FastQCFastQC Report
Mon 15 Aug 2016
SRR2921534_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921534_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959343
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23700.072.05971
GTATCAA47200.058.2450331
TCAACGC58050.046.527824
CAACGCA59850.044.830285
AACGCAG62800.042.726586
ATCAACG66750.040.6407363
TATCAAC69400.039.603222
GTACATG106850.037.647061
ACGCAGA73350.036.172857
TACATGG113150.035.016092
CGCAGAG76850.034.3688438
ACATGGG114700.034.1798363
GCAGAGT82600.030.9688669
CATGGGG92450.030.5021534
ATGGGGG50700.028.0445845
CAGAGTA79150.026.64485210-11
AGAGTAC75100.026.61627810-11
GTATCGC1651.1110591E-525.2403496
GTGGTAT10250.023.8022981
CATGGGT19650.023.3124144
Produced by FastQC (version 0.11.5)