FastQCFastQC Report
Mon 15 Aug 2016
SRR2921534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959343
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28120.1435174954053476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24570.12539917717316468No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27600.076.88151
GTATCAA56250.061.565091
TCAACGC74300.045.762154
CAACGCA76050.044.6771475
AACGCAG78150.043.6210946
TATCAAC81650.041.8615042
ATCAACG82400.041.335953
GTACATG106450.035.8916361
GTATAGG6350.035.6689871
ACGCAGA95350.035.6257677
CGCAGAG100450.033.7560428
TACATGG113450.033.3294642
ACATGGG114500.033.179843
CATGGGG90950.031.3611454
GCAGAGT107950.030.7495069
AGAGTAC99650.027.28167710-11
CAGAGTA104200.026.26166510-11
ATGGGGG49850.026.2606875
GTGGTAT10850.025.8195721
GAGTACT52650.025.70649512-13
Produced by FastQC (version 0.11.5)