FastQCFastQC Report
Mon 15 Aug 2016
SRR2921536_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921536_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071377
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22450.068.127031
GTATCAA42500.059.7917981
TCAACGC53950.047.1985934
CAACGCA56350.045.3995255
ATCAACG59200.043.2107773
AACGCAG59900.042.605466
TATCAAC64150.039.4118732
GTACATG110600.038.1499331
TACATGG114200.036.4121132
ACGCAGA71000.035.9446077
ACATGGG115950.035.5042723
CGCAGAG75650.033.6557438
CATGGGG97100.032.6576354
GCAGAGT82300.030.068929
ATGGGGG47900.026.952625
AGAGTAC74150.026.1936710-11
CAGAGTA78650.025.41352310-11
CGTATAC1451.342085E-424.6166273
ATGGGGA40300.023.1777535
GTCTTAG8200.021.7725891
Produced by FastQC (version 0.11.5)