FastQCFastQC Report
Mon 15 Aug 2016
SRR2921531_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921531_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085207
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22900.10982123117752816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24800.070.310751
GTATCAA48050.061.6796231
TCAACGC63050.045.6710474
CAACGCA64800.044.529465
ATCAACG68650.042.2055053
AACGCAG68300.041.895126
TATCAAC71500.040.856032
GTACATG112700.037.333721
ACGCAGA78300.036.46687
TACATGG115950.035.6611022
ACATGGG119900.034.2877963
CGCAGAG84650.033.660988
CATGGGG99600.031.8979424
ATGGGGG53100.030.3637565
GCAGAGT93900.029.3940989
AGAGTAC84650.026.42281510-11
CAGAGTA89500.025.3232910-11
GAGTACT37100.021.32636312-13
GTATACG1703.9296816E-421.0042951
GTATATA6100.020.4877951
Produced by FastQC (version 0.11.5)