FastQCFastQC Report
Mon 15 Aug 2016
SRR2921535_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921535_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773071
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20350.066.962861
GTATCAA38650.059.5832751
CAACGCA50300.045.0644535
TCAACGC50450.044.9304664
ATCAACG54050.042.0455743
AACGCAG53900.041.945396
GTACATG98150.039.3475151
TATCAAC57700.039.076532
TACATGG104100.036.0604552
ACGCAGA64450.034.8013087
ACATGGG107450.034.4378933
CGCAGAG68400.032.8776328
CATGGGG92450.030.889544
GCAGAGT74000.029.745599
GTATACG1055.903271E-428.336331
ATGGGGG48300.028.084385
AGAGTAC66250.025.72712910-11
CAGAGTA70350.025.4962210-11
GTATAGA5600.024.4400841
CATGGGT18300.022.4323674
Produced by FastQC (version 0.11.5)