FastQCFastQC Report
Mon 15 Aug 2016
SRR2921537_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921537_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817067
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32790.18045564637957764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28980.15948778993840074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26550.076.794151
GTATCAA54350.061.865161
TCAACGC71550.046.8479734
CAACGCA74000.045.5961535
TATCAAC73900.045.5218662
ATCAACG76650.043.8085633
AACGCAG77500.043.6797186
ACGCAGA90200.037.1999057
CGCAGAG95150.035.388738
GTACATG90150.032.932921
GCAGAGT102100.032.5710269
ACATGGG95000.030.646253
TACATGG96500.030.480052
TACCGTA1403.098361E-629.746737
CATGGGG77750.027.9501764
GTGGTAT11450.027.0747491
CAGAGTA100300.026.72017110-11
AGAGTAC96100.026.6498710-11
GAGTACT59200.024.01781712-13
ATGGGGG41900.023.4340175
Produced by FastQC (version 0.11.5)