FastQCFastQC Report
Mon 15 Aug 2016
SRR2921533_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921533_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910203
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32950.1724947557929707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29150.15260158213551125No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.079.496791
GTATCAA60250.062.2451
TCAACGC82850.044.777494
CAACGCA84900.043.6688235
ATCAACG86900.042.827673
AACGCAG88600.041.9696246
TATCAAC89250.041.835632
GTACATG102950.036.2542421
ACGCAGA102500.036.1601527
CGCAGAG107150.034.645528
TACATGG107950.034.257522
ACATGGG108150.033.9170763
GCAGAGT112000.032.5078359
TAGATCG953.283833E-431.321145
CATGGGG87350.030.6770674
GTGGTAT10450.029.0981081
AGAGTAC107500.028.38988110-11
CAGAGTA111200.026.32176210-11
GAGTACT60550.025.2487312-13
TATAGGG3351.8189894E-1224.8868662
Produced by FastQC (version 0.11.5)