FastQCFastQC Report
Mon 15 Aug 2016
SRR2921532_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921532_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201810
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24850.070.166751
GTATCAA47000.055.205041
TCAACGC57800.043.5415574
CAACGCA59400.042.367765
ATCAACG63350.040.0086983
AACGCAG63950.039.6342246
TATCAAC66600.038.502992
GTACATG109450.038.3865431
TACATGG113800.036.597142
ACATGGG115500.035.2858053
ACGCAGA76400.032.8625037
CATGGGG94100.031.7398974
CGCAGAG80800.030.7721398
GCAGAGT90000.026.8995299
ATGGGGG50550.026.2461285
CAGAGTA84600.024.50277510-11
AGAGTAC80400.023.71127310-11
CATGGGT24350.022.4791724
ATGGGGA38050.022.2031845
TACCGTA3005.798938E-921.8148887
Produced by FastQC (version 0.11.5)