FastQCFastQC Report
Mon 15 Aug 2016
SRR2921533_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921533_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910203
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20120.10532911947054842No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.076.482091
GTATCAA44450.064.808241
TCAACGC56650.050.2011874
CAACGCA59150.048.2793125
AACGCAG61900.046.12966
ATCAACG61450.045.8937763
TATCAAC65900.043.5170062
ACGCAGA72100.039.521137
GTACATG103850.037.7116971
CGCAGAG77450.036.7133678
TACATGG108900.035.6765372
ACATGGG110350.035.099913
GCAGAGT81550.033.8463449
CATGGGG89750.032.018334
ACTATCG1852.878005E-828.9393358
AGAGTAC75450.027.12099310-11
ATGGGGG46350.027.083665
CAGAGTA79700.026.90625810-11
CCTATAC3700.025.7285693
ATGGGGA35950.024.8237255
Produced by FastQC (version 0.11.5)