##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921537_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1817067
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32554715924069	31.0	31.0	34.0	28.0	34.0
2	31.38104593831708	33.0	31.0	34.0	28.0	34.0
3	31.45965118512416	33.0	31.0	34.0	28.0	34.0
4	34.98928713140462	37.0	35.0	37.0	32.0	37.0
5	34.66107303693259	37.0	35.0	37.0	32.0	37.0
6	34.64676206215841	37.0	35.0	37.0	31.0	37.0
7	34.500057510262415	37.0	35.0	37.0	30.0	37.0
8	34.467732340084325	37.0	35.0	37.0	30.0	37.0
9	36.04964979277044	39.0	37.0	39.0	30.0	39.0
10-11	35.928099514217145	39.0	37.0	39.0	30.0	39.0
12-13	35.83888541259073	39.0	37.0	39.0	30.0	39.0
14-15	37.02951707339355	40.0	37.0	41.0	30.0	41.0
16-17	37.011037567684625	40.0	37.0	41.0	30.0	41.0
18-19	37.03637510339465	40.0	37.0	41.0	30.0	41.0
20-21	37.02092740663938	40.0	37.0	41.0	30.0	41.0
22-23	36.96320361329549	40.0	37.0	41.0	30.0	41.0
24-25	36.905750861140504	40.0	37.0	41.0	30.0	41.0
26-27	36.783017907429944	40.0	36.5	41.0	30.0	41.0
28-29	36.595839889228074	40.0	36.0	41.0	30.0	41.0
30-31	36.54741569793519	40.0	36.0	41.0	30.0	41.0
32-33	36.41982959351526	40.0	36.0	41.0	30.0	41.0
34-35	36.30138321812019	40.0	35.0	41.0	29.0	41.0
36-37	36.17633306862103	40.0	35.0	41.0	29.0	41.0
38-39	36.00765932131286	40.0	35.0	41.0	28.0	41.0
40-41	35.80820547618772	40.0	35.0	41.0	27.0	41.0
42-43	35.633165975718015	39.5	35.0	41.0	27.0	41.0
44-45	35.41509256400562	39.0	35.0	41.0	26.0	41.0
46-47	35.13360376915105	39.0	35.0	41.0	24.5	41.0
48-49	34.866129592359556	39.0	34.5	41.0	24.0	41.0
50-51	34.648236691327284	39.0	34.0	41.0	23.0	41.0
52-53	34.445798641436994	38.5	34.0	41.0	23.0	41.0
54-55	34.23696814701934	38.0	34.0	40.0	22.5	41.0
56-57	33.9762270736302	38.0	33.5	40.0	22.0	41.0
58-59	33.824985539883784	37.0	33.5	40.0	22.0	41.0
60-61	33.70821906952248	37.0	33.0	40.0	22.0	41.0
62-63	33.44073498665707	36.5	33.0	40.0	22.0	41.0
64-65	33.14511545253973	36.0	33.0	39.5	20.5	41.0
66-67	32.84250388125479	35.5	33.0	39.0	20.0	41.0
68-69	32.525331481998194	35.0	33.0	39.0	20.0	41.0
70-71	32.22027696281975	35.0	32.0	37.5	19.5	40.0
72-73	31.908703696671616	35.0	32.0	37.0	18.0	39.5
74-75	31.620337885174294	35.0	32.0	36.5	18.0	39.0
76-77	31.325133580654978	35.0	32.0	36.0	17.0	39.0
78-79	31.05015940523932	35.0	32.0	36.0	15.0	37.5
80-81	30.820880572923286	35.0	32.0	35.0	13.0	37.0
82-83	30.632450537046793	35.0	31.0	35.0	11.5	37.0
84-85	30.441583882157346	35.0	31.0	35.0	10.5	36.0
86-87	30.29685586717496	35.0	31.0	35.0	9.0	36.0
88-89	30.184283793608053	35.0	31.0	35.0	7.0	36.0
90-91	30.08700339613234	35.0	31.0	35.0	7.0	35.0
92-93	30.14740705763739	35.0	31.0	35.0	7.0	35.0
94-95	30.116836088047386	35.0	31.0	35.0	7.0	35.0
96-97	30.094353978141697	35.0	31.0	35.0	6.0	35.0
98-99	30.051945250230176	35.0	31.0	35.0	2.0	35.0
100-101	30.001972134214093	35.0	31.0	35.0	2.0	35.0
102-103	29.121980092093466	33.5	29.5	35.0	2.0	35.0
104-105	29.828331591515337	34.5	31.0	35.0	2.0	35.0
106-107	29.93464495255266	35.0	31.0	35.0	2.0	35.0
108-109	29.94202332660271	35.0	32.0	35.0	2.0	35.0
110-111	29.910941643868938	35.0	32.0	35.0	2.0	35.0
112-113	29.869033723027275	35.0	32.0	35.0	2.0	35.0
114-115	29.80454435637211	35.0	31.0	35.0	2.0	35.0
116-117	29.73014368760205	35.0	31.0	35.0	2.0	35.0
118-119	29.63855102756255	35.0	31.0	35.0	2.0	35.0
120-121	29.518672949318876	35.0	31.0	35.0	2.0	35.0
122-123	29.40224878884488	35.0	31.0	35.0	2.0	35.0
124-125	28.500365148891042	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26546.0
3	9896.0
4	5644.0
5	4805.0
6	5103.0
7	6148.0
8	7279.0
9	7891.0
10	9330.0
11	10194.0
12	10840.0
13	12693.0
14	16438.0
15	13716.0
16	7614.0
17	6383.0
18	5748.0
19	5215.0
20	4973.0
21	5432.0
22	6459.0
23	8416.0
24	11682.0
25	16516.0
26	21046.0
27	23151.0
28	26983.0
29	32819.0
30	37579.0
31	44828.0
32	57059.0
33	72754.0
34	100852.0
35	159453.0
36	321057.0
37	523632.0
38	170446.0
39	447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.139400457643205	17.55926193048738	12.521715462290716	24.779622149578696
2	17.037473239297093	19.657683142270628	37.55606676829772	25.748776850134558
3	19.46050711494499	24.146556142037984	28.82456848220562	27.56836826081141
4	13.495221439317016	15.56220498802882	35.206780952716585	35.73579261993758
5	14.398642284599475	37.01959890585666	33.31747937471547	15.264279434828396
6	33.880699135410836	34.86583648052156	16.453615647263913	14.799848736803689
7	29.61310269172996	31.311540648408425	20.342670879965567	18.732685779896048
8	27.945923366118556	33.478134285684405	18.93535251134164	19.640589836855394
9	27.601840834517517	14.47846507598781	18.74422812963332	39.175465959861356
10-11	26.44131397519297	24.88834964414158	26.567845082774955	22.1024912978905
12-13	26.897243849859464	22.533215629576592	27.027734314589342	23.541806205974602
14-15	24.210416245303083	23.488684066058802	24.727472355109402	27.57342733352871
16-17	24.086652561963092	26.522465886724994	25.120728410336795	24.27015314097512
18-19	24.077098537760733	25.33308823711518	27.304691015031313	23.285122210092773
20-21	25.14138535910918	24.318089124927138	27.228329580486594	23.31219593547709
22-23	24.87392816581683	24.009856237518374	27.101626075549262	24.014589521115536
24-25	24.316004191930535	24.49717198804067	27.153290577403205	24.033533242625595
26-27	24.051520140809682	24.559990379325896	27.23847851350678	24.150010966357634
28-29	24.16017802450809	24.563187994904307	27.283092487815995	23.99354149277161
30-31	24.3614732793081	24.53015992892537	27.08085399646168	24.027512795304848
32-33	24.05596002933677	24.66991315930335	27.026514552111987	24.247612259247894
34-35	24.240826269454622	24.67166346809358	27.09378098861367	23.99372927383813
36-37	24.48057137698948	24.4588023804439	26.87089526443139	24.18973097813523
38-39	24.407333932953378	24.72648298623993	26.811850679196304	24.054332401610388
40-41	24.42303495597858	24.611625464149228	26.912523229820913	24.05281635005128
42-43	24.422685465089238	24.692867153881654	26.616831565190758	24.267615815838347
44-45	24.40287963028709	24.780997610582787	26.52566848363654	24.290454275493584
46-47	24.581899484144547	24.811948339597134	26.210432917874844	24.395719258383476
48-49	24.717605632639895	25.08844347736579	25.744771968027692	24.449178921966624
50-51	24.62021939057318	25.353622058057397	25.60604313450174	24.42011541686768
52-53	24.80436862367767	25.204934050097926	25.603875637012973	24.38682168921143
54-55	24.658865452587932	25.224182387574423	25.712411900580616	24.404540259257026
56-57	24.58352966043851	25.326196567835236	25.57194489336686	24.518328878359398
58-59	24.569538720323738	25.068500615377165	25.794816300559454	24.567144363739644
60-61	24.633500576590166	25.203512934059553	25.858183589610917	24.304802899739364
62-63	24.506913246510763	25.303387720603393	25.892947819761563	24.29675121312428
64-65	24.57338259678962	25.030156666047432	25.867029950254917	24.52943078690803
66-67	24.55030925139834	25.21094454114523	25.815439866254792	24.42330634120164
68-69	24.57328631260199	25.195229338910647	25.705758223054527	24.525726125432836
70-71	24.414228538042018	25.040599929491936	25.93738983115218	24.607781701313865
72-73	24.683006762595788	25.27749981010129	25.571952896313103	24.46754053098982
74-75	24.53215232361296	25.48365887335658	25.4714117321008	24.512777070929662
76-77	24.501835736720594	25.200877014583796	25.57881228756105	24.71847496113456
78-79	24.53157423481444	25.222531999769892	25.591062992678648	24.654830772737018
80-81	24.530296996240175	25.429396548094036	25.396534883317695	24.64377157234809
82-83	24.6584680092475	25.308908162616646	25.435853518323693	24.596770309812165
84-85	24.490095830115788	25.396301643328794	25.332207582198084	24.78139494435733
86-87	24.38696634754572	25.43098940703387	25.518530260349415	24.663513985070995
88-89	24.413230432780658	25.27916605085414	25.46678589347586	24.840817622889347
90-91	24.360332364354687	25.301928628021454	25.530069298072526	24.807669709551334
92-93	24.44129443019875	25.47667127335611	25.408781770058496	24.673252526386644
94-95	24.434000764471502	25.17731033167551	25.53175212300451	24.856936780848475
96-97	24.396336661289762	25.164705500346447	25.580053394796238	24.858904443567557
98-99	24.38320753818776	25.184510095118906	25.442649493696205	24.989632872997124
100-101	24.309335705172728	25.197702207197636	25.566302341274078	24.926659746355554
102-103	24.336480436320006	25.069660954917815	25.687212268473292	24.90664634028888
104-105	24.21194071414459	25.301770419481933	25.473617067880667	25.012671798492814
106-107	24.272686197451602	25.26417631089291	25.45921632876791	25.003921162887583
108-109	24.315263598538245	25.151880778611574	25.44072535078309	25.09213027206709
110-111	24.307885519371006	25.221615044314756	25.452730333118055	25.01776910319618
112-113	24.263822437874225	25.172613638802833	25.535838416572233	25.027725506750713
114-115	24.089449167653623	25.352883163312477	25.497404558642884	25.060263110391013
116-117	24.03780769940969	25.39622434857891	25.451702499516355	25.114265452495044
118-119	24.140834791395715	25.103458676246305	25.45003629791035	25.305670234447625
120-121	23.89676189396443	25.189302056930547	25.661095314345843	25.25284073475918
122-123	23.908924306296985	25.235490297513564	25.541891847269767	25.313693548919687
124-125	23.925816729383325	25.29807842627802	25.503953461232744	25.272151383105918
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	40.0
1	40.0
2	78.0
3	125.5
4	101.5
5	83.5
6	154.5
7	346.0
8	598.5
9	707.5
10	616.5
11	516.5
12	557.5
13	680.0
14	828.5
15	950.5
16	1116.5
17	1341.0
18	1564.0
19	1789.0
20	1964.5
21	2177.0
22	2530.0
23	2976.5
24	3427.0
25	4072.0
26	5167.5
27	6410.5
28	7452.5
29	8945.5
30	11043.5
31	13147.5
32	15710.5
33	18290.0
34	20896.5
35	23762.0
36	27265.5
37	30945.0
38	33243.0
39	35681.0
40	38479.0
41	41042.0
42	43768.5
43	48124.0
44	52647.0
45	56356.5
46	61015.5
47	64841.0
48	69101.5
49	73323.5
50	74214.5
51	74934.0
52	75156.0
53	75371.5
54	76620.5
55	75730.5
56	72045.5
57	66510.5
58	62707.0
59	58473.5
60	51665.0
61	45464.5
62	39090.5
63	30725.0
64	23022.5
65	17693.0
66	13569.0
67	10431.0
68	8202.0
69	6482.5
70	5406.5
71	4380.0
72	3391.5
73	2954.0
74	2355.5
75	1735.0
76	1183.5
77	662.5
78	383.5
79	236.0
80	126.5
81	47.5
82	24.5
83	12.0
84	9.0
85	9.5
86	7.5
87	5.0
88	3.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11645140217724496
2	0.0020362485257835843
3	0.021903430088158555
4	0.057180059953760645
5	0.026746399554887078
6	0.020142350282075453
7	0.015409448303227123
8	0.004953036954608718
9	0.003577193356106297
10-11	0.006741633632661866
12-13	0.004182564539447362
14-15	0.0027241703250347952
16-17	0.009851040165277339
18-19	0.005145655058399057
20-21	0.012327558642581699
22-23	0.008062443487224192
24-25	0.013373199777443539
26-27	0.008062443487224192
28-29	0.013125547929713103
30-31	0.021187991416937297
32-33	0.012905412953952717
34-35	0.02044503587374599
36-37	0.014473874656245476
38-39	0.01986718156237497
40-41	0.009218152109966226
42-43	0.007787274767523707
44-45	0.016840325645669642
46-47	0.015354414559287027
48-49	0.020362485257835843
50-51	0.014391324040335333
52-53	0.021187991416937297
54-55	0.016427572566118914
56-57	0.02041751900177594
58-59	0.016234954462328578
60-61	0.0193718778669141
62-63	0.020555103361626182
64-65	0.016757775029759496
66-67	0.009878557037247389
68-69	0.02319672307075083
70-71	0.013951054088814557
72-73	0.017005426877489932
74-75	0.01741817995704066
76-77	0.012189974282731457
78-79	0.02919540116022139
80-81	0.019839664690404924
82-83	0.007897342255403901
84-85	0.011336951251659956
86-87	0.01106178253195947
88-89	0.019894698434345017
90-91	0.011309434379689906
92-93	0.00792485912737395
94-95	0.006906734864482157
96-97	0.0019812147818434873
98-99	0.0014033604704724702
100-101	0.010621512580438695
102-103	0.0028617546848850373
104-105	0.002201349757603875
106-107	2.2013497576038748E-4
108-109	0.019041675403273516
110-111	4.6778682349082337E-4
112-113	0.008144994103134337
114-115	0.0019261810379033904
116-117	0.007457072303883126
118-119	0.008695331542535305
120-121	0.0034946427401961514
122-123	3.852362075806781E-4
124-125	0.02371954363818175
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1817067.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.016624888749
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.19387937260626	46.52578217934084
2	10.204822740365168	11.840987459678832
3	3.5118765470039572	6.11241672849371
4	1.7455971521791045	4.050946207393745
5	1.0142679991589612	2.942220302193372
6	0.6648230341930915	2.3142473155308325
7	0.4735726363190441	1.9232560199228552
8	0.3440029676680107	1.5966312908649143
9	0.2639684665021386	1.3783103553161595
>10	1.4768483038982159	15.214439386803694
>50	0.08113600075447798	3.1651118799020264
>100	0.024729331837886812	2.4258459731116178
>500	1.9017900543558545E-4	0.08054487134652354
>1k	2.852685081533782E-4	0.42926003010089026
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3279	0.18045564637957764	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2898	0.15948778993840074	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	5.503374394009687E-5
5	0.0	0.0	0.0	0.0	5.503374394009687E-5
6	0.0	0.0	0.0	0.0	1.1006748788019374E-4
7	0.0	0.0	0.0	0.0	1.1006748788019374E-4
8	0.0	0.0	0.0	0.0	1.1006748788019374E-4
9	0.0	0.0	0.0	0.0	1.1006748788019374E-4
10-11	0.0	0.0	0.0	0.0	1.1006748788019374E-4
12-13	0.0	0.0	0.0	0.0	2.2013497576038748E-4
14-15	0.0	0.0	0.0	0.0	2.2013497576038748E-4
16-17	0.0	0.0	0.0	0.0	2.476518477304359E-4
18-19	0.0	0.0	0.0	0.0	2.751687197004843E-4
20-21	0.0	0.0	0.0	0.0	2.751687197004843E-4
22-23	0.0	0.0	0.0	0.0	2.751687197004843E-4
24-25	0.0	0.0	0.0	1.1006748788019374E-4	2.751687197004843E-4
26-27	0.0	0.0	0.0	1.651012318202906E-4	3.302024636405812E-4
28-29	0.0	0.0	0.0	7.429555431913078E-4	3.302024636405812E-4
30-31	0.0	0.0	0.0	0.0033295415083758606	3.302024636405812E-4
32-33	0.0	0.0	0.0	0.007677207279643513	3.302024636405812E-4
34-35	0.0	0.0	0.0	0.012767828594102474	3.302024636405812E-4
36-37	0.0	0.0	0.0	0.020362485257835843	3.302024636405812E-4
38-39	0.0	0.0	0.0	0.033955820011039764	3.577193356106296E-4
40-41	0.0	0.0	0.0	0.05024580821730844	3.852362075806781E-4
42-43	0.0	0.0	0.0	0.06518746969704474	4.1275307955072654E-4
44-45	0.0	0.0	0.0	0.08403652699652792	4.6778682349082337E-4
46-47	0.0	0.0	0.0	0.10527955215740531	5.778543113710171E-4
48-49	0.0	0.0	0.0	0.12899909579558708	6.053711833410656E-4
50-51	0.0	0.0	0.0	0.154507236111822	7.154386712212593E-4
52-53	0.0	0.0	0.0	0.17965765709244624	8.255061591014531E-4
54-55	0.0	0.0	0.0	0.20709197844658453	0.0010181242628917921
56-57	0.0	0.0	0.0	0.24132296717732477	0.0010456411348618406
58-59	0.0	0.0	0.0	0.28213048830890664	0.0010456411348618406
60-61	0.0	0.0	0.0	0.3267078209003851	0.0011006748788019373
62-63	0.0	0.0	0.0	0.37175294031535433	0.0011557086227420342
64-65	0.0	0.0	0.0	0.41828397081670626	0.0012382592386521796
66-67	0.0	0.0	0.0	0.4693002514491761	0.001265776110622228
68-69	0.0	0.0	0.0	0.5293145492158517	0.001265776110622228
70-71	0.0	0.0	0.0	0.5952449744560877	0.001265776110622228
72-73	0.0	0.0	0.0	0.6648351436683402	0.0013208098545623248
74-75	0.0	0.0	0.0	0.735168268423784	0.0013208098545623248
76-77	0.0	0.0	0.0	0.8150772646248047	0.0013483267265323733
78-79	0.0	0.0	0.0	0.9057178408941442	0.0013758435985024217
80-81	0.0	0.0	0.0	1.0057691873772403	0.0014033604704724702
82-83	0.0	0.0	0.0	1.1139380110915007	0.0014859110863826156
84-85	0.0	0.0	0.0	1.2241706002035149	0.0014859110863826156
86-87	0.0	0.0	0.0	1.339713945605748	0.0016234954462328577
88-89	0.0	0.0	0.0	1.4776560247916009	0.0017610798060830998
90-91	0.0	0.0	0.0	1.6278155951321551	0.0017610798060830998
92-93	0.0	0.0	0.0	1.795806098509301	0.0019261810379033904
94-95	0.0	0.0	0.0	1.9701254824395575	0.0020362485257835843
96-97	0.0	0.0	0.0	2.157212694963917	0.002091282269723681
98-99	0.0	0.0	0.0	2.3597643895354437	0.002146316013663778
100-101	0.0	0.0	0.0	2.5909611478277905	0.002146316013663778
102-103	0.0	0.0	0.0	2.8419975708105425	0.0022563835015439715
104-105	0.0	0.0	0.0	3.0988400537789746	0.0023389341174541167
106-107	0.0	0.0	0.0	3.3647355876255527	0.002393967861394214
108-109	0.0	0.0	0.0	3.6487097063564526	0.0024490016053343106
110-111	0.0	0.0	0.0	3.9607510344968015	0.0026141028371546014
112-113	0.0	0.0	0.0	4.295548815756381	0.0027516871970048435
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2655	0.0	76.79415	1
GTATCAA	5435	0.0	61.86516	1
TCAACGC	7155	0.0	46.847973	4
CAACGCA	7400	0.0	45.596153	5
TATCAAC	7390	0.0	45.521866	2
ATCAACG	7665	0.0	43.808563	3
AACGCAG	7750	0.0	43.679718	6
ACGCAGA	9020	0.0	37.199905	7
CGCAGAG	9515	0.0	35.38873	8
GTACATG	9015	0.0	32.93292	1
GCAGAGT	10210	0.0	32.571026	9
ACATGGG	9500	0.0	30.64625	3
TACATGG	9650	0.0	30.48005	2
TACCGTA	140	3.098361E-6	29.74673	7
CATGGGG	7775	0.0	27.950176	4
GTGGTAT	1145	0.0	27.074749	1
CAGAGTA	10030	0.0	26.720171	10-11
AGAGTAC	9610	0.0	26.64987	10-11
GAGTACT	5920	0.0	24.017817	12-13
ATGGGGG	4190	0.0	23.434017	5
>>END_MODULE