FastQCFastQC Report
Mon 15 Aug 2016
SRR2921536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071377
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32410.1564659644284937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27920.13478956269187117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27950.080.6404341
GTATCAA58250.061.4183541
TCAACGC75850.046.4729164
CAACGCA77550.045.2732545
AACGCAG81150.043.329796
ATCAACG82200.043.028773
TATCAAC85000.041.893582
GTACATG104150.037.6139531
ACGCAGA96900.036.283527
TACATGG108550.035.602472
ACATGGG110900.034.4168243
CGCAGAG104400.033.7331128
CATGGGG89600.032.0311744
GCAGAGT109950.031.1646639
AGAGTAC100300.027.25400210-11
ATGGGGG46950.027.1237475
CAGAGTA106900.026.65652310-11
GTACCGT4800.026.0294916
GAGTACT58000.025.1807812-13
TACCGTA3202.0008883E-1124.1679087
Produced by FastQC (version 0.11.5)