAACGCA51750.042.881075
AACGCAG54300.040.865076
ATCAACG54950.040.8180963
TATCAAC57400.039.696662
GTACATG89000.036.373381
ACGCAGA63800.034.591757
TACATGG94950.033.4590382
CGCAGAG66750.032.881128
ACATGGG97300.032.1626663
CATGGGG78750.031.2764114
GCAGAGT73900.028.8957169
AGAGTAC65650.026.36657110-11
CAGAGTA69900.025.10384610-11
ATGGGGG44200.024.9009885
ATGGGGA30550.024.342545
TGTACGC2802.6138878E-923.3723182
GTACGCT2102.7937167E-622.664693
Produced by FastQC (version 0.11.5)