FastQCFastQC Report
Mon 15 Aug 2016
SRR2921532_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921532_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201810
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33660.15287422620480423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29600.1344348513268629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31150.079.616191
GTATCAA60700.058.8307271
TCAACGC83800.042.066854
CAACGCA86400.040.909035
ATCAACG88800.039.698223
AACGCAG90700.039.095456
TATCAAC91450.038.80922
GTACATG108000.034.5554581
ACGCAGA104450.033.9480827
TACATGG113050.032.5002562
CGCAGAG109750.032.1987958
ACATGGG113100.031.8006953
GCAGAGT117850.029.5313979
CATGGGG93400.029.2636244
AGAGTAC108100.027.62712510-11
CAGAGTA113000.026.1132510-11
GAGTACT62250.024.32238412-13
ATGGGGG48700.022.6042065
GTACTTT68000.022.30996114-15
CATGGGT21050.022.0650424
Produced by FastQC (version 0.11.5)