##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921534_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1959343
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.147730642363282	31.0	31.0	34.0	26.0	34.0
2	30.24067404226825	31.0	31.0	34.0	26.0	34.0
3	30.31211278474468	31.0	31.0	34.0	26.0	34.0
4	33.70190466906509	37.0	35.0	37.0	28.0	37.0
5	33.74368908353463	37.0	35.0	37.0	28.0	37.0
6	33.70086554523634	37.0	35.0	37.0	28.0	37.0
7	33.60676308333967	37.0	35.0	37.0	28.0	37.0
8	33.55592767575662	37.0	35.0	37.0	27.0	37.0
9	35.03749828386352	39.0	35.0	39.0	27.0	39.0
10-11	34.93817391850227	39.0	35.0	39.0	27.0	39.0
12-13	34.80712488829164	39.0	35.0	39.0	27.0	39.0
14-15	35.98668048422354	40.0	36.0	41.0	27.0	41.0
16-17	35.91528869626196	40.0	36.0	41.0	27.0	41.0
18-19	35.92733737788636	40.0	36.0	41.0	27.0	41.0
20-21	35.96173742933218	40.0	36.0	41.0	27.0	41.0
22-23	35.92573735175515	40.0	36.0	41.0	27.0	41.0
24-25	35.8854062305579	40.0	36.0	41.0	27.0	41.0
26-27	35.81021418914401	40.0	36.0	41.0	27.0	41.0
28-29	35.76661258391206	40.0	36.0	41.0	27.0	41.0
30-31	35.67211407089009	40.0	35.0	41.0	26.0	41.0
32-33	35.5690945893598	40.0	35.0	41.0	26.0	41.0
34-35	35.40209881577651	40.0	35.0	41.0	25.0	41.0
36-37	35.266698582126764	40.0	35.0	41.0	25.0	41.0
38-39	35.13913975245784	39.5	35.0	41.0	24.5	41.0
40-41	34.99070734424754	39.0	35.0	41.0	24.0	41.0
42-43	34.789516945220925	39.0	34.5	41.0	23.5	41.0
44-45	34.5966757734608	39.0	34.0	41.0	23.0	41.0
46-47	34.34536091945106	39.0	34.0	41.0	22.0	41.0
48-49	34.20065093248094	39.0	33.5	41.0	20.5	41.0
50-51	33.99525325581075	38.0	33.0	40.5	20.0	41.0
52-53	33.8034310480605	38.0	33.0	40.0	19.0	41.0
54-55	33.59393301734306	38.0	33.0	40.0	18.0	41.0
56-57	33.345214952154876	37.0	33.0	40.0	17.5	41.0
58-59	33.09115504533918	37.0	33.0	40.0	16.0	41.0
60-61	32.775698282536545	36.0	32.0	40.0	13.5	41.0
62-63	32.44691332757971	36.0	32.0	39.0	12.5	41.0
64-65	32.13495110350766	35.0	32.0	39.0	9.5	41.0
66-67	32.08765795473279	35.0	32.0	39.0	9.5	41.0
68-69	31.83709820077444	35.0	32.0	38.5	8.0	40.0
70-71	31.538232458533294	35.0	32.0	37.0	7.0	40.0
72-73	31.21275167237181	35.0	31.0	37.0	6.0	39.0
74-75	30.913382700221455	35.0	31.0	36.0	2.0	39.0
76-77	30.641970293103352	35.0	31.0	36.0	2.0	38.5
78-79	30.37548402704376	35.0	31.0	35.5	2.0	37.0
80-81	30.142351032973806	35.0	31.0	35.0	2.0	37.0
82-83	29.950730423412338	35.0	31.0	35.0	2.0	36.5
84-85	29.757797384123148	35.0	30.5	35.0	2.0	36.0
86-87	29.609603321113248	35.0	30.0	35.0	2.0	36.0
88-89	29.490217128904945	35.0	30.0	35.0	2.0	35.5
90-91	29.37917710171216	34.5	30.0	35.0	2.0	35.0
92-93	29.28531834395509	34.0	30.0	35.0	2.0	35.0
94-95	29.220111792575366	34.0	30.0	35.0	2.0	35.0
96-97	29.122764110214497	34.0	30.0	35.0	2.0	35.0
98-99	29.056096609935064	34.0	30.0	35.0	2.0	35.0
100-101	28.99001399959068	34.0	30.0	35.0	2.0	35.0
102-103	27.997282507452752	33.5	28.0	34.5	2.0	35.0
104-105	28.83685755888581	34.0	29.5	35.0	2.0	35.0
106-107	29.167423978343763	35.0	30.0	35.0	2.0	35.0
108-109	29.201614520785796	35.0	31.0	35.0	2.0	35.0
110-111	29.14262995299955	35.0	31.0	35.0	2.0	35.0
112-113	29.072839977482246	35.0	30.0	35.0	2.0	35.0
114-115	28.95220719394205	35.0	30.0	35.0	2.0	35.0
116-117	28.88589389402468	35.0	30.0	35.0	2.0	35.0
118-119	28.721939446028593	35.0	30.0	35.0	2.0	35.0
120-121	28.54430413664172	35.0	30.0	35.0	2.0	35.0
122-123	28.422613090204216	35.0	30.0	35.0	2.0	35.0
124-125	27.559996896919017	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	63955.0
3	11616.0
4	7685.0
5	6795.0
6	7303.0
7	8577.0
8	9410.0
9	10100.0
10	10643.0
11	11139.0
12	11194.0
13	11867.0
14	13007.0
15	11271.0
16	8038.0
17	7246.0
18	6708.0
19	6358.0
20	6152.0
21	6428.0
22	7781.0
23	10324.0
24	14782.0
25	19942.0
26	23818.0
27	25767.0
28	30557.0
29	36010.0
30	41466.0
31	49956.0
32	63412.0
33	83022.0
34	116445.0
35	185104.0
36	355103.0
37	521018.0
38	139243.0
39	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.90520828546776	17.046732121249253	12.066516560213621	24.98154303306937
2	16.934520907537575	19.375354482936068	37.97621958721467	25.713905022311685
3	19.12959666414647	24.197190274443177	28.800580343764853	27.8726327176455
4	13.451276741735487	15.091365043579124	35.54700582416416	35.91035239052123
5	14.478641869165749	37.19068643768392	33.31386455859318	15.016807134557142
6	33.57760914032685	34.48036755747681	17.338629379884622	14.603393922311717
7	30.045089180179552	31.19720938259326	20.440598540868997	18.317102896358197
8	28.311944648097587	32.79243853475934	19.47895202249692	19.416664794646156
9	28.153688283974127	14.370140445279436	18.70403365377252	38.772137616973914
10-11	26.51853126359364	24.635715875742097	26.85368797469259	21.992064885971665
12-13	26.836594836752088	22.44314461398175	26.814921540881986	23.90533900838417
14-15	24.37803172677661	23.50876440588749	24.228797122115566	27.884406745220332
16-17	24.475784042350323	26.429102598238398	24.684043396981185	24.411069962430094
18-19	24.51046824598859	25.689598034264545	26.183814852393674	23.616118867353197
20-21	25.6981131882762	24.60147760333495	25.862373612927573	23.838035595461275
22-23	25.52363259597271	24.197118814313093	25.65080981325043	24.628438776463764
24-25	24.915063328731026	24.71650111729547	25.72164570689654	24.646789847076967
26-27	24.510958077671237	24.783936646096254	25.905875498590554	24.79922977764195
28-29	24.542903288563632	24.845600448939514	25.94494567739979	24.666550585097067
30-31	25.015108007895225	24.734776910827904	25.652312335819293	24.597802745457575
32-33	24.687772083962077	24.89040043099376	25.59278238644923	24.82904509859493
34-35	24.954169660966542	24.90030417406466	25.77938381456998	24.366142350398825
36-37	24.976951324721174	24.705626736310133	25.59134437031479	24.726077568653903
38-39	24.980389873821093	24.996986985821472	25.47937566260778	24.543247477749656
40-41	25.047762240422465	24.768631103424557	25.657367486750015	24.52623916940296
42-43	24.773181938304056	24.780637641342384	25.597011590809572	24.849168829543988
44-45	24.781504655369858	24.80872321710122	25.529938502902503	24.879833624626414
46-47	24.908636575807126	24.890864372554407	25.393924970660542	24.80657408097792
48-49	24.980181571273963	25.052281078806754	25.1526433890445	24.814893960874784
50-51	24.840651634921247	25.2546956864305	25.061498958443174	24.84315372020508
52-53	25.002451248247105	25.041390348005756	25.236392252829916	24.719766150917227
54-55	24.865364436399474	24.96008292335462	25.385090646357934	24.789461993887976
56-57	24.796463195388696	25.116301274808002	25.097051760768878	24.990183769034424
58-59	24.928604946006296	24.983418170009326	25.213342667154805	24.874634216829573
60-61	24.972386014291626	25.09210830962075	25.34508228546543	24.590423390622195
62-63	24.818575573810755	25.15265283281483	25.289719988828352	24.739051604546063
64-65	24.84098466330106	24.886835202287422	25.34814881001768	24.924031324393837
66-67	24.757576732267605	25.13439027989019	25.267695634926106	24.8403373529161
68-69	24.79944536211896	25.02811096127107	25.214275084358412	24.95816859225156
70-71	24.664193365797217	24.86624797058183	25.460718455520542	25.008840208100413
72-73	24.92917557768682	25.162542623564306	25.19559997692367	24.712681821825207
74-75	24.619277158876063	25.34837792948656	25.205161706688067	24.827183204949307
76-77	24.693141125981384	24.861873939995448	25.30787995004896	25.13710498397421
78-79	24.720548709619035	24.953406703257176	25.2916915236576	25.034353063466185
80-81	24.69701042114154	25.22147510190267	25.09384949213941	24.987664984816384
82-83	24.821075484310462	25.107574209550656	25.19560397376922	24.875746332369662
84-85	24.631670563252307	25.178843071394297	25.044538484313268	25.14494788104013
86-87	24.368000093925495	25.330455673929475	25.250006444616247	25.051537787528787
88-89	24.584095349795867	25.032818288905595	25.281632350932988	25.101454010365554
90-91	24.585722288650032	24.93271791547405	25.35048574538596	25.131074050489953
92-93	24.683991452633684	25.164831855172576	25.185863358526895	24.965313333666842
94-95	24.569968382505326	24.951864227515287	25.307982166793767	25.17018522318562
96-97	24.547566801369385	24.965405545015155	25.245683158178966	25.24134449543649
98-99	24.42085055404893	24.95915264125836	25.207120737186994	25.412876067505714
100-101	24.383693518053217	25.092127268982527	25.335400938334	25.18877827463025
102-103	24.4247767284643	24.869112285899856	25.41986788092695	25.286243104708895
104-105	24.28193216061935	25.057216764256722	25.348685392524356	25.31216568259957
106-107	24.359628421531625	25.001256919116425	25.32956164020671	25.309553019145238
108-109	24.33895342768245	24.926961573378	25.3628823654471	25.371202633492445
110-111	24.32982844945574	25.141095799166862	25.342808880173394	25.186266871204005
112-113	24.247538455374208	25.160468388282588	25.46679450884334	25.125198647499868
114-115	24.07957525021986	25.349828014518337	25.411894892600312	25.158701842661486
116-117	24.06002354024453	25.36512389253154	25.36458795895055	25.210264608273388
118-119	24.25978906955875	24.947789241462864	25.361075383023856	25.431346305954534
120-121	23.933662094656114	25.081629020464558	25.60097117341157	25.38373771146776
122-123	23.93011131881229	25.22231726614536	25.476842680014002	25.370728735028347
124-125	23.82989213609425	25.269506646465313	25.457943743972578	25.442657473467854
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	129.0
1	139.5
2	163.0
3	173.5
4	161.0
5	154.0
6	156.0
7	200.5
8	265.0
9	297.5
10	326.0
11	369.0
12	420.0
13	497.0
14	587.5
15	673.0
16	781.5
17	878.0
18	984.5
19	1068.5
20	1152.5
21	1345.0
22	1565.0
23	1826.5
24	2184.5
25	2860.0
26	4092.5
27	5467.0
28	6427.0
29	7971.5
30	10319.0
31	12492.5
32	15684.5
33	18946.0
34	22085.0
35	25286.0
36	28785.0
37	32783.5
38	34872.5
39	37524.5
40	40538.0
41	42663.0
42	45317.5
43	50258.5
44	55560.0
45	58954.0
46	63475.0
47	68453.0
48	74101.5
49	79636.5
50	80655.0
51	80771.5
52	81407.5
53	82034.5
54	83585.5
55	83601.0
56	80551.5
57	74574.0
58	70401.0
59	66100.0
60	59341.0
61	52307.5
62	44756.5
63	35380.5
64	26387.5
65	20429.5
66	15698.0
67	11907.0
68	9449.5
69	7691.0
70	6523.0
71	5379.5
72	4402.5
73	4073.5
74	3384.0
75	2648.0
76	1890.5
77	1096.5
78	669.0
79	414.0
80	250.5
81	144.5
82	91.5
83	72.0
84	45.5
85	28.5
86	22.0
87	19.5
88	17.5
89	14.5
90	13.5
91	11.5
92	9.0
93	6.5
94	7.5
95	10.5
96	11.5
97	13.0
98	10.0
99	6.0
100	22.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03741049933574673
2	0.02572290813808506
3	0.02572290813808506
4	0.03097977230122546
5	0.033225423011693206
6	0.04598480204844175
7	0.039809262594655455
8	0.034450323399221064
9	0.02924449675222766
10-11	0.03608352391592488
12-13	0.03631319273858635
14-15	0.03480758601225002
16-17	0.037129792996938256
18-19	0.050629216017818224
20-21	0.05981596892427717
22-23	0.05369146698663787
24-25	0.06463901420016811
26-27	0.04835804654927698
28-29	0.04848564033964446
30-31	0.04820493400083599
32-33	0.055018442406459714
34-35	0.038814031029789066
36-37	0.05039954719515675
38-39	0.059764931408130176
40-41	0.04820493400083599
42-43	0.05667716168123703
44-45	0.05718753684270697
46-47	0.06272510734465583
48-49	0.04789870890395403
50-51	0.0498381345175398
52-53	0.05905040618207225
54-55	0.04634206466147071
56-57	0.043483963757239036
58-59	0.04460678911247291
60-61	0.032842641640590746
62-63	0.041544538143653255
64-65	0.041110719256403805
66-67	0.03480758601225002
68-69	0.029984540736359075
70-71	0.049072571775334896
72-73	0.03194948510801835
74-75	0.03909473736859753
76-77	0.03189844759187136
78-79	0.032587454059855775
80-81	0.024931826637806652
82-83	0.01768449934493348
84-85	0.018526618361358882
86-87	0.017531386796492496
88-89	0.022992401024220874
90-91	0.0122745226333521
92-93	0.0192411435874168
94-95	0.015362292360245245
96-97	0.010922028455456752
98-99	0.011228253552338717
100-101	0.011866222504176146
102-103	0.006405208276447769
104-105	0.006481764550668259
106-107	0.009569534277561407
108-109	0.013856685633908917
110-111	0.006354170760300775
112-113	0.007987371277004588
114-115	0.008472227680401033
116-117	0.0074004398413141555
118-119	0.2431937644404272
120-121	0.36060557033658736
122-123	0.030596990930123007
124-125	0.0034960698560691007
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1959343.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.006031890695645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.29489092187819	41.50101485083612
2	11.034029647181123	11.697402547227277
3	3.7361170909465216	5.9411022501025315
4	1.8675705786909378	3.9597002260886707
5	1.090574616944723	2.8903516462477588
6	0.7185784945044212	2.2853396757401647
7	0.5345705697968837	1.9834825269336671
8	0.3872562696563741	1.642153454342208
9	0.31900902087556887	1.5218462100554995
>10	1.85689656158125	18.153051758245056
>50	0.11621612099734832	4.1965419414553375
>100	0.04341624817175975	3.8329051373801324
>500	5.825725165929745E-4	0.1914036494086655
>1k	2.912862582964868E-4	0.20370412593694465
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0207503229398834E-4
2	0.0	0.0	0.0	0.0	1.0207503229398834E-4
3	0.0	0.0	0.0	0.0	1.0207503229398834E-4
4	0.0	0.0	0.0	0.0	1.0207503229398834E-4
5	0.0	0.0	0.0	0.0	1.0207503229398834E-4
6	0.0	0.0	0.0	0.0	1.5311254844098252E-4
7	0.0	0.0	0.0	0.0	1.5311254844098252E-4
8	0.0	0.0	0.0	0.0	1.5311254844098252E-4
9	0.0	0.0	0.0	0.0	1.5311254844098252E-4
10-11	0.0	0.0	0.0	5.103751614699417E-5	1.5311254844098252E-4
12-13	0.0	0.0	0.0	5.103751614699417E-5	2.0415006458797668E-4
14-15	0.0	0.0	0.0	5.103751614699417E-5	2.8070633880846797E-4
16-17	0.0	0.0	0.0	5.103751614699417E-5	3.572626130289592E-4
18-19	0.0	0.0	0.0	5.103751614699417E-5	3.572626130289592E-4
20-21	0.0	0.0	0.0	5.103751614699417E-5	4.0830012917595336E-4
22-23	0.0	0.0	0.0	5.103751614699417E-5	4.593376453229475E-4
24-25	0.0	0.0	0.0	1.0207503229398834E-4	4.593376453229475E-4
26-27	0.0	0.0	0.0	3.0622509688196505E-4	4.593376453229475E-4
28-29	0.0	0.0	0.0	0.0013014566617483513	4.593376453229475E-4
30-31	0.0	0.0	0.0	0.005027195340478926	4.848564033964446E-4
32-33	0.0	0.0	0.0	0.012070372568764122	5.86931435690433E-4
34-35	0.0	0.0	0.0	0.0210019378944881	6.634877099109242E-4
36-37	0.0	0.0	0.0	0.03715531175501176	6.634877099109242E-4
38-39	0.0	0.0	0.0	0.06203610087667141	6.634877099109242E-4
40-41	0.0	0.0	0.0	0.08962187835412176	6.634877099109242E-4
42-43	0.0	0.0	0.0	0.11611034923441174	6.890064679844213E-4
44-45	0.0	0.0	0.0	0.1472432340840782	7.145252260579184E-4
46-47	0.0	0.0	0.0	0.18610830263001427	7.400439841314155E-4
48-49	0.0	0.0	0.0	0.22303394556236453	9.18675290645895E-4
50-51	0.0	0.0	0.0	0.26371084593151883	9.18675290645895E-4
52-53	0.0	0.0	0.0	0.3040304836876443	9.697128067928893E-4
54-55	0.0	0.0	0.0	0.35152089246242235	0.0010207503229398833
56-57	0.0	0.0	0.0	0.402737039915931	0.0010207503229398833
58-59	0.0	0.0	0.0	0.45836793251615465	0.0010207503229398833
60-61	0.0	0.0	0.0	0.5218330838449419	0.0010207503229398833
62-63	0.0	0.0	0.0	0.5891515676428272	0.0010207503229398833
64-65	0.0	0.0	0.0	0.6588177771834742	0.0010207503229398833
66-67	0.0	0.0	0.0	0.7377983334209477	0.0010207503229398833
68-69	0.0	0.0	0.0	0.827956105694613	0.0010207503229398833
70-71	0.0	0.0	0.0	0.924212861147844	0.0010717878390868775
72-73	0.0	0.0	0.0	1.0238891301829236	0.0010973065971603747
74-75	0.0	0.0	0.0	1.1275463254774687	0.0011228253552338719
76-77	0.0	0.0	0.0	1.2490156139073147	0.0011228253552338719
78-79	0.0	0.0	0.0	1.3813048557603236	0.0011228253552338719
80-81	0.0	0.0	0.0	1.5295433214092684	0.001173862871380866
82-83	0.0	0.0	0.0	1.6877596214649504	0.0012759379036748543
84-85	0.0	0.0	0.0	1.8460779965529261	0.0013014566617483513
86-87	0.0	0.0	0.0	2.018814469952428	0.001454569210189334
88-89	0.0	0.0	0.0	2.2121700998753155	0.0015821630005568193
90-91	0.0	0.0	0.0	2.429105062258114	0.0015821630005568193
92-93	0.0	0.0	0.0	2.6639541928085078	0.0015821630005568193
94-95	0.0	0.0	0.0	2.9107205833792245	0.0015821630005568193
96-97	0.0	0.0	0.0	3.161034081322158	0.0016332005167038134
98-99	0.0	0.0	0.0	3.435105543031516	0.0016332005167038134
100-101	0.0	0.0	0.0	3.750185648964985	0.0016332005167038134
102-103	0.0	0.0	0.0	4.087518111938543	0.0016842380328508076
104-105	0.0	0.0	0.0	4.441054986288771	0.0016842380328508076
106-107	0.0	0.0	0.0	4.807223645885381	0.0017097567909243046
108-109	0.0	0.0	0.0	5.18834629771306	0.0017352755489978017
110-111	0.0	0.0	0.0	5.6015715471971985	0.00183735058129179
112-113	0.0	0.0	0.0	6.050880320597262	0.0018883880974387844
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2370	0.0	72.0597	1
GTATCAA	4720	0.0	58.245033	1
TCAACGC	5805	0.0	46.52782	4
CAACGCA	5985	0.0	44.83028	5
AACGCAG	6280	0.0	42.72658	6
ATCAACG	6675	0.0	40.640736	3
TATCAAC	6940	0.0	39.60322	2
GTACATG	10685	0.0	37.64706	1
ACGCAGA	7335	0.0	36.17285	7
TACATGG	11315	0.0	35.01609	2
CGCAGAG	7685	0.0	34.368843	8
ACATGGG	11470	0.0	34.179836	3
GCAGAGT	8260	0.0	30.968866	9
CATGGGG	9245	0.0	30.502153	4
ATGGGGG	5070	0.0	28.044584	5
CAGAGTA	7915	0.0	26.644852	10-11
AGAGTAC	7510	0.0	26.616278	10-11
GTATCGC	165	1.1110591E-5	25.240349	6
GTGGTAT	1025	0.0	23.802298	1
CATGGGT	1965	0.0	23.312414	4
>>END_MODULE