##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921536_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2071377
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.389200517337017	33.0	31.0	34.0	28.0	34.0
2	31.44743038085293	33.0	31.0	34.0	28.0	34.0
3	31.530503138733316	33.0	31.0	34.0	28.0	34.0
4	35.056275125194496	37.0	35.0	37.0	32.0	37.0
5	34.73607894651722	37.0	35.0	37.0	32.0	37.0
6	34.720801669613984	37.0	35.0	37.0	32.0	37.0
7	34.574387955451854	37.0	35.0	37.0	31.0	37.0
8	34.53990026924119	37.0	35.0	37.0	31.0	37.0
9	36.139574302505046	39.0	37.0	39.0	31.0	39.0
10-11	36.012661625575646	39.0	37.0	39.0	30.0	39.0
12-13	35.92161470364883	39.0	37.0	39.0	30.0	39.0
14-15	37.12046334394946	40.0	37.0	41.0	31.0	41.0
16-17	37.104350149683036	40.0	37.0	41.0	31.0	41.0
18-19	37.115683673228006	40.0	37.0	41.0	30.5	41.0
20-21	37.09832275824246	40.0	37.0	41.0	30.0	41.0
22-23	37.0308263536768	40.0	37.0	41.0	30.0	41.0
24-25	36.965654248357495	40.0	37.0	41.0	30.0	41.0
26-27	36.83728794903101	40.0	37.0	41.0	30.0	41.0
28-29	36.652451002400824	40.0	36.0	41.0	30.0	41.0
30-31	36.605793151126036	40.0	36.0	41.0	30.0	41.0
32-33	36.48439999092391	40.0	36.0	41.0	30.0	41.0
34-35	36.35383249886428	40.0	35.5	41.0	30.0	41.0
36-37	36.22053638714729	40.0	35.0	41.0	29.0	41.0
38-39	36.04847089641335	40.0	35.0	41.0	28.5	41.0
40-41	35.84041026814529	40.0	35.0	41.0	27.5	41.0
42-43	35.66492820959198	39.0	35.0	41.0	27.0	41.0
44-45	35.44528639644063	39.0	35.0	41.0	26.0	41.0
46-47	35.16677215205151	39.0	35.0	41.0	25.5	41.0
48-49	34.91434562612214	39.0	34.5	41.0	24.0	41.0
50-51	34.690306255210906	39.0	34.0	41.0	24.0	41.0
52-53	34.48005457239314	38.0	34.0	41.0	23.0	41.0
54-55	34.25723443873326	38.0	34.0	40.0	23.0	41.0
56-57	33.99297544580248	37.5	33.5	40.0	22.5	41.0
58-59	33.83879709005169	37.0	33.5	40.0	22.5	41.0
60-61	33.71697836753039	37.0	33.0	40.0	22.0	41.0
62-63	33.44608127829941	36.0	33.0	40.0	22.0	41.0
64-65	33.14790813067829	36.0	33.0	39.0	22.0	41.0
66-67	32.84220207137571	35.0	33.0	39.0	21.0	41.0
68-69	32.525451426756206	35.0	33.0	38.5	20.0	40.5
70-71	32.229928689948764	35.0	32.5	37.5	20.0	40.0
72-73	31.919068088522756	35.0	32.0	37.0	20.0	39.0
74-75	31.629447222789477	35.0	32.0	36.5	19.0	39.0
76-77	31.347798831405388	35.0	32.0	36.0	18.0	38.5
78-79	31.077813454528076	35.0	32.0	35.5	18.0	37.0
80-81	30.861776972516353	35.0	32.0	35.0	17.0	37.0
82-83	30.68207284333079	35.0	31.5	35.0	15.5	36.5
84-85	30.495609201029072	35.0	31.0	35.0	14.0	36.0
86-87	30.3601174484413	35.0	31.0	35.0	12.0	36.0
88-89	30.258969999184117	35.0	31.0	35.0	10.5	35.5
90-91	30.168370122869955	35.0	31.0	35.0	10.0	35.0
92-93	30.241470046254257	35.0	31.5	35.0	10.0	35.0
94-95	30.216079207213365	35.0	32.0	35.0	9.0	35.0
96-97	30.200071981102425	35.0	32.0	35.0	7.5	35.0
98-99	30.162407422695146	35.0	32.0	35.0	7.0	35.0
100-101	30.114385020206363	35.0	32.0	35.0	7.0	35.0
102-103	29.21349252212417	33.5	29.5	35.0	5.5	35.0
104-105	29.93378704118082	34.5	31.0	35.0	2.0	35.0
106-107	30.04389205827814	35.0	32.0	35.0	2.0	35.0
108-109	30.0480504031859	35.0	32.0	35.0	2.0	35.0
110-111	30.018066967046558	35.0	32.0	35.0	2.0	35.0
112-113	29.964615325940184	35.0	32.0	35.0	2.0	35.0
114-115	29.90150465125373	35.0	32.0	35.0	2.0	35.0
116-117	29.824524700235642	35.0	32.0	35.0	2.0	35.0
118-119	29.72915649831006	35.0	32.0	35.0	2.0	35.0
120-121	29.60615860850053	35.0	31.0	35.0	2.0	35.0
122-123	29.483427449469602	35.0	31.0	35.0	2.0	35.0
124-125	28.554825364962532	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28410.0
3	10934.0
4	6391.0
5	5499.0
6	5984.0
7	6988.0
8	8159.0
9	9082.0
10	10601.0
11	11603.0
12	12213.0
13	14016.0
14	17622.0
15	14484.0
16	8141.0
17	6936.0
18	6176.0
19	5792.0
20	5713.0
21	5952.0
22	7073.0
23	9697.0
24	13454.0
25	18720.0
26	23259.0
27	25835.0
28	31007.0
29	36895.0
30	42926.0
31	52093.0
32	65346.0
33	84827.0
34	117887.0
35	189568.0
36	378168.0
37	593486.0
38	180164.0
39	276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.05889728126611	17.495037316192196	12.360574806144786	25.0854905963969
2	17.163413864055286	19.7878476852205	37.052596818873944	25.996141631850268
3	19.092379678498826	24.198782177883153	29.124877469325078	27.583960674292946
4	13.543908801082022	15.570959327601198	35.19544005410105	35.68969181721573
5	14.567247584632629	36.998111360665405	33.18842980235203	15.246211252349934
6	33.71190172673543	34.77585274462085	16.764640553173408	14.747604975470313
7	29.80733481599224	31.425013543828406	20.45736132423394	18.31029031594541
8	28.255706783735334	32.82287184196309	19.253276865371515	19.66814450893006
9	28.172686003021298	13.99948920893527	18.645273951538577	39.18255083650486
10-11	26.579979046267194	24.516374328297676	26.696455729204388	22.207190896230742
12-13	26.976395002601052	22.309878806678487	26.997710350254856	23.716015840465605
14-15	24.41298075808956	23.2692612218729	24.63018608495485	27.687571935082694
16-17	24.32291827606452	26.235124335640563	25.029379557814718	24.412577830480192
18-19	24.32048608093624	25.081375587806455	27.072047932176552	23.526090399080754
20-21	25.418964337635842	24.128647364057787	26.824508876602454	23.62787942170392
22-23	25.21027776798737	23.758575388339743	26.660290297022627	24.37085654665026
24-25	24.612173378983947	24.3414226208094	26.659884418517176	24.386519581689477
26-27	24.30913578482542	24.31521929108805	26.870798880248593	24.50484604383794
28-29	24.503188310068918	24.298247075259955	26.871974859511806	24.326589755159322
30-31	24.737835808463327	24.3172045477043	26.610540649513386	24.33441899431899
32-33	24.423090247474832	24.414471636925217	26.57052449677092	24.591913618829032
34-35	24.67221359220488	24.44999534027351	26.652476632558113	24.225314434963497
36-37	24.853528827805718	24.243139731615177	26.388929126011078	24.514402314568027
38-39	24.84526995036808	24.49040788374199	26.307717899867477	24.35660426602245
40-41	24.814169179133643	24.407297995361564	26.401338566120668	24.377194259384122
42-43	24.7716337478234	24.388566997693825	26.260157557565627	24.579641696917143
44-45	24.793218433761815	24.46094351302205	26.064013682107323	24.681824371108814
46-47	24.89296476613037	24.59630322247636	25.846516278964216	24.664215732429057
48-49	25.14011705755869	24.770600116758178	25.295529050236436	24.793753775446692
50-51	24.914295095265228	25.083073402026017	25.22150492984269	24.781126572866068
52-53	25.17862113872802	24.850628955492777	25.301126279525633	24.66962362625357
54-55	24.97200050506526	24.866834909087046	25.394618447581006	24.766546138266683
56-57	24.896514709578447	24.987584179504395	25.244857846400187	24.871043264516974
58-59	24.916973840120445	24.746600007532468	25.3838179382859	24.95260821406119
60-61	24.930038670368965	24.858839995161677	25.53980257034702	24.671318764122336
62-63	24.78886957126381	24.98117396703084	25.432660746797765	24.797295714907584
64-65	24.84793088221696	24.691172036133967	25.527669879824654	24.93322720182442
66-67	24.86263563825421	24.84873021253091	25.433240920191547	24.855393229023328
68-69	24.990668349019682	24.827768868646572	25.249129729988134	24.932433052345612
70-71	24.78321644556571	24.62061972647055	25.609623021860628	24.98654080610311
72-73	25.059693125428232	24.89409751478627	25.251869982216395	24.794339377569095
74-75	24.79881699661999	25.080492515692903	25.181023660067602	24.939666827619508
76-77	24.893613477066694	24.78323753496626	25.243330427475012	25.07981856049203
78-79	24.824811797333656	24.87503458853288	25.20314877584361	25.097004838289855
80-81	24.80641042077259	25.011172460048947	25.13256658025665	25.04985053892181
82-83	24.949865813952986	24.931132635490247	25.064292703016598	25.054708847540176
84-85	24.71614255157862	24.961542361342467	25.025952267141776	25.296362819937134
86-87	24.53284824840726	25.02371910782356	25.18853371251717	25.254898931252008
88-89	24.628257163544635	24.87509986337218	25.16648724054536	25.330155732537822
90-91	24.609697233352158	24.79751863306579	25.260891890919705	25.331892242662352
92-93	24.647550038238407	24.976849348402098	25.132072174027236	25.24352843933226
94-95	24.546735080369203	24.733195009227643	25.31562881341816	25.404441096984993
96-97	24.58799299385321	24.734590049649402	25.295728722305157	25.381688234192236
98-99	24.541100094890286	24.678330133712993	25.130042660799933	25.650527110596787
100-101	24.404138110512648	24.854639127223987	25.281360951959513	25.459861810303853
102-103	24.566795474164152	24.61239468007655	25.36539713070195	25.455412715057346
104-105	24.3732146072824	24.794153302020014	25.21294361140199	25.6196884792956
106-107	24.410643964794332	24.75039478683442	25.173834141735114	25.665127106636138
108-109	24.358645019452265	24.696096074925386	25.164090012057173	25.78116889356518
110-111	24.40517987806571	24.8179518640276	25.154566211643886	25.622302046262806
112-113	24.414577091968646	24.73620301323632	25.22447186286178	25.624748031933255
114-115	24.112997017856653	24.900921099448713	25.28029105950817	25.705790823186465
116-117	24.17164122896634	24.935182628075264	25.12072688156261	25.772449261395785
118-119	24.177917537549977	24.62283450006349	25.205789183230955	25.99345877915558
120-121	23.881932971499033	24.69316341733541	25.380486052707045	26.04441755845851
122-123	23.868994600179107	24.794905774694463	25.266478867799734	26.06962075732669
124-125	23.85836978989479	24.810421453866503	25.208037208026585	26.123171548212117
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	50.0
2	90.0
3	141.5
4	112.0
5	86.0
6	178.5
7	389.5
8	633.0
9	687.5
10	515.0
11	420.0
12	455.0
13	535.5
14	656.5
15	768.0
16	929.5
17	1076.5
18	1259.0
19	1492.5
20	1714.5
21	1974.0
22	2198.5
23	2512.0
24	2902.5
25	3487.5
26	4805.5
27	6195.0
28	7121.5
29	8680.5
30	11187.0
31	13478.0
32	16242.0
33	19115.5
34	21922.0
35	25033.0
36	28448.0
37	32454.0
38	35298.0
39	38376.0
40	41778.5
41	44606.0
42	47708.0
43	53014.0
44	58867.0
45	63188.0
46	68997.5
47	74452.5
48	80511.5
49	86211.0
50	86603.5
51	86354.5
52	86261.5
53	86598.0
54	88207.0
55	87820.0
56	83717.5
57	76918.0
58	72868.0
59	68447.0
60	60764.0
61	53785.0
62	46747.0
63	37055.0
64	28291.0
65	22661.5
66	17513.0
67	13442.5
68	11012.0
69	8963.5
70	7525.0
71	6189.0
72	5055.0
73	4728.0
74	3866.0
75	2846.5
76	1896.0
77	995.0
78	551.5
79	354.5
80	201.0
81	76.0
82	39.0
83	18.5
84	7.0
85	4.5
86	6.0
87	4.0
88	1.0
89	3.0
90	2.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11760292790737754
2	0.001689697240048528
3	0.022545388888647502
4	0.056822104329631924
5	0.027711034736795863
6	0.02013153568857818
7	0.015593491672447847
8	0.0055035852961580626
9	0.003862165120110921
10-11	0.007096728408203818
12-13	0.004803567868137959
14-15	0.0031380091600901236
16-17	0.010089906376289782
18-19	0.005648416488162222
20-21	0.01245548251235772
22-23	0.009076088032260664
24-25	0.01371068617639377
26-27	0.009800243992281464
28-29	0.014265872412409715
30-31	0.02133846228861284
32-33	0.01339688526038476
34-35	0.021241908160610067
36-37	0.015496937544445073
38-39	0.020831553116598282
40-41	0.00992093665228493
42-43	0.009534720140273836
44-45	0.016945249464486667
46-47	0.01593143112045755
48-49	0.020759137520596202
50-51	0.015303829288439527
52-53	0.02141087788461492
54-55	0.017259050380495678
56-57	0.020783276052596895
58-59	0.016559032952475575
60-61	0.019866011836570552
62-63	0.021314323756612148
64-65	0.017452158636501226
66-67	0.011731326552336923
68-69	0.02259366595264889
70-71	0.01457967332841873
72-73	0.01776595955251024
74-75	0.01820045312852272
76-77	0.013203777004379212
78-79	0.02915934665683746
80-81	0.021072938436605213
82-83	0.008955395372257199
84-85	0.0128899760883702
86-87	0.012648590768363268
88-89	0.020397059540585804
90-91	0.012576175172361188
92-93	0.007772607304223229
94-95	0.007458806388214217
96-97	0.0025586843920734855
98-99	0.0018103899000519944
100-101	0.011514079764330684
102-103	0.00333111741609567
104-105	0.002606961456074872
106-107	2.896623840083191E-4
108-109	0.01979359624056847
110-111	7.482944920214911E-4
112-113	0.008786425648252346
114-115	0.002317299072066553
116-117	0.008351932072239866
118-119	0.00984852105628285
120-121	0.0038138880561095346
122-123	5.793247680166382E-4
124-125	0.02575581364473971
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2071377.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.18411833603783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.78909937652166	42.14933765871256
2	11.47381350966459	12.433969379465912
3	3.9608549008846285	6.438462919842243
4	1.9271339045537215	4.1768020613491785
5	1.0979568712782934	2.9745912520604443
6	0.7212454935788299	2.3448030704045597
7	0.5080000788690076	1.9267875471708396
8	0.3844670095474733	1.6665604753298302
9	0.2876217831478695	1.4026079460695735
>10	1.717906224573545	16.728053976661677
>50	0.09463786687644178	3.475169327971514
>100	0.03520878029764362	3.312855270859965
>500	0.0016969479964795856	0.5382323960805979
>1k	3.5725220978516804E-4	0.43176671802112937
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3241	0.1564659644284937	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2792	0.13478956269187117	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.655412800277304E-5
2	0.0	0.0	0.0	0.0	9.655412800277304E-5
3	0.0	0.0	0.0	0.0	9.655412800277304E-5
4	0.0	0.0	0.0	9.655412800277304E-5	9.655412800277304E-5
5	0.0	0.0	0.0	9.655412800277304E-5	9.655412800277304E-5
6	0.0	0.0	0.0	9.655412800277304E-5	1.4483119200415956E-4
7	0.0	0.0	0.0	9.655412800277304E-5	1.4483119200415956E-4
8	0.0	0.0	0.0	9.655412800277304E-5	1.4483119200415956E-4
9	0.0	0.0	0.0	9.655412800277304E-5	1.9310825600554608E-4
10-11	0.0	0.0	0.0	9.655412800277304E-5	2.6552385200762584E-4
12-13	0.0	0.0	0.0	9.655412800277304E-5	2.896623840083191E-4
14-15	0.0	0.0	0.0	9.655412800277304E-5	3.8621651201109215E-4
16-17	0.0	0.0	0.0	9.655412800277304E-5	4.3449357601247864E-4
18-19	0.0	0.0	0.0	9.655412800277304E-5	4.3449357601247864E-4
20-21	0.0	0.0	0.0	9.655412800277304E-5	4.586321080131719E-4
22-23	0.0	0.0	0.0	1.9310825600554608E-4	5.069091720145585E-4
24-25	0.0	0.0	0.0	3.620779800103989E-4	5.310477040152517E-4
26-27	0.0	0.0	0.0	4.827706400138652E-4	5.793247680166382E-4
28-29	0.0	0.0	0.0	0.0012793421960367425	6.276018320180248E-4
30-31	0.0	0.0	0.0	0.004707013740135185	7.000174280201044E-4
32-33	0.0	0.0	0.0	0.012793421960367427	7.241559600207978E-4
34-35	0.0	0.0	0.0	0.02331782191266969	8.207100880235707E-4
36-37	0.0	0.0	0.0	0.03958719248113694	8.44848620024264E-4
38-39	0.0	0.0	0.0	0.06314639971381357	8.689871520249573E-4
40-41	0.0	0.0	0.0	0.09020569408659071	8.689871520249573E-4
42-43	0.0	0.0	0.0	0.11625117011533873	8.689871520249573E-4
44-45	0.0	0.0	0.0	0.1475347075882372	9.172642160263438E-4
46-47	0.0	0.0	0.0	0.18562531108533115	9.655412800277304E-4
48-49	0.0	0.0	0.0	0.22567113567448127	9.655412800277304E-4
50-51	0.0	0.0	0.0	0.2690722162117278	0.001013818344029117
52-53	0.0	0.0	0.0	0.31039738299691466	0.001013818344029117
54-55	0.0	0.0	0.0	0.35604334701022555	0.001013818344029117
56-57	0.0	0.0	0.0	0.4087136238357383	0.0010862339400311968
58-59	0.0	0.0	0.0	0.4662116070613896	0.0011586495360332765
60-61	0.0	0.0	0.0	0.5290924829231956	0.0011586495360332765
62-63	0.0	0.0	0.0	0.5964631257371305	0.0011586495360332765
64-65	0.0	0.0	0.0	0.6643406777230798	0.0011586495360332765
66-67	0.0	0.0	0.0	0.7410046553572817	0.0011586495360332765
68-69	0.0	0.0	0.0	0.8326345228319133	0.0011586495360332765
70-71	0.0	0.0	0.0	0.9303473003707196	0.0012069266000346629
72-73	0.0	0.0	0.0	1.034505065953711	0.0013517577920388224
74-75	0.0	0.0	0.0	1.1435870920648439	0.001400034856040209
76-77	0.0	0.0	0.0	1.2671763759083934	0.0014241733880409023
78-79	0.0	0.0	0.0	1.4026900945602852	0.001496588984042982
80-81	0.0	0.0	0.0	1.5547145691006514	0.0015448660480443686
82-83	0.0	0.0	0.0	1.7172151665293183	0.001593143112045755
84-85	0.0	0.0	0.0	1.8824916951380652	0.0016172816440464483
86-87	0.0	0.0	0.0	2.0611168319431954	0.0016414201760471415
88-89	0.0	0.0	0.0	2.2632770374490017	0.0016414201760471415
90-91	0.0	0.0	0.0	2.4896481905515024	0.0016414201760471415
92-93	0.0	0.0	0.0	2.7308404023024297	0.0018103899000519944
94-95	0.0	0.0	0.0	2.978308632373537	0.0019310825600554608
96-97	0.0	0.0	0.0	3.2458359825372205	0.0019793596240568474
98-99	0.0	0.0	0.0	3.539022592217641	0.0020517752200589273
100-101	0.0	0.0	0.0	3.861513379746903	0.0020759137520596203
102-103	0.0	0.0	0.0	4.208770301108876	0.0020759137520596203
104-105	0.0	0.0	0.0	4.567227501319171	0.0020759137520596203
106-107	0.0	0.0	0.0	4.946274869326057	0.0021000522840603133
108-109	0.0	0.0	0.0	5.33982949506536	0.0021966064120630866
110-111	0.0	0.0	0.0	5.77330925273381	0.002317299072066553
112-113	0.0	0.0	0.0	6.235248339631076	0.0023655761360679393
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2795	0.0	80.640434	1
GTATCAA	5825	0.0	61.418354	1
TCAACGC	7585	0.0	46.472916	4
CAACGCA	7755	0.0	45.273254	5
AACGCAG	8115	0.0	43.32979	6
ATCAACG	8220	0.0	43.02877	3
TATCAAC	8500	0.0	41.89358	2
GTACATG	10415	0.0	37.613953	1
ACGCAGA	9690	0.0	36.28352	7
TACATGG	10855	0.0	35.60247	2
ACATGGG	11090	0.0	34.416824	3
CGCAGAG	10440	0.0	33.733112	8
CATGGGG	8960	0.0	32.031174	4
GCAGAGT	10995	0.0	31.164663	9
AGAGTAC	10030	0.0	27.254002	10-11
ATGGGGG	4695	0.0	27.123747	5
CAGAGTA	10690	0.0	26.656523	10-11
GTACCGT	480	0.0	26.029491	6
GAGTACT	5800	0.0	25.18078	12-13
TACCGTA	320	2.0008883E-11	24.167908	7
>>END_MODULE