##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921531_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2085207
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.250119052928557	31.0	31.0	34.0	28.0	34.0
2	31.31766102837752	33.0	31.0	34.0	28.0	34.0
3	31.405516574613454	33.0	31.0	34.0	28.0	34.0
4	34.908497813406534	37.0	35.0	37.0	32.0	37.0
5	34.567467402516876	37.0	35.0	37.0	31.0	37.0
6	34.5291220487942	37.0	35.0	37.0	31.0	37.0
7	34.37548598292639	37.0	35.0	37.0	30.0	37.0
8	34.33832852086148	37.0	35.0	37.0	30.0	37.0
9	35.90822397968164	39.0	37.0	39.0	30.0	39.0
10-11	35.783058708320084	39.0	37.0	39.0	30.0	39.0
12-13	35.68846426278063	39.0	36.0	39.0	30.0	39.0
14-15	36.8710684358915	40.0	37.0	41.0	30.0	41.0
16-17	36.856044268027105	40.0	37.0	41.0	30.0	41.0
18-19	36.88180669832779	40.0	37.0	41.0	30.0	41.0
20-21	36.86924943183099	40.0	37.0	41.0	30.0	41.0
22-23	36.81235771796277	40.0	37.0	41.0	30.0	41.0
24-25	36.74395443713742	40.0	37.0	41.0	30.0	41.0
26-27	36.62100045702897	40.0	36.0	41.0	30.0	41.0
28-29	36.436595503468	40.0	36.0	41.0	29.0	41.0
30-31	36.385184060863025	40.0	36.0	41.0	29.5	41.0
32-33	36.254704688791094	40.0	35.5	41.0	29.0	41.0
34-35	36.119159392808484	40.0	35.0	41.0	28.5	41.0
36-37	35.97585539469223	40.0	35.0	41.0	28.0	41.0
38-39	35.80162880711603	40.0	35.0	41.0	27.0	41.0
40-41	35.59629044022968	39.5	35.0	41.0	26.5	41.0
42-43	35.41529162332564	39.0	35.0	41.0	26.0	41.0
44-45	35.18558924845351	39.0	35.0	41.0	25.0	41.0
46-47	34.89207881999245	39.0	34.5	41.0	24.0	41.0
48-49	34.61999480147534	39.0	34.0	41.0	23.0	41.0
50-51	34.39015814736858	39.0	34.0	41.0	22.0	41.0
52-53	34.179830587562776	38.0	34.0	41.0	22.0	41.0
54-55	33.95125807653629	38.0	33.5	40.0	21.0	41.0
56-57	33.683562351363676	37.5	33.0	40.0	20.0	41.0
58-59	33.52601204580648	37.0	33.0	40.0	20.0	41.0
60-61	33.39694572289466	37.0	33.0	40.0	20.0	41.0
62-63	33.1252633431597	36.0	33.0	40.0	19.5	41.0
64-65	32.821682211885914	36.0	33.0	39.0	18.0	41.0
66-67	32.51605859753972	35.0	33.0	39.0	18.0	41.0
68-69	32.20139415415352	35.0	32.0	38.5	17.0	40.5
70-71	31.896616498985473	35.0	32.0	37.0	14.5	40.0
72-73	31.586985848407373	35.0	32.0	37.0	13.0	39.0
74-75	31.30822239710494	35.0	32.0	36.5	11.5	39.0
76-77	31.026081583267274	35.0	32.0	36.0	9.0	38.5
78-79	30.758895639617553	35.0	31.0	35.5	7.5	37.0
80-81	30.547402967666997	35.0	31.0	35.0	7.0	37.0
82-83	30.369445335642936	35.0	31.0	35.0	7.0	36.5
84-85	30.189507324692464	35.0	31.0	35.0	6.5	36.0
86-87	30.053362807625334	35.0	31.0	35.0	3.5	36.0
88-89	29.955021491871072	35.0	31.0	35.0	2.0	35.5
90-91	29.861448048083474	35.0	31.0	35.0	2.0	35.0
92-93	29.922336007887946	35.0	31.0	35.0	2.0	35.0
94-95	29.89322115262418	35.0	31.0	35.0	2.0	35.0
96-97	29.87596890860236	35.0	31.0	35.0	2.0	35.0
98-99	29.835695209156693	35.0	31.0	35.0	2.0	35.0
100-101	29.78623872833728	35.0	31.0	35.0	2.0	35.0
102-103	28.901014863272565	33.5	29.5	35.0	2.0	35.0
104-105	29.60566265123798	34.5	31.0	35.0	2.0	35.0
106-107	29.71105003004498	35.0	31.0	35.0	2.0	35.0
108-109	29.717035766712847	35.0	31.0	35.0	2.0	35.0
110-111	29.683743628330426	35.0	31.0	35.0	2.0	35.0
112-113	29.640841892435617	35.0	31.0	35.0	2.0	35.0
114-115	29.579741483699223	35.0	31.0	35.0	2.0	35.0
116-117	29.508180482800988	35.0	31.0	35.0	2.0	35.0
118-119	29.41591650133536	35.0	31.0	35.0	2.0	35.0
120-121	29.294906932501185	35.0	31.0	35.0	2.0	35.0
122-123	29.177632484448786	35.0	31.0	35.0	2.0	35.0
124-125	28.27523958053085	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34907.0
3	12772.0
4	7182.0
5	6013.0
6	6520.0
7	7651.0
8	9013.0
9	9947.0
10	11412.0
11	12552.0
12	13055.0
13	15304.0
14	19170.0
15	16103.0
16	8835.0
17	7572.0
18	6811.0
19	6090.0
20	5920.0
21	6401.0
22	7552.0
23	9766.0
24	13755.0
25	19249.0
26	24064.0
27	26546.0
28	31356.0
29	37050.0
30	43197.0
31	51533.0
32	64567.0
33	82826.0
34	115544.0
35	184393.0
36	374830.0
37	596237.0
38	179101.0
39	411.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.93240810008038	17.529191230770856	12.575732985826685	24.96266768332208
2	16.87553832489598	19.688207975169433	37.237599584111045	26.198654115823537
3	18.995831754452443	24.234822357912712	29.136611969436068	27.63273391819878
4	13.528539018744507	15.50701911302365	35.08711991800478	35.877321950227056
5	14.458374006749189	37.098616344357325	33.08757396165866	15.355435687234822
6	33.76547065025418	34.846989141164855	16.52909647852936	14.858443730051604
7	29.55379386492945	31.54792682119618	20.366488781533203	18.531790532341166
8	28.18259077118458	33.16820761621388	19.064370733373043	19.584830879228498
9	27.972563570956176	14.058484176621771	18.606779207437878	39.362173044984175
10-11	26.66629896788551	24.642189118673983	26.500522771745388	22.190989141695123
12-13	26.98713776865057	22.369510866880535	26.9280278087782	23.715323555690695
14-15	24.389213966825267	23.197016412798945	24.663176255226865	27.75059336514893
16-17	24.247870887010308	26.205146418560126	25.101770317911885	24.44521237651768
18-19	24.29591531847893	24.943707276880584	27.221026692942203	23.539350711698287
20-21	25.356448325454895	23.962430983215338	27.066186805710274	23.614933885619497
22-23	25.126151139640836	23.568212742548077	26.92121195261964	24.384424165191447
24-25	24.59663358899818	24.12226826246552	26.95419089055495	24.326907257981354
26-27	24.286156560784097	24.191696061172227	27.06563098246833	24.456516395575346
28-29	24.429992814954872	24.228375076622893	27.04663468410263	24.294997424319604
30-31	24.64843743441892	24.21545539383186	26.813803332184506	24.32230383956472
32-33	24.322990411069288	24.259582215775882	26.847351244949408	24.57007612820542
34-35	24.525400285552497	24.362069821494913	26.824762241450394	24.287767651502197
36-37	24.792906801645472	24.1196755386006	26.66587684942542	24.42154081032851
38-39	24.759268599617073	24.329263042570524	26.518000681619608	24.39346767619279
40-41	24.7784378465281	24.21224210635367	26.615252608193508	24.394067438924722
42-43	24.730575022685894	24.341509528075832	26.371105747924695	24.556809701313576
44-45	24.766651789236782	24.395611406008257	26.2060611176971	24.631675687057857
46-47	24.85804056285415	24.50024306439084	25.9432565160323	24.698459856722714
48-49	25.061380688341515	24.794632220730158	25.39633924783264	24.74764784309568
50-51	24.96114824677866	25.023071226295635	25.28428931029984	24.731491216625866
52-53	25.17851198282371	24.82604495345689	25.344696253814636	24.650746809904764
54-55	24.920502252606056	24.87783644293298	25.470960748174594	24.730700556286372
56-57	24.882832801970707	24.937851798394473	25.32113801541635	24.858177384218468
58-59	24.932182508495323	24.661919821989727	25.483188385011967	24.922709284502982
60-61	24.875183743775374	24.848058377816777	25.627019262847195	24.649738615560654
62-63	24.807257965291683	24.910653319048418	25.51519595110414	24.766892764555756
64-65	24.827556269502672	24.685193210605952	25.656552478030974	24.8306980418604
66-67	24.805918607160844	24.829516295354807	25.568598767356534	24.795966330127822
68-69	24.910723729607486	24.835892118720352	25.412311382967317	24.841072768704844
70-71	24.76847477300743	24.636356223649436	25.64629928964396	24.948869713699175
72-73	25.03001485513485	24.97799550172894	25.253537387980916	24.738452255155288
74-75	24.82937040328975	25.120048695809004	25.20984238617139	24.84073851472986
76-77	24.879963950214268	24.83701213658533	25.20053172954315	25.08249218365726
78-79	24.827751957061576	24.932665856426265	25.27635841792526	24.963223768586897
80-81	24.828845440327406	25.083577628561088	25.131809097399987	24.95576783371152
82-83	24.955737229624198	24.980269703737402	25.064946307934587	24.999046758703813
84-85	24.755961157150846	25.02769898422669	25.03681206994629	25.179527788676175
86-87	24.624463377789947	25.037909192046783	25.195133713988067	25.142493716175203
88-89	24.69704957004375	24.971735179376683	25.08184446362077	25.249370786958803
90-91	24.642966174026213	24.989549966269713	25.133464257527198	25.234019602176865
92-93	24.718780754982667	25.103475828621967	25.10954289342877	25.0682005229666
94-95	24.642859883474433	24.857532435890796	25.239923914982764	25.259683765652007
96-97	24.62284469838846	24.906949095724094	25.196784498373976	25.273421707513467
98-99	24.60175128005734	24.91565996958028	25.071906934741957	25.41068181562043
100-101	24.5746172802557	24.971030424768795	25.14892472035247	25.305427574623035
102-103	24.62512538184061	24.78012767066618	25.26563685102683	25.329110096466383
104-105	24.48778068831763	25.00549123273139	25.073208443357814	25.433519635593164
106-107	24.526376821046593	25.009279676501368	25.0278869864886	25.43645651596344
108-109	24.584528210206553	24.888207011953607	25.017981612826556	25.509283165013287
110-111	24.57432464674023	25.00865030538036	25.072937120972156	25.344087926907257
112-113	24.526295191225326	25.018501190167296	25.03562348411344	25.41958013449394
114-115	24.286239769340778	25.19387057262917	25.08994539492047	25.429944263109583
116-117	24.30735193152499	25.18249794306875	24.957560430374045	25.552589695032214
118-119	24.274561038744274	24.974735956573184	25.04279419600869	25.707908808673853
120-121	24.084515384429082	25.0369584552193	25.211073844252454	25.667452316099165
122-123	24.14651785230432	25.046842186790386	25.09712530207036	25.709514658834937
124-125	24.091898765629892	25.24102907506906	24.98933902941756	25.677733129883478
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	50.0
1	45.0
2	97.0
3	160.0
4	124.5
5	97.5
6	208.5
7	453.5
8	753.0
9	898.5
10	751.5
11	608.5
12	664.0
13	758.5
14	920.0
15	1084.0
16	1271.0
17	1557.0
18	1751.0
19	1983.0
20	2265.0
21	2514.5
22	2885.0
23	3406.0
24	3918.0
25	4507.0
26	5642.5
27	6977.5
28	8050.0
29	9603.5
30	11642.0
31	13513.0
32	15855.0
33	18320.5
34	20789.5
35	23368.5
36	26829.0
37	30589.0
38	33247.0
39	36499.0
40	40066.0
41	42957.5
42	46475.0
43	52339.5
44	58234.0
45	62937.5
46	68260.5
47	73334.0
48	79577.5
49	86145.0
50	87521.5
51	87893.5
52	88415.5
53	89347.5
54	91919.0
55	91159.0
56	86655.0
57	79987.0
58	75013.5
59	70162.0
60	62838.5
61	55657.5
62	48041.0
63	37664.0
64	28232.0
65	22108.5
66	16964.0
67	13051.5
68	10299.0
69	8070.0
70	6759.5
71	5467.5
72	4290.5
73	3915.0
74	3176.5
75	2296.5
76	1541.5
77	842.5
78	469.5
79	251.5
80	130.5
81	53.5
82	25.5
83	14.0
84	6.5
85	3.5
86	1.5
87	1.0
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12281754281469418
2	0.0016784904328443172
3	0.01903887719540554
4	0.05673297663013792
5	0.024553917189036868
6	0.021724461887956445
7	0.016353292502854635
8	0.006186436166769055
9	0.004603859472944413
10-11	0.008008797208142885
12-13	0.00527525564608214
14-15	0.0031651533876492837
16-17	0.011629540856135625
18-19	0.005946651819219866
20-21	0.014267168679176696
22-23	0.009975028858046228
24-25	0.015154370765108692
26-27	0.009999007292801145
28-29	0.015394155112657881
30-31	0.022947362060457308
32-33	0.014530931461480802
34-35	0.02285144832143763
36-37	0.01613748659006036
38-39	0.022371879626339256
40-41	0.011461691812851194
42-43	0.01011889946657574
44-45	0.01887102815212111
46-47	0.01767210641437517
48-49	0.022611663973888443
50-51	0.01721651615403171
52-53	0.0226835992781532
54-55	0.019158769369180133
56-57	0.022659620843398284
58-59	0.01839145945702273
60-61	0.021220914758103154
62-63	0.023522844494575357
64-65	0.01901489876065062
66-67	0.012324915464028273
68-69	0.025249291796929512
70-71	0.01616146502481528
72-73	0.01947048902099408
74-75	0.020525540150210506
76-77	0.014986521721824261
78-79	0.030716374921051004
80-81	0.02246779336535893
82-83	0.010334705379370009
84-85	0.014051362766382426
86-87	0.01393147059260783
88-89	0.02206015997452531
90-91	0.013691686245058645
92-93	0.008584279642260936
94-95	0.008200624686182236
96-97	0.0027814984315705825
98-99	0.00222999443220745
100-101	0.012516742942067621
102-103	0.003668700517502579
104-105	0.003117196518139446
106-107	2.3978434754918818E-4
108-109	0.0210290872800638
110-111	9.831158249516713E-4
112-113	0.009879115119026552
114-115	0.0025417140840213945
116-117	0.008800085555055206
118-119	0.01093416624824298
120-121	0.004579881038189494
122-123	6.713961731377268E-4
124-125	0.023978434754918815
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2085207.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.78647681348808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.23900764896219	40.77175086358771
2	11.464966863240724	12.103904149877314
3	4.030274285814777	6.38231940420477
4	2.010912244465259	4.245958902656987
5	1.1969871355151802	3.1592366837457795
6	0.7621601263767519	2.4139048703490937
7	0.5635203064562743	2.0822376113478676
8	0.41075551625832046	1.7345869227985729
9	0.31697856272304586	1.5058963396397556
>10	1.8613188679691484	17.74703999466411
>50	0.10553472347800226	3.7675125373109823
>100	0.03630742491906808	3.285331718080136
>500	9.116384437403639E-4	0.28815562325783634
>1k	3.646553774961384E-4	0.5121643784791415
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3894	0.18674404987130774	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3421	0.16406045059315455	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.795686950983763E-5
2	0.0	0.0	0.0	0.0	4.795686950983763E-5
3	0.0	0.0	0.0	0.0	4.795686950983763E-5
4	0.0	0.0	0.0	0.0	4.795686950983763E-5
5	0.0	0.0	0.0	0.0	4.795686950983763E-5
6	0.0	0.0	0.0	0.0	4.795686950983763E-5
7	0.0	0.0	0.0	0.0	4.795686950983763E-5
8	0.0	0.0	0.0	0.0	4.795686950983763E-5
9	0.0	0.0	0.0	0.0	9.591373901967526E-5
10-11	0.0	0.0	0.0	0.0	9.591373901967526E-5
12-13	0.0	0.0	0.0	4.795686950983763E-5	2.3978434754918815E-4
14-15	0.0	0.0	0.0	4.795686950983763E-5	2.63762782304107E-4
16-17	0.0	0.0	0.0	7.193530426475645E-5	2.877412170590258E-4
18-19	0.0	0.0	0.0	9.591373901967526E-5	3.356980865688634E-4
20-21	0.0	0.0	0.0	9.591373901967526E-5	3.356980865688634E-4
22-23	0.0	0.0	0.0	1.438706085295129E-4	4.3161182558853865E-4
24-25	0.0	0.0	0.0	1.438706085295129E-4	4.795686950983763E-4
26-27	0.0	0.0	0.0	3.5967652132378223E-4	4.795686950983763E-4
28-29	0.0	0.0	0.0	8.872020859319962E-4	4.795686950983763E-4
30-31	0.0	0.0	0.0	0.0030932180833845276	4.795686950983763E-4
32-33	0.0	0.0	0.0	0.0077929912953486145	4.795686950983763E-4
34-35	0.0	0.0	0.0	0.014195233374911938	4.795686950983763E-4
36-37	0.0	0.0	0.0	0.02450596031952703	5.27525564608214E-4
38-39	0.0	0.0	0.0	0.0397562448236554	5.754824341180516E-4
40-41	0.0	0.0	0.0	0.05965834567023802	5.754824341180516E-4
42-43	0.0	0.0	0.0	0.07862528756137879	5.994608688729704E-4
44-45	0.0	0.0	0.0	0.10097318875296313	6.47417738382808E-4
46-47	0.0	0.0	0.0	0.1263903295931771	6.713961731377268E-4
48-49	0.0	0.0	0.0	0.15478079634300096	7.673099121574021E-4
50-51	0.0	0.0	0.0	0.18544921439454212	8.152667816672398E-4
52-53	0.0	0.0	0.0	0.21666913644544644	8.152667816672398E-4
54-55	0.0	0.0	0.0	0.24973539797247946	8.152667816672398E-4
56-57	0.0	0.0	0.0	0.2876213248852512	8.152667816672398E-4
58-59	0.0	0.0	0.0	0.3308304643136149	8.152667816672398E-4
60-61	0.0	0.0	0.0	0.3799622771264436	8.392452164221586E-4
62-63	0.0	0.0	0.0	0.43094042941540095	8.632236511770774E-4
64-65	0.0	0.0	0.0	0.4818946032696034	8.632236511770774E-4
66-67	0.0	0.0	0.0	0.5394428466814085	8.632236511770774E-4
68-69	0.0	0.0	0.0	0.6059110678220436	8.632236511770774E-4
70-71	0.0	0.0	0.0	0.6816109863433222	8.632236511770774E-4
72-73	0.0	0.0	0.0	0.7594929424272986	9.111805206869151E-4
74-75	0.0	0.0	0.0	0.8387656477270602	9.111805206869151E-4
76-77	0.0	0.0	0.0	0.9331207884876658	9.111805206869151E-4
78-79	0.0	0.0	0.0	1.0408798742762708	9.111805206869151E-4
80-81	0.0	0.0	0.0	1.1545856118840958	9.351589554418339E-4
82-83	0.0	0.0	0.0	1.2723437049655022	0.0010070942597065903
84-85	0.0	0.0	0.0	1.3989258620367186	0.0010070942597065903
86-87	0.0	0.0	0.0	1.5356269185745108	0.0011030079987262656
88-89	0.0	0.0	0.0	1.6924698603064348	0.0012468786072557785
90-91	0.0	0.0	0.0	1.8683516792337644	0.0012468786072557785
92-93	0.0	0.0	0.0	2.0581170118841916	0.0013427923462754536
94-95	0.0	0.0	0.0	2.2543325434836925	0.0013427923462754536
96-97	0.0	0.0	0.0	2.453041832297705	0.0013907492157852913
98-99	0.0	0.0	0.0	2.674818375345949	0.0014866629548049666
100-101	0.0	0.0	0.0	2.93285031174363	0.0014866629548049666
102-103	0.0	0.0	0.0	3.213565847419465	0.0014866629548049666
104-105	0.0	0.0	0.0	3.4992928759590773	0.0015825766938246419
106-107	0.0	0.0	0.0	3.7958581570079133	0.0016065551285795606
108-109	0.0	0.0	0.0	4.112061776121028	0.0016784904328443172
110-111	0.0	0.0	0.0	4.459821015371615	0.0017264473023541548
112-113	0.0	0.0	0.0	4.831726538420407	0.0017264473023541548
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3095	0.0	84.76152	1
GTATCAA	6525	0.0	63.6882	1
TCAACGC	8860	0.0	46.85176	4
CAACGCA	8995	0.0	45.850727	5
ATCAACG	9520	0.0	43.66619	3
AACGCAG	9515	0.0	43.645126	6
TATCAAC	9705	0.0	43.08031	2
ACGCAGA	11025	0.0	37.34095	7
CGCAGAG	11605	0.0	35.37133	8
GTACATG	10915	0.0	33.593723	1
GCAGAGT	12475	0.0	32.37999	9
TACATGG	11345	0.0	32.128036	2
ACATGGG	11705	0.0	31.037395	3
CATGGGG	9700	0.0	29.716694	4
AGAGTAC	11665	0.0	28.02469	10-11
CAGAGTA	12240	0.0	27.218517	10-11
GAGTACT	6795	0.0	26.267002	12-13
GTACTTT	7230	0.0	24.727768	14-15
AGTACTT	7225	0.0	24.662533	12-13
ATGGGGG	5100	0.0	23.455248	5
>>END_MODULE