FastQCFastQC Report
Mon 15 Aug 2016
SRR2921531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085207
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38940.18674404987130774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34210.16406045059315455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30950.084.761521
GTATCAA65250.063.68821
TCAACGC88600.046.851764
CAACGCA89950.045.8507275
ATCAACG95200.043.666193
AACGCAG95150.043.6451266
TATCAAC97050.043.080312
ACGCAGA110250.037.340957
CGCAGAG116050.035.371338
GTACATG109150.033.5937231
GCAGAGT124750.032.379999
TACATGG113450.032.1280362
ACATGGG117050.031.0373953
CATGGGG97000.029.7166944
AGAGTAC116650.028.0246910-11
CAGAGTA122400.027.21851710-11
GAGTACT67950.026.26700212-13
GTACTTT72300.024.72776814-15
AGTACTT72250.024.66253312-13
ATGGGGG51000.023.4552485
Produced by FastQC (version 0.11.5)