FastQCFastQC Report
Mon 15 Aug 2016
SRR2921535_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921535_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773071
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29590.16688559002995368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.14567944543675918No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.082.802391
GTATCAA54850.062.913621
TCAACGC74500.045.8664934
CAACGCA75300.045.2796945
ATCAACG78650.043.5232433
AACGCAG78850.043.384696
TATCAAC82900.041.722832
ACGCAGA92600.036.8130467
CGCAGAG97650.034.969188
GTACATG93700.034.6656761
GCAGAGT102450.032.692069
TACATGG98000.032.681982
ACATGGG101950.031.182163
GTATAGG4200.028.3807261
CATGGGG87150.027.9380344
AGAGTAC95850.027.89866310-11
CAGAGTA98650.027.83046310-11
GAGTACT53350.025.42485412-13
AGTACTT54550.024.75649812-13
ATGGGGG45200.024.7493175
Produced by FastQC (version 0.11.5)