##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921532_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2201810
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.859759924789152	31.0	30.0	34.0	25.0	34.0
2	29.986549248118592	31.0	30.0	34.0	25.0	34.0
3	30.066717836688905	31.0	30.0	34.0	25.0	34.0
4	33.45125782878632	37.0	35.0	37.0	27.0	37.0
5	33.487428524713756	37.0	35.0	37.0	27.0	37.0
6	33.49069583660715	37.0	35.0	37.0	27.0	37.0
7	33.322055490709914	37.0	35.0	37.0	26.0	37.0
8	33.266382203732384	37.0	35.0	37.0	26.0	37.0
9	34.74433852148914	39.0	35.0	39.0	27.0	39.0
10-11	34.62104791058266	39.0	35.0	39.0	26.0	39.0
12-13	34.479456447195716	39.0	35.0	39.0	25.0	39.0
14-15	35.629927423347155	40.0	36.0	41.0	25.0	41.0
16-17	35.55261512119574	40.0	36.0	41.0	25.0	41.0
18-19	35.57063438716329	40.0	36.0	41.0	25.0	41.0
20-21	35.586990248931556	40.0	36.0	41.0	25.0	41.0
22-23	35.54840858203024	40.0	36.0	41.0	25.0	41.0
24-25	35.51136678459994	40.0	35.5	41.0	25.0	41.0
26-27	35.432711269364745	40.0	35.0	41.0	25.0	41.0
28-29	35.38513631966428	40.0	35.0	41.0	25.0	41.0
30-31	35.28883986356679	40.0	35.0	41.0	25.0	41.0
32-33	35.17924979902898	40.0	35.0	41.0	24.0	41.0
34-35	35.015794278343726	40.0	35.0	41.0	24.0	41.0
36-37	34.8748036842416	40.0	35.0	41.0	23.0	41.0
38-39	34.74869425608931	39.0	34.5	41.0	22.5	41.0
40-41	34.599194299235634	39.0	34.0	41.0	21.5	41.0
42-43	34.405730739709604	39.0	34.0	41.0	19.5	41.0
44-45	34.21891194063066	39.0	33.5	41.0	18.5	41.0
46-47	33.972495583179295	39.0	33.0	41.0	16.0	41.0
48-49	33.828777006190364	39.0	33.0	41.0	15.0	41.0
50-51	33.632916782102	38.0	33.0	40.5	13.0	41.0
52-53	33.44364318447096	38.0	33.0	40.0	12.5	41.0
54-55	33.239698929517075	38.0	33.0	40.0	9.0	41.0
56-57	33.001052770220866	37.5	32.5	40.0	8.0	41.0
58-59	32.75620262420463	37.0	32.0	40.0	7.5	41.0
60-61	32.45894082595683	36.5	32.0	40.0	7.0	41.0
62-63	32.1482389488648	36.0	32.0	39.5	3.5	41.0
64-65	31.83945254131828	35.5	31.0	39.0	2.0	41.0
66-67	31.795341332812548	35.0	32.0	39.0	2.0	41.0
68-69	31.550466661519387	35.0	31.0	38.5	2.0	40.5
70-71	31.25119197387604	35.0	31.0	37.5	2.0	40.0
72-73	30.924262992719626	35.0	31.0	37.0	2.0	39.5
74-75	30.62065255403509	35.0	31.0	36.5	2.0	39.0
76-77	30.341326454144543	35.0	31.0	36.0	2.0	39.0
78-79	30.064273029916297	35.0	30.5	35.5	2.0	37.5
80-81	29.821542503667438	35.0	30.0	35.0	2.0	37.0
82-83	29.619785085906596	35.0	30.0	35.0	2.0	36.5
84-85	29.416681275859407	35.0	30.0	35.0	2.0	36.0
86-87	29.263378538566	35.0	30.0	35.0	2.0	36.0
88-89	29.131475240824592	34.5	30.0	35.0	2.0	36.0
90-91	29.022643189012676	34.0	30.0	35.0	2.0	35.0
92-93	28.924178743851648	34.0	30.0	35.0	2.0	35.0
94-95	28.859270554680013	34.0	30.0	35.0	2.0	35.0
96-97	28.75993864139049	34.0	29.0	35.0	2.0	35.0
98-99	28.695261625662525	34.0	29.0	35.0	2.0	35.0
100-101	28.633007616461	34.0	29.0	35.0	2.0	35.0
102-103	27.666931978690258	33.5	28.0	34.5	2.0	35.0
104-105	28.477062280578252	34.0	29.0	35.0	2.0	35.0
106-107	28.79277344548349	34.5	30.0	35.0	2.0	35.0
108-109	28.829783223802238	35.0	30.0	35.0	2.0	35.0
110-111	28.774758039976202	35.0	30.0	35.0	2.0	35.0
112-113	28.702777714698364	35.0	30.0	35.0	2.0	35.0
114-115	28.584916046343693	35.0	30.0	35.0	2.0	35.0
116-117	28.524329983059392	35.0	30.0	35.0	2.0	35.0
118-119	28.365539033794924	35.0	30.0	35.0	2.0	35.0
120-121	28.197021541368237	35.0	29.5	35.0	2.0	35.0
122-123	28.06873163442804	34.5	29.0	35.0	2.0	35.0
124-125	27.234622878450004	34.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	85924.0
3	15303.0
4	9848.0
5	8316.0
6	8661.0
7	10067.0
8	11184.0
9	11976.0
10	12607.0
11	13121.0
12	13385.0
13	13875.0
14	15595.0
15	13303.0
16	9741.0
17	8666.0
18	7791.0
19	7185.0
20	7144.0
21	7427.0
22	8783.0
23	11702.0
24	16589.0
25	22750.0
26	27679.0
27	29612.0
28	34566.0
29	40916.0
30	46386.0
31	55192.0
32	69165.0
33	90054.0
34	124969.0
35	197017.0
36	378775.0
37	579831.0
38	176435.0
39	270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.79837373403732	17.10114480146007	12.21403248442862	24.886448980073993
2	16.663486655309484	19.291720613287904	38.5430096536059	25.50178307779671
3	18.91501942527357	24.417014649818103	28.793379779544043	27.874586145364283
4	13.167747237695188	15.185736916731294	35.87518530078127	35.77133054479225
5	14.265185523737525	37.15699427511696	33.58326976642968	14.994550434715844
6	33.441780028299135	34.717467581859836	17.41550927082926	14.425243119011766
7	29.529639640744843	31.222593436191865	20.921019570745795	18.326747352317497
8	27.622901215609375	33.488911585271666	19.56873944815078	19.31944775096818
9	27.696682274982887	14.507602069256583	18.989068121282386	38.806647534478145
10-11	26.229494045225664	24.85462336103429	27.14777826694781	21.768104326792237
12-13	26.543578955976926	22.645836788194483	27.14887062955332	23.66171362627527
14-15	23.962155882544028	23.750141411130883	24.51248938564644	27.77521332067865
16-17	24.106979375143403	26.73125895905697	24.95589474712458	24.20586691867505
18-19	24.21904447500563	25.900702303895763	26.455006860362374	23.425246360736235
20-21	25.364529201191942	24.880080856519214	26.094587403161455	23.660802539127385
22-23	25.109572837478662	24.5716491615207	25.936055359673833	24.382722641326808
24-25	24.50227544534625	25.070710711101952	26.045333479371468	24.38168036418033
26-27	24.242349198011375	25.048132040603132	26.1356639676978	24.573854793687694
28-29	24.299660383565726	25.0740212966859	26.21151369689347	24.414804622854906
30-31	24.623110097700334	25.003737431891267	25.994236402904704	24.3789160675037
32-33	24.194279515780398	25.179428324215458	25.986710931750324	24.639581228253824
34-35	24.5614449587241	25.172734505236672	26.025263969038413	24.240556567000816
36-37	24.661940175748395	24.97159924858428	25.862474018992604	24.50398655667472
38-39	24.653159331295555	25.163876762493214	25.796615576745314	24.386348329465914
40-41	24.661944711087884	25.109322548947215	25.978904559318856	24.24982818064604
42-43	24.558725891307578	25.064110342044522	25.900805406763816	24.47635835988408
44-45	24.490925537670385	25.088588283919684	25.85123712302973	24.5692490553802
46-47	24.62734388527659	25.196093535900832	25.679402350441045	24.49716022838154
48-49	24.650028911025608	25.36883993515751	25.376428369384072	24.604702784432806
50-51	24.63334086899424	25.510287313165787	25.30543851298311	24.550933304856866
52-53	24.74573197832791	25.366541327756252	25.47340660978698	24.414320084128857
54-55	24.660732741394867	25.2877958300759	25.558137268199655	24.49333416032958
56-57	24.559541749392096	25.394418268410483	25.446194789223668	24.599845192973753
58-59	24.668236566395287	25.203979048313606	25.41531345172331	24.712470933567797
60-61	24.58051661680078	25.368701275129535	25.61526311855981	24.435518989509877
62-63	24.518527370309318	25.512483347358206	25.502464500111778	24.466524782220702
64-65	24.569422231107556	25.19701210424921	25.575428919341352	24.658136745301878
66-67	24.48205228624839	25.449218168770503	25.50669156120223	24.562037983778872
68-69	24.6184746345232	25.382445346859107	25.447684520438692	24.551395498179005
70-71	24.49488707208992	25.170718718078284	25.67003802433412	24.66435618549768
72-73	24.742065705068867	25.395513639016244	25.429860203576087	24.4325604523388
74-75	24.468127399983597	25.5623938373226	25.421930264368207	24.547548498325593
76-77	24.553226530067523	25.270599192444642	25.487787286966103	24.688386990521728
78-79	24.521556452442777	25.34252098809238	25.48490631486193	24.651016244602907
80-81	24.49143783886919	25.543065205984767	25.282373009942056	24.683123945203985
82-83	24.697228827822105	25.414540193466472	25.334568902274217	24.5536620764372
84-85	24.466309169329726	25.439024445640644	25.32237220151584	24.772294183513786
86-87	24.335939079238507	25.54195774812314	25.453287587040528	24.668815585597823
88-89	24.496626764469525	25.334177683267928	25.41483460447672	24.75436094778583
90-91	24.38666657582096	25.378156604369224	25.4600312963455	24.77514552346431
92-93	24.54456316062389	25.567832475649883	25.37440795538971	24.513196408336515
94-95	24.42450529201892	25.274912212286715	25.495991211348933	24.80459128434543
96-97	24.465216503597322	25.303050983878084	25.496799213833228	24.734933298691363
98-99	24.45063349440862	25.29657967299075	25.393352027665856	24.85943480493478
100-101	24.317205288889664	25.37902127061352	25.499526812483065	24.804246628013747
102-103	24.413518922799266	25.20280632492751	25.635841346759815	24.747833405513404
104-105	24.26892476920376	25.430519493939034	25.49038310211128	24.810172634745918
106-107	24.27519239971448	25.44086656917703	25.434030636334505	24.849910394773985
108-109	24.302845306878883	25.368836076892332	25.46308913841346	24.86522947781533
110-111	24.375392171846837	25.442985281931108	25.472757980661598	24.70886456556046
112-113	24.326382373854745	25.448069698999177	25.544611433711754	24.68093649343432
114-115	24.08283232162011	25.641065238753953	25.581541118313105	24.69456132131284
116-117	24.150348817377484	25.635051110449485	25.46061403395221	24.75398603822082
118-119	24.2715728026528	25.327154525663214	25.49051836374816	24.910754307935825
120-121	23.953395349791286	25.481265719211997	25.654346565106458	24.910992365890262
122-123	24.045693673393362	25.49623383821405	25.578008159260037	24.880064329132555
124-125	24.056197256311982	25.52314766767897	25.60964725925283	24.811007816756213
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	119.0
1	141.5
2	184.0
3	209.0
4	201.0
5	192.5
6	204.5
7	257.5
8	351.0
9	415.0
10	401.5
11	411.0
12	512.0
13	635.5
14	724.5
15	801.5
16	902.0
17	1019.5
18	1150.0
19	1271.0
20	1416.5
21	1586.0
22	1909.5
23	2354.0
24	2840.0
25	3779.5
26	5274.0
27	6940.0
28	8348.5
29	10281.0
30	13125.0
31	15882.5
32	19255.0
33	22712.5
34	26475.5
35	30632.0
36	35324.0
37	39955.5
38	42408.0
39	45163.0
40	48410.0
41	50878.5
42	53738.5
43	59537.0
44	65584.0
45	69686.0
46	74318.0
47	78704.5
48	84307.0
49	90012.5
50	90221.5
51	89626.0
52	89453.0
53	89354.0
54	91151.5
55	90584.5
56	86366.0
57	79697.5
58	74489.5
59	69497.0
60	62327.5
61	55044.0
62	47506.0
63	37404.5
64	28061.5
65	21898.5
66	16807.0
67	12834.0
68	10158.0
69	8062.5
70	6731.0
71	5598.0
72	4485.0
73	3936.0
74	3130.5
75	2348.0
76	1609.0
77	928.0
78	559.0
79	368.5
80	220.5
81	98.5
82	62.5
83	45.0
84	33.5
85	23.0
86	16.5
87	15.0
88	16.0
89	11.5
90	10.5
91	13.0
92	11.0
93	8.0
94	9.5
95	9.5
96	8.5
97	10.0
98	9.0
99	8.0
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03710583565339425
2	0.025433620521298383
3	0.025161117444284477
4	0.02992992129202792
5	0.0325641177031624
6	0.047052197964402014
7	0.04192005668064002
8	0.03515289693479456
9	0.028749074624967643
10-11	0.0364699951403618
12-13	0.03656082949936643
14-15	0.03547081719131078
16-17	0.0374691730894128
18-19	0.05107161835035721
20-21	0.06217611873867408
22-23	0.055363541813326304
24-25	0.06683137963766174
26-27	0.04964097719603418
28-29	0.049595560016531856
30-31	0.050004314632052724
32-33	0.05811128117321658
34-35	0.040375872577561187
36-37	0.05286559694069879
38-39	0.062494038995190314
40-41	0.049845354503794605
42-43	0.05936025360953034
44-45	0.06001880271231397
46-47	0.06617283053487812
48-49	0.04961826860628301
50-51	0.05279747117144531
52-53	0.062494038995190314
54-55	0.04880075937524128
56-57	0.0456669739895813
58-59	0.0468932378361439
60-61	0.03358600424196456
62-63	0.04350965796322117
64-65	0.042237976937156245
66-67	0.03594769757608513
68-69	0.030815556292323135
70-71	0.050594737965582864
72-73	0.03254140911341124
74-75	0.04012607809029844
76-77	0.032813912190425155
78-79	0.033631421421466884
80-81	0.025206534623786796
82-83	0.017939785903415828
84-85	0.017939785903415828
86-87	0.017848951544411188
88-89	0.023208178725684776
90-91	0.0124897243631376
92-93	0.019733764493757407
94-95	0.015192046543525555
96-97	0.010968248849809929
98-99	0.011422420644833115
100-101	0.011422420644833115
102-103	0.006381113720075755
104-105	0.006290279361071119
106-107	0.009469481926233418
108-109	0.01317098205567238
110-111	0.0058361075660479335
112-113	0.00778904628464763
114-115	0.008243218079670815
116-117	0.007107788592112853
118-119	0.2491359381599684
120-121	0.36504058024988534
122-123	0.028612823086460685
124-125	0.002974825257401865
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2201810.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.249669333202604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.06414502045942	45.03581682846422
2	10.26061352501005	11.543122358752035
3	3.496957386041069	5.90108089911332
4	1.657480466094344	3.7293091257619753
5	1.0000053267377236	2.812498448021909
6	0.6754384020132947	2.2795912060916756
7	0.4911302341304727	1.9338139288564224
8	0.3575322808754201	1.608885806015048
9	0.27198105721420834	1.376896007987467
>10	1.5896293902998273	16.169560963879714
>50	0.09948655948382865	3.806087270019589
>100	0.03454225948540078	3.2936856491352193
>500	8.139170422264892E-4	0.28371455214389046
>1k	2.441751126678989E-4	0.2259369557576039
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.541717950231855E-5	0.0
2	0.0	0.0	0.0	4.541717950231855E-5	0.0
3	0.0	0.0	0.0	4.541717950231855E-5	0.0
4	0.0	0.0	0.0	4.541717950231855E-5	0.0
5	0.0	0.0	0.0	4.541717950231855E-5	0.0
6	0.0	0.0	0.0	4.541717950231855E-5	1.816687180092742E-4
7	0.0	0.0	0.0	4.541717950231855E-5	1.816687180092742E-4
8	0.0	0.0	0.0	4.541717950231855E-5	1.816687180092742E-4
9	0.0	0.0	0.0	4.541717950231855E-5	2.7250307701391126E-4
10-11	0.0	0.0	0.0	4.541717950231855E-5	2.7250307701391126E-4
12-13	0.0	0.0	0.0	4.541717950231855E-5	4.087546155208669E-4
14-15	0.0	0.0	0.0	4.541717950231855E-5	4.541717950231855E-4
16-17	0.0	0.0	0.0	4.541717950231855E-5	5.450061540278225E-4
18-19	0.0	0.0	0.0	4.541717950231855E-5	6.131319232813004E-4
20-21	0.0	0.0	0.0	9.083435900463709E-5	7.039662822859376E-4
22-23	0.0	0.0	0.0	1.3625153850695563E-4	7.49383461788256E-4
24-25	0.0	0.0	0.0	1.3625153850695563E-4	7.720920515394153E-4
26-27	0.0	0.0	0.0	2.9521166676507054E-4	8.175092310417338E-4
28-29	0.0	0.0	0.0	0.0011127208978068044	8.402178207928932E-4
30-31	0.0	0.0	0.0	0.0036106657704343243	8.629264105440524E-4
32-33	0.0	0.0	0.0	0.007970715002656906	9.08343590046371E-4
34-35	0.0	0.0	0.0	0.013852239748207157	9.537607695486895E-4
36-37	0.0	0.0	0.0	0.022731298340910436	9.537607695486895E-4
38-39	0.0	0.0	0.0	0.037310212961154686	9.764693592998488E-4
40-41	0.0	0.0	0.0	0.05309268283821038	0.0010445951285533266
42-43	0.0	4.541717950231855E-5	0.0	0.06896598707427071	0.0010445951285533266
44-45	0.0	4.541717950231855E-5	0.0	0.08983518105558608	0.0010900123080556452
46-47	0.0	4.541717950231855E-5	0.0	0.11233939349898492	0.0011354294875579637
48-49	0.0	4.541717950231855E-5	0.0	0.13779572261003448	0.0012262638465626008
50-51	0.0	4.541717950231855E-5	0.0	0.16574999659371153	0.0012716810260649193
52-53	0.0	4.541717950231855E-5	0.0	0.1935226018593793	0.0013625153850695564
54-55	0.0	4.541717950231855E-5	0.0	0.22206729917658652	0.001476058333825353
56-57	0.0	4.541717950231855E-5	0.0	0.25460870828999776	0.0015214755133276712
58-59	0.0	4.541717950231855E-5	0.0	0.29337227099522667	0.0015441841030788305
60-61	0.0	4.541717950231855E-5	0.0	0.33415689818830874	0.0015441841030788305
62-63	0.0	4.541717950231855E-5	0.0	0.3792788660238622	0.0015896012825811492
64-65	0.0	4.541717950231855E-5	0.0	0.42499125719294584	0.0016123098723323085
66-67	0.0	4.541717950231855E-5	0.0	0.4778795627233957	0.001657727051834627
68-69	0.0	4.541717950231855E-5	0.0	0.5396015096670467	0.0016804356415857863
70-71	0.0	4.541717950231855E-5	0.0	0.6099754293058892	0.0016804356415857863
72-73	0.0	4.541717950231855E-5	0.0	0.6815756127912944	0.001816687180092742
74-75	0.0	4.541717950231855E-5	0.0	0.7560370785853456	0.0018621043595950604
76-77	0.0	4.541717950231855E-5	0.0	0.8407174097674186	0.0018621043595950604
78-79	0.0	4.541717950231855E-5	0.0	0.9342540909524437	0.0018621043595950604
80-81	0.0	4.541717950231855E-5	0.0	1.0378279688074812	0.0018848129493462197
82-83	0.0	4.541717950231855E-5	0.0	1.1498040248704475	0.001907521539097379
84-85	0.0	4.541717950231855E-5	0.0	1.265913044268125	0.001907521539097379
86-87	0.0	4.541717950231855E-5	0.0	1.3915823799510403	0.001907521539097379
88-89	0.0	4.541717950231855E-5	0.0	1.5282880902530191	0.001907521539097379
90-91	0.0	4.541717950231855E-5	0.0	1.68951907748625	0.0019302301288485384
92-93	0.0	4.541717950231855E-5	0.0	1.8632624976723695	0.0020437730776043344
94-95	0.0	4.541717950231855E-5	0.0	2.0446360040148788	0.002134607436608972
96-97	0.0	4.541717950231855E-5	0.0	2.232617709974975	0.0021800246161112905
98-99	0.0	4.541717950231855E-5	0.0	2.436904183376404	0.0022708589751159274
100-101	0.0	4.541717950231855E-5	0.0	2.6769339770461578	0.0022708589751159274
102-103	0.0	4.541717950231855E-5	0.0	2.9336091670035107	0.002316276154618246
104-105	0.0	9.08343590046371E-5	0.0	3.2017749033749503	0.0023616933341205643
106-107	0.0	9.08343590046371E-5	0.0	3.474732152183885	0.0023616933341205643
108-109	0.0	9.08343590046371E-5	0.0	3.7558190761237347	0.0023844019238717234
110-111	0.0	9.08343590046371E-5	0.0	4.077736044436168	0.002520653462378679
112-113	0.0	9.08343590046371E-5	0.0	4.4205449153196685	0.0025433620521298386
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2485	0.0	70.16675	1
GTATCAA	4700	0.0	55.20504	1
TCAACGC	5780	0.0	43.541557	4
CAACGCA	5940	0.0	42.36776	5
ATCAACG	6335	0.0	40.008698	3
AACGCAG	6395	0.0	39.634224	6
TATCAAC	6660	0.0	38.50299	2
GTACATG	10945	0.0	38.386543	1
TACATGG	11380	0.0	36.59714	2
ACATGGG	11550	0.0	35.285805	3
ACGCAGA	7640	0.0	32.862503	7
CATGGGG	9410	0.0	31.739897	4
CGCAGAG	8080	0.0	30.772139	8
GCAGAGT	9000	0.0	26.899529	9
ATGGGGG	5055	0.0	26.246128	5
CAGAGTA	8460	0.0	24.502775	10-11
AGAGTAC	8040	0.0	23.711273	10-11
CATGGGT	2435	0.0	22.479172	4
ATGGGGA	3805	0.0	22.203184	5
TACCGTA	300	5.798938E-9	21.814888	7
>>END_MODULE