FastQCFastQC Report
Mon 15 Aug 2016
SRR2921530_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921530_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1834752
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.071.366951
GTATCAA41400.060.238881
TCAACGC52350.046.9361044
CAACGCA53600.046.1732445
AACGCAG56450.043.943886
ATCAACG57100.042.928593
TATCAAC60650.040.3177722
ACGCAGA66150.036.959537
GTACATG94000.036.851771
TACATGG96700.035.6851122
ACATGGG100100.034.473033
CGCAGAG71300.034.370568
CATGGGG79550.032.832054
GCAGAGT75350.031.1803469
CCCTATA3100.028.7882372
GTATAGA5450.028.394881
AGAGTAC69150.026.96611410-11
CAGAGTA72200.026.73317510-11
ATGGGGG40750.026.7168545
ATGGGGC31050.022.6090455
Produced by FastQC (version 0.11.5)