##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921531_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2085207 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.89209944144634 31.0 30.0 34.0 26.0 34.0 2 29.977655935357976 31.0 30.0 34.0 25.0 34.0 3 30.09420215834687 31.0 30.0 34.0 25.0 34.0 4 33.463693532584536 37.0 35.0 37.0 27.0 37.0 5 33.504900472710865 37.0 35.0 37.0 27.0 37.0 6 33.51060638104514 37.0 35.0 37.0 27.0 37.0 7 33.35829296563843 37.0 35.0 37.0 27.0 37.0 8 33.27838866836722 37.0 35.0 37.0 26.0 37.0 9 34.741132654935456 39.0 35.0 39.0 27.0 39.0 10-11 34.62527173561186 39.0 35.0 39.0 26.5 39.0 12-13 34.46738621153679 39.0 35.0 39.0 25.0 39.0 14-15 35.6179839699368 40.0 36.0 41.0 25.0 41.0 16-17 35.54652535695497 40.0 36.0 41.0 25.0 41.0 18-19 35.56811913637351 40.0 36.0 41.0 25.0 41.0 20-21 35.59156956599513 40.0 36.0 41.0 25.0 41.0 22-23 35.5573727212694 40.0 36.0 41.0 25.0 41.0 24-25 35.52566795526775 40.0 35.5 41.0 25.0 41.0 26-27 35.45407098671738 40.0 35.0 41.0 25.0 41.0 28-29 35.40854577027604 40.0 35.0 41.0 25.0 41.0 30-31 35.30447744516492 40.0 35.0 41.0 25.0 41.0 32-33 35.18913565895376 40.0 35.0 41.0 24.5 41.0 34-35 35.01822121257027 40.0 35.0 41.0 24.0 41.0 36-37 34.87526490175796 39.5 35.0 41.0 23.5 41.0 38-39 34.73947094940694 39.0 34.0 41.0 22.5 41.0 40-41 34.58537138039533 39.0 34.0 41.0 22.0 41.0 42-43 34.38040947493462 39.0 34.0 41.0 20.0 41.0 44-45 34.1817505408336 39.0 33.0 41.0 18.5 41.0 46-47 33.92104740680422 39.0 33.0 41.0 15.5 41.0 48-49 33.75530199160083 38.0 33.0 41.0 14.5 41.0 50-51 33.550586584449405 38.0 33.0 40.5 13.0 41.0 52-53 33.35046544539703 38.0 33.0 40.0 10.5 41.0 54-55 33.14100086945805 38.0 33.0 40.0 8.5 41.0 56-57 32.89644073705872 37.0 32.0 40.0 8.0 41.0 58-59 32.646237999392866 37.0 32.0 40.0 7.0 41.0 60-61 32.33107888089768 36.0 32.0 40.0 6.0 41.0 62-63 32.016880338498765 36.0 31.0 39.0 2.0 41.0 64-65 31.70473650817401 35.0 31.0 39.0 2.0 41.0 66-67 31.64871569105609 35.0 31.0 39.0 2.0 41.0 68-69 31.403589188027855 35.0 31.0 38.5 2.0 40.0 70-71 31.102451699039953 35.0 31.0 37.0 2.0 40.0 72-73 30.779134877256787 35.0 31.0 37.0 2.0 39.0 74-75 30.480326893205326 35.0 31.0 36.0 2.0 39.0 76-77 30.204491208786465 35.0 31.0 36.0 2.0 38.5 78-79 29.938493396578853 35.0 30.0 35.0 2.0 37.0 80-81 29.704486652883865 35.0 30.0 35.0 2.0 37.0 82-83 29.515092266619092 35.0 30.0 35.0 2.0 36.0 84-85 29.320273958412763 35.0 30.0 35.0 2.0 36.0 86-87 29.174569239408846 35.0 30.0 35.0 2.0 36.0 88-89 29.057836703981906 34.0 30.0 35.0 2.0 35.5 90-91 28.949396151077565 34.0 30.0 35.0 2.0 35.0 92-93 28.853006200343657 34.0 30.0 35.0 2.0 35.0 94-95 28.79354471762276 34.0 29.0 35.0 2.0 35.0 96-97 28.694767234140304 34.0 29.0 35.0 2.0 35.0 98-99 28.637126194185996 34.0 29.0 35.0 2.0 35.0 100-101 28.57253980060493 34.0 29.0 35.0 2.0 35.0 102-103 27.610350675016917 33.0 27.5 34.5 2.0 35.0 104-105 28.4196403522528 34.0 29.0 35.0 2.0 35.0 106-107 28.737811162153207 34.5 30.0 35.0 2.0 35.0 108-109 28.776842538894222 35.0 30.0 35.0 2.0 35.0 110-111 28.717229752250017 35.0 30.0 35.0 2.0 35.0 112-113 28.645385805821675 35.0 30.0 35.0 2.0 35.0 114-115 28.52735004246581 35.0 30.0 35.0 2.0 35.0 116-117 28.46610360506175 35.0 30.0 35.0 2.0 35.0 118-119 28.31035048318944 35.0 29.5 35.0 2.0 35.0 120-121 28.143090590046935 34.5 29.0 35.0 2.0 35.0 122-123 28.021239138368514 34.5 29.0 35.0 2.0 35.0 124-125 27.189100170870326 34.0 27.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 79253.0 3 14401.0 4 9264.0 5 7916.0 6 8476.0 7 9774.0 8 10678.0 9 11329.0 10 12080.0 11 13065.0 12 12827.0 13 13937.0 14 15735.0 15 13557.0 16 9451.0 17 8330.0 18 7574.0 19 7106.0 20 6620.0 21 6910.0 22 8312.0 23 11173.0 24 16344.0 25 22467.0 26 26344.0 27 28077.0 28 32970.0 29 39662.0 30 44018.0 31 52717.0 32 66119.0 33 86548.0 34 119462.0 35 189664.0 36 368598.0 37 543955.0 38 150305.0 39 189.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.884549586416064 16.967111897458434 12.341745735830155 24.806592780295347 2 16.768440977455512 19.20176641188711 38.13217197092309 25.897620639734292 3 19.098512743662706 24.026500448038128 28.785874230803916 28.089112577495246 4 13.376978056269936 15.086596253692028 35.372661555753524 36.16376413428451 5 14.528422452427762 37.02512911882578 33.233405964171986 15.213042464574478 6 33.873119082324244 34.28353601487749 17.094100340558732 14.74924456223953 7 29.8606566657775 31.175024516059697 20.532032079096197 18.432286739066605 8 28.127604107948056 32.994458482273714 19.479123239880888 19.398814169897342 9 27.932567743829573 14.329066608589446 18.719589212412473 39.018776435168505 10-11 26.561482035856326 24.668854315088538 26.68866308775061 22.081000561304528 12-13 26.769880565129796 22.403459152007866 26.858993164356015 23.967667118506323 14-15 24.220927566100862 23.47143105236545 24.271827892611345 28.035813488922344 16-17 24.3778441589781 26.469499629514225 24.72314313944058 24.4295130720671 18-19 24.470561252813507 25.51789922083007 26.32655372165319 23.68498580470323 20-21 25.629412835940574 24.471179489098958 26.033989528800838 23.865418146159627 22-23 25.410833884342725 24.08142975182025 25.840045411491257 24.667690952345765 24-25 24.830463516094177 24.59138606138129 25.89709831856375 24.68105210396078 26-27 24.51667926157832 24.63747046193338 25.97507407007569 24.87077620641261 28-29 24.538985245752585 24.733330310561065 26.05828816567863 24.669396278007724 30-31 24.859686047005383 24.639430026070386 25.798143189684314 24.702740737239917 32-33 24.558189394050267 24.71099579920341 25.796835379109357 24.933979427636967 34-35 24.858625601443897 24.727266272268835 25.870831272348 24.54327685393927 36-37 24.99944820041361 24.508730429108148 25.722521580162084 24.769299790316158 38-39 24.94621942857033 24.710271117421833 25.616083258034934 24.7274261959729 40-41 24.9954898545718 24.58511460087612 25.806596327590093 24.612799216961985 42-43 24.869210502891466 24.624534274225958 25.711469672071953 24.794785550810623 44-45 24.826961890347775 24.698095045236556 25.621804721262762 24.853138343152903 46-47 24.954039464396 24.76966945365148 25.472814061260557 24.803477020691957 48-49 25.063868243214877 24.94777905966082 25.138190270627664 24.85016242649664 50-51 24.967966042205415 25.18242142413824 25.053517881557962 24.796094652098382 52-53 25.115119803293418 24.998104530125016 25.163826181093675 24.7229494854879 54-55 24.898917613596836 24.939340972108603 25.30907675927974 24.852664655014824 56-57 24.84627726131714 25.03447246808478 25.192945066670763 24.926305203927317 58-59 24.978463264242254 24.83603653052603 25.141209257425828 25.04429094780589 60-61 24.895081646340234 25.014320131946704 25.409335094925446 24.681263126787616 62-63 24.79069056700001 25.09640552234821 25.303214109138604 24.809689801513173 64-65 24.879036765626626 24.85617549396419 25.360035042755136 24.904752697654047 66-67 24.770213731573563 24.983220888901894 25.312878249618482 24.93368712990606 68-69 24.87319774818965 24.99674989026382 25.235219196515306 24.89483316503122 70-71 24.762037738094207 24.801790078852136 25.435572419312585 25.000599763741064 72-73 24.988330697246237 25.08775363643298 25.159712337895034 24.76420332842575 74-75 24.77993818238954 25.217914873048407 25.168619106847075 24.833527837714982 76-77 24.863355275324697 24.8810092916254 25.17009380854939 25.08554162450051 78-79 24.81524319775946 25.005217085944476 25.22970769447018 24.949832021825888 80-81 24.81342388634759 25.200463190372396 25.035617113867314 24.950495809412704 82-83 24.987349130891285 25.04790542378133 25.09328067374092 24.87146477158646 84-85 24.77246247999229 25.13872893644107 25.043277096690797 25.04553148687585 86-87 24.630828010568703 25.241020330390736 25.113192438056835 25.01495922098373 88-89 24.70895427883979 25.067586884402925 25.124884637007106 25.098574199750185 90-91 24.69683823490618 25.094019356884097 25.107257128851963 25.101885279357756 92-93 24.751593804846625 25.235287374130067 25.062224462787874 24.950894358235427 94-95 24.711543257959182 24.97493870174321 25.24152376530538 25.07199427499223 96-97 24.701544240710945 25.05925229041308 25.158151995224802 25.08105147365117 98-99 24.67952107600821 25.017560242603537 25.070942420869795 25.231976260518458 100-101 24.598596668099944 25.1611956355174 25.169565074546764 25.070642621835894 102-103 24.695635033492735 24.849705934403445 25.35755239969325 25.097106632410572 104-105 24.516216443053608 25.146889200628276 25.150869872786352 25.186024483531767 106-107 24.50544298682628 25.14510035263409 25.101120011894356 25.24833664864528 108-109 24.584422099947815 25.10105864567026 25.159525892500845 25.154993361881083 110-111 24.57125468180511 25.148214697229083 25.228164185650677 25.052366435315133 112-113 24.58774271058898 25.149145462088345 25.218664345373842 25.04444748194883 114-115 24.339756118032156 25.373077061426123 25.277658541264493 25.009508279277227 116-117 24.361474137511383 25.434994207591018 25.112556901935275 25.09097475296233 118-119 24.48236648397649 25.154516660252764 25.130118976566447 25.232997879204305 120-121 24.260384221177095 25.257143352199694 25.365749884426574 25.116722542196634 122-123 24.264247698038265 25.269603551700897 25.27341716895284 25.192731581308 124-125 24.232120376476228 25.36229242851148 25.304310293147893 25.101276901864395 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 196.0 1 208.5 2 248.0 3 258.0 4 222.5 5 198.5 6 208.0 7 275.5 8 385.0 9 467.0 10 499.5 11 535.0 12 599.0 13 703.5 14 840.0 15 959.0 16 1050.5 17 1183.0 18 1371.0 19 1489.5 20 1651.0 21 1873.0 22 2149.5 23 2558.5 24 2999.0 25 3758.5 26 4974.0 27 6362.0 28 7543.0 29 9027.0 30 11069.5 31 13119.0 32 15557.0 33 18108.5 34 20984.5 35 23756.5 36 27114.5 37 31070.0 38 34206.5 39 37332.5 40 41014.5 41 44282.0 42 47482.0 43 53444.0 44 59384.0 45 63529.5 46 68369.0 47 73187.5 48 79278.5 49 85960.5 50 87307.0 51 87614.5 52 88397.0 53 88917.0 54 90875.0 55 90648.0 56 86603.5 57 80148.5 58 75150.0 59 69795.0 60 62620.0 61 55682.0 62 48315.5 63 38269.0 64 28792.0 65 22595.5 66 17340.0 67 13314.5 68 10622.0 69 8405.5 70 7059.5 71 5717.5 72 4441.5 73 3957.5 74 3233.5 75 2347.0 76 1617.5 77 958.0 78 550.0 79 346.0 80 198.0 81 100.0 82 60.5 83 32.0 84 25.0 85 20.5 86 21.5 87 20.5 88 14.5 89 10.0 90 10.5 91 11.0 92 8.0 93 6.5 94 6.0 95 9.0 96 7.5 97 7.0 98 10.0 99 9.0 100 20.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03572786778482903 2 0.025369183970704104 3 0.026999717534038587 4 0.031076051442374783 5 0.03323411057031748 6 0.047237516467190065 7 0.04119495090895053 8 0.0364472208274766 9 0.030021000313158356 10-11 0.037262487609143836 12-13 0.03671098360978071 14-15 0.03553604030678969 16-17 0.03747829352193811 18-19 0.05112202289748692 20-21 0.062367908797543836 22-23 0.055294270544842795 24-25 0.0653891915766636 26-27 0.04949148933415244 28-29 0.04927568342135817 30-31 0.049947079594495895 32-33 0.05742835123803057 34-35 0.04066742534434231 36-37 0.053567823242488634 38-39 0.06119296549455282 40-41 0.04877213629150487 42-43 0.058915014192835534 44-45 0.06104909488602331 46-47 0.06493360131632016 48-49 0.049563424638417196 50-51 0.05229696620047794 52-53 0.06191231853720038 54-55 0.048724179421995036 56-57 0.044839672991698186 58-59 0.046877839945866286 60-61 0.03496055787267163 62-63 0.04376064342772684 64-65 0.042969355080814516 66-67 0.03692678952257498 68-69 0.031747447615512514 70-71 0.050498583593859024 72-73 0.03251475752766991 74-75 0.04073936064860707 76-77 0.03335400274409207 78-79 0.03404937735198472 80-81 0.025656925187763132 82-83 0.017744041718639924 84-85 0.018511351630797326 86-87 0.017863933892414516 88-89 0.023163167973251575 90-91 0.012660613550597135 92-93 0.019950057716092456 94-95 0.015298241373638204 96-97 0.01278050572437173 98-99 0.011581583986625787 100-101 0.011533627117115952 102-103 0.006138479297259217 104-105 0.0063303067752985675 106-107 0.008848042424565043 108-109 0.0133799665932447 110-111 0.006737940166132187 112-113 0.007769012860593696 114-115 0.00872815025079045 116-117 0.0072175088612305635 118-119 0.2441724011093383 120-121 0.36080350775726344 122-123 0.027431329359627124 124-125 0.003932463299806685 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2085207.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.01492710954441 #Duplication Level Percentage of deduplicated Percentage of total 1 77.76871727428994 42.00671594973538 2 11.22352993737569 12.124763029582748 3 3.972613694714168 6.43741317463091 4 1.929921772915931 4.169783355647069 5 1.1751022262797375 3.1736530549381694 6 0.7731501217730159 2.50569884833829 7 0.5457675330227261 2.0635715460484896 8 0.39922464822026604 1.725127221916094 9 0.30362908591128046 1.476045264945187 >10 1.7789509254298737 17.333454599464808 >50 0.09670231130402372 3.5624076812239114 >100 0.03152691149306881 2.8435863426271992 >500 8.05539648724978E-4 0.26053518932557995 >1k 3.5801762165553957E-4 0.31724474157625354 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2290 0.10982123117752816 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.795686950983763E-5 2 0.0 0.0 0.0 0.0 4.795686950983763E-5 3 0.0 0.0 0.0 4.795686950983763E-5 4.795686950983763E-5 4 0.0 0.0 0.0 9.591373901967526E-5 4.795686950983763E-5 5 0.0 0.0 0.0 9.591373901967526E-5 4.795686950983763E-5 6 0.0 0.0 0.0 9.591373901967526E-5 4.795686950983763E-5 7 0.0 0.0 0.0 9.591373901967526E-5 4.795686950983763E-5 8 0.0 0.0 0.0 9.591373901967526E-5 4.795686950983763E-5 9 0.0 0.0 0.0 9.591373901967526E-5 4.795686950983763E-5 10-11 0.0 0.0 0.0 9.591373901967526E-5 4.795686950983763E-5 12-13 0.0 0.0 0.0 1.6784904328443173E-4 9.591373901967526E-5 14-15 0.0 0.0 0.0 1.9182747803935053E-4 1.6784904328443173E-4 16-17 0.0 0.0 0.0 1.9182747803935053E-4 1.9182747803935053E-4 18-19 0.0 0.0 0.0 1.9182747803935053E-4 2.3978434754918815E-4 20-21 0.0 0.0 0.0 1.9182747803935053E-4 3.356980865688634E-4 22-23 0.0 0.0 0.0 2.3978434754918815E-4 3.5967652132378223E-4 24-25 0.0 0.0 0.0 2.3978434754918815E-4 4.076333908336199E-4 26-27 0.0 0.0 0.0 4.076333908336199E-4 5.035471298532951E-4 28-29 0.0 0.0 0.0 0.0010070942597065903 5.27525564608214E-4 30-31 0.0 0.0 0.0 0.0031171965181394463 5.27525564608214E-4 32-33 0.0 0.0 0.0 0.0077929912953486145 5.754824341180516E-4 34-35 0.0 0.0 0.0 0.014123298070647182 5.754824341180516E-4 36-37 0.0 0.0 0.0 0.024410046580507355 5.754824341180516E-4 38-39 0.0 0.0 0.0 0.0392526976938021 5.754824341180516E-4 40-41 0.0 0.0 0.0 0.05867522984528634 5.754824341180516E-4 42-43 0.0 0.0 0.0 0.07689884025902465 5.754824341180516E-4 44-45 0.0 0.0 0.0 0.09859932371222618 5.994608688729704E-4 46-47 0.0 0.0 0.0 0.12300937029273354 6.713961731377269E-4 48-49 0.0 0.0 0.0 0.15094424678221396 8.152667816672398E-4 50-51 0.0 0.0 0.0 0.18137288048620592 8.152667816672398E-4 52-53 0.0 0.0 0.0 0.21184947105970775 9.11180520686915E-4 54-55 0.0 0.0 0.0 0.2441724011093383 9.591373901967526E-4 56-57 0.0 0.0 0.0 0.28117112593617805 0.0010310726944615092 58-59 0.0 0.0 0.0 0.32332521423532534 0.0010790295639713466 60-61 0.0 0.0 0.0 0.37097036409334905 0.0011030079987262656 62-63 0.0 0.0 0.0 0.42034196125372686 0.0011030079987262656 64-65 0.0 0.0 0.0 0.46988140745738916 0.0011030079987262656 66-67 0.0 0.0 0.0 0.5265184703485073 0.0011030079987262656 68-69 0.0 0.0 0.0 0.5917158344471316 0.0011030079987262656 70-71 0.0 0.0 0.0 0.6666724214910078 0.0011269864334811845 72-73 0.0 0.0 0.0 0.7430437361854243 0.0012468786072557785 74-75 0.0 0.0 0.0 0.8212613903559695 0.0012468786072557785 76-77 0.0 0.0 0.0 0.9142257819007897 0.0012468786072557785 78-79 0.0 0.0 0.0 1.0192273476925793 0.0012468786072557785 80-81 0.0 0.0 0.0 1.1301755653035885 0.0012468786072557785 82-83 0.0 0.0 0.0 1.2459434483003367 0.0012468786072557785 84-85 0.0 0.0 0.0 1.3696961500704727 0.0012468786072557785 86-87 0.0 0.0 0.0 1.5041672121760574 0.001294835476765616 88-89 0.0 0.0 0.0 1.657581237738028 0.0013667707810303725 90-91 0.0 0.0 0.0 1.8305376876252573 0.0013907492157852913 92-93 0.0 0.0 0.0 2.016418513845388 0.001438706085295129 94-95 0.0 0.0 0.0 2.2073587897988065 0.0016305335633344795 96-97 0.0 0.0 0.0 2.401584111313649 0.00182236104137383 98-99 0.0 0.0 0.0 2.6197159322791457 0.0020621453889230184 100-101 0.0 0.0 0.0 2.871993044335646 0.0020621453889230184 102-103 0.0 0.0 0.0 3.148536332364125 0.002086123823677937 104-105 0.0 0.0 0.0 3.4276213344766253 0.002110102258432856 106-107 0.0 0.0 0.0 3.719534799183007 0.002110102258432856 108-109 0.0 0.0 0.0 4.027513815175184 0.0021580591279426933 110-111 0.0 0.0 0.0 4.365585766784784 0.0022539728669623686 112-113 0.0 0.0 0.0 4.7276601315840585 0.0022539728669623686 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2480 0.0 70.31075 1 GTATCAA 4805 0.0 61.679623 1 TCAACGC 6305 0.0 45.671047 4 CAACGCA 6480 0.0 44.52946 5 ATCAACG 6865 0.0 42.205505 3 AACGCAG 6830 0.0 41.89512 6 TATCAAC 7150 0.0 40.85603 2 GTACATG 11270 0.0 37.33372 1 ACGCAGA 7830 0.0 36.4668 7 TACATGG 11595 0.0 35.661102 2 ACATGGG 11990 0.0 34.287796 3 CGCAGAG 8465 0.0 33.66098 8 CATGGGG 9960 0.0 31.897942 4 ATGGGGG 5310 0.0 30.363756 5 GCAGAGT 9390 0.0 29.394098 9 AGAGTAC 8465 0.0 26.422815 10-11 CAGAGTA 8950 0.0 25.32329 10-11 GAGTACT 3710 0.0 21.326363 12-13 GTATACG 170 3.9296816E-4 21.004295 1 GTATATA 610 0.0 20.487795 1 >>END_MODULE