##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921537_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1817067
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.862961024552206	31.0	30.0	34.0	25.0	34.0
2	29.89749910157413	31.0	30.0	34.0	25.0	34.0
3	30.053932518723855	31.0	30.0	34.0	25.0	34.0
4	33.430853677932625	37.0	35.0	37.0	27.0	37.0
5	33.457329311467326	37.0	35.0	37.0	27.0	37.0
6	33.45679108145159	37.0	35.0	37.0	27.0	37.0
7	33.33166746190427	37.0	35.0	37.0	26.0	37.0
8	33.254433105658734	37.0	35.0	37.0	26.0	37.0
9	34.724086123406565	39.0	35.0	39.0	27.0	39.0
10-11	34.609886702031346	39.0	35.0	39.0	26.0	39.0
12-13	34.47035772483899	39.0	35.0	39.0	25.0	39.0
14-15	35.62727296241691	40.0	36.0	41.0	25.0	41.0
16-17	35.55079449464439	40.0	36.0	41.0	25.0	41.0
18-19	35.561474893330846	40.0	36.0	41.0	25.0	41.0
20-21	35.59718601460486	40.0	36.0	41.0	25.0	41.0
22-23	35.560747072067244	40.0	36.0	41.0	25.0	41.0
24-25	35.52322313926784	40.0	35.5	41.0	25.0	41.0
26-27	35.448902544595214	40.0	35.0	41.0	25.0	41.0
28-29	35.40983986831526	40.0	35.0	41.0	25.0	41.0
30-31	35.317393910075964	40.0	35.0	41.0	25.0	41.0
32-33	35.22012699586752	40.0	35.0	41.0	24.5	41.0
34-35	35.05718171096608	40.0	35.0	41.0	24.0	41.0
36-37	34.914277239089145	39.5	35.0	41.0	24.0	41.0
38-39	34.790933685989565	39.0	34.0	41.0	23.0	41.0
40-41	34.64357065534733	39.0	34.0	41.0	22.0	41.0
42-43	34.45341200957367	39.0	34.0	41.0	20.5	41.0
44-45	34.2483664608955	39.0	33.5	41.0	19.5	41.0
46-47	34.00395114764618	39.0	33.0	41.0	18.0	41.0
48-49	33.862608258253545	38.0	33.0	41.0	16.0	41.0
50-51	33.667128674947044	38.0	33.0	40.0	15.0	41.0
52-53	33.4707253502485	38.0	33.0	40.0	13.5	41.0
54-55	33.268967242264594	38.0	33.0	40.0	12.5	41.0
56-57	33.02837677421911	37.0	32.5	40.0	10.0	41.0
58-59	32.78118445825058	37.0	32.0	40.0	8.0	41.0
60-61	32.47347731261423	36.5	32.0	40.0	7.5	41.0
62-63	32.15888627111714	36.0	31.5	39.0	7.0	41.0
64-65	31.85357281817346	35.0	31.0	39.0	4.0	41.0
66-67	31.81467799481252	35.0	31.0	39.0	2.0	41.0
68-69	31.571555149039632	35.0	31.0	38.5	2.0	40.5
70-71	31.274764496851247	35.0	31.0	37.5	2.0	40.0
72-73	30.94928723046536	35.0	31.0	37.0	2.0	39.0
74-75	30.649663165970217	35.0	31.0	36.5	2.0	39.0
76-77	30.372549278590167	35.0	31.0	36.0	2.0	39.0
78-79	30.101883144650145	35.0	30.5	35.5	2.0	37.0
80-81	29.8614608046924	35.0	30.0	35.0	2.0	37.0
82-83	29.66363210602581	35.0	30.0	35.0	2.0	36.5
84-85	29.458579127792206	35.0	30.0	35.0	2.0	36.0
86-87	29.30257002080826	34.5	30.0	35.0	2.0	36.0
88-89	29.176719955840923	34.0	30.0	35.0	2.0	35.5
90-91	29.06739597384136	34.0	30.0	35.0	2.0	35.0
92-93	28.96638126167059	34.0	30.0	35.0	2.0	35.0
94-95	28.896576460856977	34.0	30.0	35.0	2.0	35.0
96-97	28.794718356560324	34.0	29.0	35.0	2.0	35.0
98-99	28.73232439970568	34.0	29.0	35.0	2.0	35.0
100-101	28.667063184791754	34.0	29.0	35.0	2.0	35.0
102-103	27.679436421441807	32.5	27.5	34.5	2.0	35.0
104-105	28.511834181128158	34.0	29.0	35.0	2.0	35.0
106-107	28.834877305019575	34.5	30.0	35.0	2.0	35.0
108-109	28.87578636340872	35.0	30.0	35.0	2.0	35.0
110-111	28.81561274295334	35.0	30.0	35.0	2.0	35.0
112-113	28.746934758046898	35.0	30.0	35.0	2.0	35.0
114-115	28.62983946106555	35.0	30.0	35.0	2.0	35.0
116-117	28.56744412836731	35.0	30.0	35.0	2.0	35.0
118-119	28.409738606226405	34.5	30.0	35.0	2.0	35.0
120-121	28.236609877346297	34.0	29.5	35.0	2.0	35.0
122-123	28.11086245031141	34.0	29.0	35.0	2.0	35.0
124-125	27.261405055509783	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69507.0
3	12047.0
4	7961.0
5	6734.0
6	7086.0
7	8371.0
8	8973.0
9	9656.0
10	10088.0
11	10737.0
12	10550.0
13	11279.0
14	12284.0
15	10909.0
16	7982.0
17	7193.0
18	6460.0
19	6097.0
20	5697.0
21	6220.0
22	7342.0
23	9874.0
24	14071.0
25	19545.0
26	23186.0
27	24846.0
28	29117.0
29	34562.0
30	38986.0
31	46107.0
32	59066.0
33	75894.0
34	105562.0
35	164671.0
36	317495.0
37	473863.0
38	136881.0
39	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.01130872850849	16.973993007327937	12.13786277032469	24.876835493838882
2	16.707062844635914	19.254031314698796	38.33873447572954	25.700171364935752
3	19.28183459108522	24.088503749585076	28.65477889751244	27.97488276181726
4	13.38075776326394	15.091516451381334	35.539585929896106	35.98813985545862
5	14.505339755316497	36.995181310553605	33.342655448565324	15.156823485564578
6	33.79868444162547	34.44969148190543	17.11051758473305	14.641106491736053
7	29.85693954684218	30.98325501757631	20.57890346929169	18.580901966289815
8	27.722425610393902	33.679594814060394	19.250240854413832	19.347738721131876
9	27.632700943052946	14.845528356822035	18.81696427342645	38.70480642669857
10-11	26.28247529437413	24.963300436541065	26.855739954862223	21.898484314222586
12-13	26.59544365010069	22.563026644220002	26.95320112703102	23.88832857864829
14-15	24.041659544400687	23.726876032384496	24.30964942486498	27.921814998349838
16-17	24.239020956807224	26.6908129781194	24.77936762098549	24.290798444087883
18-19	24.28347254417214	25.960389606814267	26.248988266646112	23.507149582367486
20-21	25.417827777393835	24.92712570337773	26.009054667493036	23.645991851735396
22-23	25.20617686760933	24.598913550118564	25.817459790864866	24.377449791407237
24-25	24.57129750806136	24.98351382539551	25.966709354042077	24.47847931250105
26-27	24.268831090532334	25.102321113896814	26.082957529614415	24.545890265956434
28-29	24.408857468103175	25.059361333988544	26.074443586429414	24.457337611478867
30-31	24.70292564964883	25.038596815790225	25.83123556060636	24.427241973954583
32-33	24.315217938951157	25.180795569335	25.858825414542004	24.64516107717184
34-35	24.60474474224829	25.186420643810152	25.914898759324473	24.293935854617086
36-37	24.777703200115848	24.947512682026236	25.77024072311088	24.504543394747028
38-39	24.63122803027654	25.203421708282548	25.787151771193013	24.3781984902479
40-41	24.685935026204085	25.049050929551935	25.900047048148505	24.364966996095472
42-43	24.607434062991715	25.068686821408757	25.792049426428214	24.53182968917131
44-45	24.448743854320306	25.163965096036012	25.75426972791747	24.63302132172621
46-47	24.64340428718855	25.120765098941472	25.64129957123709	24.594531042632887
48-49	24.67422526812718	25.276083314200072	25.430415772634095	24.61927564503865
50-51	24.66467217697898	25.487805247359763	25.284052350811777	24.563470224849485
52-53	24.74512665198238	25.329873348017625	25.42128303964758	24.503716960352424
54-55	24.668403394796037	25.297391521709145	25.499098279670125	24.535106803824693
56-57	24.598794456349147	25.412437316795465	25.390469289909685	24.598298936945707
58-59	24.69423531575221	25.21230370441334	25.3987851999652	24.69467577986925
60-61	24.627461990622038	25.42873147012718	25.5358885324327	24.407918006818086
62-63	24.536855936092355	25.491594627234303	25.54092545401841	24.43062398265493
64-65	24.593405014329683	25.21600509157317	25.592202317359497	24.598387576737654
66-67	24.539122272671595	25.39915026679751	25.455689042240298	24.606038418290595
68-69	24.51030584484146	25.39723826919499	25.4988323969388	24.59362348902475
70-71	24.462607514974255	25.133843978129068	25.68488446071896	24.71866404617772
72-73	24.59854645465736	25.34144883304597	25.44764065875265	24.612364053544024
74-75	24.410539502765243	25.577307240120557	25.416988849558642	24.595164407555554
76-77	24.46171623229989	25.265756278234026	25.505693872989692	24.766833616476394
78-79	24.42600610127858	25.298973935930245	25.57469579623943	24.70032416655175
80-81	24.508225124190858	25.566926548425833	25.292466750098185	24.63238157728512
82-83	24.62951964565549	25.404029034056723	25.360985444992	24.60546587529579
84-85	24.454621864003474	25.488078548322378	25.325757237994743	24.7315423496794
86-87	24.258551658922205	25.54687353793686	25.512802306495768	24.68177249664517
88-89	24.34986156141114	25.383884977624138	25.487149706334044	24.779103754630675
90-91	24.32718118127264	25.45965013476466	25.41528815199318	24.797880531969525
92-93	24.454195102390624	25.59527528239402	25.339432891874942	24.611096723340417
94-95	24.360267465345615	25.330829710042224	25.493117558653562	24.815785265958603
96-97	24.413643737676317	25.282909827437454	25.52103815474626	24.782408280139965
98-99	24.320724487161918	25.38124023404713	25.42428060117816	24.873754677612787
100-101	24.319920480004008	25.397056472241342	25.521086310953017	24.761936736801637
102-103	24.29062552198839	25.213810570947633	25.646921620356522	24.848642286707463
104-105	24.14161280176294	25.48935218194482	25.47630847755478	24.892726538737463
106-107	24.178286962226515	25.409893358929537	25.511025154435817	24.900794524408123
108-109	24.190593085304002	25.375828010490654	25.496200852572166	24.93737805163318
110-111	24.21713479659883	25.418067011252564	25.550293112657464	24.814505079491138
112-113	24.154210756314733	25.45560067409967	25.61955747624305	24.77063109334255
114-115	24.015265259755985	25.633208874720957	25.6109186014257	24.74060726409736
116-117	23.952299313962097	25.638334466181057	25.519674400301607	24.889691819555246
118-119	24.138485539553976	25.381660228891324	25.44010772654405	25.03974650501065
120-121	23.89213227722313	25.438658261748202	25.661097538311058	25.008111922717607
122-123	23.92634507572588	25.555310280135114	25.554126719191082	24.964217924947924
124-125	23.88108453196331	25.545609395681357	25.624172745809727	24.949133326545606
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	132.0
1	150.0
2	171.5
3	179.0
4	169.5
5	154.5
6	169.5
7	221.5
8	308.0
9	355.5
10	377.0
11	418.0
12	489.0
13	575.0
14	635.5
15	715.0
16	778.0
17	865.0
18	968.0
19	1065.5
20	1246.0
21	1441.0
22	1700.5
23	2121.5
24	2578.5
25	3305.5
26	4517.5
27	5760.0
28	6778.0
29	8426.5
30	10690.5
31	12769.0
32	15417.5
33	18291.5
34	20974.0
35	23882.0
36	27734.0
37	31564.5
38	33750.0
39	36369.5
40	39320.0
41	41846.0
42	44455.5
43	49010.0
44	53573.5
45	57017.5
46	61254.0
47	64882.5
48	69067.5
49	73283.0
50	74843.5
51	75592.5
52	75512.5
53	75237.0
54	76293.0
55	75862.0
56	72213.0
57	66572.0
58	62271.0
59	57804.0
60	51619.5
61	45628.0
62	39297.5
63	31056.5
64	23317.5
65	18154.5
66	13885.5
67	10576.0
68	8449.5
69	6701.5
70	5598.0
71	4546.5
72	3600.0
73	3101.5
74	2415.0
75	1798.0
76	1253.0
77	719.0
78	434.0
79	293.5
80	167.5
81	77.5
82	54.0
83	40.5
84	28.0
85	21.0
86	17.0
87	13.5
88	13.5
89	14.0
90	11.0
91	11.0
92	9.5
93	10.0
94	10.5
95	6.5
96	5.0
97	4.5
98	8.0
99	10.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03269004390041754
2	0.02619606211548611
3	0.02564572467608514
4	0.03098399783827454
5	0.03291017887617793
6	0.04424713012783788
7	0.038633688245948004
8	0.03395582001103977
9	0.028617546848850376
10-11	0.03458870806635088
12-13	0.03373568503527938
14-15	0.03194708835722623
16-17	0.035249112993632044
18-19	0.04826459343546496
20-21	0.06039953397425631
22-23	0.05330018100598382
24-25	0.06337135614702154
26-27	0.04669613173317219
28-29	0.04730150291651326
30-31	0.04705385106878282
32-33	0.05484112583630653
34-35	0.037285361519415625
36-37	0.05030084196124854
38-39	0.05907872411969399
40-41	0.04636592926953161
42-43	0.05693240810603021
44-45	0.05808811672877225
46-47	0.06301363681141092
48-49	0.04721895230060311
50-51	0.049337751442296844
52-53	0.05872100478408336
54-55	0.0449350519270891
56-57	0.043531691456616624
58-59	0.04408202889601759
60-61	0.03161688589358565
62-63	0.04174309477856347
64-65	0.040312217436120956
66-67	0.03381823565118953
68-69	0.028892715568550855
70-71	0.04768673912409394
72-73	0.029883322959472602
74-75	0.03750549649517602
76-77	0.03043366039887357
78-79	0.03095648096630449
80-81	0.022426250655589477
82-83	0.01673025815778945
84-85	0.01651012318202906
86-87	0.01568461702292761
88-89	0.02074772146541652
90-91	0.010786613812258986
92-93	0.017803416164621336
94-95	0.012850379210012619
96-97	0.010428894476648357
98-99	0.009245668981936274
100-101	0.009108084622086033
102-103	0.0053107562902193475
104-105	0.005420823778099541
106-107	0.0072094204561526905
108-109	0.011667153715300537
110-111	0.0056959924978000264
112-113	0.006879217992512108
114-115	0.0072094204561526905
116-117	0.006163779321290849
118-119	0.23824107751667933
120-121	0.35554550272499585
122-123	0.028342378129149887
124-125	0.0034120921242860058
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1817067.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.72703295747783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.02480684030098	48.39371308523931
2	9.874792348208784	11.79584096059432
3	3.3732587930281874	6.044242173158892
4	1.6398640984765325	3.917768682219701
5	0.9193545915855358	2.7455160995618932
6	0.6453886231828304	2.312828854033329
7	0.4338630146044796	1.8139345400618705
8	0.3198996447369387	1.528532529943087
9	0.2607060992174452	1.401408160215825
>10	1.4150731652527724	14.93458340161333
>50	0.07206383054373772	2.8995452332917355
>100	0.020558634172036527	1.9804563929597396
>500	1.8515834481704576E-4	0.08238924129613603
>1k	1.8515834481704576E-4	0.14924064581092025
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	5.503374394009687E-5
10-11	0.0	0.0	0.0	0.0	8.25506159101453E-5
12-13	0.0	0.0	0.0	0.0	2.2013497576038748E-4
14-15	0.0	0.0	0.0	0.0	3.026855916705328E-4
16-17	0.0	0.0	0.0	0.0	3.302024636405812E-4
18-19	0.0	0.0	0.0	0.0	3.302024636405812E-4
20-21	0.0	0.0	0.0	0.0	3.302024636405812E-4
22-23	0.0	0.0	0.0	0.0	3.577193356106296E-4
24-25	0.0	0.0	0.0	1.1006748788019374E-4	3.852362075806781E-4
26-27	0.0	0.0	0.0	2.2013497576038748E-4	3.852362075806781E-4
28-29	0.0	0.0	0.0	8.530230310715014E-4	3.852362075806781E-4
30-31	0.0	0.0	0.0	0.0034396089962560545	3.852362075806781E-4
32-33	0.0	0.0	0.0	0.007759757895553659	3.852362075806781E-4
34-35	0.0	0.0	0.0	0.012932929825922763	4.4026995152077496E-4
36-37	0.0	0.0	0.0	0.020500069617686086	4.4026995152077496E-4
38-39	0.0	0.0	0.0	0.033763201907249435	4.4026995152077496E-4
40-41	0.0	0.0	0.0	0.04972298764987752	4.4026995152077496E-4
42-43	0.0	0.0	0.0	0.06460961538567372	4.6778682349082337E-4
44-45	0.0	0.0	0.0	0.08334860519727672	5.503374394009687E-4
46-47	0.0	0.0	0.0	0.10437149538239371	6.053711833410656E-4
48-49	0.0	0.0	0.0	0.12745815096526436	6.604049272811624E-4
50-51	0.0	0.0	0.0	0.15233340322618816	7.154386712212593E-4
52-53	0.0	0.0	0.0	0.17709858799923173	7.704724151613562E-4
54-55	0.0	0.0	0.0	0.20376243693820867	7.704724151613562E-4
56-57	0.0	0.0	0.0	0.23727798699772767	7.704724151613562E-4
58-59	0.0	0.0	0.0	0.27720496822626794	7.704724151613562E-4
60-61	0.0	0.0	0.0	0.32051652470712416	7.704724151613562E-4
62-63	0.0	0.0	0.0	0.36481868857890215	8.255061591014531E-4
64-65	0.0	0.0	0.0	0.4103040779453922	8.530230310715016E-4
66-67	0.0	0.0	0.0	0.46010961621117985	9.355736469816467E-4
68-69	0.0	0.0	0.0	0.5188306209952633	9.906073909217437E-4
70-71	0.0	0.0	0.0	0.5836878882286674	0.0010456411348618406
72-73	0.0	0.0	0.0	0.6517646294825672	0.001073158006831889
74-75	0.0	0.0	0.0	0.7210521131031492	0.0011006748788019373
76-77	0.0	0.0	0.0	0.7999705019132481	0.0011006748788019373
78-79	0.0	0.0	0.0	0.8895929539196958	0.0011281917507719858
80-81	0.0	0.0	0.0	0.9885436255239901	0.0011832254947120827
82-83	0.0	0.0	0.0	1.0944010319927662	0.0013758435985024217
84-85	0.0	0.0	0.0	1.2033678449941583	0.0013758435985024217
86-87	0.0	0.0	0.0	1.3158568176077163	0.0014033604704724702
88-89	0.0	0.0	0.0	1.4512123108283843	0.0014308773424425187
90-91	0.0	0.0	0.0	1.597987305916623	0.0014308773424425187
92-93	0.0	0.0	0.0	1.7632536389687337	0.0014859110863826156
94-95	0.0	0.0	0.0	1.9341884476466746	0.0014859110863826156
96-97	0.0	0.0	0.0	2.117285713735377	0.0016234954462328575
98-99	0.0	0.0	0.0	2.3159575293591264	0.0017335629341130514
100-101	0.0	0.0	0.0	2.543797229271128	0.0017610798060830998
102-103	0.0	0.0	0.0	2.7910088070500425	0.0017885966780531483
104-105	0.0	0.0	0.0	3.0440539616866085	0.0018161135500231968
106-107	0.0	0.0	0.0	3.3048038404747873	0.0018711472939632937
108-109	0.0	0.0	0.0	3.581816190597265	0.0019261810379033904
110-111	0.0	0.0	0.0	3.88582259212236	0.0020637653977536325
112-113	0.0	0.0	0.0	4.211567322503793	0.002146316013663778
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2125	0.0	68.04906	1
GTATCAA	3965	0.0	57.631878	1
TCAACGC	5095	0.0	43.554375	4
CAACGCA	5175	0.0	42.88107	5
AACGCAG	5430	0.0	40.86507	6
ATCAACG	5495	0.0	40.818096	3
TATCAAC	5740	0.0	39.69666	2
GTACATG	8900	0.0	36.37338	1
ACGCAGA	6380	0.0	34.59175	7
TACATGG	9495	0.0	33.459038	2
CGCAGAG	6675	0.0	32.88112	8
ACATGGG	9730	0.0	32.162666	3
CATGGGG	7875	0.0	31.276411	4
GCAGAGT	7390	0.0	28.895716	9
AGAGTAC	6565	0.0	26.366571	10-11
CAGAGTA	6990	0.0	25.103846	10-11
ATGGGGG	4420	0.0	24.900988	5
ATGGGGA	3055	0.0	24.34254	5
TGTACGC	280	2.6138878E-9	23.372318	2
GTACGCT	210	2.7937167E-6	22.66469	3
>>END_MODULE